Real-time PCR for PCV-2 Detection

Bioassays

Real-time PCR for PCV-2 Detection

Manufacturer:

Status: New

Origin: Spain


The PCV-2 dtec-qPCR Test comprises a series of specific targeted reagents designed for the detection of Porcine circovirus type 2 that relates to the porcine respiratory disease complex (PRDC)the post-weaning multisystemic wasting syndrome (PMWS)the porcine dermatitis and nephropathy syndrome (PDNS), the enteric diseases, and the reproductive problems by using real-time PCR.

Advantages of product:

  1. All GPS kits are manufactured by Genetics PCR Solutions – Spain, be complaint with ISO 9001, ISO 13485 standards.
  2. They are lyophilized, easy to transport and store.
  3. The kit is stable at room temperature (25-30oC) for transportation. The kits should be stored at -20°C ± 2oC upon arrival.
  4. After dissolved, the kits should be divided into small tubes to avoid repeated thawing.
  5. GPSTM-mix, a new high-performance polymerase mastermix, has benefits:
  • Increased sensitivity and efficiency of qPCR reactions.
  • Minimized thermal protocol (Fast-Cycling) allowing a full qPCR run in less tan 45 minutes.
  • High resistance to PCR inhibitors.
  • Compatible with all the open qPCR thermal cyclers on the market.

Kit content:

1) F100 Format (100 reactions):

  • TargetSpecies dtec-qPCR-mix (a lyophilized mixture)
  • Resuspension buffer (130 ul)
  • Dnase/Rnase free water (1.5 ml)
  • GPSTM-mix 4X (500 ul)
  • Standard template (dehydrated target copies for positive control)
  • Template buffer (150 ul)
  • Optional: Internal Control qPCR-mix.

2) Monodose Format (24 or 96 reactions):

  • TargetSpecies MONODOSE dtec-qPCR (a lyophilized mixture)
  • Dnase/Rnase free water (1.5 ml)
  • Standard template (dehydrated target copies for positive control)
  • Template buffer (150 ul)
  • Option: Internal Control qPCR-mix.

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