{"id":11474,"date":"2026-08-08T07:19:35","date_gmt":"2026-08-08T00:19:35","guid":{"rendered":"https:\/\/biogroupvietnam.com\/?p=11474"},"modified":"2026-08-14T07:26:53","modified_gmt":"2026-08-14T00:26:53","slug":"10-hieu-lam-pho-bien-ve-luu-tru-lanh-sau","status":"publish","type":"post","link":"https:\/\/biogroupvietnam.com\/en\/blog\/10-hieu-lam-pho-bien-ve-luu-tru-lanh-sau\/","title":{"rendered":"10 hi\u1ec3u l\u1ea7m ph\u1ed5 bi\u1ebfn v\u1ec1 b\u1ea3o qu\u1ea3n l\u1ea1nh s\u00e2u"},"content":{"rendered":"\n<p>B\u1ea3o qu\u1ea3n l\u1ea1nh s\u00e2u \u2013 Cryogenic Storage \u2013 l\u00e0 n\u1ec1n t\u1ea3ng quan tr\u1ecdng trong Cell Therapy, Biobank, IVF v\u00e0 ng\u00e2n h\u00e0ng m\u00e1u cu\u1ed1ng r\u1ed1n, c\u0169ng nh\u01b0 trong nghi\u00ean c\u1ee9u khoa h\u1ecdc s\u1ef1 s\u1ed1ng. Tuy nhi\u00ean, \u0111\u00e2y c\u0169ng l\u00e0 l\u0129nh v\u1ef1c t\u1ed3n t\u1ea1i nhi\u1ec1u c\u00e1ch hi\u1ec3u \u0111\u01a1n gi\u1ea3n h\u00f3a. M\u1ed9t s\u1ed1 quan ni\u1ec7m th\u01b0\u1eddng g\u1eb7p g\u1ed3m:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Ch\u1ec9 c\u1ea7n cho t\u1ebf b\u00e0o v\u00e0o b\u00ecnh nit\u01a1 l\u1ecfng l\u00e0 \u0111\u1ee7.<\/li>\n\n\n\n<li>T\u1ee7 \u0111\u00f4ng -80\u00b0C c\u00f3 th\u1ec3 thay th\u1ebf b\u00ecnh Cryogenic.<\/li>\n\n\n\n<li>C\u00e0ng l\u1ea1nh th\u00ec m\u1eabu c\u00e0ng t\u1ed1t.<\/li>\n\n\n\n<li>B\u00ecnh nit\u01a1 l\u1ecfng kh\u00f4ng d\u00f9ng \u0111i\u1ec7n n\u00ean kh\u00f4ng s\u1ee3 m\u1ea5t \u0111i\u1ec7n.<\/li>\n\n\n\n<li>Nit\u01a1 l\u1ecfng v\u00f4 tr\u00f9ng n\u00ean kh\u00f4ng th\u1ec3 x\u1ea3y ra nhi\u1ec5m ch\u00e9o.<\/li>\n\n\n\n<li>B\u00ecnh c\u00f2n LN\u2082 ngh\u0129a l\u00e0 m\u1eabu v\u1eabn an to\u00e0n.<\/li>\n\n\n\n<li>Viability cao \u0111\u1ed3ng ngh\u0129a v\u1edbi ch\u1ea5t l\u01b0\u1ee3ng t\u1ebf b\u00e0o t\u1ed1t.<\/li>\n<\/ul>\n\n\n\n<p>Nh\u1eefng nh\u1eadn \u0111\u1ecbnh n\u00e0y c\u00f3 th\u1ec3 ch\u1ee9a m\u1ed9t ph\u1ea7n s\u1ef1 th\u1eadt, nh\u01b0ng n\u1ebfu \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng thi\u1ebfu \u0111i\u1ec1u ki\u1ec7n v\u00e0 \u0111\u00e1nh gi\u00e1 r\u1ee7i ro, ch\u00fang c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn sai s\u00f3t trong thi\u1ebft k\u1ebf, v\u1eadn h\u00e0nh v\u00e0 qu\u1ea3n l\u00fd h\u1ec7 th\u1ed1ng.<\/p>\n\n\n\n<p>B\u00e0i vi\u1ebft d\u01b0\u1edbi \u0111\u00e2y, do \u0111\u1ed9i ng\u0169 Biogroup Vietnam bi\u00ean so\u1ea1n d\u1ef1a tr\u00ean t\u00e0i li\u1ec7u khoa h\u1ecdc \u0111\u01b0\u1ee3c b\u00ecnh duy\u1ec7t v\u00e0 h\u01b0\u1edbng d\u1eabn k\u1ef9 thu\u1eadt qu\u1ed1c t\u1ebf (ISBER, OSHA, PubMed) v\u00e0 kinh nghi\u1ec7m th\u1ef1c t\u1ebf, ph\u00e2n t\u00edch t\u1eebng hi\u1ec3u l\u1ea7m v\u00e0 ch\u1ec9 ra c\u00e1ch ti\u1ebfp c\u1eadn \u0111\u00fang theo qu\u1ea3n l\u00fd r\u1ee7i ro.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"538\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/08\/10-sai-lam-pho-bien-trong-bao-quan-lanh-sau-1200x630-1-1024x538.png\" alt=\"\" class=\"wp-image-11475\" srcset=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/08\/10-sai-lam-pho-bien-trong-bao-quan-lanh-sau-1200x630-1-1024x538.png 1024w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/08\/10-sai-lam-pho-bien-trong-bao-quan-lanh-sau-1200x630-1-300x158.png 300w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/08\/10-sai-lam-pho-bien-trong-bao-quan-lanh-sau-1200x630-1-768x403.png 768w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/08\/10-sai-lam-pho-bien-trong-bao-quan-lanh-sau-1200x630-1-600x315.png 600w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/08\/10-sai-lam-pho-bien-trong-bao-quan-lanh-sau-1200x630-1-90x47.png 90w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/08\/10-sai-lam-pho-bien-trong-bao-quan-lanh-sau-1200x630-1.png 1200w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Myth 1: &#8220;Ch\u1ec9 c\u1ea7n nhi\u1ec7t \u0111\u1ed9 c\u00e0ng th\u1ea5p th\u00ec t\u1ebf b\u00e0o c\u00e0ng \u0111\u01b0\u1ee3c b\u1ea3o v\u1ec7 t\u1ed1t&#8221;<\/h2>\n\n\n\n<p><strong>Fact:<\/strong> Nhi\u1ec7t \u0111\u1ed9 th\u1ea5p r\u1ea5t quan tr\u1ecdng, nh\u01b0ng kh\u00f4ng th\u1ec3 s\u1eeda ch\u1eefa m\u1ed9t quy tr\u00ecnh cryopreservation kh\u00f4ng ph\u00f9 h\u1ee3p.<\/p>\n\n\n\n<p>Cryogenic Storage ch\u1ec9 gi\u00fap duy tr\u00ec tr\u1ea1ng th\u00e1i c\u1ee7a m\u1eabu sau khi t\u1ebf b\u00e0o \u0111\u00e3 \u0111\u01b0\u1ee3c chu\u1ea9n b\u1ecb v\u00e0 \u0111\u00f4ng l\u1ea1nh \u0111\u00fang c\u00e1ch.<\/p>\n\n\n\n<p>N\u1ebfu t\u1ebf b\u00e0o tr\u01b0\u1edbc \u0111\u00f4ng l\u1ea1nh c\u00f3:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Viability th\u1ea5p;<\/li>\n\n\n\n<li>D\u1ea5u hi\u1ec7u stress;<\/li>\n\n\n\n<li>M\u1eadt \u0111\u1ed9 kh\u00f4ng ph\u00f9 h\u1ee3p;<\/li>\n\n\n\n<li>Passage qu\u00e1 cao;<\/li>\n\n\n\n<li>Nhi\u1ec5m mycoplasma;<\/li>\n\n\n\n<li>Ti\u1ebfp x\u00fac v\u1edbi DMSO qu\u00e1 l\u00e2u;<\/li>\n\n\n\n<li>T\u1ed1c \u0111\u1ed9 l\u00e0m l\u1ea1nh kh\u00f4ng ph\u00f9 h\u1ee3p;<\/li>\n<\/ul>\n\n\n\n<p>th\u00ec vi\u1ec7c \u0111\u01b0a m\u1eabu xu\u1ed1ng -150\u00b0C ho\u1eb7c -196\u00b0C kh\u00f4ng th\u1ec3 ph\u1ee5c h\u1ed3i ch\u1ea5t l\u01b0\u1ee3ng \u0111\u00e3 m\u1ea5t.<\/p>\n\n\n\n<p>Cryopreservation l\u00e0 m\u1ed9t quy tr\u00ecnh nhi\u1ec1u b\u01b0\u1edbc, trong \u0111\u00f3 cryoprotectant, t\u1ed1c \u0111\u1ed9 l\u00e0m l\u1ea1nh v\u00e0 t\u1ed1c \u0111\u1ed9 r\u00e3 \u0111\u00f4ng \u0111\u1ec1u \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn s\u1ef1 h\u00ecnh th\u00e0nh b\u0103ng, stress th\u1ea9m th\u1ea5u, t\u1ed5n th\u01b0\u01a1ng m\u00e0ng v\u00e0 kh\u1ea3 n\u0103ng ph\u1ee5c h\u1ed3i c\u1ee7a t\u1ebf b\u00e0o, theo t\u1ed5ng quan c\u1ee7a Whaley v\u00e0 c\u1ed9ng s\u1ef1 tr\u00ean PubMed (<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33757335\/\">Cryopreservation: An Overview of Principles and Cell-Specific Considerations<\/a>).<\/p>\n\n\n\n<p><strong>\u0110i\u1ec1u c\u1ea7n ghi nh\u1edb<\/strong><\/p>\n\n\n\n<p>Kh\u00f4ng n\u00ean h\u1ecfi ri\u00eang: &#8220;B\u00ecnh c\u00f3 \u0111\u1ee7 l\u1ea1nh kh\u00f4ng?&#8221; M\u00e0 ph\u1ea3i \u0111\u00e1nh gi\u00e1 to\u00e0n b\u1ed9 chu\u1ed7i: t\u1ebf b\u00e0o \u0111\u1ea7u v\u00e0o \u2013 m\u00f4i tr\u01b0\u1eddng \u0111\u00f4ng l\u1ea1nh \u2013 t\u1ed1c \u0111\u1ed9 l\u00e0m l\u1ea1nh \u2013 \u0111i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef \u2013 thao t\u00e1c truy xu\u1ea5t \u2013 quy tr\u00ecnh r\u00e3 \u0111\u00f4ng \u2013 QC sau r\u00e3 \u0111\u00f4ng. Xem th\u00eam h\u01b0\u1edbng d\u1eabn l\u1ef1a ch\u1ecdn thi\u1ebft b\u1ecb v\u00e0 ph\u01b0\u01a1ng ph\u00e1p ph\u00f9 h\u1ee3p trong b\u00e0i <a href=\"https:\/\/word-edit.officeapps.live.com\/huong-dan-lua-chon-thiet-bi-cryogenic-storage\">H\u01b0\u1edbng d\u1eabn l\u1ef1a ch\u1ecdn thi\u1ebft b\u1ecb Cryogenic Storage theo lo\u1ea1i m\u1eabu<\/a>.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Myth 2: &#8220;T\u1ee7 \u0111\u00f4ng -80\u00b0C \u0111\u1ee7 \u0111\u1ec3 l\u01b0u tr\u1eef t\u1ebf b\u00e0o l\u00e2u d\u00e0i&#8221;<\/h2>\n\n\n\n<p><strong>Fact:<\/strong> -80\u00b0C th\u01b0\u1eddng ch\u1ec9 ph\u00f9 h\u1ee3p nh\u01b0 m\u1ed9t b\u01b0\u1edbc trung gian ho\u1eb7c l\u01b0u tr\u1eef ng\u1eafn h\u1ea1n \u0111\u1ed1i v\u1edbi nhi\u1ec1u lo\u1ea1i t\u1ebf b\u00e0o s\u1ed1ng.<\/p>\n\n\n\n<p>T\u1ee7 -80\u00b0C r\u1ea5t quan tr\u1ecdng trong ph\u00f2ng th\u00ed nghi\u1ec7m v\u00e0 ph\u00f9 h\u1ee3p v\u1edbi nhi\u1ec1u lo\u1ea1i m\u1eabu nh\u01b0 DNA, RNA, protein, lysate ho\u1eb7c m\u1ed9t s\u1ed1 vi sinh v\u1eadt. Tuy nhi\u00ean, \u0111\u1ed1i v\u1edbi nhi\u1ec1u t\u1ebf b\u00e0o \u0111\u1ed9ng v\u1eadt c\u00f3 v\u00fa, -80\u00b0C th\u01b0\u1eddng kh\u00f4ng \u0111\u01b0\u1ee3c xem l\u00e0 \u0111i\u1ec1u ki\u1ec7n t\u1ed1i \u01b0u cho l\u01b0u tr\u1eef d\u00e0i h\u1ea1n.<\/p>\n\n\n\n<p>C\u00e1c quy tr\u00ecnh ph\u1ed5 bi\u1ebfn th\u01b0\u1eddng l\u00e0m l\u1ea1nh t\u1ebf b\u00e0o trong t\u1ee7 -80\u00b0C ho\u1eb7c thi\u1ebft b\u1ecb controlled-rate freezer, sau \u0111\u00f3 chuy\u1ec3n sang \u0111i\u1ec1u ki\u1ec7n kho\u1ea3ng -135\u00b0C ho\u1eb7c th\u1ea5p h\u01a1n \u0111\u1ec3 l\u01b0u tr\u1eef d\u00e0i h\u1ea1n. H\u01b0\u1edbng d\u1eabn k\u1ef9 thu\u1eadt c\u1ee7a STEMCELL Technologies l\u01b0u \u00fd r\u1eb1ng l\u01b0u t\u1ebf b\u00e0o \u1edf -80\u00b0C d\u01b0\u1edbi m\u1ed9t th\u00e1ng c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c ch\u1ea5p nh\u1eadn trong m\u1ed9t s\u1ed1 quy tr\u00ecnh, nh\u01b0ng th\u1eddi gian n\u00e0y n\u00ean \u0111\u01b0\u1ee3c gi\u1ea3m t\u1ed1i \u0111a \u0111\u1ec3 duy tr\u00ec \u0111\u1ed9 \u1ed5n \u0111\u1ecbnh (theo <a href=\"https:\/\/www.stemcell.com\/cryopreservation-basics-protocols-and-best-practices-for-freezing-cells\">STEMCELL Technologies \u2013 Cryopreservation Basics: Protocols and Best Practices for Freezing Cells<\/a>).<\/p>\n\n\n\n<p>\u1ede -80\u00b0C, m\u1eabu v\u1eabn c\u00f3 th\u1ec3 tr\u1ea3i qua:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>S\u1ef1 t\u00e1i t\u1ed5 ch\u1ee9c c\u1ee7a c\u1ea5u tr\u00fac b\u0103ng;<\/li>\n\n\n\n<li>Chuy\u1ec3n \u0111\u1ed9ng ph\u00e2n t\u1eed \u1edf m\u1ee9c cao h\u01a1n v\u00f9ng cryogenic;<\/li>\n\n\n\n<li>Bi\u1ebfn \u0111\u1ed5i t\u00edch l\u0169y theo th\u1eddi gian;<\/li>\n\n\n\n<li>Nguy c\u01a1 t\u0103ng nhi\u1ec7t nhanh n\u1ebfu t\u1ee7 m\u1ea5t \u0111i\u1ec7n ho\u1eb7c g\u1eb7p l\u1ed7i m\u00e1y n\u00e9n.<\/li>\n<\/ul>\n\n\n\n<p><strong>C\u00e1ch \u00e1p d\u1ee5ng \u0111\u00fang<\/strong><\/p>\n\n\n\n<p>SOP ph\u1ea3i x\u00e1c \u0111\u1ecbnh r\u00f5: -80\u00b0C l\u00e0 \u0111i\u1ec3m trung gian hay \u0111i\u1ec3m l\u01b0u tr\u1eef ch\u00ednh th\u1ee9c; th\u1eddi gian t\u1ed1i \u0111a m\u1eabu \u0111\u01b0\u1ee3c gi\u1eef \u1edf -80\u00b0C; ng\u01b0\u1eddi ch\u1ecbu tr\u00e1ch nhi\u1ec7m chuy\u1ec3n m\u1eabu; \u0111i\u1ec1u ki\u1ec7n v\u00e0 thi\u1ebft b\u1ecb l\u01b0u tr\u1eef \u0111\u00edch; c\u00e1ch ghi nh\u1eadn vi\u1ec7c chuy\u1ec3n m\u1eabu ho\u00e0n t\u1ea5t.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Myth 3: &#8220;M\u1eabu ch\u01b0a r\u00e3 \u0111\u00f4ng th\u00ec dao \u0111\u1ed9ng nhi\u1ec7t kh\u00f4ng g\u00e2y \u1ea3nh h\u01b0\u1edfng&#8221;<\/h2>\n\n\n\n<p><strong>Fact:<\/strong> M\u1eabu kh\u00f4ng c\u1ea7n r\u00e3 \u0111\u00f4ng ho\u00e0n to\u00e0n m\u1edbi c\u00f3 th\u1ec3 b\u1ecb t\u1ed5n th\u01b0\u01a1ng.<\/p>\n\n\n\n<p>Khi nh\u00e2n vi\u00ean n\u00e2ng rack l\u00ean kh\u1ecfi v\u00f9ng l\u01b0u tr\u1eef, m\u1edf n\u1eafp b\u00ecnh qu\u00e1 l\u00e2u ho\u1eb7c chuy\u1ec3n m\u1eabu gi\u1eefa c\u00e1c b\u00ecnh, m\u1eabu c\u00f3 th\u1ec3 tr\u1ea3i qua nh\u1eefng chu k\u1ef3 t\u0103ng r\u1ed3i gi\u1ea3m nhi\u1ec7t.<\/p>\n\n\n\n<p>Nh\u1eefng chu k\u1ef3 n\u00e0y c\u00f3 th\u1ec3 th\u00fac \u0111\u1ea9y: t\u00e1i k\u1ebft tinh; thay \u0111\u1ed5i c\u1ea5u tr\u00fac pha \u0111\u00f4ng l\u1ea1nh; stress l\u00ean m\u00e0ng t\u1ebf b\u00e0o; t\u1ed5n th\u01b0\u01a1ng ty th\u1ec3; gi\u1ea3m kh\u1ea3 n\u0103ng ph\u1ee5c h\u1ed3i sau r\u00e3 \u0111\u00f4ng.<\/p>\n\n\n\n<p>M\u1ed9t nghi\u00ean c\u1ee9u v\u1ec1 t\u1ebf b\u00e0o g\u1ed1c trung m\u00f4 c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb nhau thai cho th\u1ea5y t\u1ef7 l\u1ec7 s\u1ed1ng gi\u1ea3m theo s\u1ed1 chu k\u1ef3 nhi\u1ec7t; \u1ea3nh h\u01b0\u1edfng m\u1ea1nh nh\u1ea5t \u0111\u01b0\u1ee3c quan s\u00e1t khi m\u1eabu dao \u0111\u1ed9ng \u0111\u1ebfn kho\u1ea3ng -80\u00b0C (theo <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28284229\/\">PubMed \u2013 Pogozhykh et al., 2017, &#8220;Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cells&#8221;<\/a>). M\u1ed9t nghi\u00ean c\u1ee9u n\u0103m 2024 tr\u00ean hiPSC c\u0169ng ghi nh\u1eadn nh\u1eefng thay \u0111\u1ed5i li\u00ean quan \u0111\u1ebfn ch\u1ee9c n\u0103ng ty th\u1ec3 sau c\u00e1c chu k\u1ef3 nhi\u1ec7t gi\u1eefa -150\u00b0C v\u00e0 -80\u00b0C, ngay c\u1ea3 khi m\u1eabu v\u1eabn \u1edf tr\u1ea1ng th\u00e1i \u0111\u00f4ng l\u1ea1nh (theo <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39139293\/\">PubMed \u2013 Okuda et al., 2024, &#8220;The impact of repeated temperature cycling on cryopreserved human iPSC viability stems from cytochrome redox state changes&#8221;<\/a>).<\/p>\n\n\n\n<p><strong>\u00dd ngh\u0129a trong v\u1eadn h\u00e0nh<\/strong><\/p>\n\n\n\n<p>\u0110i\u1ec1u quan tr\u1ecdng kh\u00f4ng ch\u1ec9 l\u00e0 m\u1eabu \u0111ang n\u1eb1m trong b\u00ecnh n\u00e0o, m\u00e0 c\u00f2n l\u00e0: m\u1eabu \u0111\u01b0\u1ee3c n\u00e2ng l\u00ean bao nhi\u00eau l\u1ea7n; nhi\u1ec7t \u0111\u1ed9 ti\u1ebfp x\u00fac cao nh\u1ea5t; th\u1eddi gian \u1edf ngo\u00e0i v\u00f9ng l\u01b0u tr\u1eef; t\u1ea7n su\u1ea5t c\u00e1c chu k\u1ef3 nhi\u1ec7t; v\u1ecb tr\u00ed c\u1ee7a m\u1eabu trong rack.<\/p>\n\n\n\n<p>Inventory t\u1ed1t ch\u00ednh l\u00e0 m\u1ed9t bi\u1ec7n ph\u00e1p ki\u1ec3m so\u00e1t nhi\u1ec7t: bi\u1ebft ch\u00ednh x\u00e1c v\u1ecb tr\u00ed gi\u00fap r\u00fat ng\u1eafn th\u1eddi gian m\u1edf b\u00ecnh v\u00e0 tr\u00e1nh thao t\u00e1c tr\u00ean c\u00e1c m\u1eabu kh\u00f4ng li\u00ean quan. Tham kh\u1ea3o th\u00eam <a href=\"https:\/\/word-edit.officeapps.live.com\/quan-ly-inventory-bien-ban-truy-xuat\">Qu\u1ea3n l\u00fd inventory v\u00e0 truy xu\u1ea5t m\u1eabu trong Biobank<\/a>.<\/p>\n\n\n\n<p>[\u1ea2NH MINH H\u1eccA] Rack v\u00e0 cryovial s\u1eafp x\u1ebfp trong b\u00ecnh l\u01b0u tr\u1eef nit\u01a1 l\u1ecfng MVE Cryogenic Storage<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Myth 4: &#8220;Liquid Phase lu\u00f4n b\u1ea3o qu\u1ea3n t\u1ed1t h\u01a1n Vapor Phase v\u00ec l\u1ea1nh h\u01a1n&#8221;<\/h2>\n\n\n\n<p><strong>Fact:<\/strong> C\u1ea3 hai ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ec1u c\u00f3 th\u1ec3 ph\u00f9 h\u1ee3p n\u1ebfu h\u1ec7 th\u1ed1ng \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf, \u0111\u00e1nh gi\u00e1 r\u1ee7i ro v\u00e0 v\u1eadn h\u00e0nh \u0111\u00fang.<\/p>\n\n\n\n<p>Trong Liquid Phase, m\u1eabu \u0111\u01b0\u1ee3c \u0111\u1eb7t tr\u1ef1c ti\u1ebfp trong nit\u01a1 l\u1ecfng, v\u1edbi nhi\u1ec7t \u0111\u1ed9 g\u1ea7n -196\u00b0C. Trong Vapor Phase, m\u1eabu n\u1eb1m ph\u00eda tr\u00ean m\u1eb7t LN\u2082 nh\u01b0ng v\u1eabn \u0111\u01b0\u1ee3c duy tr\u00ec trong v\u00f9ng nhi\u1ec7t \u0111\u1ed9 cryogenic, th\u01b0\u1eddng h\u01b0\u1edbng \u0111\u1ebfn d\u01b0\u1edbi -150\u00b0C ho\u1eb7c theo gi\u1edbi h\u1ea1n \u0111\u00e3 \u0111\u01b0\u1ee3c c\u01a1 s\u1edf x\u00e1c l\u1eadp.<\/p>\n\n\n\n<p>Liquid Phase c\u00f3 l\u1ee3i th\u1ebf v\u1ec1 nhi\u1ec7t \u0111\u1ed9 r\u1ea5t th\u1ea5p v\u00e0 \u0111\u1ed9 \u0111\u1ed3ng \u0111\u1ec1u cao, nh\u01b0ng c\u1ea7n ki\u1ec3m so\u00e1t: t\u00ednh to\u00e0n v\u1eb9n c\u1ee7a v\u1eadt ch\u1ee9a; kh\u1ea3 n\u0103ng LN\u2082 x\u00e2m nh\u1eadp cryovial; nguy c\u01a1 nhi\u1ec5m ch\u00e9o; quy tr\u00ecnh x\u1eed l\u00fd v\u00e0 truy xu\u1ea5t m\u1eabu.<\/p>\n\n\n\n<p>Vapor Phase gi\u00fap gi\u1ea3m ti\u1ebfp x\u00fac tr\u1ef1c ti\u1ebfp gi\u1eefa m\u1eabu v\u00e0 pha l\u1ecfng, v\u00ec v\u1eady th\u01b0\u1eddng \u0111\u01b0\u1ee3c l\u1ef1a ch\u1ecdn nh\u01b0 m\u1ed9t ph\u1ea7n c\u1ee7a chi\u1ebfn l\u01b0\u1ee3c qu\u1ea3n l\u00fd r\u1ee7i ro trong nhi\u1ec1u \u1ee9ng d\u1ee5ng Cell Therapy v\u00e0 Biobank. Tuy nhi\u00ean, \u0111\u1ed9 \u1ed5n \u0111\u1ecbnh c\u1ee7a v\u00f9ng h\u01a1i ph\u1ee5 thu\u1ed9c v\u00e0o thi\u1ebft k\u1ebf b\u00ecnh, m\u1ee9c LN\u2082, v\u1ecb tr\u00ed m\u1eabu v\u00e0 t\u1ea7n su\u1ea5t m\u1edf n\u1eafp. M\u1ed9t t\u1ed5ng quan v\u1ec1 cryopreservation trong cell therapy khuy\u1ebfn ngh\u1ecb n\u00ean tr\u00e1nh l\u01b0u tr\u1eef tr\u1ef1c ti\u1ebfp trong LN\u2082 d\u1ea1ng l\u1ecfng \u1edf c\u00e1c \u1ee9ng d\u1ee5ng l\u00e2m s\u00e0ng do nguy c\u01a1 nhi\u1ec5m vi sinh v\u1eadt th\u1ef1c s\u1ef1 t\u1ed3n t\u1ea1i, v\u00e0 m\u1eabu n\u00ean \u0111\u01b0\u1ee3c l\u01b0u \u1edf v\u00f9ng h\u01a1i ngay ph\u00eda tr\u00ean b\u1ec1 m\u1eb7t LN\u2082 (d\u01b0\u1edbi -150\u00b0C) \u0111\u1ec3 \u0111\u1ea1t \u0111\u1ed9 \u1ed5n \u0111\u1ecbnh t\u1ed1i \u0111a (theo <a href=\"https:\/\/www.frontiersin.org\/journals\/medicine\/articles\/10.3389\/fmed.2020.592242\/full\">Frontiers \u2013 Meneghel, Kilbride &amp; Morris, 2020, &#8220;Cryopreservation as a Key Element in the Successful Delivery of Cell-Based Therapies \u2013 A Review&#8221;<\/a>).<\/p>\n\n\n\n<p><strong>K\u1ebft lu\u1eadn th\u1ef1c t\u1ebf<\/strong><\/p>\n\n\n\n<p>Kh\u00f4ng n\u00ean l\u1ef1a ch\u1ecdn d\u1ef1a tr\u00ean kh\u1ea9u hi\u1ec7u &#8220;Liquid Phase = t\u1ed1t h\u01a1n&#8221; hay &#8220;Vapor Phase = hi\u1ec7n \u0111\u1ea1i h\u01a1n&#8221;. C\u1ea7n l\u1ef1a ch\u1ecdn d\u1ef1a tr\u00ean: lo\u1ea1i m\u1eabu; v\u1eadt ch\u1ee9a; m\u1ee5c \u0111\u00edch s\u1eed d\u1ee5ng; y\u00eau c\u1ea7u ch\u1ea5t l\u01b0\u1ee3ng; \u0111\u00e1nh gi\u00e1 r\u1ee7i ro; hi\u1ec7u su\u1ea5t nhi\u1ec7t c\u1ee7a thi\u1ebft b\u1ecb; kh\u1ea3 n\u0103ng monitoring v\u00e0 b\u1ea3o tr\u00ec.<\/p>\n\n\n\n<p><strong>Xem th\u00eam:<\/strong> <a href=\"https:\/\/word-edit.officeapps.live.com\/vapor-phase-vs-liquid-phase-storage\">So s\u00e1nh Vapor Phase v\u00e0 Liquid Phase Storage cho t\u1eebng \u1ee9ng d\u1ee5ng<\/a>.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Myth 5: &#8220;Nit\u01a1 l\u1ecfng c\u00f3 nhi\u1ec7t \u0111\u1ed9 r\u1ea5t th\u1ea5p n\u00ean t\u1ef1 nhi\u00ean v\u00f4 tr\u00f9ng&#8221;<\/h2>\n\n\n\n<p><strong>Fact:<\/strong> Nhi\u1ec7t \u0111\u1ed9 c\u1ef1c th\u1ea5p kh\u00f4ng \u0111\u1ed3ng ngh\u0129a v\u1edbi v\u00f4 tr\u00f9ng.<\/p>\n\n\n\n<p>Nit\u01a1 l\u1ecfng kh\u00f4ng n\u00ean \u0111\u01b0\u1ee3c m\u1eb7c \u0111\u1ecbnh l\u00e0 m\u00f4i tr\u01b0\u1eddng v\u00f4 tr\u00f9ng. Vi sinh v\u1eadt c\u00f3 th\u1ec3 hi\u1ec7n di\u1ec7n trong b\u1ed3n ch\u1ee9a, h\u1ec7 th\u1ed1ng c\u1ea5p ho\u1eb7c m\u00f4i tr\u01b0\u1eddng li\u00ean quan, v\u00e0 tr\u1ea1ng th\u00e1i l\u1ea1nh s\u00e2u c\u00f3 th\u1ec3 duy tr\u00ec kh\u1ea3 n\u0103ng t\u1ed3n t\u1ea1i c\u1ee7a m\u1ed9t s\u1ed1 t\u00e1c nh\u00e2n thay v\u00ec ti\u00eau di\u1ec7t ch\u00fang.<\/p>\n\n\n\n<p>M\u1ed9t t\u1ed5ng quan v\u1ec1 c\u00e1c b\u1ed3n l\u01b0u tr\u1eef LN\u2082 ghi nh\u1eadn s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a vi sinh v\u1eadt l\u00e0 m\u1ed9t nguy c\u01a1 c\u1ea7n \u0111\u01b0\u1ee3c qu\u1ea3n l\u00fd trong cryostorage d\u00e0i h\u1ea1n (theo <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34559283\/\">PubMed \u2013 Bajerski, Nagel &amp; Overmann, 2021, &#8220;Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?&#8221;<\/a>).<\/p>\n\n\n\n<p>Nguy c\u01a1 nhi\u1ec5m ch\u00e9o t\u0103ng khi: v\u1eadt ch\u1ee9a kh\u00f4ng k\u00edn; cryovial b\u1ecb n\u1ee9t ho\u1eb7c h\u1ecfng; m\u1eabu ti\u1ebfp x\u00fac tr\u1ef1c ti\u1ebfp v\u1edbi LN\u2082; c\u00e1c m\u1eabu c\u00f3 nguy c\u01a1 sinh h\u1ecdc kh\u00e1c nhau \u0111\u01b0\u1ee3c l\u01b0u chung m\u00e0 kh\u00f4ng ph\u00e2n khu; quy tr\u00ecnh v\u1ec7 sinh ho\u1eb7c kh\u1eed nhi\u1ec5m ch\u01b0a ph\u00f9 h\u1ee3p.<\/p>\n\n\n\n<p>Trong l\u0129nh v\u1ef1c IVF, c\u00e1c nghi\u00ean c\u1ee9u th\u1ef1c nghi\u1ec7m c\u0169ng cho th\u1ea5y kh\u1ea3 n\u0103ng trao \u0111\u1ed5i t\u00e1c nh\u00e2n gi\u1eefa LN\u2082 b\u1ecb nhi\u1ec5m v\u00e0 m\u1eabu khi v\u1eadt ch\u1ee9a kh\u00f4ng k\u00edn ho\u00e0n to\u00e0n (theo <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28890894\/\">PubMed \u2013 Joaquim et al., 2017, &#8220;Risk of Contamination of Gametes and Embryos during Cryopreservation and Measures to Prevent Cross-Contamination&#8221;<\/a>).<\/p>\n\n\n\n<p><strong>Bi\u1ec7n ph\u00e1p ki\u1ec3m so\u00e1t<\/strong><\/p>\n\n\n\n<p>S\u1eed d\u1ee5ng v\u1eadt ch\u1ee9a ph\u00f9 h\u1ee3p v\u1edbi \u0111i\u1ec1u ki\u1ec7n cryogenic; ki\u1ec3m so\u00e1t t\u00ednh to\u00e0n v\u1eb9n c\u1ee7a h\u1ec7 th\u1ed1ng \u0111\u00f3ng g\u00f3i; ph\u00e2n khu ho\u1eb7c c\u00e1ch ly m\u1eabu c\u00f3 nguy c\u01a1; \u0111\u00e1nh gi\u00e1 l\u1ef1a ch\u1ecdn Vapor Phase; c\u00f3 quy tr\u00ecnh v\u1ec7 sinh v\u00e0 kh\u1eed nhi\u1ec5m; qu\u1ea3n l\u00fd ngu\u1ed3n v\u00e0 \u0111\u01b0\u1eddng c\u1ea5p LN\u2082; th\u1ef1c hi\u1ec7n \u0111\u00e1nh gi\u00e1 r\u1ee7i ro theo t\u1eebng \u1ee9ng d\u1ee5ng.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Myth 6: &#8220;B\u00ecnh c\u00f2n nit\u01a1 l\u1ecfng ngh\u0129a l\u00e0 t\u1ea5t c\u1ea3 m\u1eabu \u0111\u1ec1u an to\u00e0n&#8221;<\/h2>\n\n\n\n<p><strong>Fact:<\/strong> M\u1ee9c LN\u2082 ch\u1ec9 l\u00e0 m\u1ed9t trong nhi\u1ec1u ch\u1ec9 s\u1ed1 c\u1ee7a tr\u1ea1ng th\u00e1i ki\u1ec3m so\u00e1t.<\/p>\n\n\n\n<p>M\u1ed9t b\u00ecnh v\u1eabn c\u00f2n LN\u2082 nh\u01b0ng v\u1eabn c\u00f3 th\u1ec3 t\u1ed3n t\u1ea1i c\u00e1c v\u1ea5n \u0111\u1ec1 nh\u01b0: nhi\u1ec7t \u0111\u1ed9 v\u00f9ng tr\u00ean cao h\u01a1n gi\u1edbi h\u1ea1n; c\u1ea3m bi\u1ebfn \u0111\u1eb7t kh\u00f4ng \u0111\u1ea1i di\u1ec7n cho v\u1ecb tr\u00ed m\u1eabu; controller ho\u1eb7c alarm b\u1ecb l\u1ed7i; s\u1ef1 ph\u00e2n b\u1ed1 nhi\u1ec7t kh\u00f4ng \u0111\u1ed3ng \u0111\u1ec1u; Auto Fill ho\u1ea1t \u0111\u1ed9ng kh\u00f4ng \u1ed5n \u0111\u1ecbnh; ch\u00e2n kh\u00f4ng suy gi\u1ea3m; m\u1eabu b\u1ecb \u0111\u1eb7t sai v\u1ecb tr\u00ed; rack th\u01b0\u1eddng xuy\u00ean b\u1ecb n\u00e2ng l\u00ean.<\/p>\n\n\n\n<p>\u0110\u1eb7c bi\u1ec7t trong Vapor Phase, vi\u1ec7c c\u00f2n LN\u2082 \u1edf \u0111\u00e1y b\u00ecnh kh\u00f4ng t\u1ef1 \u0111\u1ed9ng ch\u1ee9ng minh r\u1eb1ng m\u1ecdi m\u1eabu t\u1ea1i m\u1ecdi v\u1ecb tr\u00ed \u0111\u1ec1u \u0111\u01b0\u1ee3c duy tr\u00ec \u1edf c\u00f9ng m\u1ed9t nhi\u1ec7t \u0111\u1ed9.<\/p>\n\n\n\n<p><strong>H\u1ec7 th\u1ed1ng c\u1ea7n theo d\u00f5i \u0111\u1ed3ng th\u1eddi<\/strong><\/p>\n\n\n\n<p>Nhi\u1ec7t \u0111\u1ed9 t\u1ea1i v\u00f9ng m\u1eabu; m\u1ee9c LN\u2082; t\u1ea7n su\u1ea5t Auto Fill; th\u1eddi gian ph\u1ee5c h\u1ed3i sau m\u1edf n\u1eafp; l\u1ecbch s\u1eed alarm; xu h\u01b0\u1edbng ti\u00eau hao LN\u2082; tr\u1ea1ng th\u00e1i ngu\u1ed3n \u0111i\u1ec7n; t\u00ecnh tr\u1ea1ng c\u1ea3m bi\u1ebfn; c\u00e1c thao t\u00e1c can thi\u1ec7p.<\/p>\n\n\n\n<p>ISBER xem monitoring thi\u1ebft b\u1ecb, m\u00f4i tr\u01b0\u1eddng l\u01b0u tr\u1eef, k\u1ebf ho\u1ea1ch d\u1ef1 ph\u00f2ng v\u00e0 qu\u1ea3n l\u00fd thay th\u1ebf thi\u1ebft b\u1ecb l\u00e0 nh\u1eefng th\u00e0nh ph\u1ea7n quan tr\u1ecdng trong qu\u1ea3n tr\u1ecb biobank (theo <a href=\"https:\/\/www.isber.org\/page\/BPR\">ISBER \u2013 Best Practices for Repositories<\/a>).<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Myth 7: &#8220;B\u00ecnh nit\u01a1 l\u1ecfng kh\u00f4ng d\u00f9ng \u0111i\u1ec7n n\u00ean m\u1ea5t \u0111i\u1ec7n kh\u00f4ng \u1ea3nh h\u01b0\u1edfng&#8221;<\/h2>\n\n\n\n<p><strong>Fact:<\/strong> Kh\u1ea3 n\u0103ng l\u00e0m l\u1ea1nh c\u00f3 th\u1ec3 ti\u1ebfp t\u1ee5c, nh\u01b0ng nhi\u1ec1u l\u1edbp b\u1ea3o v\u1ec7 kh\u00e1c v\u1eabn ph\u1ee5 thu\u1ed9c v\u00e0o \u0111i\u1ec7n.<\/p>\n\n\n\n<p>M\u1ed9t b\u00ecnh c\u00e1ch nhi\u1ec7t t\u1ed1t c\u00f3 th\u1ec3 ti\u1ebfp t\u1ee5c duy tr\u00ec \u0111i\u1ec1u ki\u1ec7n l\u1ea1nh nh\u1edd l\u01b0\u1ee3ng LN\u2082 hi\u1ec7n c\u00f3, ngay c\u1ea3 khi ngu\u1ed3n \u0111i\u1ec7n b\u1ecb gi\u00e1n \u0111o\u1ea1n. \u0110\u00e2y l\u00e0 m\u1ed9t \u01b0u \u0111i\u1ec3m l\u1edbn so v\u1edbi t\u1ee7 \u0111\u00f4ng c\u01a1 h\u1ecdc.<\/p>\n\n\n\n<p>Tuy nhi\u00ean, c\u00e1c h\u1ec7 th\u1ed1ng sau c\u00f3 th\u1ec3 m\u1ea5t ch\u1ee9c n\u0103ng: controller; Auto Fill; temperature monitoring; level monitoring; alarm t\u1eeb xa; h\u1ec7 th\u1ed1ng l\u01b0u d\u1eef li\u1ec7u; m\u1ea1ng internet ho\u1eb7c k\u1ebft n\u1ed1i cloud; h\u1ec7 th\u1ed1ng th\u00f4ng gi\u00f3; oxygen monitoring; BMS ho\u1eb7c SCADA.<\/p>\n\n\n\n<p>N\u1ebfu m\u1ea5t \u0111i\u1ec7n k\u00e9o d\u00e0i v\u00e0 Auto Fill kh\u00f4ng ho\u1ea1t \u0111\u1ed9ng, ngu\u1ed3n LN\u2082 trong b\u00ecnh s\u1ebd ti\u1ebfp t\u1ee5c gi\u1ea3m. N\u1ebfu kh\u00f4ng c\u00f3 c\u1ea3nh b\u00e1o ho\u1eb7c nh\u00e2n vi\u00ean kh\u00f4ng nh\u1eadn \u0111\u01b0\u1ee3c th\u00f4ng tin, r\u1ee7i ro c\u00f3 th\u1ec3 t\u0103ng m\u00e0 kh\u00f4ng \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n.<\/p>\n\n\n\n<p><strong>K\u1ebf ho\u1ea1ch d\u1ef1 ph\u00f2ng c\u1ea7n bao g\u1ed3m<\/strong><\/p>\n\n\n\n<p>UPS cho controller v\u00e0 monitoring; ngu\u1ed3n \u0111i\u1ec7n d\u1ef1 ph\u00f2ng; c\u1ea3nh b\u00e1o m\u1ea5t \u0111i\u1ec7n; ki\u1ec3m tra th\u1ee7 c\u00f4ng khi h\u1ec7 th\u1ed1ng m\u1ea5t k\u1ebft n\u1ed1i; ngu\u1ed3n cung LN\u2082 d\u1ef1 ph\u00f2ng; ng\u01b0\u1eddi tr\u1ef1c ngo\u00e0i gi\u1edd; b\u00ecnh c\u00f3 dung l\u01b0\u1ee3ng tr\u1ed1ng; k\u1ecbch b\u1ea3n chuy\u1ec3n m\u1eabu kh\u1ea9n c\u1ea5p.<\/p>\n\n\n\n<p><strong>Xem th\u00eam:<\/strong> <a href=\"https:\/\/word-edit.officeapps.live.com\/ke-hoach-du-phong-chuyen-mau-khan-cap\">T\u01b0 v\u1ea5n k\u1ebf ho\u1ea1ch d\u1ef1 ph\u00f2ng v\u00e0 chuy\u1ec3n m\u1eabu kh\u1ea9n c\u1ea5p<\/a>.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Myth 8: &#8220;Ch\u1ec9 c\u1ea7n c\u00f3 alarm l\u00e0 h\u1ec7 th\u1ed1ng \u0111\u00e3 an to\u00e0n&#8221;<\/h2>\n\n\n\n<p><strong>Fact:<\/strong> Alarm kh\u00f4ng c\u00f3 gi\u00e1 tr\u1ecb n\u1ebfu kh\u00f4ng c\u00f3 ng\u01b0\u1eddi nh\u1eadn, ph\u1ea3n \u1ee9ng v\u00e0 escalation.<\/p>\n\n\n\n<p>Nhi\u1ec1u c\u01a1 s\u1edf \u0111\u00e3 trang b\u1ecb c\u1ea3m bi\u1ebfn v\u00e0 ph\u1ea7n m\u1ec1m nh\u01b0ng v\u1eabn ch\u01b0a x\u00e2y d\u1ef1ng m\u1ed9t ch\u01b0\u01a1ng tr\u00ecnh Alarm Management ho\u00e0n ch\u1ec9nh.<\/p>\n\n\n\n<p>C\u00e1c t\u00ecnh hu\u1ed1ng th\u01b0\u1eddng g\u1eb7p g\u1ed3m: danh s\u00e1ch ng\u01b0\u1eddi nh\u1eadn kh\u00f4ng \u0111\u01b0\u1ee3c c\u1eadp nh\u1eadt; tin nh\u1eafn ho\u1eb7c email kh\u00f4ng g\u1eedi \u0111\u01b0\u1ee3c; kh\u00f4ng c\u00f3 ng\u01b0\u1eddi tr\u1ef1c ngo\u00e0i gi\u1edd; nh\u00e2n vi\u00ean t\u1eaft alarm nh\u01b0ng kh\u00f4ng \u0111i\u1ec1u tra nguy\u00ean nh\u00e2n; kh\u00f4ng quy \u0111\u1ecbnh th\u1eddi gian ph\u1ea3n h\u1ed3i; kh\u00f4ng c\u00f3 ng\u01b0\u1eddi thay th\u1ebf khi ng\u01b0\u1eddi ph\u1ee5 tr\u00e1ch v\u1eafng m\u1eb7t; kh\u00f4ng th\u1eed alarm \u0111\u1ecbnh k\u1ef3; kh\u00f4ng m\u1edf deviation ho\u1eb7c CAPA khi s\u1ef1 ki\u1ec7n l\u1eb7p l\u1ea1i.<\/p>\n\n\n\n<p><strong>M\u1ed9t alarm management hi\u1ec7u qu\u1ea3 ph\u1ea3i tr\u1ea3 l\u1eddi \u0111\u01b0\u1ee3c<\/strong><\/p>\n\n\n\n<p>Th\u00f4ng s\u1ed1 n\u00e0o \u0111ang \u0111\u01b0\u1ee3c gi\u00e1m s\u00e1t? Gi\u1edbi h\u1ea1n c\u1ea3nh b\u00e1o d\u1ef1a tr\u00ean c\u01a1 s\u1edf n\u00e0o? Ai l\u00e0 ng\u01b0\u1eddi nh\u1eadn \u0111\u1ea7u ti\u00ean? Ph\u1ea3i x\u00e1c nh\u1eadn trong bao l\u00e2u? Khi n\u00e0o c\u1ea7n chuy\u1ec3n c\u1ea5p? Ai c\u00f3 quy\u1ec1n ra quy\u1ebft \u0111\u1ecbnh chuy\u1ec3n m\u1eabu? H\u00e0nh \u0111\u1ed9ng v\u00e0 d\u1eef li\u1ec7u ph\u1ea3i \u0111\u01b0\u1ee3c ghi nh\u1eadn th\u1ebf n\u00e0o? Khi n\u00e0o s\u1ef1 ki\u1ec7n \u0111\u01b0\u1ee3c xem l\u00e0 \u0111\u00f3ng?<\/p>\n\n\n\n<p>C\u00f3 c\u1ea3m bi\u1ebfn l\u00e0 monitoring. C\u00f3 ng\u01b0\u1eddi, tr\u00e1ch nhi\u1ec7m, th\u1eddi gian ph\u1ea3n h\u1ed3i v\u00e0 quy tr\u00ecnh \u0111i\u1ec1u tra m\u1edbi l\u00e0 qu\u1ea3n l\u00fd r\u1ee7i ro.<\/p>\n\n\n\n<p><strong>Tham kh\u1ea3o:<\/strong> <a href=\"https:\/\/word-edit.officeapps.live.com\/thiet-ke-alarm-escalation\">Thi\u1ebft k\u1ebf alarm escalation cho h\u1ec7 th\u1ed1ng b\u1ea3o qu\u1ea3n l\u1ea1nh s\u00e2u<\/a>.<\/p>\n\n\n\n<p>[\u1ea2NH MINH H\u1eccA] S\u01a1 \u0111\u1ed3 quy tr\u00ecnh escalation c\u1ea3nh b\u00e1o alarm management trong h\u1ec7 th\u1ed1ng Cryogenic Storage<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Myth 9: &#8220;Viability cao sau r\u00e3 \u0111\u00f4ng ngh\u0129a l\u00e0 t\u1ebf b\u00e0o v\u1eabn \u0111\u1ea1t ch\u1ea5t l\u01b0\u1ee3ng&#8221;<\/h2>\n\n\n\n<p><strong>Fact:<\/strong> Viability ch\u1ec9 l\u00e0 m\u1ed9t ph\u1ea7n c\u1ee7a ch\u1ea5t l\u01b0\u1ee3ng t\u1ebf b\u00e0o.<\/p>\n\n\n\n<p>Viability th\u01b0\u1eddng ph\u1ea3n \u00e1nh t\u1ef7 l\u1ec7 t\u1ebf b\u00e0o c\u00f2n nguy\u00ean m\u00e0ng ho\u1eb7c kh\u00f4ng b\u1eaft m\u00e0u v\u1edbi m\u1ed9t thu\u1ed1c nhu\u1ed9m nh\u1ea5t \u0111\u1ecbnh t\u1ea1i th\u1eddi \u0111i\u1ec3m ki\u1ec3m tra.<\/p>\n\n\n\n<p>Tuy nhi\u00ean, m\u1ed9t t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c ph\u00e2n lo\u1ea1i l\u00e0 &#8220;s\u1ed1ng&#8221; ch\u01b0a ch\u1eafc: ph\u1ee5c h\u1ed3i t\u1ed1t sau nu\u00f4i c\u1ea5y; b\u00e1m d\u00ednh b\u00ecnh th\u01b0\u1eddng; t\u0103ng sinh nh\u01b0 tr\u01b0\u1edbc; duy tr\u00ec ki\u1ec3u h\u00ecnh; c\u00f3 ch\u1ee9c n\u0103ng mi\u1ec5n d\u1ecbch; c\u00f3 kh\u1ea3 n\u0103ng bi\u1ec7t h\u00f3a; \u0111\u1ea1t potency; duy tr\u00ec ho\u1ea1t t\u00ednh ty th\u1ec3; \u0111\u00e1p \u1ee9ng m\u1ee5c \u0111\u00edch s\u1eed d\u1ee5ng l\u00e2m s\u00e0ng.<\/p>\n\n\n\n<p>C\u00e1c t\u00e0i li\u1ec7u v\u1ec1 cryopreservation nh\u1ea5n m\u1ea1nh r\u1eb1ng ch\u1ea5t l\u01b0\u1ee3ng sau r\u00e3 \u0111\u00f4ng n\u00ean \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 b\u1eb1ng nhi\u1ec1u ch\u1ec9 s\u1ed1, kh\u00f4ng ch\u1ec9 m\u1ed9t ph\u00e9p \u0111o viability. Nh\u1eefng \u1ea3nh h\u01b0\u1edfng c\u1ee7a qu\u00e1 tr\u00ecnh \u0111\u00f4ng l\u1ea1nh c\u00f3 th\u1ec3 bi\u1ec3u hi\u1ec7n \u1edf recovery, ch\u1ee9c n\u0103ng t\u1ebf b\u00e0o v\u00e0 t\u00ednh nh\u1ea5t qu\u00e1n c\u1ee7a s\u1ea3n ph\u1ea9m (theo <a href=\"https:\/\/www.frontiersin.org\/journals\/medicine\/articles\/10.3389\/fmed.2020.592242\/full\">Frontiers \u2013 Meneghel &amp; Kilbride, &#8220;Cryopreservation as a Key Element in the Successful Delivery of Cell-Based Therapies \u2013 A Review&#8221;<\/a>).<\/p>\n\n\n\n<p><strong>\u0110\u00e1nh gi\u00e1 ph\u00f9 h\u1ee3p c\u00f3 th\u1ec3 bao g\u1ed3m<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>Nh\u00f3m ch\u1ec9 ti\u00eau<\/strong><\/td><td><strong>V\u00ed d\u1ee5<\/strong><\/td><\/tr><tr><td>S\u1ed1 l\u01b0\u1ee3ng<\/td><td>Total cell recovery, viable cell recovery<\/td><\/tr><tr><td>\u0110\u1ed9 s\u1ed1ng<\/td><td>Viability ngay sau thaw v\u00e0 sau ph\u1ee5c h\u1ed3i<\/td><\/tr><tr><td>T\u0103ng sinh<\/td><td>Doubling time, expansion capacity<\/td><\/tr><tr><td>H\u00ecnh th\u00e1i<\/td><td>K\u00edch th\u01b0\u1edbc, \u0111\u1ed9 b\u00e1m d\u00ednh, c\u1ea5u tr\u00fac<\/td><\/tr><tr><td>Ki\u1ec3u h\u00ecnh<\/td><td>Marker b\u1eb1ng flow cytometry<\/td><\/tr><tr><td>Ch\u1ee9c n\u0103ng<\/td><td>Potency, cytotoxicity, differentiation<\/td><\/tr><tr><td>Chuy\u1ec3n h\u00f3a<\/td><td>Mitochondrial activity<\/td><\/tr><tr><td>Di truy\u1ec1n<\/td><td>Karyotype ho\u1eb7c genomic stability<\/td><\/tr><tr><td>An to\u00e0n<\/td><td>Sterility, mycoplasma, endotoxin<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>\u0110\u1ed1i v\u1edbi Cell Therapy, ti\u00eau ch\u00ed cu\u1ed1i c\u00f9ng ph\u1ea3i g\u1eafn v\u1edbi intended use c\u1ee7a s\u1ea3n ph\u1ea9m, kh\u00f4ng ch\u1ec9 v\u1edbi m\u1ed9t gi\u00e1 tr\u1ecb viability \u0111\u1eb9p.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Myth 10: &#8220;B\u00ecnh Cryogenic g\u1ea7n nh\u01b0 kh\u00f4ng c\u00f3 b\u1ed9 ph\u1eadn chuy\u1ec3n \u0111\u1ed9ng n\u00ean kh\u00f4ng c\u1ea7n b\u1ea3o tr\u00ec&#8221;<\/h2>\n\n\n\n<p><strong>Fact:<\/strong> To\u00e0n b\u1ed9 h\u1ec7 th\u1ed1ng Cryogenic Storage v\u1eabn c\u1ea7n preventive maintenance v\u00e0 theo d\u00f5i xu h\u01b0\u1edbng.<\/p>\n\n\n\n<p>B\u1ea3n th\u00e2n v\u1ecf b\u00ecnh c\u00f3 th\u1ec3 \u00edt b\u1ed9 ph\u1eadn chuy\u1ec3n \u0111\u1ed9ng, nh\u01b0ng h\u1ec7 th\u1ed1ng th\u01b0\u1eddng c\u00f2n g\u1ed3m: c\u1ea3m bi\u1ebfn nhi\u1ec7t \u0111\u1ed9; c\u1ea3m bi\u1ebfn m\u1ee9c; van Auto Fill; controller; \u0111\u01b0\u1eddng \u1ed1ng c\u1ea5p LN\u2082; k\u1ebft n\u1ed1i ch\u00e2n kh\u00f4ng; alarm; pin ho\u1eb7c UPS; oxygen monitor; ph\u1ea7n m\u1ec1m v\u00e0 h\u1ec7 th\u1ed1ng d\u1eef li\u1ec7u.<\/p>\n\n\n\n<p>Ngo\u00e0i ra, hi\u1ec7u qu\u1ea3 c\u00e1ch nhi\u1ec7t ch\u00e2n kh\u00f4ng c\u00f3 th\u1ec3 suy gi\u1ea3m theo th\u1eddi gian ho\u1eb7c do h\u01b0 h\u1ecfng.<\/p>\n\n\n\n<p><strong>C\u00e1c d\u1ea5u hi\u1ec7u c\u1ea7n \u0111\u01b0\u1ee3c xem x\u00e9t g\u1ed3m<\/strong><\/p>\n\n\n\n<p>Ti\u00eau hao LN\u2082 t\u0103ng; t\u1ea7n su\u1ea5t Auto Fill t\u0103ng; ng\u01b0ng t\u1ee5 ho\u1eb7c \u0111\u00f3ng b\u0103ng b\u1ea5t th\u01b0\u1eddng tr\u00ean v\u1ecf; alarm m\u1ee9c th\u1ea5p xu\u1ea5t hi\u1ec7n th\u01b0\u1eddng xuy\u00ean; th\u1eddi gian ph\u1ee5c h\u1ed3i nhi\u1ec7t k\u00e9o d\u00e0i; sai l\u1ec7ch gi\u1eefa c\u1ea3m bi\u1ebfn v\u00e0 ki\u1ec3m tra \u0111\u1ed9c l\u1eadp.<\/p>\n\n\n\n<p><strong>B\u1ea3o tr\u00ec d\u1ef1 ph\u00f2ng n\u00ean bao g\u1ed3m<\/strong><\/p>\n\n\n\n<p>Ki\u1ec3m tra ngo\u1ea1i quan; review xu h\u01b0\u1edbng ti\u00eau hao LN\u2082; th\u1eed alarm; hi\u1ec7u chu\u1ea9n c\u1ea3m bi\u1ebfn; ki\u1ec3m tra van v\u00e0 controller; ki\u1ec3m tra ngu\u1ed3n d\u1ef1 ph\u00f2ng; ki\u1ec3m tra \u0111\u01b0\u1eddng c\u1ea5p v\u00e0 k\u1ebft n\u1ed1i; review d\u1eef li\u1ec7u b\u1ea5t th\u01b0\u1eddng; x\u00e1c nh\u1eadn dung l\u01b0\u1ee3ng d\u1ef1 ph\u00f2ng; di\u1ec5n t\u1eadp chuy\u1ec3n m\u1eabu.<\/p>\n\n\n\n<p>M\u1ed9t h\u1ec7 th\u1ed1ng ch\u1ec9 \u0111\u01b0\u1ee3c s\u1eeda khi \u0111\u00e3 h\u1ecfng l\u00e0 m\u00f4 h\u00ecnh reactive maintenance. \u0110\u1ed1i v\u1edbi c\u00e1c m\u1eabu kh\u00f4ng th\u1ec3 thay th\u1ebf, c\u00e1ch ti\u1ebfp c\u1eadn n\u00e0y kh\u00f4ng \u0111\u1ee7 an to\u00e0n.<\/p>\n\n\n\n<p><strong>Xem th\u00eam:<\/strong> <a href=\"https:\/\/word-edit.officeapps.live.com\/dich-vu-preventive-maintenance\">D\u1ecbch v\u1ee5 Preventive Maintenance cho h\u1ec7 th\u1ed1ng Cryogenic Storage<\/a>.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Ba_hieu_lam_bo_sung_ve_an_toan_nito_long\"><\/span>Ba hi\u1ec3u l\u1ea7m b\u1ed5 sung v\u1ec1 an to\u00e0n nit\u01a1 l\u1ecfng<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><strong>&#8220;Nit\u01a1 kh\u00f4ng \u0111\u1ed9c n\u00ean ph\u00f2ng LN\u2082 kh\u00f4ng nguy hi\u1ec3m&#8221;<\/strong><\/p>\n\n\n\n<p>Kh\u00ed nit\u01a1 kh\u00f4ng c\u00f3 \u0111\u1ed9c t\u00ednh h\u00f3a h\u1ecdc theo ngh\u0129a th\u00f4ng th\u01b0\u1eddng, nh\u01b0ng c\u00f3 th\u1ec3 thay th\u1ebf oxy trong kh\u00f4ng kh\u00ed. OSHA \u0111\u00e3 ghi nh\u1eadn c\u00e1c s\u1ef1 c\u1ed1 thi\u1ebfu oxy nghi\u00eam tr\u1ecdng li\u00ean quan \u0111\u1ebfn vi\u1ec7c gi\u1ea3i ph\u00f3ng v\u00e0 n\u1ea1p nit\u01a1 l\u1ecfng trong kh\u00f4ng gian k\u00edn (theo <a href=\"https:\/\/www.osha.gov\/ords\/imis\/generalsearch.citation_detail?id=1448201.015&amp;cit_id=02002\">OSHA \u2013 Citation 1448201.015\/02002<\/a>).<\/p>\n\n\n\n<p>Khu v\u1ef1c Cryogenic Storage c\u1ea7n \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 v\u1ec1: th\u1ec3 t\u00edch ph\u00f2ng; t\u1ed5ng l\u01b0\u1ee3ng LN\u2082; k\u1ecbch b\u1ea3n r\u00f2 r\u1ec9; th\u00f4ng gi\u00f3; oxygen monitoring; c\u1ea3nh b\u00e1o b\u00ean trong v\u00e0 b\u00ean ngo\u00e0i; l\u1ed1i tho\u00e1t hi\u1ec3m; \u0111\u00e0o t\u1ea1o nh\u00e2n vi\u00ean.<\/p>\n\n\n\n<p><strong>&#8220;C\u00f3 s\u01b0\u01a1ng tr\u1eafng ngh\u0129a l\u00e0 c\u00f3 th\u1ec3 nh\u1eadn bi\u1ebft ch\u1ed7 thi\u1ebfu oxy&#8221;<\/strong><\/p>\n\n\n\n<p>S\u01b0\u01a1ng tr\u1eafng ch\u1ee7 y\u1ebfu l\u00e0 h\u01a1i n\u01b0\u1edbc trong kh\u00f4ng kh\u00ed ng\u01b0ng t\u1ee5 khi g\u1eb7p kh\u00ed l\u1ea1nh. Kh\u00ed nit\u01a1 kh\u00f4ng m\u00e0u v\u00e0 kh\u00f4ng m\u00f9i. Khu v\u1ef1c c\u00f3 th\u1ec3 thi\u1ebfu oxy ngay c\u1ea3 khi kh\u00f4ng nh\u00ecn th\u1ea5y s\u01b0\u01a1ng.<\/p>\n\n\n\n<p><strong>&#8220;G\u0103ng tay th\u00f4ng th\u01b0\u1eddng \u0111\u1ee7 \u0111\u1ec3 thao t\u00e1c v\u1edbi LN\u2082&#8221;<\/strong><\/p>\n\n\n\n<p>Ti\u1ebfp x\u00fac v\u1edbi LN\u2082 ho\u1eb7c b\u1ec1 m\u1eb7t cryogenic c\u00f3 th\u1ec3 g\u00e2y t\u1ed5n th\u01b0\u01a1ng l\u1ea1nh nghi\u00eam tr\u1ecdng. OSHA khuy\u1ebfn ngh\u1ecb tr\u00e1nh ti\u1ebfp x\u00fac tr\u1ef1c ti\u1ebfp v\u00e0 s\u1eed d\u1ee5ng thi\u1ebft b\u1ecb b\u1ea3o h\u1ed9 ph\u00f9 h\u1ee3p cho cryogen, bao g\u1ed3m b\u1ea3o v\u1ec7 tay v\u00e0 m\u1eaft theo \u0111\u00e1nh gi\u00e1 nguy c\u01a1 (theo <a href=\"https:\/\/www.osha.gov\/sites\/default\/files\/publications\/OSHAQUICKFACTS-LAB-SAFETY-CRYOGENS-DRYICE.pdf\">OSHA \u2013 Laboratory Safety: Cryogens and Dry Ice<\/a>).<\/p>\n\n\n\n<p>[\u1ea2NH MINH H\u1eccA] Nh\u00e2n vi\u00ean ph\u00f2ng lab s\u1eed d\u1ee5ng thi\u1ebft b\u1ecb b\u1ea3o h\u1ed9 cryogenic khi thao t\u00e1c v\u1edbi nit\u01a1 l\u1ecfng<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">T\u1eeb &#8220;m\u1ed9t chi\u1ebfc b\u00ecnh&#8221; \u0111\u1ebfn m\u1ed9t h\u1ec7 th\u1ed1ng qu\u1ea3n l\u00fd ch\u1ea5t l\u01b0\u1ee3ng<\/h2>\n\n\n\n<p>M\u01b0\u1eddi hi\u1ec3u l\u1ea7m tr\u00ean c\u00f3 c\u00f9ng m\u1ed9t nguy\u00ean nh\u00e2n g\u1ed1c r\u1ec5: xem Cryogenic Storage l\u00e0 m\u1ed9t thi\u1ebft b\u1ecb \u0111\u01a1n l\u1ebb.<\/p>\n\n\n\n<p>Trong th\u1ef1c t\u1ebf, m\u1ed9t h\u1ec7 th\u1ed1ng \u0111\u00e1ng tin c\u1eady c\u1ea7n \u00edt nh\u1ea5t n\u0103m l\u1edbp ki\u1ec3m so\u00e1t.<\/p>\n\n\n\n<p><strong>1. Khoa h\u1ecdc cryopreservation<\/strong> \u2014 ch\u1ea5t l\u01b0\u1ee3ng t\u1ebf b\u00e0o \u0111\u1ea7u v\u00e0o; cryoprotectant; t\u1ed1c \u0111\u1ed9 l\u00e0m l\u1ea1nh; nhi\u1ec7t \u0111\u1ed9 chuy\u1ec3n m\u1eabu; thawing protocol; QC sau r\u00e3 \u0111\u00f4ng.<\/p>\n\n\n\n<p><strong>2. Thi\u1ebft b\u1ecb v\u00e0 h\u1ea1 t\u1ea7ng<\/strong> \u2014 b\u00ecnh l\u01b0u tr\u1eef ph\u00f9 h\u1ee3p; Vapor ho\u1eb7c Liquid Phase; hi\u1ec7u qu\u1ea3 ch\u00e2n kh\u00f4ng; h\u1ec7 th\u1ed1ng c\u1ea5p LN\u2082; Auto Fill; th\u00f4ng gi\u00f3; oxygen monitoring.<\/p>\n\n\n\n<p><strong>3. Monitoring v\u00e0 alarm<\/strong> \u2014 nhi\u1ec7t \u0111\u1ed9; m\u1ee9c LN\u2082; ngu\u1ed3n \u0111i\u1ec7n; l\u1ecbch s\u1eed s\u1ef1 ki\u1ec7n; c\u1ea3nh b\u00e1o t\u1eeb xa; escalation.<\/p>\n\n\n\n<p><strong>4. Qu\u1ea3n l\u00fd m\u1eabu v\u00e0 d\u1eef li\u1ec7u<\/strong> \u2014 m\u00e3 \u0111\u1ecbnh danh duy nh\u1ea5t; barcode ho\u1eb7c m\u00e3 2D; v\u1ecb tr\u00ed ch\u00ednh x\u00e1c; audit trail; chain of identity; chain of custody; LIMS ho\u1eb7c h\u1ec7 th\u1ed1ng qu\u1ea3n l\u00fd ph\u00f9 h\u1ee3p.<\/p>\n\n\n\n<p><strong>5. H\u1ec7 th\u1ed1ng ch\u1ea5t l\u01b0\u1ee3ng v\u00e0 d\u1ef1 ph\u00f2ng<\/strong> \u2014 SOP; \u0111\u00e0o t\u1ea1o; preventive maintenance; calibration; deviation; CAPA; change control; emergency response; business continuity.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Checklist nhanh: C\u01a1 s\u1edf c\u1ee7a b\u1ea1n \u0111ang tin v\u00e0o &#8220;Myth&#8221; n\u00e0o?<\/h2>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>C\u00e2u h\u1ecfi<\/strong><\/td><td><strong>D\u1ea5u hi\u1ec7u c\u1ea7n xem x\u00e9t<\/strong><\/td><\/tr><tr><td>M\u1eabu c\u00f3 \u0111\u01b0\u1ee3c \u0111\u1ec3 \u1edf -80\u00b0C l\u00e2u h\u01a1n SOP kh\u00f4ng?<\/td><td>Ch\u01b0a ki\u1ec3m so\u00e1t th\u1eddi gian chuy\u1ec3n m\u1eabu<\/td><\/tr><tr><td>Nh\u00e2n vi\u00ean c\u00f3 ph\u1ea3i t\u00ecm m\u1eabu th\u1ee7 c\u00f4ng kh\u00f4ng?<\/td><td>Inventory ch\u01b0a hi\u1ec7u qu\u1ea3<\/td><\/tr><tr><td>Ch\u1ec9 theo d\u00f5i m\u1ee9c LN\u2082 m\u00e0 kh\u00f4ng theo d\u00f5i nhi\u1ec7t \u0111\u1ed9?<\/td><td>Thi\u1ebfu d\u1eef li\u1ec7u v\u00f9ng m\u1eabu<\/td><\/tr><tr><td>Alarm c\u00f3 \u0111\u01b0\u1ee3c th\u1eed \u0111\u1ecbnh k\u1ef3 kh\u00f4ng?<\/td><td>Nguy c\u01a1 c\u1ea3nh b\u00e1o kh\u00f4ng ho\u1ea1t \u0111\u1ed9ng<\/td><\/tr><tr><td>C\u00f3 theo d\u00f5i xu h\u01b0\u1edbng ti\u00eau hao LN\u2082 kh\u00f4ng?<\/td><td>C\u00f3 th\u1ec3 b\u1ecf s\u00f3t suy gi\u1ea3m c\u00e1ch nhi\u1ec7t<\/td><\/tr><tr><td>Viability l\u00e0 ch\u1ec9 ti\u00eau QC duy nh\u1ea5t?<\/td><td>Ch\u01b0a \u0111\u00e1nh gi\u00e1 \u0111\u1ea7y \u0111\u1ee7 ch\u1ee9c n\u0103ng<\/td><\/tr><tr><td>C\u00f3 b\u00ecnh d\u1ef1 ph\u00f2ng \u0111\u1ee7 dung l\u01b0\u1ee3ng kh\u00f4ng?<\/td><td>K\u1ebf ho\u1ea1ch s\u1ef1 c\u1ed1 ch\u01b0a ho\u00e0n ch\u1ec9nh<\/td><\/tr><tr><td>V\u1eadt ch\u1ee9a c\u00f3 ph\u00f9 h\u1ee3p v\u1edbi pha l\u01b0u tr\u1eef kh\u00f4ng?<\/td><td>Nguy c\u01a1 r\u00f2 r\u1ec9 ho\u1eb7c nhi\u1ec5m ch\u00e9o<\/td><\/tr><tr><td>C\u00f3 oxygen monitoring kh\u00f4ng?<\/td><td>R\u1ee7i ro an to\u00e0n nh\u00e2n vi\u00ean<\/td><\/tr><tr><td>C\u00f3 di\u1ec5n t\u1eadp chuy\u1ec3n m\u1eabu kh\u1ea9n c\u1ea5p kh\u00f4ng?<\/td><td>SOP ch\u01b0a \u0111\u01b0\u1ee3c ki\u1ec3m ch\u1ee9ng th\u1ef1c t\u1ebf<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Ket_luan\"><\/span>K\u1ebft lu\u1eadn<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>B\u1ea3o qu\u1ea3n l\u1ea1nh s\u00e2u kh\u00f4ng ph\u1ea3i l\u00e0 qu\u00e1 tr\u00ecnh &#8220;\u0111\u00f4ng l\u1ea1nh r\u1ed3i qu\u00ean \u0111i&#8221;. M\u1eabu v\u1eabn c\u1ea7n \u0111\u01b0\u1ee3c b\u1ea3o v\u1ec7 kh\u1ecfi dao \u0111\u1ed9ng nhi\u1ec7t, l\u1ed7i thi\u1ebft b\u1ecb, sai s\u00f3t nh\u1eadn d\u1ea1ng, nhi\u1ec5m ch\u00e9o, gi\u00e1n \u0111o\u1ea1n ngu\u1ed3n LN\u2082 v\u00e0 c\u00e1c v\u1ea5n \u0111\u1ec1 d\u1eef li\u1ec7u trong su\u1ed1t v\u00f2ng \u0111\u1eddi l\u01b0u tr\u1eef.<\/p>\n\n\n\n<p>M\u01b0\u1eddi s\u1ef1 th\u1eadt quan tr\u1ecdng c\u1ea7n ghi nh\u1edb l\u00e0:<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Nhi\u1ec7t \u0111\u1ed9 th\u1ea5p kh\u00f4ng th\u1ec3 s\u1eeda m\u1ed9t quy tr\u00ecnh \u0111\u00f4ng l\u1ea1nh sai.<\/li>\n\n\n\n<li>-80\u00b0C th\u01b0\u1eddng kh\u00f4ng ph\u00f9 h\u1ee3p cho l\u01b0u tr\u1eef d\u00e0i h\u1ea1n c\u1ee7a nhi\u1ec1u t\u1ebf b\u00e0o s\u1ed1ng.<\/li>\n\n\n\n<li>M\u1eabu ch\u01b0a r\u00e3 \u0111\u00f4ng v\u1eabn c\u00f3 th\u1ec3 b\u1ecb \u1ea3nh h\u01b0\u1edfng b\u1edfi chu k\u1ef3 nhi\u1ec7t.<\/li>\n\n\n\n<li>Vapor Phase v\u00e0 Liquid Phase \u0111\u1ec1u ph\u1ea3i \u0111\u01b0\u1ee3c l\u1ef1a ch\u1ecdn theo \u0111\u00e1nh gi\u00e1 r\u1ee7i ro.<\/li>\n\n\n\n<li>Nit\u01a1 l\u1ecfng kh\u00f4ng \u0111\u01b0\u1ee3c m\u1eb7c \u0111\u1ecbnh l\u00e0 v\u00f4 tr\u00f9ng.<\/li>\n\n\n\n<li>B\u00ecnh c\u00f2n LN\u2082 ch\u01b0a ch\u1ee9ng minh m\u1ecdi m\u1eabu \u0111\u1ec1u an to\u00e0n.<\/li>\n\n\n\n<li>M\u1ea5t \u0111i\u1ec7n v\u1eabn c\u00f3 th\u1ec3 l\u00e0m m\u1ea5t nhi\u1ec1u l\u1edbp b\u1ea3o v\u1ec7.<\/li>\n\n\n\n<li>Alarm ch\u1ec9 hi\u1ec7u qu\u1ea3 khi c\u00f3 quy tr\u00ecnh ph\u1ea3n \u1ee9ng.<\/li>\n\n\n\n<li>Viability kh\u00f4ng \u0111\u1ea1i di\u1ec7n cho to\u00e0n b\u1ed9 ch\u1ea5t l\u01b0\u1ee3ng.<\/li>\n\n\n\n<li>Cryogenic Storage v\u1eabn c\u1ea7n b\u1ea3o tr\u00ec d\u1ef1 ph\u00f2ng.<\/li>\n<\/ol>\n\n\n\n<p>\u0110\u1ed1i v\u1edbi Cell Therapy, Biobank, IVF v\u00e0 ng\u00e2n h\u00e0ng t\u1ebf b\u00e0o g\u1ed1c, Cryogenic Storage ph\u1ea3i \u0111\u01b0\u1ee3c qu\u1ea3n l\u00fd nh\u01b0 m\u1ed9t h\u1ea1 t\u1ea7ng ch\u1ea5t l\u01b0\u1ee3ng tr\u1ecdng y\u1ebfu, n\u01a1i thi\u1ebft b\u1ecb, con ng\u01b0\u1eddi, d\u1eef li\u1ec7u v\u00e0 quy tr\u00ecnh c\u00f9ng ch\u1ecbu tr\u00e1ch nhi\u1ec7m b\u1ea3o v\u1ec7 c\u00e1c t\u00e0i s\u1ea3n sinh h\u1ecdc kh\u00f4ng th\u1ec3 thay th\u1ebf.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p class=\"has-background\" style=\"background:linear-gradient(135deg,rgb(234,246,252) 0%,rgb(234,246,252) 100%)\">H\u1ec7 th\u1ed1ng Cryogenic Storage c\u1ee7a b\u1ea1n \u0111ang \u0111\u01b0\u1ee3c ki\u1ec3m so\u00e1t b\u1eb1ng d\u1eef li\u1ec7u hay b\u1eb1ng gi\u1ea3 \u0111\u1ecbnh?<br><br>Biogroup Vietnam cung c\u1ea5p gi\u1ea3i ph\u00e1p Cryogenic Storage to\u00e0n di\u1ec7n cho Cell Therapy, Biobank, IVF, ng\u00e2n h\u00e0ng m\u00e1u cu\u1ed1ng r\u1ed1n v\u00e0 ph\u00f2ng th\u00ed nghi\u1ec7m nghi\u00ean c\u1ee9u, bao g\u1ed3m:<br><br>\u2022 Kh\u1ea3o s\u00e1t hi\u1ec7n tr\u1ea1ng v\u00e0 \u0111\u00e1nh gi\u00e1 r\u1ee7i ro;<br>\u2022 T\u01b0 v\u1ea5n Vapor Phase ho\u1eb7c Liquid Phase;<br>\u2022 H\u1ec7 th\u1ed1ng b\u00ecnh l\u01b0u tr\u1eef MVE;<br>\u2022 Thi\u1ebft k\u1ebf dung l\u01b0\u1ee3ng, rack v\u00e0 inventory;<br>\u2022 H\u1ec7 th\u1ed1ng c\u1ea5p LN\u2082 v\u00e0 Auto Fill;<br>\u2022 Monitoring nhi\u1ec7t \u0111\u1ed9, m\u1ee9c LN\u2082 v\u00e0 n\u1ed3ng \u0111\u1ed9 oxy;<br>\u2022 Thi\u1ebft k\u1ebf alarm escalation;<br>\u2022 Preventive maintenance;<br>\u2022 \u0110\u00e0o t\u1ea1o v\u1eadn h\u00e0nh v\u00e0 \u1ee9ng ph\u00f3 s\u1ef1 c\u1ed1;<br>\u2022 T\u01b0 v\u1ea5n k\u1ebf ho\u1ea1ch d\u1ef1 ph\u00f2ng v\u00e0 chuy\u1ec3n m\u1eabu kh\u1ea9n c\u1ea5p.<br><br><strong>\ud83d\udce9 Li\u00ean h\u1ec7 Biogroup Vietnam<\/strong> \u0111\u1ec3 th\u1ef1c hi\u1ec7n Cryogenic Storage Health Check, nh\u1eadn di\u1ec7n c\u00e1c gi\u1ea3 \u0111\u1ecbnh ch\u01b0a \u0111\u01b0\u1ee3c ki\u1ec3m ch\u1ee9ng v\u00e0 x\u00e2y d\u1ef1ng h\u1ec7 th\u1ed1ng l\u01b0u tr\u1eef l\u1ea1nh s\u00e2u an to\u00e0n, c\u00f3 kh\u1ea3 n\u0103ng truy xu\u1ea5t v\u00e0 ph\u00f9 h\u1ee3p v\u1edbi \u0111\u1ecbnh h\u01b0\u1edbng ph\u00e1t tri\u1ec3n d\u00e0i h\u1ea1n:<br><br>\u2022 Website: <a href=\"https:\/\/biogroupvietnam.com\/vi\/lien-he\/\">https:\/\/biogroupvietnam.com\/vi\/lien-he\/<\/a><br>\u2022 Hotline: +84 963 621 421<br>\u2022 Email: info@biogroupvietnam.com<\/p>\n\n\n\n<p><br><strong>T\u00c0I LI\u1ec6U THAM KH\u1ea2O<\/strong><\/p>\n\n\n\n<ol start=\"1\" class=\"wp-block-list\">\n<li>Whaley D, Damyar K, Witek RP, Mendoza A, Alexander M, Lakey JRT. <em>Cryopreservation: An Overview of Principles and Cell-Specific Considerations.<\/em> Frontiers\/PubMed. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33757335\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/33757335\/<\/a><\/li>\n\n\n\n<li>STEMCELL Technologies. <em>Cryopreservation Basics: Protocols and Best Practices for Freezing Cells.<\/em> <a href=\"https:\/\/www.stemcell.com\/cryopreservation-basics-protocols-and-best-practices-for-freezing-cells\">https:\/\/www.stemcell.com\/cryopreservation-basics-protocols-and-best-practices-for-freezing-cells<\/a><\/li>\n\n\n\n<li>Pogozhykh D, Pogozhykh O, Prokopyuk V, Kuleshova L, Goltsev A, Blasczyk R, M\u00fcller T. (2017). <em>Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cells.<\/em> Stem Cell Res Ther. PMID: 28284229. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28284229\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/28284229\/<\/a><\/li>\n\n\n\n<li>Okuda J, Watanabe N, Nakamura T, Mizushima K, Xi H, Kumamoto Y, Fujita K, Kino-Oka M. (2024). <em>The impact of repeated temperature cycling on cryopreserved human iPSC viability stems from cytochrome redox state changes.<\/em> Front Bioeng Biotechnol. PMID: 39139293. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39139293\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/39139293\/<\/a><\/li>\n\n\n\n<li>Bajerski F, Nagel M, Overmann J. (2021). <em>Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?<\/em> Appl Microbiol Biotechnol. PMID: 34559283. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34559283\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/34559283\/<\/a><\/li>\n\n\n\n<li>Joaquim DC, Borges ED, Viana IGR, Navarro PA, Vireque AA. (2017). <em>Risk of Contamination of Gametes and Embryos during Cryopreservation and Measures to Prevent Cross-Contamination.<\/em> Biomed Res Int. PMID: 28890894. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28890894\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/28890894\/<\/a><\/li>\n\n\n\n<li>Meneghel J, Kilbride P, Morris GJ. (2020). <em>Cryopreservation as a Key Element in the Successful Delivery of Cell-Based Therapies \u2013 A Review.<\/em> Frontiers in Medicine. <a href=\"https:\/\/www.frontiersin.org\/journals\/medicine\/articles\/10.3389\/fmed.2020.592242\/full\">https:\/\/www.frontiersin.org\/journals\/medicine\/articles\/10.3389\/fmed.2020.592242\/full<\/a><\/li>\n\n\n\n<li>ISBER (International Society for Biological and Environmental Repositories). <em>Best Practices for Repositories, 5th Edition.<\/em> <a href=\"https:\/\/www.isber.org\/page\/BPR\">https:\/\/www.isber.org\/page\/BPR<\/a><\/li>\n\n\n\n<li>OSHA (Occupational Safety and Health Administration). <em>Citation 1448201.015\/02002.<\/em> <a href=\"https:\/\/www.osha.gov\/ords\/imis\/generalsearch.citation_detail?id=1448201.015&amp;cit_id=02002\">https:\/\/www.osha.gov\/ords\/imis\/generalsearch.citation_detail?id=1448201.015&amp;cit_id=02002<\/a><\/li>\n\n\n\n<li>OSHA. <em>Laboratory Safety: Cryogens and Dry Ice (OSHA 3408).<\/em> <a href=\"https:\/\/www.osha.gov\/sites\/default\/files\/publications\/OSHAQUICKFACTS-LAB-SAFETY-CRYOGENS-DRYICE.pdf\">https:\/\/www.osha.gov\/sites\/default\/files\/publications\/OSHAQUICKFACTS-LAB-SAFETY-CRYOGENS-DRYICE.pdf<\/a><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>B\u1ea3o qu\u1ea3n l\u1ea1nh s\u00e2u \u2013 Cryogenic Storage \u2013 l\u00e0 n\u1ec1n t\u1ea3ng quan tr\u1ecdng trong Cell Therapy, Biobank, IVF v\u00e0 ng\u00e2n h\u00e0ng m\u00e1u cu\u1ed1ng r\u1ed1n, c\u0169ng nh\u01b0 trong nghi\u00ean c\u1ee9u khoa h\u1ecdc s\u1ef1 s\u1ed1ng. Tuy nhi\u00ean, \u0111\u00e2y c\u0169ng l\u00e0 l\u0129nh v\u1ef1c t\u1ed3n t\u1ea1i nhi\u1ec1u c\u00e1ch hi\u1ec3u \u0111\u01a1n gi\u1ea3n h\u00f3a. M\u1ed9t s\u1ed1 quan ni\u1ec7m th\u01b0\u1eddng g\u1eb7p g\u1ed3m:<\/p>\n<div class=\"klb-readmore post-buttons\"><a class=\"button rounded primary\" href=\"https:\/\/biogroupvietnam.com\/en\/blog\/10-hieu-lam-pho-bien-ve-luu-tru-lanh-sau\/\">Read More <i class=\"klbth-icon-right-arrow\"><\/i><\/a><\/div>\n","protected":false},"author":1,"featured_media":11476,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-11474","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>10 hi\u1ec3u l\u1ea7m ph\u1ed5 bi\u1ebfn v\u1ec1 b\u1ea3o qu\u1ea3n l\u1ea1nh s\u00e2u<\/title>\n<meta name=\"description\" content=\"Gi\u1ea3i \u0111\u00e1p 10 hi\u1ec3u l\u1ea7m v\u1ec1 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