{"id":4398,"date":"2024-02-25T08:50:23","date_gmt":"2024-02-25T08:50:23","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/service\/speeding-up-the-qpcr-reaction-preparation-process-in-diagnosing-sars-cov-2-with-the-ezmate-601-system\/"},"modified":"2026-04-14T16:42:39","modified_gmt":"2026-04-14T09:42:39","slug":"tang-toc-qua-trinh-chuan-bi-phan-ung-qpcr-trong-chan-doan-sars-cov-2-voi-he-thong-ezmate-601","status":"publish","type":"service","link":"https:\/\/biogroupvietnam.com\/en\/service\/speeding-up-the-qpcr-reaction-preparation-process-in-diagnosing-sars-cov-2-with-the-ezmate-601-system\/","title":{"rendered":"Speeding up the qPCR reaction preparation process in diagnosing SARS-CoV-2 with the EzMate 601s System"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/en\/service\/speeding-up-the-qpcr-reaction-preparation-process-in-diagnosing-sars-cov-2-with-the-ezmate-601-system\/#Operational_needs_of_high-throughput_diagnostic_laboratories\" >Operational needs of high-throughput diagnostic laboratories<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/en\/service\/speeding-up-the-qpcr-reaction-preparation-process-in-diagnosing-sars-cov-2-with-the-ezmate-601-system\/#Recommendations_to_accelerate_qPCR_reaction_setup_for_diagnostics\" >Recommendations to accelerate qPCR reaction setup for diagnostics<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/biogroupvietnam.com\/en\/service\/speeding-up-the-qpcr-reaction-preparation-process-in-diagnosing-sars-cov-2-with-the-ezmate-601-system\/#Automated_master_mix_preparation_using_the_EzMate_601s\" >Automated master mix preparation using the EzMate 601s<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/biogroupvietnam.com\/en\/service\/speeding-up-the-qpcr-reaction-preparation-process-in-diagnosing-sars-cov-2-with-the-ezmate-601-system\/#Manual_master_mix_preparation\" >Manual master mix preparation<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/biogroupvietnam.com\/en\/service\/speeding-up-the-qpcr-reaction-preparation-process-in-diagnosing-sars-cov-2-with-the-ezmate-601-system\/#Additional_applications\" >Additional applications<\/a><\/li><\/ul><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"Operational_needs_of_high-throughput_diagnostic_laboratories\"><\/span>Operational needs of high-throughput diagnostic laboratories<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>During the SARS-CoV-2 pandemic, a customer of Blue-Ray Biotech planned to implement automation solutions for diagnostic laboratories. These included workflows such as viral RNA isolation\/extraction and the preparation of COVID-19 diagnostic assays based on Real-Time PCR. The customer requested support from Blue-Ray Biotech to design an optimized method for qPCR assay setup. The following are the solutions proposed by Blue-Ray Biotech.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Recommendations_to_accelerate_qPCR_reaction_setup_for_diagnostics\"><\/span>Recommendations to accelerate qPCR reaction setup for diagnostics<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<h3><span class=\"ez-toc-section\" id=\"Automated_master_mix_preparation_using_the_EzMate_601s\"><\/span>Automated master mix preparation using the EzMate 601s<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>If users choose to prepare the Master Mix automatically (19 \u00b5L Super Mix + 1 \u00b5L Enzyme Mix), the <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/thiet-bi-sinh-hoc-phan-tu\/he-thong-xet-nghiem-pcr\/he-thong-pipette-tu-dong.html\">EzMate 601s<\/a> Automated Pipetting System can process up to 50 reactions per run (equivalent to 2 plates of a Real-Time PCR kit).<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Manual_master_mix_preparation\"><\/span>Manual master mix preparation<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>If users prefer to prepare the Master Mix solution manually (19 \u00b5L Super Mix + 1 \u00b5L Enzyme Mix), the <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/thiet-bi-sinh-hoc-phan-tu\/he-thong-xet-nghiem-pcr\/he-thong-pipette-tu-dong.html\">EzMate 601s<\/a> system can process up to 75 reactions per run (equivalent to 3 sample plates of a Real-Time RT-PCR kit).<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Additional_applications\"><\/span>Additional applications<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>Blue-Ray Biotech also offers customized automated sample preparation workflows tailored to specific user requirements.<\/p>\n<p><strong>REFERENCE<\/strong><\/p>\n<p>Blue-Ray Biotech, <em data-start=\"1088\" data-end=\"1194\">Application Note: Accelerate qPCR Setup for COVID-19 Diagnosis on EzMate 601s Automated Pipetting System <a class=\"decorated-link\" href=\"https:\/\/www.blue-raybio.com\/en\/resource\/detail\/Accelerate-qPCR-Setup-for-COVID-19-Diagnosis-on-EzMate-601s-Automated-Pipetting-System\" target=\"_new\" rel=\"noopener\" data-start=\"687\" data-end=\"820\">https:\/\/www.blue-raybio.com\/en\/resource\/detail\/Accelerate-qPCR-Setup-for-COVID-19-Diagnosis-on-EzMate-601s-Automated-Pipetting-System<\/a><\/em><\/p>\n","protected":false},"featured_media":5892,"parent":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-4398","service","type-service","status-publish","has-post-thumbnail","hentry","service-category-procedures-directions-for-use"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Speeding up the qPCR reaction preparation process in diagnosing SARS-CoV-2 with the EzMate 601s System<\/title>\n<meta name=\"description\" content=\"Operational needs of high-throughput diagnostic laboratories During the SARS-CoV-2 pandemic, a customer of Blue-Ray Biotech planned to implement automation solutions for diagnostic laboratories. 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