The new Sepapure® columns and media represent high-quality alternatives for common protein purification tasks like size-exclusion (SEC), affinity (AC) and ion-exchange (IEX) chromatography.
Sepapure FPLC columns come as prepacked ready-to-use 1 ml or 5 ml cartridges and can also be purchased as bulk material. The media for affinity as well as ion-exchange chromatography are based on agarose, which is well-known in FPLC chromatography.
For affinity chromatography the agarose solid phase is functionalized with ligands for the purification of recombinantly expressed proteins with either His- or GST-tags. Antibodies can be purified via Protein A or Protein G affinity. The ion-exchange columns can be purchased as weak and strong anion or cation exchangers. The basis of our Sepapure desalting column is dextran with a 5 kDa exclusion limit, which reliably separates small molecules and salts from the larger valuable proteins.
KNAUER Sepapure columns are made to fit perfectly with AZURA FPLC systems but can also be used with most other FPLC systems on the market.