Proper application of Origen Biomedical stem cell storage products (Part 2)
CAR-T therapy: A solution for cancer treatment
Origen products used in CAR-T therapy
In CAR-T therapy, the collection, storage, and transportation of T cells are crucial steps. Origen Biological provides products that maximize support throughout these stages, including:
- CryoStore™ bags: This is a vital product in the T cell storage process. CryoStore™ bags are designed to withstand extreme cold temperatures, enabling the storage of T cells in liquid nitrogen. These bags protect the cells from damage during freezing and thawing processes.
- CryoPur® DMSO solution: DMSO is a stem cell storage solution that plays a key role in minimizing cell damage during freezing. CryoPur® DMSO is used in combination with CryoStore™ bags to protect T cells during storage and transport.
- O-Wrap™ bags: This product protects CryoStore™ bags during liquid nitrogen storage and transportation. It ensures the safety of the cells throughout the handling process and minimizes the risk of bag rupture.
CAR-T therapy process
The CAR-T therapy process consists of several critical steps, with Origen products supporting each stage to protect T cells and ensure maximum cell quality:
- T Cell collection (Apheresis): After T cells are collected from the patient, they are stored in CryoStore™ bags containing CryoPur® DMSO solution. This process helps protect the cells during transportation from the collection site to the production facility.
- Gene editing and cell cultivation: After collection, T cells undergo gene editing (CAR receptor insertion) in a sterile environment, supported by PermaLife® culture bags. These gas-permeable bags allow for proper gas exchange between the inside and outside, preventing contamination while maintaining cell viability.
- Freezing and storage: Once gene editing is complete, CAR-T cells are packed in CryoStore™ bags and frozen at ultra-low temperatures, preserving cell integrity and efficacy throughout storage.
- CAR-T cell infusion: When the patient is ready for therapy, the CAR-T cells are thawed and infused into the patient’s body. Storing CAR-T cells frozen ensures flexibility for infusion when necessary.
Purpose of CAR-T cell therapy
The purpose of Origen products in CAR-T therapy is to maintain the quality and efficacy of T cells throughout the process, from collection and storage to infusion into the patient’s body. The combination of CryoStore™ bags and CryoPur® DMSO solution ensures cell protection throughout the freezing process, guaranteeing the highest therapeutic efficacy for the patient.
Key Customers
Origen is a preferred supplier in the cell and gene therapy field, including CAR-T. Most pharmaceutical and biotechnology companies developing commercial-scale CAR-T therapies use Origen’s freezing bags and culture bags. For example, the commercial CAR-T product axicabtagene ciloleucel (Yescarta by Kite/Gilead) in clinical trials utilized OriGen’s CS250 freezing bags to package T cell doses for infusion.
Cancer-specialized hospitals, research centers, and biotechnology companies are the primary customers for Origen’s CAR-T products. Organizations such as the U.S. National Cancer Institute (NCI), MD Anderson Cancer Center, and major pharmaceutical companies rely on Origen products for T cell storage during treatment.
Origen also collaborates with the Blood Centers of America (BCA) network – the largest blood supply organization in the U.S. – to provide freezing storage equipment for hematology and advanced therapies. This collaboration has supported over 70 blood centers and therapy organizations nationwide.
Immunology and cell immunotherapy
Origen products for immunology and cell immunotherapy
In the field of immunology, Origen products play a significant role in supporting research and the development of immune cell therapies. Key products include:
- PermaLife® bags: These gas-permeable culture bags are specifically designed for cultivating immune cells like T cells, B cells, and NK cells in a sterile environment. The bags create a stable environment that prevents contamination while ensuring sufficient gas exchange for optimal cell growth. Labs often use PermaLife bags to culture lymphoid (T, B, NK) or dendritic cells in long-term experiments, as they allow for cultures with a larger volume than traditional flasks, reducing contamination risks.
- CryoStore™ bags and CryoPur® DMSO solution: After immune cells are cultured, they are stored in CryoStore™ bags with CryoPur® DMSO solution, which helps maintain cell viability and efficacy for testing or therapy.
- Cell Connect® Kit: This kit includes sterile connectors, filters, and syringes for seamless, sterile transfer between bags/tubes.
These products are designed to be compatible with closed-system operations (isolators or biological safety cabinets with sealed gloves), facilitating GMP-compliant manufacturing of immunotherapy treatments.
Origen product line from culturing to using immune cells
- Culturing immune cells: Immune cells are cultured in PermaLife® bags in a sterile environment to ensure optimal growth and function.
- Storing immune cells: After culturing, cells are transferred into CryoStore™ bags, which are then stored at ultra-low temperatures using CryoPur® DMSO.
- Using immune cells: Immune cells are thawed and used in studies or immune cell therapies. Proper storage minimizes risks when cells are not immediately used.
Purpose of use
Origen products in immunology and cell immunotherapy help scientists and clinicians store, culture, and use immune cells for testing and therapy, supporting the development of advanced treatment methods such as CAR-T, TIL, and NK cell therapies.
Key customers for immune cell therapy products
Origen’s primary customers are research institutes, laboratories, and companies developing immune cell therapies. Many academic institutes and universities use Origen products to conduct research on immune cells.
The U.S. National Cancer Institute (NCI) has used PermaLife® bags to culture expanded T cells from patients in a closed environment for TIL and CAR-T therapies, replacing traditional shaker flasks. This method has allowed them to produce large quantities of active immune cells in sterile conditions, suitable for patient infusion.
Numerous research groups at institutions like the University of Texas MD Anderson, European Institute of Cancer Research (IGR), and the University of Tokyo are also reported to use gas-permeable culture bags (such as Origen or similar) for dendritic cell vaccine trials and NK cell therapy studies.
In the industry, biotechnology companies developing advanced immune therapies (e.g., CAR-NK, TCR-T, or iPSC-based therapies) often choose Origen as a supplier of consumables. Origen is regarded as the “preferred supplier of the industry” for single-use packaging products for cell therapies, and many companies have established long-term supplier relationships with Origen to ensure high-quality materials.
Sartorius Stedim Biotech, a European company specializing in bioreactor systems for cell culture, also distributes Origen bags as part of its integrated solutions for its customers.
For immune labs in China, India, South Korea, and other regions, Origen partners with local distributors to guide product usage, such as instructions in Mandarin for using O-Wrap™ bags to prevent freezing bag rupture.
CAR-T Therapy and Cell Immunotherapy are groundbreaking treatment methods, and the storage of stem cells and immune cells is key to ensuring their success. Origen’s products are highly integrated, with CryoStore™ bags and CryoPur® solutions indispensable in key steps of the process.
Stem cell and immune cell storage can be safely and efficiently done by effectively utilizing Origen’s product ecosystem. If you have any questions, Biogroup Vietnam is always ready to help you choose the right solutions.
Contact us for detailed advice:
- Website: https://biogroupvietnam.com/lien-he
- Email: info@biogroupvietnam.com
