{"id":4155,"date":"2024-02-25T09:21:28","date_gmt":"2024-02-25T09:21:28","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/2025\/08\/18\/how-to-evaluate-the-morphology-of-hpscs\/"},"modified":"2025-09-29T13:59:53","modified_gmt":"2025-09-29T06:59:53","slug":"cach-danh-gia-hinh-thai-cua-hpscs","status":"publish","type":"post","link":"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/","title":{"rendered":"C\u00e1ch \u0111\u00e1nh gi\u00e1 h\u00ecnh th\u00e1i c\u1ee7a hPSCs"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/#Vi_sao_can_danh_gia_hinh_thai_cua_hPSCs\" >V\u00ec sao c\u1ea7n \u0111\u00e1nh gi\u00e1 h\u00ecnh th\u00e1i c\u1ee7a hPSCs?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/#Hinh_thai_cum_te_bao_khoe_manh_va_cac_bien_the_binh_thuong\" >H\u00ecnh th\u00e1i c\u1ee5m t\u1ebf b\u00e0o kh\u1ecfe m\u1ea1nh v\u00e0 c\u00e1c bi\u1ebfn th\u1ec3 b\u00ecnh th\u01b0\u1eddng<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/#1_Cac_canh_cum_te_bao_%E2%80%9Cco_gai%E2%80%9D_va_dong_goi_long_leo_hon_trong_nhung_ngay_dau_tien_sau_khi_%E2%80%9Cpassage%E2%80%9D\" >1. C\u00e1c c\u1ea1nh c\u1ee5m t\u1ebf b\u00e0o &#8220;c\u00f3 gai&#8221; v\u00e0 \u0111\u00f3ng g\u00f3i l\u1ecfng l\u1ebbo h\u01a1n trong nh\u1eefng ng\u00e0y \u0111\u1ea7u ti\u00ean sau khi \u201cpassage\u201d &nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/#2_Cac_cum_te_bao_khong_doi_xung_vahoac_hop_nhat\" >2.&nbsp;&nbsp; &nbsp;C\u00e1c c\u1ee5m t\u1ebf b\u00e0o kh\u00f4ng \u0111\u1ed1i x\u1ee9ng v\u00e0\/ho\u1eb7c h\u1ee3p nh\u1ea5t<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/#Cac_bien_the_ve_hinh_thai_cho_thay_chat_luong_te_bao_kem\" >C\u00e1c bi\u1ebfn th\u1ec3 v\u1ec1 h\u00ecnh th\u00e1i cho th\u1ea5y ch\u1ea5t l\u01b0\u1ee3ng t\u1ebf b\u00e0o k\u00e9m<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/#Cac_khu_vuc_biet_hoa\" >C\u00e1c khu v\u1ef1c bi\u1ec7t h\u00f3a<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/#Duoi_day_la_mot_so_dac_diem_cua_hinh_thai_cum_te_bao_kem_cho_thay_chat_luong_te_bao_giam\" >D\u01b0\u1edbi \u0111\u00e2y l\u00e0 m\u1ed9t s\u1ed1 \u0111\u1eb7c \u0111i\u1ec3m c\u1ee7a h\u00ecnh th\u00e1i c\u1ee5m t\u1ebf b\u00e0o k\u00e9m cho th\u1ea5y ch\u1ea5t l\u01b0\u1ee3ng t\u1ebf b\u00e0o gi\u1ea3m<\/a><\/li><\/ul><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"Vi_sao_can_danh_gia_hinh_thai_cua_hPSCs\"><\/span><strong>V\u00ec sao c\u1ea7n \u0111\u00e1nh gi\u00e1 h\u00ecnh th\u00e1i c\u1ee7a hPSCs?<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Vi\u1ec7c nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o g\u1ed1c \u0111a n\u0103ng c\u1ee7a con ng\u01b0\u1eddi (hPSCs) hi\u1ec7n c\u00f3 m\u1ed9t s\u1ed1 th\u00e1ch th\u1ee9c h\u01a1n khi so s\u00e1nh v\u1edbi vi\u1ec7c nu\u00f4i c\u1ea5y c\u00e1c t\u1ebf b\u00e0o k\u1ebft d\u00ednh kh\u00e1c. C\u00e1c k\u1ef9 thu\u1eadt theo d\u00f5i v\u00e0 x\u1eed l\u00fd c\u1ea9n th\u1eadn l\u00e0 r\u1ea5t quan tr\u1ecdng \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o duy tr\u00ec v\u00e0 m\u1edf r\u1ed9ng c\u00e1c t\u1ebf b\u00e0o kh\u1ecfe m\u1ea1nh \u1edf tr\u1ea1ng th\u00e1i kh\u00f4ng ph\u00e2n h\u00f3a trong th\u1eddi gian d\u00e0i.<br \/>M\u1eb7c d\u00f9 c\u00e1c \u0111\u00e1nh gi\u00e1 tr\u1ef1c quan c\u00f3 th\u1ec3 mang t\u00ednh ch\u1ee7 quan, nh\u01b0ng nh\u1eefng thay \u0111\u1ed5i c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y \u0111\u01b0\u1ee3c gi\u00fap ch\u00fang ta \u0111\u00e1nh gi\u00e1 ch\u1ea5t l\u01b0\u1ee3ng hPSC. Vi\u1ec7c nu\u00f4i c\u1ea5y n\u00ean \u0111\u01b0\u1ee3c ki\u1ec3m tra h\u00e0ng ng\u00e0y b\u1eb1ng k\u00ednh hi\u1ec3n vi v\u1edbi \u00e1nh s\u00e1ng c\u00f3 \u0111\u1ed9 t\u01b0\u01a1ng ph\u1ea3n \u0111\u1ec3 theo d\u00f5i ch\u1ea5t l\u01b0\u1ee3ng c\u1ee7a c\u00e1c t\u1ebf b\u00e0o, c\u0169ng nh\u01b0 \u0111\u1ec3 ki\u1ec3m tra xem ch\u00fang \u0111\u00e3 s\u1eb5n s\u00e0ng \u0111\u1ec3 \u201cpass cell\u201d ch\u01b0a.<br \/>C\u1ea7n l\u01b0u \u00fd r\u1eb1ng kh\u00f4ng ph\u1ea3i t\u1ea5t c\u1ea3 nh\u1eefng thay \u0111\u1ed5i v\u1ec1 ngo\u1ea1i h\u00ecnh \u0111\u1ec1u th\u1ec3 hi\u1ec7n s\u1ef1 gi\u1ea3m s\u00fat v\u1ec1 ch\u1ea5t l\u01b0\u1ee3ng. Vi\u1ec7c c\u00f3 c\u00e1c bi\u1ebfn th\u1ec3 do m\u1ed9t s\u1ed1 y\u1ebfu t\u1ed1 l\u00e0 \u0111i\u1ec1u b\u00ecnh th\u01b0\u1eddng. Sau \u0111\u00e2y l\u00e0 c\u00e1c m\u1eb9o k\u1ef9 thu\u1eadt m\u00f4 t\u1ea3 h\u00ecnh th\u00e1i d\u1ef1 ki\u1ebfn c\u1ee7a c\u00e1c t\u1ebf b\u00e0o ch\u1ea5t l\u01b0\u1ee3ng cao, m\u1ed9t s\u1ed1 bi\u1ebfn th\u1ec3 b\u00ecnh th\u01b0\u1eddng ph\u1ed5 bi\u1ebfn v\u00e0 c\u00e1c bi\u1ebfn th\u1ec3 kh\u00e1c c\u00f3 th\u1ec3 cho th\u1ea5y ch\u1ea5t l\u01b0\u1ee3ng t\u1ebf b\u00e0o k\u00e9m.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Hinh_thai_cum_te_bao_khoe_manh_va_cac_bien_the_binh_thuong\"><\/span><strong>H\u00ecnh th\u00e1i c\u1ee5m t\u1ebf b\u00e0o kh\u1ecfe m\u1ea1nh v\u00e0 c\u00e1c bi\u1ebfn th\u1ec3 b\u00ecnh th\u01b0\u1eddng<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Vi\u1ec7c \u0111\u00e1nh gi\u00e1 ch\u1ea5t l\u01b0\u1ee3ng hPSC c\u00f3 th\u1ec3 kh\u00f3 kh\u0103n trong v\u00e0i ng\u00e0y \u0111\u1ea7u sau khi \u201cpassaging\u201d v\u00e0 tr\u1edf n\u00ean d\u1ec5 d\u00e0ng h\u01a1n khi c\u00e1c c\u1ee5m t\u1ebf b\u00e0o tr\u01b0\u1edfng th\u00e0nh. \u0110i\u1ec1u n\u00e0y th\u01b0\u1eddng x\u1ea3y ra kho\u1ea3ng 48 gi\u1edd tr\u01b0\u1edbc th\u1eddi gian \u201cpassaging\u201d t\u1ed1i \u01b0u. D\u01b0\u1edbi \u0111\u1ed9 ph\u00f3ng \u0111\u1ea1i th\u1ea5p, hPSC ch\u1ea5t l\u01b0\u1ee3ng cao v\u00e0 kh\u1ecfe m\u1ea1nh ph\u1ea3i th\u1ec3 hi\u1ec7n c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m sau:<\/p>\n<ul>\n<li>C\u1ee5m t\u1ebf b\u00e0o t\u01b0\u01a1ng \u0111\u1ed1i tr\u00f2n<\/li>\n<li>T\u1ebf b\u00e0o \u0111\u01b0\u1ee3c \u0111\u00f3ng g\u00f3i ch\u1eb7t ch\u1ebd v\u1edbi t\u1ef7 l\u1ec7 nh\u00e2n: t\u1ebf b\u00e0o ch\u1ea5t cao, n\u01a1i nh\u00e2n th\u1ef1c t\u1ebf l\u00e0 n\u01a1i c\u01b0 tr\u00fa c\u1ee7a to\u00e0n b\u1ed9 t\u1ebf b\u00e0o<\/li>\n<li>H\u1ea1t nh\u00e2n n\u1ed5i b\u1eadt<\/li>\n<li>C\u00e1c trung t\u00e2m c\u1ee5m t\u1ebf b\u00e0o tr\u1edf n\u00ean r\u1ea5t d\u00e0y \u0111\u1eb7c v\u00e0 c\u00f3 th\u1ec3 xu\u1ea5t hi\u1ec7n \u1edf d\u1ea1ng pha s\u00e1ng h\u01a1n khi quan s\u00e1t d\u01b0\u1edbi k\u00ednh hi\u1ec3n vi c\u00f3 \u0111\u1ed9 t\u01b0\u01a1ng ph\u1ea3n<\/li>\n<\/ul>\n<p style=\"text-align:center\">&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp; &nbsp;<img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/assets\/uploads\/image\/585cf12eb0d1478f1ec0.jpg\" alt=\"H\u00ecnh th\u00e1i c\u1ee7a hPSCs\" width=\"487\" height=\"335\">&nbsp;<\/p>\n<p style=\"text-align:center\">H\u00ecnh 1. C\u00e1c c\u1ee5m t\u1ebf b\u00e0o hPSC ch\u1ea5t l\u01b0\u1ee3ng cao g\u1ea7n th\u1eddi gian \u201cpassaging\u201d t\u1ed1i \u01b0u<\/p>\n<p>M\u1ed9t s\u1ed1 y\u1ebfu t\u1ed1 c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn h\u00ecnh th\u1ee9c tr\u1ef1c quan c\u1ee7a c\u1ee5m t\u1ebf b\u00e0o bao g\u1ed3m s\u1ed1 ng\u00e0y k\u1ec3 t\u1eeb l\u1ea7n \u201cpassaging\u201d ho\u1eb7c l\u1ea7n th\u00eam m\u00f4i tr\u01b0\u1eddng cu\u1ed1i c\u00f9ng, d\u00f2ng t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng, m\u00f4i tr\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng, ph\u01b0\u01a1ng ph\u00e1p \u201cpassaging\u201d. D\u01b0\u1edbi \u0111\u00e2y l\u00e0 m\u1ed9t s\u1ed1 bi\u1ebfn th\u1ec3 m\u00e0 b\u1ea1n c\u00f3 th\u1ec3 nh\u1eadn th\u1ea5y, \u0111\u01b0\u1ee3c coi l\u00e0 b\u00ecnh th\u01b0\u1eddng v\u00e0 kh\u00f4ng nh\u1ea5t thi\u1ebft l\u00e0 d\u1ea5u hi\u1ec7u c\u1ee7a ch\u1ea5t l\u01b0\u1ee3ng t\u1ebf b\u00e0o k\u00e9m:<\/p>\n<h3><span class=\"ez-toc-section\" id=\"1_Cac_canh_cum_te_bao_%E2%80%9Cco_gai%E2%80%9D_va_dong_goi_long_leo_hon_trong_nhung_ngay_dau_tien_sau_khi_%E2%80%9Cpassage%E2%80%9D\"><\/span><strong>1. C\u00e1c c\u1ea1nh c\u1ee5m t\u1ebf b\u00e0o &#8220;c\u00f3 gai&#8221; v\u00e0 \u0111\u00f3ng g\u00f3i l\u1ecfng l\u1ebbo h\u01a1n trong nh\u1eefng ng\u00e0y \u0111\u1ea7u ti\u00ean sau khi \u201cpassage\u201d &nbsp;<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>Trong t\u1ed1i \u0111a 4 ng\u00e0y sau khi c\u1ea5y, c\u00e1c c\u1ee5m t\u1ebf b\u00e0o c\u00f3 th\u1ec3 bi\u1ec3u hi\u1ec7n s\u1ef1 \u0111\u00f3ng g\u00f3i l\u1ecfng l\u1ebbo h\u01a1n khi c\u00e1c c\u1ee5m t\u1ebf b\u00e0o lan r\u1ed9ng ra v\u00e0 h\u00ecnh th\u00e0nh. T\u1ea1i th\u1eddi \u0111i\u1ec3m n\u00e0y, c\u00e1c c\u1ea1nh c\u1ee7a c\u1ee5m t\u1ebf b\u00e0o c\u00f3 nhi\u1ec1u gai nh\u1ecdn kh\u00f4ng ph\u1ea3i l\u00e0 nguy\u00ean nh\u00e2n \u0111\u00e1ng lo ng\u1ea1i. M\u1eadt \u0111\u1ed9 v\u00e0 \u0111\u1ed9 ch\u1eafc c\u1ee7a c\u1ee5m t\u1ebf b\u00e0o t\u0103ng nhanh sau m\u1ed1c th\u1eddi gian n\u00e0y v\u00e0 h\u00ecnh th\u00e1i thay \u0111\u1ed5i \u0111\u00e1ng k\u1ec3 trong 1 &#8211; 2 ng\u00e0y cu\u1ed1i c\u00f9ng tr\u01b0\u1edbc khi \u201cpassaging\u201d.<\/p>\n<p style=\"text-align:center\">&nbsp; <img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/assets\/uploads\/image\/z2681291153154_96a92fc27bae71282d523b04fd6adf62.jpg\" alt=\"H\u00ecnh th\u00e1i c\u1ee7a hPSCs\" width=\"279\" height=\"200\"><\/p>\n<p style=\"text-align:center\">H\u00ecnh 2. T\u1ebf b\u00e0o g\u1ed1c ph\u00f4i ng\u01b0\u1eddi (ES) (H9) Hai ng\u00e0y sau khi \u201cpassaging\u201d v\u1edbi c\u00e1c c\u1ea1nh c\u1ee5m t\u1ebf b\u00e0o c\u00f3 gai (M\u0169i t\u00ean m\u00e0u cam)<\/p>\n<h3><span class=\"ez-toc-section\" id=\"2_Cac_cum_te_bao_khong_doi_xung_vahoac_hop_nhat\"><\/span><strong>2.&nbsp;&nbsp; &nbsp;C\u00e1c c\u1ee5m t\u1ebf b\u00e0o kh\u00f4ng \u0111\u1ed1i x\u1ee9ng v\u00e0\/ho\u1eb7c h\u1ee3p nh\u1ea5t<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>C\u00e1c c\u1ee5m t\u1ebf b\u00e0o c\u00f3 th\u1ec3 h\u01a1i kh\u00f4ng \u0111\u1ed1i x\u1ee9ng v\u00e0 h\u1ee3p nh\u1ea5t, \u0111\u1eb7c bi\u1ec7t l\u00e0 v\u00e0o th\u1eddi \u0111i\u1ec3m \u201cpassaging\u201d. \u0110\u00e2y th\u01b0\u1eddng kh\u00f4ng ph\u1ea3i l\u00e0 nguy\u00ean nh\u00e2n \u0111\u00e1ng lo ng\u1ea1i. C\u00e1c c\u1ee5m t\u1ebf b\u00e0o h\u1ee3p nh\u1ea5t c\u0169ng c\u00f3 th\u1ec3 x\u1ea3y ra khi c\u00e1c t\u1eadp h\u1ee3p \u0111\u01b0\u1ee3c c\u1ea5y \u1edf m\u1eadt \u0111\u1ed9 th\u1ea5p v\u00e0 kh\u00f4ng \u0111\u01b0\u1ee3c ph\u00e2n t\u00e1n t\u1ed1t. M\u1ed9t l\u1ea7n n\u1eefa, \u0111\u00e2y th\u01b0\u1eddng kh\u00f4ng ph\u1ea3i l\u00e0 nguy\u00ean nh\u00e2n \u0111\u00e1ng lo ng\u1ea1i. S\u1ef1 \u0111\u1ed1i x\u1ee9ng c\u1ee7a c\u00e1c c\u1ee5m t\u1ebf b\u00e0o c\u00f3 th\u1ec3 kh\u00e1c nhau kh\u00f4ng ch\u1ec9 gi\u1eefa c\u00e1c d\u00f2ng t\u1ebf b\u00e0o m\u00e0 c\u00f2n gi\u1eefa c\u00e1c n\u1ec1n, v\u1edbi c\u00e1c hPSC \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y trong Vitronectin XF \u2122 (STEMCELL Technology) th\u01b0\u1eddng tr\u00f4ng nh\u1ecf g\u1ecdn v\u00e0 \u0111\u1ed1i x\u1ee9ng h\u01a1n. &nbsp;<\/p>\n<p style=\"text-align:center\"><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/assets\/uploads\/image\/z2681293188993_31e57322f95dafec80afdf12ca429827.jpg\" alt=\"H\u00ecnh th\u00e1i c\u1ee7a hPSCs\" width=\"454\" height=\"158\"><\/p>\n<p style=\"text-align:center\">H\u00ecnh 3. (A) T\u1ebf b\u00e0o g\u1ed1c \u0111a n\u0103ng c\u1ea3m \u1ee9ng (iPS) ng\u01b0\u1eddi, t\u1ebf b\u00e0o WLS-1C nu\u00f4i c\u1ea5y trong Vitronectin XF \u2122 v\u00e0 (B) T\u1ebf b\u00e0o ES c\u1ee7a ng\u01b0\u1eddi (H9) tr\u00ean Corning\u00ae Matrigel\u00ae<\/p>\n<p>Ph\u1ea7n ti\u1ebfp theo bao g\u1ed3m m\u1ed9t s\u1ed1 v\u00ed d\u1ee5 v\u1ec1 h\u00ecnh th\u00e1i c\u1ea7n \u0111\u01b0\u1ee3c theo d\u00f5i th\u00eam, cho th\u1ea5y ch\u1ea5t l\u01b0\u1ee3ng t\u1ebf b\u00e0o gi\u1ea3m.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Cac_bien_the_ve_hinh_thai_cho_thay_chat_luong_te_bao_kem\"><\/span><strong>C\u00e1c bi\u1ebfn th\u1ec3 v\u1ec1 h\u00ecnh th\u00e1i cho th\u1ea5y ch\u1ea5t l\u01b0\u1ee3ng t\u1ebf b\u00e0o k\u00e9m<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<h3><span class=\"ez-toc-section\" id=\"Cac_khu_vuc_biet_hoa\"><\/span><strong>C\u00e1c khu v\u1ef1c bi\u1ec7t h\u00f3a<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>C\u00e1c c\u1ee5m t\u1ebf b\u00e0o hPSC kh\u1ecfe m\u1ea1nh bi\u1ec3u hi\u1ec7n m\u1ed9t l\u01b0\u1ee3ng th\u1ea5p (5-10%) s\u1ef1 bi\u1ec7t h\u00f3a t\u1ef1 ph\u00e1t l\u00e0 \u0111i\u1ec1u b\u00ecnh th\u01b0\u1eddng. Tuy nhi\u00ean, s\u1ef1 gia t\u0103ng c\u00e1c khu v\u1ef1c bi\u1ec7t h\u00f3a l\u00e0 d\u1ea5u hi\u1ec7u c\u1ee7a c\u00e1c hPSC k\u00e9m ch\u1ea5t l\u01b0\u1ee3ng. \u0110\u00e1ng ch\u00fa \u00fd, c\u00e1c d\u1ea5u hi\u1ec7u c\u1ee7a s\u1ef1 bi\u1ec7t h\u00f3a c\u00f3 th\u1ec3 xu\u1ea5t hi\u1ec7n tr\u01b0\u1edbc khi bi\u1ec3u hi\u1ec7n d\u1ea5u hi\u1ec7u (marker) gi\u1ea3m.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Duoi_day_la_mot_so_dac_diem_cua_hinh_thai_cum_te_bao_kem_cho_thay_chat_luong_te_bao_giam\"><\/span><strong>D\u01b0\u1edbi \u0111\u00e2y l\u00e0 m\u1ed9t s\u1ed1 \u0111\u1eb7c \u0111i\u1ec3m c\u1ee7a h\u00ecnh th\u00e1i c\u1ee5m t\u1ebf b\u00e0o k\u00e9m cho th\u1ea5y ch\u1ea5t l\u01b0\u1ee3ng t\u1ebf b\u00e0o gi\u1ea3m<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li>S\u1ef1 bi\u1ec7t h\u00f3a t\u1ef1 ph\u00e1t t\u0103ng (&gt; 10%)<\/li>\n<li>M\u1ea5t t\u00ednh \u0111\u1ed3ng nh\u1ea5t<\/li>\n<li>\u0110\u1ed9 s\u00e1ng ho\u1eb7c nhi\u1ec1u l\u1edbp xu\u1ea5t hi\u1ec7n &#8220;l\u1ed1m \u0111\u1ed1m&#8221;, r\u1eddi r\u1ea1c v\u00e0 kh\u00f4ng \u1edf trung t\u00e2m c\u1ee7a c\u1ee5m t\u1ebf b\u00e0o<\/li>\n<li>M\u1ea5t t\u00ednh to\u00e0n v\u1eb9n c\u1ee7a c\u1ee5m t\u1ebf b\u00e0o<\/li>\n<li>\u0110\u00f3ng g\u00f3i t\u1ebf b\u00e0o l\u1ecfng l\u1ebbo<\/li>\n<\/ul>\n<p style=\"text-align:center\">&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp;<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/assets\/uploads\/image\/z2681297422204_bc5f45b940ccf19e2c042347deedbbc5.jpg\" alt=\"H\u00ecnh th\u00e1i c\u1ee7a hPSCs\" width=\"488\" height=\"170\"><\/p>\n<p style=\"text-align:center\">H\u00ecnh 4. (A) C\u00e1c khu v\u1ef1c bi\u1ec7t h\u00f3a t\u1ef1 ph\u00e1t v\u00e0 (B) M\u1ea5t t\u00ednh to\u00e0n v\u1eb9n c\u1ee7a c\u1ee5m t\u1ebf b\u00e0o v\u00e0 c\u00e1c t\u1ebf b\u00e0o b\u1ecb bi\u1ec7t h\u00f3a (M\u0169i t\u00ean m\u00e0u cam) trong c\u00e1c c\u1ee5m t\u1ebf b\u00e0o STiPS-M001<\/p>\n<p style=\"text-align:center\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/assets\/uploads\/image\/z2681299510040_befb3bc217be7d276b6185efbfbe6fc7.jpg\" alt=\"H\u00ecnh th\u00e1u hPSCs\" width=\"484\" height=\"168\"><\/p>\n<p style=\"text-align:center\">H\u00ecnh 5. (A) T\u1ebf b\u00e0o \u0111\u01b0\u1ee3c \u0111\u00f3ng g\u00f3i ch\u1eb7t ch\u1ebd so v\u1edbi (B) T\u1ebf b\u00e0o \u0111\u01b0\u1ee3c \u0111\u00f3ng g\u00f3i l\u1ecfng l\u1ebbo v\u1edbi kho\u1ea3ng c\u00e1ch pha s\u00e1ng c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y gi\u1eefa c\u00e1c t\u1ebf b\u00e0o trong c\u1ee5m t\u1ebf b\u00e0o hPSC (M\u0169i t\u00ean m\u00e0u cam)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>V\u00ec sao c\u1ea7n \u0111\u00e1nh gi\u00e1 h\u00ecnh th\u00e1i c\u1ee7a hPSCs? Vi\u1ec7c nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o g\u1ed1c \u0111a n\u0103ng c\u1ee7a con ng\u01b0\u1eddi (hPSCs) hi\u1ec7n c\u00f3 m\u1ed9t s\u1ed1 th\u00e1ch th\u1ee9c h\u01a1n khi so s\u00e1nh v\u1edbi vi\u1ec7c nu\u00f4i c\u1ea5y c\u00e1c t\u1ebf b\u00e0o k\u1ebft d\u00ednh kh\u00e1c. C\u00e1c k\u1ef9 thu\u1eadt theo d\u00f5i v\u00e0 x\u1eed l\u00fd c\u1ea9n th\u1eadn l\u00e0 r\u1ea5t quan tr\u1ecdng<\/p>\n<div class=\"klb-readmore post-buttons\"><a class=\"button rounded primary\" href=\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/\">Read More <i class=\"klbth-icon-right-arrow\"><\/i><\/a><\/div>\n","protected":false},"author":1,"featured_media":5629,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[17],"tags":[],"class_list":["post-4155","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cong-nghe-san-pham-moi"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>C\u00e1ch \u0111\u00e1nh gi\u00e1 h\u00ecnh th\u00e1i c\u1ee7a hPSCs<\/title>\n<meta name=\"description\" content=\"Vi\u1ec7c nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o g\u1ed1c \u0111a n\u0103ng c\u1ee7a con ng\u01b0\u1eddi (hPSCs) hi\u1ec7n c\u00f3 m\u1ed9t s\u1ed1 th\u00e1ch th\u1ee9c h\u01a1n khi so s\u00e1nh v\u1edbi vi\u1ec7c nu\u00f4i c\u1ea5y c\u00e1c t\u1ebf b\u00e0o k\u1ebft d\u00ednh kh\u00e1c.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/\" \/>\n<meta property=\"og:locale\" content=\"vi_VN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"C\u00e1ch \u0111\u00e1nh gi\u00e1 h\u00ecnh th\u00e1i c\u1ee7a hPSCs\" \/>\n<meta property=\"og:description\" content=\"Vi\u1ec7c nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o g\u1ed1c \u0111a n\u0103ng c\u1ee7a con ng\u01b0\u1eddi (hPSCs) hi\u1ec7n c\u00f3 m\u1ed9t s\u1ed1 th\u00e1ch th\u1ee9c h\u01a1n khi so s\u00e1nh v\u1edbi vi\u1ec7c nu\u00f4i c\u1ea5y c\u00e1c t\u1ebf b\u00e0o k\u1ebft d\u00ednh kh\u00e1c.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/\" \/>\n<meta property=\"og:site_name\" content=\"Biogroup Vietnam\" \/>\n<meta property=\"article:published_time\" content=\"2024-02-25T09:21:28+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-09-29T06:59:53+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/GDIUXJIHsqryd9m5WSkIRdB05oN4w5PPOJwvWBJe.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"603\" \/>\n\t<meta property=\"og:image:height\" content=\"344\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"\u0110\u01b0\u1ee3c vi\u1ebft b\u1edfi\" \/>\n\t<meta name=\"twitter:data1\" content=\"admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u01af\u1edbc t\u00ednh th\u1eddi gian \u0111\u1ecdc\" \/>\n\t<meta name=\"twitter:data2\" content=\"8 ph\u00fat\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/\"},\"author\":{\"name\":\"admin\",\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/#\/schema\/person\/78a1c640012e6e7bc2bdf0d0fbffea95\"},\"headline\":\"C\u00e1ch \u0111\u00e1nh gi\u00e1 h\u00ecnh th\u00e1i c\u1ee7a hPSCs\",\"datePublished\":\"2024-02-25T09:21:28+00:00\",\"dateModified\":\"2025-09-29T06:59:53+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/\"},\"wordCount\":1647,\"image\":{\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/cong-nghe-san-pham-moi\/cach-danh-gia-hinh-thai-cua-hpscs\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/GDIUXJIHsqryd9m5WSkIRdB05oN4w5PPOJwvWBJe.jpg\",\"articleSection\":[\"S\u1ea3n Ph\u1ea9m &amp; 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