Automating cell isolation with RoboSep™ cell separator
Experienced experts are always looking for automatic cell separation solutions to reduce redundant manual steps. This increases consistency between separations, ensures biosafety, and saves time. The RoboSep™-S & RoboSep™-16 cell separators from Stemcell Technologies were developed to meet these needs. These devices use the EasySep™ magnetic immunoassay technology without the need for separation columns, allowing users to “load the sample and press a button” (walk-away). As a result, the process of isolating various cell types (e.g., T, B, NK immune cells, or even stem cells) becomes simpler and more efficient than ever.
The demand for stable, accurate, and high-performance cell isolation
Cell isolation is a critical step in many research and diagnostic processes (such as immune cell analysis, stem cell isolation, HLA testing, chimerism, etc.). Traditional manual methods (e.g., gradient centrifugation, handheld magnets with magnetic beads, etc.) are often time-consuming and prone to variability between runs. Common challenges include low target cell recovery, insufficient purity, and the risk of errors or contamination from manual handling. A study at Royal Perth Hospital (Australia) showed that automating T and B cell isolation using the RoboSep™ system could double lymphocyte recovery efficiency from the same sample volume compared to manual methods while significantly reducing processing time. Moreover, the automation process reduces errors during sample handling and avoids cross-contamination by using disposable tips. These results emphasize the importance of a stable, automated cell separation system to meet the needs of current research and clinical laboratories.
Key technical features of RoboSep™-S
RoboSep™-S is the second fully automated cell separator generation in the RoboSep™ series. This device has a compact size and comes with an integrated touchscreen, making it easy for users to operate on-site. Below are the key technical features of RoboSep™-S:
Simultaneous processing of 4 samples
RoboSep™-S can process up to 4 samples simultaneously in one run. Each sample can range from 250 µL to 8.5 mL, making it suitable for various sample sources (whole blood, bone marrow fluid, PBMCs, etc.). With the capacity to process four samples in parallel, the device meets the needs of mid-sized sample processing in research and testing labs.
Sequential isolation of multiple cell types
In addition to parallel separation, RoboSep™-S allows sequential isolation of up to 4 different cell types from the same sample (e.g., sequential isolation of T, B, NK, and mono populations from a blood sample). This feature is especially useful for applications that require the isolation of multiple cell populations from a single sample (such as chimerism analysis).
EasySep™ column-free technology
The device uses EasySep™ magnetic beads technology to label and isolate target cells without the need for columns. By not using columns, the isolation process minimizes cell activation and preserves their biological properties. Nano magnetic beads combined with specific antibodies bind to target cells. A magnet then pulls the bead-bound cells out of the mixture, allowing for the collection of a pure and viable cell population for downstream applications.
Intuitive user interface
RoboSep™-S comes with a color touchscreen integrated directly into the device, featuring a user-friendly graphical interface. Users need to select the desired cell isolation protocol (several pre-programmed protocols for different cell types are available), load the sample and reagents into the tray, and press “Run.” Direct handling time is approximately 5 minutes, after which the machine automatically performs the remaining steps. After 25–60 minutes (depending on the protocol type), users can return to collect the purified isolated cells.
Disposable tip system to avoid contamination
All sample aspiration/dispensing and reagent handling are done via disposable pipette tips, eliminating the risk of cross-contamination between samples. Each sample position has its own magnet and separation component, ensuring independence between processing channels. Importantly, RoboSep™-S does not require daily washing or sterilization after use, making maintenance simpler than systems with fixed liquid pathways.
Chemical tracking and data storage
The machine is equipped with a barcode scanner to identify EasySep™ reagents and manage sample information. After each run, the system can generate an End-of-Run report that records operator details, protocol type, reagent lot numbers, etc., facilitating convenient and transparent data retrieval and management.
Thanks to these features, RoboSep™-S is easily placed in a biosafety cabinet or on a lab bench, and multiple devices can be positioned together to increase throughput when needed. This device is particularly suitable for labs that need to isolate cells on a medium scale, requiring flexibility and ease of use.
Key technical features of RoboSep™-16
RoboSep™-16 is a high-capacity version designed to automate large-scale cell isolation processes. It is ideal for testing centers or research institutes with high parallel sample processing needs. Key features of RoboSep™-16 include:
Simultaneous processing of 16 samples
As its name suggests, RoboSep™-16 can process up to 16 samples simultaneously in one run. Each sample can range from approximately 200 µL to 2 mL, making it suitable for smaller sample volumes (e.g., peripheral blood-derived products, enriched PBMC samples, etc.). With 16 sample positions running in parallel, RoboSep™-16 delivers exceptional throughput to meet the high demand in large labs.
Sequential isolation of multiple cell types from multiple samples
The device allows sequential isolation of multiple cell types from different samples simultaneously. Specifically, RoboSep™-16 can sequentially isolate up to 4 types of cells from 4 different samples in one run. This is helpful when multiple samples need to be processed, and each requires the isolation of multiple cell populations. For example, isolating T and B cells from various patients for immune testing.
Software-operated interface
Instead of an integrated touchscreen, RoboSep™-16 comes with a laptop that has installed control software. The intuitive software interface allows users to set up runs similarly to RoboSep™-S, with only about 5 minutes needed to select protocols and load samples. Using a computer control system also enables flexible software updates, data storage on the system, and easy data import/export.
Smart sensor integration
RoboSep™-16 is equipped with electrically conductive tips with sensors that automatically detect the reagent levels of EasySep™ chemicals and buffers during the run. If a reagent bottle is running low, the system will recognize this and automatically switch to a backup bottle without interrupting the separation process. This smart feature ensures smooth operation even when handling large numbers of samples.
Safety and cross-contamination prevention
Similar to RoboSep™-S, the 16 version uses disposable tips and fully automates all sample and reagent handling steps. This reduces technician exposure to biological samples (increasing biosafety) and eliminates the risk of cross-contamination between samples. RoboSep™-16’s robotic pipetting system and automatic magnets ensure that each sample is handled independently, even when running 16 samples simultaneously.
Ideal for large labs
With its high throughput and flexible features, RoboSep™-16 is perfect for HLA testing labs, blood banks, immune research centers, or any facility that needs to isolate a large number of samples daily. The device frees up technicians, who previously spent hours handling samples manually, allowing them to focus on more complex analytical tasks while RoboSep™-16 handles cell separation.
In summary, RoboSep™-16 inherits all the advantages of RoboSep™-S but expands its capabilities to handle larger-scale processing. This device delivers superior performance while maintaining reliability, accuracy, and safety in cell isolation procedures.
Benefits of using RoboSep™-S and RoboSep™-16 cell separators
Using RoboSep™-S and RoboSep™-16 in cell isolation processes brings many practical benefits to laboratories, especially in the fields of immune cell and stem cell isolation. Below are the key benefits of using these systems:
Fully automated – Saves time and labor
Both RoboSep™-S and RoboSep™-16 provide full automation (true walk-away). Technicians only need a few minutes for initial setup and can then focus on other tasks while the machine operates. This significantly saves time and labor compared to manual cell separation, especially when processing large numbers of samples.
High stability and accuracy
Thanks to pre-programmed protocols and the elimination of manual handling, cell isolation results with RoboSep™ are highly reproducible and error-free. Each run strictly follows the correct steps, ensuring accuracy and consistency between isolation batches, which is essential in testing and R&D environments.
Superior recovery efficiency and purity
EasySep™ technology allows for the isolation of target cell populations with high purity and excellent recovery rates. Evaluation reports show that RoboSep™ can double cell recovery efficiency compared to manual methods, even isolating cells from low-quality samples that previous methods could not handle. The isolated cells remain healthy and meet viability and functionality criteria for downstream testing or transplantation.
Versatile applications and flexibility
The RoboSep™ duo meets the needs of isolating most types of cells from various sample sources. You can easily isolate immune cells (T, B, NK, mono, circulating tumor cells, etc.), stem cells (HSC, MSC, etc.), or cells from other tissues and bodily fluids. The option for simultaneous or sequential isolation of multiple cell types provides flexibility for complex research. Additionally, users can customize protocols or use pre-set protocols that suit specific lab needs.
Biosafety and contamination minimization
The closed process and reduced direct contact with samples protect the operator from harmful biological agents. The use of disposable tips and a column-free system also eliminates the risk of cross-contamination between samples, ensuring the integrity of each isolation run.
Simple maintenance and long-lasting performance
RoboSep™-S and RoboSep™-16 are designed to minimize maintenance requirements. Since the system does not use fixed liquid pathways, there is no need for daily washing or sterilization. The device’s mechanical and electronic components are durable, with a self-diagnostic error detection system. Users only need to perform periodic calibration and replace consumables (tips, reagents) as recommended, reducing long-term operational costs.
Due to these benefits, many research laboratories and institutes have chosen RoboSep™-S and RoboSep™-16 as essential tools for automated cell isolation. From clinical applications like HLA testing, immune screening, to cancer research and cell therapy studies, these devices efficiently support and enhance work performance.
Technical support from Biogroup Vietnam
In addition to quality products from STEMCELL Technologies, customers can enjoy comprehensive technical support when choosing RoboSep™-S and RoboSep™-16 at Biogroup Vietnam. As an authorized distributor of laboratory equipment, Biogroup is committed to assisting your lab throughout the implementation and use of the product. Specific support includes:
Expert consultation and device demonstration
Biogroup’s team of experts is ready to provide detailed advice on the cell separation solutions that best suit your needs. Customers can request on-site demos to experience RoboSep™’s effectiveness before investing.
Installation, training, and usage guidance
When purchasing a RoboSep™ device, Biogroup will assist with on-site installation and full technology transfer. Your technicians will be thoroughly trained on device operation, safety protocols, and basic maintenance, ensuring they can use the system proficiently as quickly as possible.
Warranty and periodic maintenance
STEMCELL Technologies’ official warranty standards cover the product. Biogroup offers periodic maintenance and calibration checks to ensure the device operates smoothly. Any technical issues will be addressed quickly by Biogroup’s engineering team, minimizing lab downtime.
Supplies and accessories support
Biogroup ensures the supply of all essential consumables (such as EasySep™ kits, filters, tips, RoboSep™ buffers, etc.) throughout the usage period. You will always have access to reliable supplies to maintain continuous machine operation without interruptions due to missing accessories.
With the customer at the center, Biogroup Vietnam provides dedicated after-sales service, ensuring that customers can fully maximize the benefits of the RoboSep™ system. Timely and professional support from Biogroup guarantees your success in implementing this advanced cell separation system.
Refer to the detailed RoboSep™ cell separator
Refer to detailed RoboSep™ cell separation machines
- Website: https://biogroupvietnam.com/public/lien-he
- Hotline: +84 963 621 421
- Email: info@biogroupvietnam.vn
