{"id":10256,"date":"2026-04-08T18:08:16","date_gmt":"2026-04-08T11:08:16","guid":{"rendered":"https:\/\/biogroupvietnam.com\/?p=10256"},"modified":"2026-04-22T14:20:02","modified_gmt":"2026-04-22T07:20:02","slug":"cac-phuong-phap-dinh-luong-axit-nucleic","status":"publish","type":"post","link":"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/","title":{"rendered":"C\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic: H\u01b0\u1edbng d\u1eabn to\u00e0n di\u1ec7n"},"content":{"rendered":"\n<p>Ph\u01b0\u01a1ng ph\u00e1p n\u00e0o l\u00e0 l\u1ef1a ch\u1ecdn t\u1ed1i \u01b0u \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic? Kh\u00f4ng c\u00f3 c\u00e2u tr\u1ea3 l\u1eddi chung cho m\u1ecdi tr\u01b0\u1eddng h\u1ee3p, b\u1edfi m\u1ed7i k\u1ef9 thu\u1eadt \u0111\u1ec1u c\u00f3 \u0111\u1eb7c \u0111i\u1ec3m ri\u00eang. B\u00e0i vi\u1ebft n\u00e0y s\u1ebd gi\u00fap b\u1ea1n hi\u1ec3u r\u00f5 5 ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic ph\u1ed5 bi\u1ebfn:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u0110o quang ph\u1ed5 UV-Vis cho \u0111\u00e1nh gi\u00e1 nhanh<\/li>\n\n\n\n<li>\u0110o hu\u1ef3nh quang cho \u0111\u1ed9 nh\u1ea1y cao v\u1edbi m\u1eabu n\u1ed3ng \u0111\u1ed9 th\u1ea5p<\/li>\n\n\n\n<li>Real-time qPCR cho \u0111\u1ecbnh l\u01b0\u1ee3ng ch\u00ednh x\u00e1c<\/li>\n\n\n\n<li>\u0110i\u1ec7n di gel cho ki\u1ec3m tra t\u00ednh to\u00e0n v\u1eb9n<\/li>\n\n\n\n<li>\u0110i\u1ec7n di mao qu\u1ea3n cho ph\u00e2n t\u00edch t\u1ef1 \u0111\u1ed9ng<\/li>\n<\/ul>\n\n\n\n<p>C\u00f9ng l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p ph\u00f9 h\u1ee3p theo nhu c\u1ea7u nghi\u00ean c\u1ee9u v\u00e0 y\u00eau c\u1ea7u v\u1ec1 \u0111\u1ed9 ch\u00ednh x\u00e1c m\u00e0 b\u1ea1n mong mu\u1ed1n.<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"576\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/04\/cac-phuong-phap-dinh-luong-axit-nucleic-1024x576.png\" alt=\"C\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic\" class=\"wp-image-10303\" srcset=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/04\/cac-phuong-phap-dinh-luong-axit-nucleic-1024x576.png 1024w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/04\/cac-phuong-phap-dinh-luong-axit-nucleic-300x169.png 300w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/04\/cac-phuong-phap-dinh-luong-axit-nucleic-768x432.png 768w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/04\/cac-phuong-phap-dinh-luong-axit-nucleic-600x338.png 600w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/04\/cac-phuong-phap-dinh-luong-axit-nucleic-90x51.png 90w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2026\/04\/cac-phuong-phap-dinh-luong-axit-nucleic.png 1422w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\"><em>C\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic<\/em><\/figcaption><\/figure><\/div>\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#Tam_quan_trong_cua_dinh_luong_axit_nucleic\" >T\u1ea7m quan tr\u1ecdng c\u1ee7a \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#Tong_quan_ve_cac_phuong_phap_dinh_luong_axit_nucleic\" >T\u1ed5ng quan v\u1ec1 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#Cac_phuong_phap_dinh_luong_axit_nucleic_chinh\" >C\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic ch\u00ednh<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#Do_quang_pho_UV-Vis_UV-Vis_Spectrophotometry\" >\u0110o quang ph\u1ed5 UV-Vis (UV-Vis Spectrophotometry)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#Do_huynh_quang_Fluorometry\" >\u0110o hu\u1ef3nh quang (Fluorometry)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#PCR_dinh_luong_thoi_gian_thuc_qPCR\" >PCR \u0111\u1ecbnh l\u01b0\u1ee3ng th\u1eddi gian th\u1ef1c (qPCR)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#Dien_di_gel\" >\u0110i\u1ec7n di gel<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#Dien_di_mao_quan_Capillary_Electrophoresis\" >\u0110i\u1ec7n di mao qu\u1ea3n (Capillary Electrophoresis)<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#Cach_lua_chon_phuong_phap_dinh_luong_DNA_phu_hop\" >C\u00e1ch l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng DNA ph\u00f9 h\u1ee3p<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#So_sanh_cac_phuong_phap_dinh_luong_axit_nucleic\" >So s\u00e1nh c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#Cau_hoi_thuong_gap_FAQ\" >C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p (FAQ)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-12\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/#Ket_luan\" >K\u1ebft lu\u1eadn<\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Tam_quan_trong_cua_dinh_luong_axit_nucleic\"><\/span><strong>T\u1ea7m quan tr\u1ecdng c\u1ee7a \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>Trong nghi\u00ean c\u1ee9u sinh h\u1ecdc ph\u00e2n t\u1eed hi\u1ec7n \u0111\u1ea1i, \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic l\u00e0 m\u1ed9t b\u01b0\u1edbc thi\u1ebft y\u1ebfu. Vi\u1ec7c \u0111\u1ecbnh l\u01b0\u1ee3ng ch\u00ednh x\u00e1c axit nucleic \u0111\u00f3ng vai tr\u00f2 then ch\u1ed1t trong vi\u1ec7c \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 tin c\u1eady v\u00e0 kh\u1ea3 n\u0103ng t\u00e1i l\u1eadp c\u1ee7a k\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m. X\u00e1c \u0111\u1ecbnh ch\u00ednh x\u00e1c n\u1ed3ng \u0111\u1ed9 axit nucleic trong m\u1eabu l\u00e0 y\u1ebfu t\u1ed1 quy\u1ebft \u0111\u1ecbnh th\u00e0nh c\u00f4ng c\u1ee7a PCR, gi\u1ea3i tr\u00ecnh t\u1ef1 gen ho\u1eb7c b\u1ea5t k\u1ef3 th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed n\u00e0o kh\u00e1c.<\/p>\n\n\n\n<p>T\u1ea7m quan tr\u1ecdng ch\u00ednh c\u1ee7a \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic th\u1ec3 hi\u1ec7n \u1edf vi\u1ec7c t\u1ed1i \u01b0u h\u00f3a \u0111i\u1ec1u ki\u1ec7n th\u00ed nghi\u1ec7m, \u0111\u00e1nh gi\u00e1 ch\u1ea5t l\u01b0\u1ee3ng m\u1eabu, \u0111\u1ea3m b\u1ea3o th\u00e0nh c\u00f4ng c\u1ee7a c\u00e1c \u1ee9ng d\u1ee5ng ti\u1ebfp theo, c\u0169ng nh\u01b0 ti\u1ebft ki\u1ec7m th\u1eddi gian v\u00e0 ngu\u1ed3n l\u1ef1c. Nhi\u1ec1u th\u00ed nghi\u1ec7m y\u00eau c\u1ea7u n\u1ed3ng \u0111\u1ed9 axit nucleic \u0111\u1ea7u v\u00e0o c\u1ee5 th\u1ec3, v\u00e0 vi\u1ec7c \u0111\u1ecbnh l\u01b0\u1ee3ng ch\u00ednh x\u00e1c gi\u00fap c\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u t\u1ed1i \u01b0u h\u00f3a \u0111i\u1ec1u ki\u1ec7n th\u00ed nghi\u1ec7m. Th\u00f4ng qua \u0111\u1ecbnh l\u01b0\u1ee3ng, c\u00f3 th\u1ec3 \u0111\u00e1nh gi\u00e1 hi\u1ec7u qu\u1ea3 c\u1ee7a qu\u00e1 tr\u00ecnh t\u00e1ch chi\u1ebft v\u00e0 tinh s\u1ea1ch DNA, \u0111\u1ed3ng th\u1eddi ph\u00e1t hi\u1ec7n c\u00e1c y\u1ebfu t\u1ed1 g\u00e2y nhi\u1ec5m. Trong c\u00e1c \u1ee9ng d\u1ee5ng nh\u01b0 NGS (Next Generation Sequencing &#8211; gi\u1ea3i tr\u00ecnh t\u1ef1 gen th\u1ebf h\u1ec7 m\u1edbi), \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic ch\u00ednh x\u00e1c l\u00e0 y\u1ebfu t\u1ed1 quan tr\u1ecdng \u0111\u1ed1i v\u1edbi qu\u00e1 tr\u00ecnh chu\u1ea9n b\u1ecb th\u01b0 vi\u1ec7n. Ngo\u00e0i ra, \u0111\u1ecbnh l\u01b0\u1ee3ng ch\u00ednh x\u00e1c c\u00f2n gi\u00fap tr\u00e1nh th\u1ea5t b\u1ea1i th\u00ed nghi\u1ec7m do n\u1ed3ng \u0111\u1ed9 axit nucleic kh\u00f4ng ph\u00f9 h\u1ee3p, t\u1eeb \u0111\u00f3 ti\u1ebft ki\u1ec7m th\u1eddi gian v\u00e0 t\u00e0i nguy\u00ean qu\u00fd gi\u00e1.<\/p>\n\n\n\n<p>V\u1edbi t\u1ea7m quan tr\u1ecdng \u0111\u00f3, vi\u1ec7c l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng ph\u00f9 h\u1ee3p tr\u1edf n\u00ean \u0111\u1eb7c bi\u1ec7t quan tr\u1ecdng. M\u1ed7i ph\u01b0\u01a1ng ph\u00e1p c\u00f3 nh\u1eefng \u01b0u \u0111i\u1ec3m v\u00e0 h\u1ea1n ch\u1ebf ri\u00eang, ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c b\u1ed1i c\u1ea3nh th\u00ed nghi\u1ec7m kh\u00e1c nhau.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Tong_quan_ve_cac_phuong_phap_dinh_luong_axit_nucleic\"><\/span><strong>T\u1ed5ng quan v\u1ec1 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>C\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u c\u00f3 th\u1ec3 l\u1ef1a ch\u1ecdn nhi\u1ec1u ph\u01b0\u01a1ng ph\u00e1p kh\u00e1c nhau \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic, m\u1ed7i ph\u01b0\u01a1ng ph\u00e1p \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf cho c\u00e1c nhu c\u1ea7u th\u1ef1c nghi\u1ec7m c\u1ee5 th\u1ec3 v\u00e0 d\u1ef1a tr\u00ean c\u00e1c nguy\u00ean l\u00fd kh\u00e1c nhau. Nh\u1eefng ph\u01b0\u01a1ng ph\u00e1p ph\u1ed5 bi\u1ebfn nh\u1ea5t bao g\u1ed3m \u0111o quang ph\u1ed5 UV-Vis, \u0111o hu\u1ef3nh quang, PCR \u0111\u1ecbnh l\u01b0\u1ee3ng th\u1eddi gian th\u1ef1c (qPCR), \u0111i\u1ec7n di gel v\u00e0 \u0111i\u1ec7n di mao qu\u1ea3n. C\u00e1c k\u1ef9 thu\u1eadt n\u00e0y kh\u00e1c nhau v\u1ec1 \u0111\u1ed9 ph\u1ee9c t\u1ea1p v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c, t\u1eeb ph\u01b0\u01a1ng ph\u00e1p UV-Vis nhanh ch\u00f3ng v\u00e0 \u0111\u01a1n gi\u1ea3n \u0111\u1ebfn qPCR c\u00f3 \u0111\u1ed9 ch\u00ednh x\u00e1c r\u1ea5t cao, cung c\u1ea5p nhi\u1ec1u l\u1ef1a ch\u1ecdn ph\u00f9 h\u1ee3p v\u1edbi y\u00eau c\u1ea7u nghi\u00ean c\u1ee9u.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Cac_phuong_phap_dinh_luong_axit_nucleic_chinh\"><\/span><strong>C\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic ch\u00ednh<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Do_quang_pho_UV-Vis_UV-Vis_Spectrophotometry\"><\/span>\u0110o quang ph\u1ed5 UV-Vis (UV-Vis Spectrophotometry)<span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p><a href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/may-do-quang-pho-vi-the-tich-uvvis-ezdrop-1000\/\">\u0110o quang ph\u1ed5 UV-Vis<\/a> l\u00e0 m\u1ed9t trong nh\u1eefng ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn nh\u1ea5t, n\u1ed5i b\u1eadt nh\u1edd t\u00ednh \u0111\u01a1n gi\u1ea3n v\u00e0 t\u1ed1c \u0111\u1ed9 nhanh. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y d\u1ef1a tr\u00ean \u0111\u1eb7c t\u00ednh h\u1ea5p th\u1ee5 c\u1ef1c \u0111\u1ea1i c\u1ee7a axit nucleic t\u1ea1i b\u01b0\u1edbc s\u00f3ng 260 nm. B\u1eb1ng c\u00e1ch \u0111o \u0111\u1ed9 h\u1ea5p th\u1ee5 c\u1ee7a m\u1eabu t\u1ea1i b\u01b0\u1edbc s\u00f3ng n\u00e0y, c\u00f3 th\u1ec3 t\u00ednh to\u00e1n n\u1ed3ng \u0111\u1ed9 axit nucleic. C\u00e1c b\u01b0\u1edbc th\u1ef1c hi\u1ec7n bao g\u1ed3m hi\u1ec7u chu\u1ea9n thi\u1ebft b\u1ecb b\u1eb1ng dung d\u1ecbch \u0111\u1ec7m, \u0111o \u0111\u1ed9 h\u1ea5p th\u1ee5 c\u1ee7a m\u1eabu v\u00e0 s\u1eed d\u1ee5ng c\u00f4ng th\u1ee9c \u0111\u1ec3 t\u00ednh to\u00e1n n\u1ed3ng \u0111\u1ed9.<\/p>\n\n\n\n<p>\u01afu \u0111i\u1ec3m c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p UV-Vis l\u00e0 nhanh, \u0111\u01a1n gi\u1ea3n, kh\u00f4ng c\u1ea7n thu\u1ed1c th\u1eed \u0111\u1eb7c bi\u1ec7t v\u00e0 c\u00f3 th\u1ec3 \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 tinh s\u1ea1ch c\u1ee7a m\u1eabu. Tuy nhi\u00ean, ph\u01b0\u01a1ng ph\u00e1p n\u00e0y c\u00f3 m\u1ed9t s\u1ed1 h\u1ea1n ch\u1ebf, nghi\u00eam tr\u1ecdng nh\u1ea5t l\u00e0 kh\u00f4ng ph\u00e2n bi\u1ec7t \u0111\u01b0\u1ee3c DNA v\u00e0 RNA, \u0111\u1ed9 nh\u1ea1y kh\u00f4ng \u0111\u1ee7 \u0111\u1ed1i v\u1edbi c\u00e1c m\u1eabu c\u00f3 n\u1ed3ng \u0111\u1ed9 th\u1ea5p, v\u00e0 d\u1ec5 b\u1ecb \u1ea3nh h\u01b0\u1edfng b\u1edfi c\u00e1c ch\u1ea5t g\u00e2y nhi\u1ec5m. Trong c\u00e1c ph\u00e9p \u0111o n\u1ed3ng \u0111\u1ed9 th\u01b0\u1eddng quy sau t\u00e1ch chi\u1ebft plasmid DNA ho\u1eb7c DNA h\u1ec7 gen (genomic DNA), UV-Vis l\u00e0 l\u1ef1a ch\u1ecdn \u01b0u ti\u00ean. Xu h\u01b0\u1edbng hi\u1ec7n nay l\u00e0 ph\u00e1t tri\u1ec3n c\u00e1c m\u00e1y quang ph\u1ed5 th\u1ec3 t\u00edch si\u00eau nh\u1ecf (micro-volume), cho ph\u00e9p \u0111o v\u1edbi ch\u1ec9 1\u20132 \u03bcL m\u1eabu, gi\u00fap n\u00e2ng cao hi\u1ec7u su\u1ea5t l\u00e0m vi\u1ec7c \u0111\u00e1ng k\u1ec3.<\/p>\n\n\n\n<p><em><a href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/kiem-soat-chat-luong-cua-mau-nucleic-acid\/\"><strong>Xem th\u00eam: <\/strong>Ki\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng axit nucleic to\u00e0n di\u1ec7n \u2013 Gi\u1ea3i ph\u00e1p t\u1ed5ng th\u1ec3 cho c\u00e1c v\u1ea5n \u0111\u1ec1 th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed<\/a><\/em><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Do_huynh_quang_Fluorometry\"><\/span>\u0110o hu\u1ef3nh quang (Fluorometry)<span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p><a href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/may-do-quang-pho-ezcube\/\">\u0110o hu\u1ef3nh quang<\/a> cung c\u1ea5p ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic c\u00f3 \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao, \u0111\u1eb7c bi\u1ec7t ph\u00f9 h\u1ee3p cho c\u00e1c m\u1eabu c\u00f3 n\u1ed3ng \u0111\u1ed9 th\u1ea5p. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y s\u1eed d\u1ee5ng <a href=\"https:\/\/www.blue-raybio.com\/en\/category\/product\/EzQuant-Quantification-Assay-Kits\">c\u00e1c thu\u1ed1c nhu\u1ed9m hu\u1ef3nh quang<\/a> li\u00ean k\u1ebft \u0111\u1eb7c hi\u1ec7u v\u1edbi ph\u00e2n t\u1eed axit nucleic, v\u00e0 \u0111o c\u01b0\u1eddng \u0111\u1ed9 hu\u1ef3nh quang \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng ch\u00ednh x\u00e1c axit nucleic. C\u00e1c b\u01b0\u1edbc thao t\u00e1c bao g\u1ed3m chu\u1ea9n b\u1ecb \u0111\u01b0\u1eddng chu\u1ea9n, tr\u1ed9n m\u1eabu v\u1edbi thu\u1ed1c nhu\u1ed9m hu\u1ef3nh quang, \u0111o c\u01b0\u1eddng \u0111\u1ed9 hu\u1ef3nh quang v\u00e0 t\u00ednh to\u00e1n n\u1ed3ng \u0111\u1ed9 d\u1ef1a tr\u00ean \u0111\u01b0\u1eddng chu\u1ea9n.<\/p>\n\n\n\n<p>\u01afu \u0111i\u1ec3m c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p n\u00e0y l\u00e0 \u0111\u1ed9 nh\u1ea1y cao, t\u00ednh \u0111\u1eb7c hi\u1ec7u cao v\u00e0 \u00edt b\u1ecb \u1ea3nh h\u01b0\u1edfng b\u1edfi t\u1ea1p ch\u1ea5t. Tuy nhi\u00ean, c\u1ea7n s\u1eed d\u1ee5ng thu\u1ed1c nhu\u1ed9m v\u00e0 thi\u1ebft b\u1ecb chuy\u00ean d\u1ee5ng (chi ph\u00ed t\u01b0\u01a1ng \u0111\u1ed1i cao), \u0111\u1ed3ng th\u1eddi ph\u1ea3i x\u00e2y d\u1ef1ng \u0111\u01b0\u1eddng chu\u1ea9n. \u0110o hu\u1ef3nh quang l\u00e0 ph\u01b0\u01a1ng ph\u00e1p \u01b0u ti\u00ean trong \u0111\u1ecbnh l\u01b0\u1ee3ng th\u01b0 vi\u1ec7n NGS v\u00e0 c\u00e1c m\u1eabu c\u00f3 n\u1ed3ng \u0111\u1ed9 th\u1ea5p nh\u01b0 cfDNA. Xu h\u01b0\u1edbng m\u1edbi l\u00e0 ph\u00e1t tri\u1ec3n c\u00e1c thu\u1ed1c nhu\u1ed9m hu\u1ef3nh quang th\u1ebf h\u1ec7 m\u1edbi nh\u1eb1m n\u00e2ng cao \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u.<\/p>\n\n\n\n<p><a href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/may-quang-pho-va-may-do-huynh-quang-lua-chon-nao-phu-hop-de-dinh-luong-dna-rna\/\"><em><strong>Xem th\u00eam: <\/strong>M\u00e1y \u0111o quang ph\u1ed5 v\u00e0 m\u00e1y \u0111o hu\u1ef3nh quang: L\u1ef1a ch\u1ecdn n\u00e0o ph\u00f9 h\u1ee3p \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng DNA\/RNA<\/em>?<\/a><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"PCR_dinh_luong_thoi_gian_thuc_qPCR\"><\/span>PCR \u0111\u1ecbnh l\u01b0\u1ee3ng th\u1eddi gian th\u1ef1c (qPCR)<span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p><a href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/may-real-time-pcr\/\">qPCR<\/a> l\u00e0 ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic c\u00f3 \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao, k\u1ebft h\u1ee3p gi\u1eefa khu\u1ebfch \u0111\u1ea1i PCR v\u00e0 ph\u00e1t hi\u1ec7n hu\u1ef3nh quang. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y \u0111\u1ecbnh l\u01b0\u1ee3ng l\u01b0\u1ee3ng axit nucleic ban \u0111\u1ea7u b\u1eb1ng c\u00e1ch theo d\u00f5i t\u00edn hi\u1ec7u hu\u1ef3nh quang ph\u00e1t sinh trong qu\u00e1 tr\u00ecnh PCR theo th\u1eddi gian th\u1ef1c. C\u00e1c b\u01b0\u1edbc th\u1ef1c hi\u1ec7n bao g\u1ed3m thi\u1ebft k\u1ebf primer v\u00e0 probe \u0111\u1eb7c hi\u1ec7u, chu\u1ea9n b\u1ecb thu\u1ed1c th\u1eed ph\u1ea3n \u1ee9ng, ch\u1ea1y ch\u01b0\u01a1ng tr\u00ecnh qPCR, v\u00e0 ph\u00e2n t\u00edch \u0111\u01b0\u1eddng cong khu\u1ebfch \u0111\u1ea1i c\u00f9ng gi\u00e1 tr\u1ecb Ct.<\/p>\n\n\n\n<p>\u01afu \u0111i\u1ec3m c\u1ee7a qPCR l\u00e0 \u0111\u1ed9 nh\u1ea1y c\u1ef1c cao, t\u00ednh \u0111\u1eb7c hi\u1ec7u theo tr\u00ecnh t\u1ef1, v\u00e0 kh\u1ea3 n\u0103ng ph\u00e2n t\u00edch \u0111\u1ecbnh l\u01b0\u1ee3ng v\u00e0 \u0111\u1ecbnh t\u00ednh \u0111\u1ed3ng th\u1eddi. Tuy nhi\u00ean, ph\u01b0\u01a1ng ph\u00e1p n\u00e0y y\u00eau c\u1ea7u thi\u1ebft b\u1ecb v\u00e0 thu\u1ed1c th\u1eed chuy\u00ean d\u1ee5ng, chi ph\u00ed cao, thao t\u00e1c ph\u1ee9c t\u1ea1p v\u00e0 t\u1ed1n nhi\u1ec1u th\u1eddi gian. qPCR l\u00e0 l\u1ef1a ch\u1ecdn \u01b0u ti\u00ean trong ph\u00e1t hi\u1ec7n m\u1ee9c \u0111\u1ed9 bi\u1ec3u hi\u1ec7n gen, t\u1ea3i l\u01b0\u1ee3ng virus, ho\u1eb7c c\u00e1c m\u1eabu c\u00f3 n\u1ed3ng \u0111\u1ed9 axit nucleic c\u1ef1c th\u1ea5p. \u0110\u00e2y c\u0169ng l\u00e0 ph\u01b0\u01a1ng ph\u00e1p \u0111\u00e1ng tin c\u1eady nh\u1ea5t \u0111\u1ed1i v\u1edbi DNA b\u1ecb tho\u00e1i h\u00f3a trong m\u1eabu FFPE.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Dien_di_gel\"><\/span>\u0110i\u1ec7n di gel<span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>\u0110i\u1ec7n di gel l\u00e0 ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic mang t\u00ednh tr\u1ef1c quan, \u0111\u1eb7c bi\u1ec7t ph\u00f9 h\u1ee3p \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 t\u00ednh to\u00e0n v\u1eb9n v\u00e0 k\u00edch th\u01b0\u1edbc ph\u00e2n t\u1eed. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y d\u1ef1a tr\u00ean nguy\u00ean l\u00fd t\u1ed1c \u0111\u1ed9 di chuy\u1ec3n c\u1ee7a axit nucleic trong \u0111i\u1ec7n tr\u01b0\u1eddng t\u1ef7 l\u1ec7 ngh\u1ecbch v\u1edbi kh\u1ed1i l\u01b0\u1ee3ng ph\u00e2n t\u1eed. Ph\u00e2n t\u00edch b\u00e1n \u0111\u1ecbnh l\u01b0\u1ee3ng \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n b\u1eb1ng c\u00e1ch so s\u00e1nh \u0111\u1ed9 s\u00e1ng c\u1ee7a m\u1eabu v\u1edbi c\u00e1c chu\u1ea9n c\u00f3 n\u1ed3ng \u0111\u1ed9 \u0111\u00e3 bi\u1ebft.<\/p>\n\n\n\n<p>\u01afu \u0111i\u1ec3m l\u00e0 c\u00f3 th\u1ec3 \u0111\u1ed3ng th\u1eddi \u0111\u00e1nh gi\u00e1 n\u1ed3ng \u0111\u1ed9, k\u00edch th\u01b0\u1edbc v\u00e0 t\u00ednh to\u00e0n v\u1eb9n c\u1ee7a axit nucleic, cung c\u1ea5p h\u00ecnh \u1ea3nh tr\u1ef1c quan v\u00e0 chi ph\u00ed thi\u1ebft b\u1ecb th\u1ea5p. Tuy nhi\u00ean, ph\u01b0\u01a1ng ph\u00e1p n\u00e0y ch\u1ec9 mang t\u00ednh b\u00e1n \u0111\u1ecbnh l\u01b0\u1ee3ng, \u0111\u1ed9 nh\u1ea1y th\u1ea5p v\u00e0 vi\u1ec7c s\u1eed d\u1ee5ng thu\u1ed1c nhu\u1ed9m \u0111\u1ed9c h\u1ea1i c\u00f3 th\u1ec3 g\u00e2y r\u1ee7i ro an to\u00e0n. \u0110i\u1ec7n di gel th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ki\u1ec3m tra s\u1ea3n ph\u1ea9m PCR, \u0111\u00e1nh gi\u00e1 t\u00ednh to\u00e0n v\u1eb9n c\u1ee7a axit nucleic ho\u1eb7c \u01b0\u1edbc l\u01b0\u1ee3ng s\u01a1 b\u1ed9 n\u1ed3ng \u0111\u1ed9.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Dien_di_mao_quan_Capillary_Electrophoresis\"><\/span>\u0110i\u1ec7n di mao qu\u1ea3n (Capillary Electrophoresis)<span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>\u0110i\u1ec7n di mao qu\u1ea3n l\u00e0 ph\u01b0\u01a1ng ph\u00e1p t\u1ef1 \u0111\u1ed9ng h\u00f3a cao, cho ph\u00e9p ph\u00e2n t\u00edch \u0111\u1ed3ng th\u1eddi n\u1ed3ng \u0111\u1ed9 v\u00e0 k\u00edch th\u01b0\u1edbc axit nucleic. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y k\u1ebft h\u1ee3p c\u00f4ng ngh\u1ec7 t\u00e1ch \u0111i\u1ec7n di v\u00e0 ph\u00e1t hi\u1ec7n hu\u1ef3nh quang. Axit nucleic \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00e1ch v\u1edbi \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao trong c\u00e1c mao qu\u1ea3n nh\u1ecf. C\u00e1c b\u01b0\u1edbc th\u1ef1c hi\u1ec7n bao g\u1ed3m chu\u1ea9n b\u1ecb m\u1eabu, n\u1ea1p m\u1eabu, ph\u00e2n t\u00e1ch t\u1ef1 \u0111\u1ed9ng, ph\u00e1t hi\u1ec7n hu\u1ef3nh quang, v\u00e0 ph\u00e2n t\u00edch d\u1eef li\u1ec7u \u0111\u1ec3 t\u1ea1o b\u00e1o c\u00e1o.<\/p>\n\n\n\n<p>\u01afu \u0111i\u1ec3m bao g\u1ed3m th\u00f4ng l\u01b0\u1ee3ng cao, m\u1ee9c \u0111\u1ed9 t\u1ef1 \u0111\u1ed9ng h\u00f3a cao, v\u00e0 kh\u1ea3 n\u0103ng \u0111o \u0111\u1ed3ng th\u1eddi n\u1ed3ng \u0111\u1ed9 axit nucleic v\u00e0 k\u00edch th\u01b0\u1edbc ph\u00e2n \u0111o\u1ea1n. Tuy nhi\u00ean, thi\u1ebft b\u1ecb n\u00e0y \u0111\u1eaft ti\u1ec1n, quy tr\u00ecnh chu\u1ea9n b\u1ecb m\u1eabu ph\u1ee9c t\u1ea1p v\u00e0 y\u00eau c\u1ea7u ng\u01b0\u1eddi v\u1eadn h\u00e0nh c\u00f3 k\u1ef9 n\u0103ng cao. \u0110i\u1ec7n di mao qu\u1ea3n th\u1ec3 hi\u1ec7n nh\u1eefng \u01b0u \u0111i\u1ec3m \u0111\u1ed9c \u0111\u00e1o trong ki\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng th\u01b0 vi\u1ec7n NGS, ph\u00e2n t\u00edch \u0111o\u1ea1n axit nucleic v\u00e0 s\u00e0ng l\u1ecdc m\u1eabu quy m\u00f4 l\u1edbn.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Cach_lua_chon_phuong_phap_dinh_luong_DNA_phu_hop\"><\/span><strong>C\u00e1ch l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng DNA ph\u00f9 h\u1ee3p<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>Vi\u1ec7c l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic ph\u00f9 h\u1ee3p l\u00e0 y\u1ebfu t\u1ed1 then ch\u1ed1t \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o th\u00e0nh c\u00f4ng th\u00ed nghi\u1ec7m. C\u00e1c y\u1ebfu t\u1ed1 c\u1ea7n xem x\u00e9t bao g\u1ed3m lo\u1ea1i m\u1eabu, n\u1ed3ng \u0111\u1ed9, \u0111\u1ed9 tinh s\u1ea1ch, y\u00eau c\u1ea7u v\u1ec1 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u, c\u00e1c \u1ee9ng d\u1ee5ng ti\u1ebfp theo, c\u0169ng nh\u01b0 th\u1eddi gian v\u00e0 chi ph\u00ed. <a href=\"https:\/\/biogroupvietnam.com\/vi\/danh-muc-san-pham\/sinh-hoc-phan-tu\/\">\u0110o quang ph\u1ed5 UV-Vis<\/a> ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c m\u1eabu c\u00f3 n\u1ed3ng \u0111\u1ed9 cao (&gt;10 ng\/\u03bcL). <a href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/may-do-quang-pho-ezcube\/\">Fluorometry<\/a> ho\u1eb7c <a href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/may-real-time-pcr\/\">qPCR<\/a> ph\u00f9 h\u1ee3p cho m\u1eabu n\u1ed3ng \u0111\u1ed9 th\u1ea5p (&lt;10 ng\/\u03bcL), ho\u1eb7c khi c\u1ea7n ph\u00e2n bi\u1ec7t DNA\/RNA hay ph\u00e1t hi\u1ec7n tr\u00ecnh t\u1ef1 \u0111\u1eb7c hi\u1ec7u. Fluorometry ho\u1eb7c qPCR th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng th\u01b0 vi\u1ec7n NGS, trong \u0111\u00f3 qPCR cho k\u1ebft qu\u1ea3 \u0111\u00e1ng tin c\u1eady nh\u1ea5t \u0111\u1ed1i v\u1edbi c\u00e1c m\u1eabu FFPE.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"So_sanh_cac_phuong_phap_dinh_luong_axit_nucleic\"><\/span><strong>So s\u00e1nh c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><td><strong>Ph\u01b0\u01a1ng ph\u00e1p<\/strong><\/td><td><strong>Kho\u1ea3ng \u0111\u1ed9 nh\u1ea1y<\/strong><\/td><td><strong>\u01afu \u0111i\u1ec3m ch\u00ednh<\/strong><\/td><td><strong>H\u1ea1n ch\u1ebf ch\u00ednh<\/strong><\/td><td><strong>\u1ee8ng d\u1ee5ng ph\u00f9 h\u1ee3p<\/strong><\/td><\/tr><\/thead><tbody><tr><td>UV-Vis Spectrophotometry<\/td><td>2\u20135 ng\/\u03bcL<\/td><td>Nhanh, \u0111\u01a1n gi\u1ea3n, kh\u00f4ng c\u1ea7n thu\u1ed1c th\u1eed \u0111\u1eb7c bi\u1ec7t<\/td><td>Kh\u00f4ng ph\u00e2n bi\u1ec7t DNA\/RNA, d\u1ec5 b\u1ecb nhi\u1ec5m<\/td><td>M\u1eabu tinh s\u1ea1ch n\u1ed3ng \u0111\u1ed9 trung b\u00ecnh \u0111\u1ebfn cao, \u0111o nhanh<\/td><\/tr><tr><td>Fluorometry<\/td><td>0.1\u20130.5 ng\/\u03bcL<\/td><td>\u0110\u1ed9 nh\u1ea1y cao, ph\u00e2n bi\u1ec7t DNA\/RNA<\/td><td>C\u1ea7n \u0111\u01b0\u1eddng chu\u1ea9n, chi ph\u00ed thu\u1ed1c th\u1eed cao<\/td><td>M\u1eabu n\u1ed3ng \u0111\u1ed9 th\u1ea5p, y\u00eau c\u1ea7u \u0111\u1ed9 ch\u00ednh x\u00e1c cao<\/td><\/tr><tr><td>qPCR<\/td><td>&lt;0.1 ng\/\u03bcL<\/td><td>\u0110\u1ed9 nh\u1ea1y c\u1ef1c cao, \u0111\u1eb7c hi\u1ec7u tr\u00ecnh t\u1ef1<\/td><td>Thi\u1ebft b\u1ecb v\u00e0 thu\u1ed1c th\u1eed \u0111\u1eaft, t\u1ed1n nhi\u1ec1u th\u1eddi gian<\/td><td>N\u1ed3ng \u0111\u1ed9 c\u1ef1c th\u1ea5p, ph\u00e1t hi\u1ec7n tr\u00ecnh t\u1ef1 \u0111\u1eb7c hi\u1ec7u<\/td><\/tr><tr><td>Gel Electrophoresis<\/td><td>1\u20135 ng\/band<\/td><td>Quan s\u00e1t k\u00edch th\u01b0\u1edbc DNA v\u00e0 \u0111\u1ed9 to\u00e0n v\u1eb9n<\/td><td>B\u00e1n \u0111\u1ecbnh l\u01b0\u1ee3ng, \u0111\u1ed9 nh\u1ea1y th\u1ea5p<\/td><td>Ki\u1ec3m tra \u0111\u1ed9 to\u00e0n v\u1eb9n, x\u00e1c nh\u1eadn s\u1ea3n ph\u1ea9m PCR<\/td><\/tr><tr><td>Capillary Electrophoresis<\/td><td>0.1\u20130.5 ng\/\u03bcL<\/td><td>Th\u00f4ng l\u01b0\u1ee3ng cao, t\u1ef1 \u0111\u1ed9ng h\u00f3a<\/td><td>Thi\u1ebft b\u1ecb \u0111\u1eaft, chu\u1ea9n b\u1ecb m\u1eabu ph\u1ee9c t\u1ea1p<\/td><td>Ph\u00e2n t\u00edch m\u1eabu quy m\u00f4 l\u1edbn, \u0111\u1ecbnh l\u01b0\u1ee3ng th\u01b0 vi\u1ec7n NGS<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>C\u1ea7n l\u01b0u \u00fd r\u1eb1ng trong c\u00f4ng vi\u1ec7c ph\u00f2ng th\u00ed nghi\u1ec7m h\u00e0ng ng\u00e0y, UV-Vis v\u00e0 fluorometry l\u00e0 hai ph\u01b0\u01a1ng ph\u00e1p \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn nh\u1ea5t do t\u00ednh ti\u1ec7n l\u1ee3i v\u00e0 kh\u1ea3 n\u0103ng \u1ee9ng d\u1ee5ng r\u1ed9ng. Ph\u01b0\u01a1ng ph\u00e1p \u0111o quang ph\u1ed5 UV-Vis ph\u00f9 h\u1ee3p cho c\u00e1c m\u1eabu DNA tinh s\u1ea1ch c\u00f3 n\u1ed3ng \u0111\u1ed9 trung b\u00ecnh \u0111\u1ebfn cao, trong khi fluorometry v\u01b0\u1ee3t tr\u1ed9i trong x\u1eed l\u00fd m\u1eabu n\u1ed3ng \u0111\u1ed9 th\u1ea5p ho\u1eb7c khi c\u1ea7n \u0111\u1ed9 ch\u00ednh x\u00e1c cao.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Cau_hoi_thuong_gap_FAQ\"><\/span><strong>C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p (FAQ)<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><strong>Khi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p kh\u00e1c nhau cho k\u1ebft qu\u1ea3 n\u1ed3ng \u0111\u1ed9 axit nucleic kh\u00e1c bi\u1ec7t \u0111\u00e1ng k\u1ec3, n\u00ean tin v\u00e0o k\u1ebft qu\u1ea3 n\u00e0o?<\/strong><br>Th\u00f4ng th\u01b0\u1eddng, k\u1ebft qu\u1ea3 t\u1eeb fluorometry v\u00e0 qPCR \u0111\u00e1ng tin c\u1eady h\u01a1n, \u0111\u1eb7c bi\u1ec7t \u0111\u1ed1i v\u1edbi m\u1eabu n\u1ed3ng \u0111\u1ed9 th\u1ea5p. Ph\u01b0\u01a1ng ph\u00e1p \u0111o quang ph\u1ed5 UV-Vis c\u00f3 th\u1ec3 \u0111\u00e1nh gi\u00e1 cao h\u01a1n th\u1ef1c t\u1ebf do \u1ea3nh h\u01b0\u1edfng c\u1ee7a t\u1ea1p nhi\u1ec5m. Khuy\u1ebfn ngh\u1ecb ki\u1ec3m ch\u1ee9ng ch\u00e9o c\u00e1c m\u1eabu quan tr\u1ecdng b\u1eb1ng nhi\u1ec1u ph\u01b0\u01a1ng ph\u00e1p.<\/p>\n\n\n\n<p><strong>L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 c\u1ea3i thi\u1ec7n \u0111\u1ed9 ch\u00ednh x\u00e1c khi \u0111\u1ecbnh l\u01b0\u1ee3ng l\u01b0\u1ee3ng nh\u1ecf axit nucleic?<\/strong><br>S\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p \u0111o hu\u1ef3nh quang ho\u1eb7c qPCR, v\u00ec c\u00e1c ph\u01b0\u01a1ng ph\u00e1p n\u00e0y nh\u1ea1y h\u01a1n v\u1edbi n\u1ed3ng \u0111\u1ed9 DNA th\u1ea5p. T\u0103ng s\u1ed1 l\u1ea7n l\u1eb7p l\u1ea1i ph\u00e9p \u0111o, v\u00e0 c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng carrier DNA (DNA mang) \u0111\u1ec3 gi\u1ea3m th\u1ea5t tho\u00e1t m\u1eabu. \u0110\u1ea3m b\u1ea3o s\u1eed d\u1ee5ng \u1ed1ng ly t\u00e2m v\u00e0 \u0111\u1ea7u t\u00edp c\u00f3 \u0111\u1ed9 h\u1ea5p ph\u1ee5 th\u1ea5p.<\/p>\n\n\n\n<p><strong>Ph\u01b0\u01a1ng ph\u00e1p n\u00e0o ph\u00f9 h\u1ee3p nh\u1ea5t \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng th\u01b0 vi\u1ec7n NGS?<\/strong><br>Ph\u01b0\u01a1ng ph\u00e1p qPCR ho\u1eb7c \u0111o hu\u1ef3nh quang l\u00e0 ph\u00f9 h\u1ee3p nh\u1ea5t \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng th\u01b0 vi\u1ec7n NGS. qPCR c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u1eb7c hi\u1ec7u c\u00e1c ph\u00e2n t\u1eed th\u01b0 vi\u1ec7n c\u00f3 adapter, trong khi \u0111o hu\u1ef3nh quang cung c\u1ea5p n\u1ed3ng \u0111\u1ed9 DNA t\u1ed5ng ch\u00ednh x\u00e1c.<\/p>\n\n\n\n<p><strong>Khi l\u00e0m vi\u1ec7c v\u1edbi m\u1eabu FFPE, n\u00ean ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng n\u00e0o?<\/strong><br>\u0110\u1ed1i v\u1edbi m\u1eabu FFPE, qPCR l\u00e0 ph\u01b0\u01a1ng ph\u00e1p \u0111\u00e1ng tin c\u1eady nh\u1ea5t v\u00ec c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n c\u00e1c ph\u00e2n \u0111o\u1ea1n axit nucleic c\u00f2n nguy\u00ean v\u1eb9n v\u00e0 tr\u00e1nh nhi\u1ec5u t\u1eeb DNA b\u1ecb tho\u00e1i h\u00f3a trong qu\u00e1 tr\u00ecnh \u0111o.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Ket_luan\"><\/span><strong>K\u1ebft lu\u1eadn<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>\u0110\u1ecbnh l\u01b0\u1ee3ng axit nucleic l\u00e0 b\u01b0\u1edbc quan tr\u1ecdng trong nghi\u00ean c\u1ee9u sinh h\u1ecdc ph\u00e2n t\u1eed. M\u1ed7i ph\u01b0\u01a1ng ph\u00e1p c\u00f3 nh\u1eefng \u01b0u \u0111i\u1ec3m ri\u00eang v\u00e0 ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c \u1ee9ng d\u1ee5ng kh\u00e1c nhau. Vi\u1ec7c l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p ph\u00f9 h\u1ee3p c\u1ea7n c\u00e2n nh\u1eafc nhi\u1ec1u y\u1ebfu t\u1ed1. Trong th\u1ef1c h\u00e0nh th\u01b0\u1eddng quy, UV-Vis v\u00e0 fluorometry v\u1eabn l\u00e0 c\u00e1c k\u1ef9 thu\u1eadt \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn nh\u1ea5t.<\/p>\n\n\n\n<p>Nhu c\u1ea7u v\u1ec1 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng DNA\/RNA c\u00f3 \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c cao h\u01a1n \u0111\u00e3 th\u00fac \u0111\u1ea9y s\u1ef1 ph\u00e1t tri\u1ec3n nhanh ch\u00f3ng c\u1ee7a c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 ph\u00e2n t\u00edch t\u1ebf b\u00e0o \u0111\u01a1n, sinh thi\u1ebft l\u1ecfng v\u00e0 li\u1ec7u ph\u00e1p gen, t\u1ea1i Vi\u1ec7t Nam c\u0169ng v\u1eady. Trong t\u01b0\u01a1ng lai, ch\u00fang ta c\u00f3 th\u1ec3 ch\u1ee9ng ki\u1ebfn \u200b\u200bnh\u1eefng ph\u01b0\u01a1ng ph\u00e1p ti\u1ebfp c\u1eadn \u0111\u1ed9t ph\u00e1 k\u1ebft h\u1ee3p c\u00f4ng ngh\u1ec7 nano v\u00e0 tr\u00ed tu\u1ec7 nh\u00e2n t\u1ea1o, c\u00f3 kh\u1ea3 n\u0103ng c\u00e1ch m\u1ea1ng h\u00f3a s\u1ef1 hi\u1ec3u bi\u1ebft c\u1ee7a ch\u00fang ta v\u1ec1 \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic. Vi\u1ec7c kh\u00f4ng ng\u1eebng c\u1ea3i ti\u1ebfn c\u00e1c k\u1ef9 thu\u1eadt \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic kh\u00f4ng ch\u1ec9 n\u00e2ng cao \u0111\u1ed9 ch\u00ednh x\u00e1c v\u00e0 hi\u1ec7u qu\u1ea3 th\u00ed nghi\u1ec7m m\u00e0 c\u00f2n m\u1edf ra nh\u1eefng h\u01b0\u1edbng nghi\u00ean c\u1ee9u m\u1edbi cho khoa h\u1ecdc s\u1ef1 s\u1ed1ng.<\/p>\n\n\n\n<p>Biogroup Vietnam chuy\u00ean cung c\u1ea5p c\u00e1c gi\u1ea3i ph\u00e1p c\u00f4ng ngh\u1ec7 sinh h\u1ecdc to\u00e0n di\u1ec7n. L\u00e0 \u0111\u1ed1i t\u00e1c ch\u00ednh th\u1ee9c c\u1ee7a Blue-ray Biotech t\u1ea1i Vi\u1ec7t Nam, ch\u00fang t\u00f4i \u0111\u1ed3ng h\u00e0nh c\u00f9ng c\u00e1c \u0111\u01a1n v\u1ecb nghi\u00ean c\u1ee9u trong vi\u1ec7c x\u00e2y d\u1ef1ng workflow axit nucleic t\u1ed1i \u01b0u, t\u00edch h\u1ee3p linh ho\u1ea1t c\u00e1c c\u00f4ng ngh\u1ec7 \u0111\u1ecbnh l\u01b0\u1ee3ng v\u00e0 ki\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng nh\u1eb1m n\u00e2ng cao \u0111\u1ed9 ch\u00ednh x\u00e1c v\u00e0 kh\u1ea3 n\u0103ng t\u00e1i l\u1eadp c\u1ee7a k\u1ebft qu\u1ea3.<\/p>\n\n\n\n<p><em><a href=\"https:\/\/www.blue-raybio.com\/en\/solution\/Nucleic-Acid-Testing-Solution\"><strong>Xem th\u00eam:<\/strong> Nucleic Acid Testing Solution<\/a><\/em><\/p>\n\n\n\n<p><strong>NGU\u1ed2N B\u00c0I VI\u1ebeT<\/strong><\/p>\n\n\n\n<p>Blue-Ray Biotech. (2024, November 14). <em>Nucleic acid quantification methods: A comprehensive guide<\/em>. <a href=\"https:\/\/www.blue-raybio.com\/en\/blog\/Nucleic-Acid-Quantification-Methods-A-Comprehensive-Guide\">https:\/\/www.blue-raybio.com\/en\/blog\/Nucleic-Acid-Quantification-Methods-A-Comprehensive-Guide<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ph\u01b0\u01a1ng ph\u00e1p n\u00e0o l\u00e0 l\u1ef1a ch\u1ecdn t\u1ed1i \u01b0u \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic? Kh\u00f4ng c\u00f3 c\u00e2u tr\u1ea3 l\u1eddi chung cho m\u1ecdi tr\u01b0\u1eddng h\u1ee3p, b\u1edfi m\u1ed7i k\u1ef9 thu\u1eadt \u0111\u1ec1u c\u00f3 \u0111\u1eb7c \u0111i\u1ec3m ri\u00eang. B\u00e0i vi\u1ebft n\u00e0y s\u1ebd gi\u00fap b\u1ea1n hi\u1ec3u r\u00f5 5 ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic ph\u1ed5 bi\u1ebfn: C\u00f9ng l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p ph\u00f9<\/p>\n<div class=\"klb-readmore post-buttons\"><a class=\"button rounded primary\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/cac-phuong-phap-dinh-luong-axit-nucleic\/\">Read More <i class=\"klbth-icon-right-arrow\"><\/i><\/a><\/div>\n","protected":false},"author":1,"featured_media":10257,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-10256","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>C\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic<\/title>\n<meta name=\"description\" content=\"T\u1ed5ng h\u1ee3p 5 ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic: \u0111o quang ph\u1ed5 UV-Vis, \u0111o hu\u1ef3nh quang, real-time qPCR, \u0111i\u1ec7n di gel v\u00e0 \u0111i\u1ec7n di mao qu\u1ea3n. 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