{"id":4216,"date":"2024-02-25T10:12:12","date_gmt":"2024-02-25T10:12:12","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/2025\/08\/18\/isolation-and-purification-of-evs-with-the-easysep-human-extracellular-vesicle-positive-selection-kit\/"},"modified":"2025-12-03T13:42:51","modified_gmt":"2025-12-03T06:42:51","slug":"phan-lap-va-tinh-sach-ev-voi-bo-dung-cu-chon-loc-duong-tinh-tui-ngoai-bao-easysep-cua-nguoi","status":"publish","type":"post","link":"https:\/\/biogroupvietnam.com\/vi\/blog\/phan-lap-va-tinh-sach-ev-voi-bo-dung-cu-chon-loc-duong-tinh-tui-ngoai-bao-easysep-cua-nguoi\/","title":{"rendered":"Ph\u00e2n l\u1eadp &amp; tinh s\u1ea1ch EV v\u1edbi b\u1ed9 d\u1ee5ng c\u1ee5 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 c\u1ee7a ng\u01b0\u1eddi"},"content":{"rendered":"<p>C\u00e1c t\u00fai ngo\u1ea1i b\u00e0o (EV), bao g\u1ed3m exosome v\u00e0 microvesicles, \u0111\u01b0\u1ee3c bao quanh b\u1edfi m\u00e0ng k\u00e9p lipid c\u00f3 c\u1ea5u tr\u00fac t\u1ef1 nhi\u00ean, \u0111\u01b0\u1ee3c gi\u1ea3i ph\u00f3ng t\u1eeb t\u1ebf b\u00e0o. EV tham gia v\u00e0o ch\u1ee9c n\u0103ng giao ti\u1ebfp gi\u1eefa c\u00e1c t\u1ebf b\u00e0o trong \u0111i\u1ec1u ki\u1ec7n c\u00e2n b\u1eb1ng n\u1ed9i m\u00f4i v\u00e0 b\u1ec7nh l\u00fd, th\u01b0\u1eddng \u0111\u01b0\u1ee3c \u0111\u1eb7c tr\u01b0ng b\u1edfi s\u1ef1 bi\u1ec3u hi\u1ec7n c\u1ee7a protein tetraspanin CD9, CD63 v\u00e0 CD81. Lumen c\u1ee7a EV ch\u1ee9a protein, RNA\/DNA, cytokine ho\u1eb7c lipid ph\u1ea3n \u00e1nh tr\u1ea1ng th\u00e1i c\u1ee7a t\u1ebf b\u00e0o g\u1ed1c. T\u1eeb c\u00e1c nghi\u00ean c\u1ee9u c\u01a1 b\u1ea3n v\u1ec1 EV, hi\u1ec7n nay m\u1ed1i quan t\u00e2m ng\u00e0y c\u00e0ng t\u0103ng trong vi\u1ec7c nghi\u00ean c\u1ee9u th\u00e0nh ph\u1ea7n c\u1ee7a EV \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh c\u00e1c d\u1ea5u \u1ea5n sinh h\u1ecdc ch\u1ea9n \u0111o\u00e1n ho\u1eb7c ti\u00ean l\u01b0\u1ee3ng b\u1ec7nh c\u0169ng nh\u01b0 \u0111\u00e1nh gi\u00e1 ti\u1ec1m n\u0103ng \u0111i\u1ec1u tr\u1ecb c\u1ee7a EV.<\/p>\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/phan-lap-va-tinh-sach-ev-voi-bo-dung-cu-chon-loc-duong-tinh-tui-ngoai-bao-easysep-cua-nguoi\/#Tai_sao_nen_su_dung_Bo_dung_cu_chon_loc_duong_tinh_tui_ngoai_bao_EasySep%E2%84%A2_o_nguoi\" >T\u1ea1i sao n\u00ean s\u1eed d\u1ee5ng B\u1ed9 d\u1ee5ng c\u1ee5 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 \u1edf ng\u01b0\u1eddi?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/phan-lap-va-tinh-sach-ev-voi-bo-dung-cu-chon-loc-duong-tinh-tui-ngoai-bao-easysep-cua-nguoi\/#Bo_dung_cu_chon_loc_duong_tinh_tui_ngoai_bao_EasySep%E2%84%A2_o_nguoi\" >B\u1ed9 d\u1ee5ng c\u1ee5 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 \u1edf ng\u01b0\u1eddi<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/phan-lap-va-tinh-sach-ev-voi-bo-dung-cu-chon-loc-duong-tinh-tui-ngoai-bao-easysep-cua-nguoi\/#Quy_trinh_thuc_hien\" >Quy tr\u00ecnh th\u1ef1c hi\u1ec7n<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/phan-lap-va-tinh-sach-ev-voi-bo-dung-cu-chon-loc-duong-tinh-tui-ngoai-bao-easysep-cua-nguoi\/#Thong_tin_san_pham\" >Th\u00f4ng tin s\u1ea3n ph\u1ea9m<\/a><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"Tai_sao_nen_su_dung_Bo_dung_cu_chon_loc_duong_tinh_tui_ngoai_bao_EasySep%E2%84%A2_o_nguoi\"><\/span><strong>T\u1ea1i sao n\u00ean s\u1eed d\u1ee5ng B\u1ed9 d\u1ee5ng c\u1ee5 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 \u1edf ng\u01b0\u1eddi?<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<ul>\n<li>Ph\u00e2n l\u1eadp EV c\u1ee7a ng\u01b0\u1eddi trong \u00edt nh\u1ea5t 30 ph\u00fat m\u00e0 kh\u00f4ng c\u1ea7n s\u1eed d\u1ee5ng c\u1ed9t<\/li>\n<li>Tr\u00e1nh nhu c\u1ea7u si\u00eau ly t\u00e2m v\u00e0 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n l\u1eadp EV t\u1ed1n th\u1eddi gian kh\u00e1c.<\/li>\n<li>Hi\u1ec7u qu\u1ea3 cao h\u01a1n so v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n l\u1eadp EV d\u1ef1a tr\u00ean si\u00eau ly t\u00e2m<\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"Bo_dung_cu_chon_loc_duong_tinh_tui_ngoai_bao_EasySep%E2%84%A2_o_nguoi\"><\/span><strong>B\u1ed9 d\u1ee5ng c\u1ee5 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 \u1edf ng\u01b0\u1eddi<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>C\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n l\u1eadp EV truy\u1ec1n th\u1ed1ng d\u1ef1a tr\u00ean gradient t\u1ec9 tr\u1ecdng y\u00eau c\u1ea7u ph\u1ea3i s\u1eed d\u1ee5ng m\u00e1y si\u00eau ly t\u00e2m v\u00e0 quy tr\u00ecnh t\u1ed1n th\u1eddi gian.<\/p>\n<p>B\u1ed9 d\u1ee5ng c\u1ee5 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 \u1edf ng\u01b0\u1eddi nh\u1eafm v\u00e0o vi\u1ec7c ph\u00e2n l\u1eadp EV s\u1eed d\u1ee5ng ph\u1ee9c h\u1ee3p kh\u00e1ng th\u1ec3 tetrameric nh\u1eadn bi\u1ebft CD9, CD63 v\u00e0 CD81 b\u1eb1ng c\u00e1c h\u1ea1t t\u1eeb t\u00ednh. C\u00e1c EV \u0111\u00e3 \u0111\u00e1nh d\u1ea5u \u0111\u01b0\u1ee3c ph\u00e2n t\u00e1ch b\u1eb1ng nam ch\u00e2m EasySep\u2122 m\u00e0 kh\u00f4ng c\u1ea7n s\u1eed d\u1ee5ng c\u1ed9t v\u00e0 v\u1eabn \u1edf trong \u1ed1ng sau khi c\u00e1c th\u00e0nh ph\u1ea7n ch\u1ea5t l\u1ecfng sinh h\u1ecdc kh\u00f4ng mong mu\u1ed1n \u0111\u01b0\u1ee3c \u0111\u1ed5 ra.<\/p>\n<p>EV t\u1eeb ch\u1ea5t l\u1ecfng sinh h\u1ecdc, bao g\u1ed3m huy\u1ebft thanh v\u00e0 huy\u1ebft t\u01b0\u01a1ng, v\u00e0 t\u1eeb m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y, c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp trong \u00edt nh\u1ea5t 30 ph\u00fat v\u00e0 s\u1eb5n s\u00e0ng cho c\u00e1c \u1ee9ng d\u1ee5ng ti\u1ebfp theo, bao g\u1ed3m t\u00e1ch chi\u1ebft RNA, Western blot ho\u1eb7c kh\u1ed1i ph\u1ed5. S\u1ed1 li\u1ec7u d\u1eef li\u1ec7u tr\u00ean trang sau ch\u1ee9ng minh n\u0103ng su\u1ea5t, ch\u1ea5t l\u01b0\u1ee3ng v\u00e0 kh\u1ea3 n\u0103ng t\u01b0\u01a1ng th\u00edch xu\u00f4i d\u00f2ng cao c\u1ee7a EV \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng B\u1ed9 d\u1ee5ng c\u1ee5 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 \u1edf ng\u01b0\u1eddi.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Quy_trinh_thuc_hien\"><\/span><strong>Quy tr\u00ecnh th\u1ef1c hi\u1ec7n<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<div style=\"width: 643px\" class=\"wp-caption aligncenter\"><img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/roPBpuZb3gtoYL0y1wxZ35KwI2hFWhlje9a36Wyx.png\" alt=\"Quy tr\u00ecnh ph\u00e2n l\u1eadp EV kh\u1ecfi ch\u1ea5t l\u1ecfng sinh h\u1ecdc v\u1edbi B\u1ed9 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 \u1edf ng\u01b0\u1eddi\" width=\"633\" height=\"332\" \/><p class=\"wp-caption-text\"><em>H\u00ecnh 1: Quy tr\u00ecnh ph\u00e2n l\u1eadp EV kh\u1ecfi ch\u1ea5t l\u1ecfng sinh h\u1ecdc v\u1edbi B\u1ed9 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 \u1edf ng\u01b0\u1eddi<\/em><\/p><\/div>\n<div style=\"width: 446px\" class=\"wp-caption aligncenter\"><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/OHKXwKYrngsf3HgW5gsvbAYxQOFpKkTAyp0xpEKG.png\" alt=\"H\u00ecnh \u1ea3nh c\u1ee7a EV \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb huy\u1ebft t\u01b0\u01a1ng b\u1eb1ng B\u1ed9 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o c\u1ee7a EasySep\u2122\" width=\"436\" height=\"211\" \/><p class=\"wp-caption-text\"><em>H\u00ecnh 2: H\u00ecnh \u1ea3nh c\u1ee7a EV \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb huy\u1ebft t\u01b0\u01a1ng b\u1eb1ng B\u1ed9 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o c\u1ee7a EasySep\u2122<\/em><\/p><\/div>\n<p>Ph\u00e2n t\u00edch k\u00ednh hi\u1ec3n vi \u0111i\u1ec7n t\u1eed truy\u1ec1n qua (TEM) cho th\u1ea5y c\u00e1c EV c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb huy\u1ebft t\u01b0\u01a1ng c\u00f2n nguy\u00ean v\u1eb9n v\u00e0 c\u00f3 d\u1ea1ng h\u00ecnh c\u1ea7u (m\u0169i t\u00ean). C\u00e1c EV ph\u00e2n l\u1eadp \u0111\u01b0\u1ee3c g\u1eafn v\u00e0o ph\u1ee9c h\u1ee3p kh\u00e1ng th\u1ec3 tetrameric v\u00e0 c\u00e1c h\u1ea1t t\u1eeb t\u00ednh.<\/p>\n<p>Bi\u1ec3u hi\u1ec7n v\u00e0 ph\u1ee5c h\u1ed3i protein Tetraspanin c\u1ee7a EV b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng B\u1ed9 d\u1ee5ng c\u1ee5 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 Human Pan\/CD9\/CD63\/CD81.<\/p>\n<div style=\"width: 623px\" class=\"wp-caption aligncenter\"><img decoding=\"async\" class=\"\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/8FJNIJZiteXeBSMjlWMzI4dmnNimrIrBGTLximPA.png\" alt=\"T\u1ec9 l\u1ec7 thu h\u1ed3i EV bi\u1ec3u hi\u1ec7n protein Tetraspanin b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng B\u1ed9 d\u1ee5ng c\u1ee5 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 Human Pan\/CD9\/CD63\/CD81.\" width=\"613\" height=\"453\" \/><p class=\"wp-caption-text\"><em>H\u00ecnh 3: T\u1ec9 l\u1ec7 thu h\u1ed3i EV bi\u1ec3u hi\u1ec7n protein Tetraspanin b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng B\u1ed9 d\u1ee5ng c\u1ee5 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 Human Pan\/CD9\/CD63\/CD81.<\/em><\/p><\/div>\n<p>(A) EV \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb huy\u1ebft t\u01b0\u01a1ng b\u1eb1ng B\u1ed9 d\u1ee5ng c\u1ee5 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i EasySep\u2122 \u1edf ng\u01b0\u1eddi ho\u1eb7c si\u00eau ly t\u00e2m vi ph\u00e2n ng\u1eafn (2 x 70 ph\u00fat, 100.000 x g), v\u00e0 c\u00e1c ph\u1ea7n ph\u00e2n l\u1eadp \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch bi\u1ec3u hi\u1ec7n protein tetraspanin b\u1eb1ng Western blot.<\/p>\n<p>(B) T\u1ec9 l\u1ec7 thu h\u1ed3i EV t\u01b0\u01a1ng \u0111\u01b0\u01a1ng ho\u1eb7c cao h\u01a1n thu \u0111\u01b0\u1ee3c t\u1eeb d\u1ecbch n\u1ed5i m\u00f4i tr\u01b0\u1eddng MesenCult\u2122-ACF Plus \u0111\u00e3 nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o g\u1ed1c trung m\u00f4 (MSC) v\u00e0 huy\u1ebft t\u01b0\u01a1ng s\u1eed d\u1ee5ng b\u1ed9 EasySep\u2122 Human Pan Extracellular Vesicle Positive Selection Kit khi so s\u00e1nh v\u1edbi EV \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n l\u1eadp EV d\u1ef1a tr\u00ean c\u01a1 ch\u1ebf mi\u1ec5n d\u1ecbch c\u00f3 s\u1eb5n tr\u00ean th\u1ecb tr\u01b0\u1eddng kh\u00e1c.<\/p>\n<div style=\"width: 637px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/DfUYQk1QmLI7rLISpID1pQn5loWHZZ1eQtZAqRqc.png\" alt=\"10 thu\u1eadt ng\u1eef b\u1ea3n th\u1ec3 gen khoang t\u1ebf b\u00e0o h\u00e0ng \u0111\u1ea7u d\u00e0nh cho EV \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb huy\u1ebft t\u01b0\u01a1ng ho\u1eb7c d\u1ecbch n\u1ed5i m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y MSC b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng B\u1ed9 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 \u1edf ng\u01b0\u1eddi\" width=\"627\" height=\"550\" \/><p class=\"wp-caption-text\"><em>H\u00ecnh 4: 10 thu\u1eadt ng\u1eef b\u1ea3n th\u1ec3 gen khoang t\u1ebf b\u00e0o h\u00e0ng \u0111\u1ea7u d\u00e0nh cho EV \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb huy\u1ebft t\u01b0\u01a1ng ho\u1eb7c d\u1ecbch n\u1ed5i m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y MSC b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng B\u1ed9 ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh t\u00fai ngo\u1ea1i b\u00e0o EasySep\u2122 \u1edf ng\u01b0\u1eddi<\/em><\/p><\/div>\n<p>C\u00e1c protein c\u00f3 trong EV \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb (A) huy\u1ebft t\u01b0\u01a1ng v\u00e0 (B) d\u1ecbch n\u1ed5i m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y MSC s\u1eed d\u1ee5ng EasySep\u2122 Human Pan-Extracellular Vesicle Positive Selection Kit \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n b\u1eb1ng ph\u00e2n t\u00edch protein. Sau \u0111\u00f3, ch\u00fang \u0111\u01b0\u1ee3c nh\u00f3m theo thu\u1eadt ng\u1eef Gene Onology c\u1ee7a EV, x\u00e1c nh\u1eadn ch\u1ea5t l\u01b0\u1ee3ng v\u00e0 kh\u1ea3 n\u0103ng t\u01b0\u01a1ng th\u00edch c\u1ee7a EV \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp v\u1edbi ph\u00e2n t\u00edch kh\u1ed1i ph\u1ed5.<\/p>\n<div style=\"width: 639px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/N5WWa1fNMQchSMP4qv5NMkTO6gcDGddbMcWmKFnJ.png\" alt=\"C\u00e1c microRNA th\u00f4ng th\u01b0\u1eddng (miRNA) trong EV c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb huy\u1ebft t\u01b0\u01a1ng \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng b\u1ed9 EasySep\u2122 Human Pan-Extracellular Vesicle Positive Selection Kit\" width=\"629\" height=\"319\" \/><p class=\"wp-caption-text\"><em>H\u00ecnh 5: C\u00e1c microRNA th\u00f4ng th\u01b0\u1eddng (miRNA) trong EV c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb huy\u1ebft t\u01b0\u01a1ng \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng b\u1ed9 EasySep\u2122 Human Pan-Extracellular Vesicle Positive Selection Kit<\/em><\/p><\/div>\n<p>C\u00e1c microRNA (miRNA) t\u00ecm th\u1ea5y trong EV c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb huy\u1ebft t\u01b0\u01a1ng \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n b\u1eb1ng RT-qPCR ch\u1ee9ng minh kh\u1ea3 n\u0103ng t\u01b0\u01a1ng th\u00edch c\u1ee7a EasySep\u2122 Human Pan-Extracellular Vesicle Positive Selection Kitv\u1edbi qu\u00e1 tr\u00ecnh chi\u1ebft RNA v\u00e0 ph\u00e2n t\u00edch RNA \u1edf h\u1ea1 l\u01b0u. S\u1ef1 gia t\u0103ng gi\u00e1 tr\u1ecb Ct sau khi ly gi\u1ea3i EV b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng qu\u00e1 tr\u00ecnh ph\u00e1 h\u1ee7y Triton\u2122X-100 v\u00e0 RNase 0,1% ch\u1ee9ng t\u1ecf t\u00ednh to\u00e0n v\u1eb9n c\u1ee7a EV \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Thong_tin_san_pham\"><\/span><strong>Th\u00f4ng tin s\u1ea3n ph\u1ea9m<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<figure class=\"table\">\n<table>\n<tbody>\n<tr>\n<td>\n<p style=\"text-align: center;\"><strong>S\u1ea3n ph\u1ea9m<\/strong><\/p>\n<\/td>\n<td>\n<p style=\"text-align: center;\"><strong>Th\u1ec3 t\u00edch ch\u1ea5t l\u1ecfng sinh h\u1ecdc \u0111\u01b0\u1ee3c x\u1eed l\u00fd<\/strong><\/p>\n<\/td>\n<td>\n<p style=\"text-align: center;\"><strong>Catalog #<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>EasySep\u2122 Human Pan-Extracellular Vesicle Positive Selection Kit<\/td>\n<td>\n<p style=\"text-align: center;\">20 mL<\/p>\n<\/td>\n<td>\n<p style=\"text-align: center;\">17891<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>EasySep\u2122 Human Extracellular Vesicle (CD81) Positive Selection Kit<\/td>\n<td>\n<p style=\"text-align: center;\">20 mL<\/p>\n<\/td>\n<td>\n<p style=\"text-align: center;\">17892<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>EasySep\u2122 Human Extracellular Vesicle (CD9) Positive Selection Kit<\/td>\n<td>\n<p style=\"text-align: center;\">20 mL<\/p>\n<\/td>\n<td>\n<p style=\"text-align: center;\">17894<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>EasySep\u2122 Human Extracellular Vesicle (CD63) Positive Selection Kit<\/td>\n<td>\n<p style=\"text-align: center;\">20 mL<\/p>\n<\/td>\n<td>\n<p style=\"text-align: center;\">17895<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>EasySep\u2122 Extracellular Vesicle PE Positive Selection Kit<\/td>\n<td>\n<p style=\"text-align: center;\">20 mL<\/p>\n<\/td>\n<td>\n<p style=\"text-align: center;\">100-0812<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n<p>\ud83d\udce9 <strong>Th\u00f4ng tin li\u00ean h\u1ec7:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Website:<a href=\"https:\/\/biogroupvietnam.com\/vi\/lien-he\/\"> https:\/\/biogroupvietnam.com\/vi\/lien-he<\/a>\u00a0\u00a0<\/li>\n\n\n\n<li>Hotline: +84 963 621 421&nbsp;<\/li>\n\n\n\n<li>Email: info@biogroupvietnam.com<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>C\u00e1c t\u00fai ngo\u1ea1i b\u00e0o (EV), bao g\u1ed3m exosome v\u00e0 microvesicles, \u0111\u01b0\u1ee3c bao quanh b\u1edfi m\u00e0ng k\u00e9p lipid c\u00f3 c\u1ea5u tr\u00fac t\u1ef1 nhi\u00ean, \u0111\u01b0\u1ee3c gi\u1ea3i ph\u00f3ng t\u1eeb t\u1ebf b\u00e0o. EV tham gia v\u00e0o ch\u1ee9c n\u0103ng giao ti\u1ebfp gi\u1eefa c\u00e1c t\u1ebf b\u00e0o trong \u0111i\u1ec1u ki\u1ec7n c\u00e2n b\u1eb1ng n\u1ed9i m\u00f4i v\u00e0 b\u1ec7nh l\u00fd, th\u01b0\u1eddng \u0111\u01b0\u1ee3c \u0111\u1eb7c tr\u01b0ng b\u1edfi<\/p>\n<div class=\"klb-readmore post-buttons\"><a class=\"button rounded primary\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/phan-lap-va-tinh-sach-ev-voi-bo-dung-cu-chon-loc-duong-tinh-tui-ngoai-bao-easysep-cua-nguoi\/\">Read More <i class=\"klbth-icon-right-arrow\"><\/i><\/a><\/div>\n","protected":false},"author":1,"featured_media":5682,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-4216","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Ph\u00e2n l\u1eadp &amp; 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