{"id":4398,"date":"2024-02-25T08:50:23","date_gmt":"2024-02-25T08:50:23","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/service\/speeding-up-the-qpcr-reaction-preparation-process-in-diagnosing-sars-cov-2-with-the-ezmate-601-system\/"},"modified":"2026-04-21T12:52:49","modified_gmt":"2026-04-21T05:52:49","slug":"tang-toc-qua-trinh-chuan-bi-phan-ung-qpcr-trong-chan-doan-sars-cov-2-voi-he-thong-ezmate-601","status":"publish","type":"service","link":"https:\/\/biogroupvietnam.com\/en\/service\/speeding-up-the-qpcr-reaction-preparation-process-in-diagnosing-sars-cov-2-with-the-ezmate-601-system\/","title":{"rendered":"Speeding up the qPCR reaction preparation process in diagnosing SARS-CoV-2 with the EzMate 601s System"},"content":{"rendered":"<h2>Operational needs of high-throughput diagnostic laboratories<\/h2>\n<p>During the SARS-CoV-2 pandemic, a customer of Blue-Ray Biotech planned to implement automation solutions for diagnostic laboratories. These included workflows such as viral RNA isolation\/extraction and the preparation of COVID-19 diagnostic assays based on Real-Time PCR. The customer requested support from Blue-Ray Biotech to design an optimized method for qPCR assay setup. The following are the solutions proposed by Blue-Ray Biotech.<\/p>\n<h2>Recommendations to accelerate qPCR reaction setup for diagnostics<\/h2>\n<h3>Automated master mix preparation using the EzMate 601s<\/h3>\n<p>If users choose to prepare the Master Mix automatically (19 \u00b5L Super Mix + 1 \u00b5L Enzyme Mix), the <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/thiet-bi-sinh-hoc-phan-tu\/he-thong-xet-nghiem-pcr\/he-thong-pipette-tu-dong.html\">EzMate 601s<\/a> Automated Pipetting System can process up to 50 reactions per run (equivalent to 2 plates of a Real-Time PCR kit).<\/p>\n<h3>Manual master mix preparation<\/h3>\n<p>If users prefer to prepare the Master Mix solution manually (19 \u00b5L Super Mix + 1 \u00b5L Enzyme Mix), the <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/thiet-bi-sinh-hoc-phan-tu\/he-thong-xet-nghiem-pcr\/he-thong-pipette-tu-dong.html\">EzMate 601s<\/a> system can process up to 75 reactions per run (equivalent to 3 sample plates of a Real-Time RT-PCR kit).<\/p>\n<h3>Additional applications<\/h3>\n<p>Blue-Ray Biotech also offers customized automated sample preparation workflows tailored to specific user requirements.<\/p>\n<p><strong>REFERENCE<\/strong><\/p>\n<p>Blue-Ray Biotech, <em data-start=\"1088\" data-end=\"1194\">Application Note: Accelerate qPCR Setup for COVID-19 Diagnosis on EzMate 601s Automated Pipetting System <a class=\"decorated-link\" href=\"https:\/\/www.blue-raybio.com\/en\/resource\/detail\/Accelerate-qPCR-Setup-for-COVID-19-Diagnosis-on-EzMate-601s-Automated-Pipetting-System\" target=\"_new\" rel=\"noopener\" data-start=\"687\" data-end=\"820\">https:\/\/www.blue-raybio.com\/en\/resource\/detail\/Accelerate-qPCR-Setup-for-COVID-19-Diagnosis-on-EzMate-601s-Automated-Pipetting-System<\/a><\/em><\/p>\n","protected":false},"featured_media":5892,"parent":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-4398","service","type-service","status-publish","has-post-thumbnail","hentry","service-category-procedures-directions-for-use"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Speeding up the qPCR reaction preparation process in diagnosing SARS-CoV-2 with the EzMate 601s System<\/title>\n<meta name=\"description\" content=\"Operational needs of high-throughput diagnostic laboratories During the SARS-CoV-2 pandemic, a customer of Blue-Ray Biotech planned to implement automation solutions for diagnostic laboratories. These included workflows such as viral RNA isolation\/extraction and the preparation of COVID-19 diagnostic assays based on Real-Time PCR. 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These included workflows such as viral RNA isolation\/extraction and the preparation of COVID-19 diagnostic assays based on Real-Time PCR. 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