{"id":4229,"date":"2024-02-25T10:20:29","date_gmt":"2024-02-25T10:20:29","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/2025\/08\/18\/immune-cells-isolated-with-easysep\/"},"modified":"2025-09-29T14:00:07","modified_gmt":"2025-09-29T07:00:07","slug":"phan-lap-te-bao-mien-dich-bang-easysep","status":"publish","type":"post","link":"https:\/\/biogroupvietnam.com\/vi\/blog\/phan-lap-te-bao-mien-dich-bang-easysep\/","title":{"rendered":"Ph\u00e2n L\u1eadp T\u1ebf B\u00e0o Mi\u1ec5n D\u1ecbch B\u1eb1ng EasySep\u2122"},"content":{"rendered":"<h2>C\u00f4ng c\u1ee5 ph\u00e2n l\u1eadp c\u00e1c t\u1ebf b\u00e0o s\u1ed1ng v\u00e0 c\u00f3 ch\u1ee9c n\u0103ng cho nghi\u00ean c\u1ee9u mi\u1ec5n d\u1ecbch h\u1ecdc c\u1ee7a b\u1ea1n.<\/h2>\n<h2 style=\"text-align:center\"><img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/bgAHZGp9pWQSfCCD1NM4YHkWpIH6h31tbnhgzQLr.png\" alt=\"C\u00e1c t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng nam ch\u00e2m t\u1eeb t\u00ednh&nbsp;EasySep\u2122 cho th\u1ea5y t\u1ef7 l\u1ec7&nbsp;s\u1ed1ng t\u01b0\u01a1ng \u0111\u01b0\u01a1ng v\u1edbi c\u00e1c m\u1eabu ban \u0111\u1ea7u&nbsp;\" width=\"573\" height=\"269\"><\/h2>\n<p style=\"text-align:center\"><strong>C\u00e1c t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng nam ch\u00e2m t\u1eeb t\u00ednh&nbsp;<\/strong><a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/nghien-cuu-sinh-hoc\/phan-lap-te-bao\/easysep-magnet.html\"><strong>EasySep\u2122<\/strong><\/a><strong> cho th\u1ea5y t\u1ef7 l\u1ec7&nbsp;s\u1ed1ng t\u01b0\u01a1ng \u0111\u01b0\u01a1ng v\u1edbi c\u00e1c m\u1eabu ban \u0111\u1ea7u&nbsp;<\/strong><\/p>\n<p>C\u00e1c t\u1ebf b\u00e0o mi\u1ec5n d\u1ecbch \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb c\u00e1c m\u1eabu g\u1ea1n b\u1ea1ch c\u1ea7u (leukapheresis) ho\u1eb7c m\u1eabu m\u00e1u ngo\u1ea1i vi \u0111\u00e3 \u0111\u01b0\u1ee3c x\u1eed l\u00fd b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng b\u1ed9 kit ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh ho\u1eb7c \u00e2m t\u00ednh EasySep\u2122. C\u00e1c m\u1eabu tr\u01b0\u1edbc v\u00e0 sau ph\u00e2n l\u1eadp \u0111\u01b0\u1ee3c nhu\u1ed9m b\u1eb1ng thu\u1ed1c nhu\u1ed9m 7-AAD \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 t\u1ec9 l\u1ec7 s\u1ed1ng v\u00e0 c\u00e1c marker \u0111\u1eb7c hi\u1ec7u tr\u00ean b\u1ec1 m\u1eb7t t\u1ebf b\u00e0o v\u00e0 \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ebfm t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y (flow cytometry). C\u00e1c t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng EasySep\u2122 cho th\u1ea5y t\u1ef7 l\u1ec7&nbsp;s\u1ed1ng gi\u1ea3m kh\u00f4ng \u0111\u00e1ng k\u1ec3 so v\u1edbi c\u00e1c m\u1eabu ban \u0111\u1ea7u. D\u1eef li\u1ec7u hi\u1ec3n th\u1ecb d\u01b0\u1edbi d\u1ea1ng trung b\u00ecnh \u00b1 SEM; n = 3 &#8211; 7.&nbsp;<\/p>\n<p style=\"text-align:center\"><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/lgQLYXXDuB9ZHqa746s5HdpjtfqiWajvSMW60DHO.png\" alt=\"T\u1ebf b\u00e0o T c\u1ee7a ng\u01b0\u1eddi \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng EasySep\u2122 hi\u1ec3n th\u1ecb tr\u1ea1ng th\u00e1i k\u00edch ho\u1ea1t ph\u00f9 h\u1ee3p&nbsp;\" width=\"903\" height=\"244\"><\/p>\n<p style=\"text-align:center\"><strong>T\u1ebf b\u00e0o T c\u1ee7a ng\u01b0\u1eddi \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng <\/strong><a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/nghien-cuu-sinh-hoc\/phan-lap-te-bao\/easysep-magnet.html\"><strong>EasySep\u2122<\/strong><\/a><strong> hi\u1ec3n th\u1ecb tr\u1ea1ng th\u00e1i k\u00edch ho\u1ea1t ph\u00f9 h\u1ee3p&nbsp;<\/strong><\/p>\n<p>C\u00e1c t\u1ebf b\u00e0o T \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb PBMC s\u1eed d\u1ee5ng b\u1ed9 kit ch\u1ecdn l\u1ecdc d\u01b0\u01a1ng t\u00ednh EasySep\u2122 (&#8220;Pos Sel&#8221;) ho\u1eb7c ch\u1ecdn l\u1ecdc \u00e2m t\u00ednh (&#8220;Neg Sel&#8221;) \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 bi\u1ec3u hi\u1ec7n CD25 v\u00e0 CD69 ngay sau khi ph\u00e2n l\u1eadp (Ng\u00e0y 0) v\u00e0 sau 3 ng\u00e0y nu\u00f4i c\u1ea5y c\u00f3 ho\u1eb7c kh\u00f4ng c\u00f3 b\u1ed5 sung CD3 \/CD28 k\u00edch th\u00edch. (A,C) V\u00e0o Ng\u00e0y 0, c\u00e1c t\u1ebf b\u00e0o T \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp bi\u1ec3u hi\u1ec7n m\u1ee9c CD25 v\u00e0 CD69 t\u01b0\u01a1ng t\u1ef1 so v\u1edbi c\u00e1c PBMC CD3+ kh\u00f4ng \u0111\u01b0\u1ee3c x\u1eed l\u00fd. (B, D) V\u00e0o ng\u00e0y th\u1ee9 3, c\u00e1c t\u1ebf b\u00e0o v\u1eabn kh\u00f4ng \u0111\u01b0\u1ee3c k\u00edch ho\u1ea1t khi kh\u00f4ng c\u00f3 s\u1ef1 k\u00edch th\u00edch v\u00e0 \u0111i\u1ec1u ch\u1ec9nh l\u1ea1i c\u00e1c d\u1ea5u hi\u1ec7u k\u00edch ho\u1ea1t CD25 v\u00e0 CD69 khi \u0111\u01b0\u1ee3c k\u00edch th\u00edch. D\u1eef li\u1ec7u \u0111\u01b0\u1ee3c hi\u1ec3n th\u1ecb d\u01b0\u1edbi d\u1ea1ng trung b\u00ecnh \u00b1 SEM.&nbsp;<\/p>\n<p style=\"text-align:center\"><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/zZfwefHrgpH3HP9yNVCSWherB2kPW9JEjVO9U5WE.png\" alt=\"B\u1ea1ch c\u1ea7u \u0111\u01a1n nh\u00e2n c\u1ee7a ng\u01b0\u1eddi \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng EasySep\u2122&nbsp;\" width=\"568\" height=\"531\"><\/p>\n<p style=\"text-align:center\"><strong>B\u1ea1ch c\u1ea7u \u0111\u01a1n nh\u00e2n c\u1ee7a ng\u01b0\u1eddi \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng <\/strong><a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/nghien-cuu-sinh-hoc\/phan-lap-te-bao\/easysep-magnet.html\"><strong>EasySep\u2122<\/strong><\/a><strong>&nbsp;<\/strong><\/p>\n<p>Sau khi \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng EasySep\u2122 ho\u1eb7c c\u00e1c s\u1ea3n ph\u1ea9m th\u01b0\u01a1ng m\u1ea1i kh\u00e1c, c\u00e1c t\u1ebf b\u00e0o \u0111\u01a1n nh\u00e2n \u1edf ng\u01b0\u1eddi bi\u1ec7t h\u00f3a v\u00e0 t\u0103ng tr\u01b0\u1edfng m\u1ed9t c\u00e1ch th\u00edch h\u1ee3p khi \u0111\u01b0\u1ee3c k\u00edch th\u00edch, sau \u0111\u00f3 \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y v\u00e0 bi\u1ec7t h\u00f3a th\u00e0nh c\u00e1c t\u1ebf b\u00e0o \u0111u\u00f4i gai tr\u01b0\u1edfng th\u00e0nh (DC). V\u00e0o Ng\u00e0y 0, c\u00e1c t\u1ebf b\u00e0o t\u1eeb b\u1ea1ch c\u1ea7u \u0111\u01b0\u1ee3c c\u1ea5y v\u00e0o \u0111\u0129a v\u00e0 c\u00e1c t\u1ebf b\u00e0o \u0111\u01a1n nh\u00e2n \u0111\u01b0\u1ee3c ch\u1ecdn l\u1ecdc b\u00e1m d\u00ednh trong 2 gi\u1edd. C\u00e1c t\u1ebf b\u00e0o kh\u00f4ng b\u00e1m d\u00ednh \u0111\u01b0\u1ee3c r\u1eeda s\u1ea1ch v\u00e0 c\u00e1c t\u1ebf b\u00e0o b\u00e1m d\u00ednh \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y trong 7 ng\u00e0y. V\u00e0o Ng\u00e0y th\u1ee9 5, c\u00e1c t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y v\u1edbi ch\u1ea5t b\u1ed5 sung t\u0103ng tr\u01b0\u1edfng trong 2 ng\u00e0y (DC tr\u01b0\u1edfng th\u00e0nh) ho\u1eb7c kh\u00f4ng c\u00f3 ch\u1ea5t b\u1ed5 sung t\u0103ng tr\u01b0\u1edfng (DC ch\u01b0a tr\u01b0\u1edfng th\u00e0nh). S\u1ef1 bi\u1ec3u hi\u1ec7n c\u1ee7a CD80, CD83 v\u00e0 CD86 \u1edf c\u00e1c DC ch\u01b0a tr\u01b0\u1edfng th\u00e0nh v\u00e0 tr\u01b0\u1edfng th\u00e0nh \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ebfm t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y. V\u00e0o ng\u00e0y th\u1ee9 7, c\u00e1c t\u1ebf b\u00e0o bi\u1ec3u th\u1ecb c\u00e1c d\u1ea5u hi\u1ec7u DC tr\u01b0\u1edfng th\u00e0nh CD80, CD83 v\u00e0 CD86.&nbsp;<\/p>\n<p><i>Ngu\u1ed3n | STEMCELL Technologies&nbsp;<\/i><\/p>\n","protected":false},"excerpt":{"rendered":"<p>C\u00f4ng c\u1ee5 ph\u00e2n l\u1eadp c\u00e1c t\u1ebf b\u00e0o s\u1ed1ng v\u00e0 c\u00f3 ch\u1ee9c n\u0103ng cho nghi\u00ean c\u1ee9u mi\u1ec5n d\u1ecbch h\u1ecdc c\u1ee7a b\u1ea1n. C\u00e1c t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng nam ch\u00e2m t\u1eeb t\u00ednh&nbsp;EasySep\u2122 cho th\u1ea5y t\u1ef7 l\u1ec7&nbsp;s\u1ed1ng t\u01b0\u01a1ng \u0111\u01b0\u01a1ng v\u1edbi c\u00e1c m\u1eabu ban \u0111\u1ea7u&nbsp; C\u00e1c t\u1ebf b\u00e0o mi\u1ec5n d\u1ecbch \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb c\u00e1c m\u1eabu g\u1ea1n b\u1ea1ch<\/p>\n<div class=\"klb-readmore post-buttons\"><a class=\"button rounded primary\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/phan-lap-te-bao-mien-dich-bang-easysep\/\">Read More <i class=\"klbth-icon-right-arrow\"><\/i><\/a><\/div>\n","protected":false},"author":1,"featured_media":5698,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-4229","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - 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