{"id":4249,"date":"2024-05-31T09:37:50","date_gmt":"2024-05-31T09:37:50","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/2025\/08\/18\/how-to-cryopreserve-and-thaw-cells-more-effectively\/"},"modified":"2026-03-25T23:09:15","modified_gmt":"2026-03-25T16:09:15","slug":"lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua","status":"publish","type":"post","link":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/","title":{"rendered":"L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o hi\u1ec7u qu\u1ea3?"},"content":{"rendered":"<p><span style=\"font-weight: 400;\">Qu\u00e1 tr\u00ecnh \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o \u0111\u00f3ng vai tr\u00f2 then ch\u1ed1t trong nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o, v\u00ec n\u00f3 quy\u1ebft \u0111\u1ecbnh tr\u1ef1c ti\u1ebfp t\u1ef7 l\u1ec7 s\u1ed1ng v\u00e0 kh\u1ea3 n\u0103ng s\u1ed1ng\/ ho\u1ea1t t\u00ednh sinh h\u1ecdc c\u1ee7a t\u1ebf b\u00e0o. \u0110i\u1ec1u n\u00e0y \u1ea3nh h\u01b0\u1edfng tr\u1ef1c ti\u1ebfp \u0111\u1ebfn \u0111\u1ed9 tin c\u1eady v\u00e0 t\u00ednh th\u00e0nh c\u00f4ng c\u1ee7a c\u00e1c th\u00ed nghi\u1ec7m t\u1ebf b\u00e0o h\u1ecdc ti\u1ebfp theo. \u0110\u1ec3 \u0111\u1ea3m b\u1ea3o hi\u1ec7u qu\u1ea3 t\u1ed1i \u01b0u c\u1ee7a quy tr\u00ecnh \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o, c\u1ea7n tu\u00e2n th\u1ee7 nguy\u00ean t\u1eafc c\u1ed1t l\u00f5i sau: \u0111\u00f4ng l\u1ea1nh ch\u1eadm \u2013 r\u00e3 \u0111\u00f4ng nhanh (slow freezing \u2013 rapid thawing). <\/span><\/p>\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#Dong_lanh_cham_va_ra_dong_nhanh\" >\u0110\u00f4ng l\u1ea1nh ch\u1eadm v\u00e0 r\u00e3 \u0111\u00f4ng nhanh?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#Khuyen_nghi_cho_quy_trinh_dong_lanh_te_bao\" >Khuy\u1ebfn ngh\u1ecb cho quy tr\u00ecnh \u0111\u00f4ng l\u1ea1nh t\u1ebf b\u00e0o<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#Phuc_hoi_te_bao_%E2%80%93_Khoi_phuc_suc_song_cho_te_bao\" >Ph\u1ee5c h\u1ed3i t\u1ebf b\u00e0o \u2013 Kh\u00f4i ph\u1ee5c s\u1ee9c s\u1ed1ng cho t\u1ebf b\u00e0o<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#Lay_te_bao_Cell_Retrieval\" >L\u1ea5y t\u1ebf b\u00e0o (Cell Retrieval)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#Phuong_phap_ra_dong\" >Ph\u01b0\u01a1ng ph\u00e1p r\u00e3 \u0111\u00f4ng<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#Pha_loang\" >Pha lo\u00e3ng<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#Thay_moi_truong\" >Thay m\u00f4i tr\u01b0\u1eddng<\/a><\/li><\/ul><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"Dong_lanh_cham_va_ra_dong_nhanh\"><\/span><strong>\u0110\u00f4ng l\u1ea1nh ch\u1eadm v\u00e0 r\u00e3 \u0111\u00f4ng nhanh?<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400;\">Khi t\u1ebf b\u00e0o b\u1ecb l\u00e0m l\u1ea1nh, c\u00e1c tinh th\u1ec3 b\u0103ng h\u00ecnh th\u00e0nh c\u1ea3 b\u00ean trong v\u00e0 b\u00ean ngo\u00e0i t\u1ebf b\u00e0o. N\u1ebfu t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c \u0111\u01b0a tr\u1ef1c ti\u1ebfp v\u00e0o nit\u01a1 l\u1ecfng \u1edf -196\u2103, m\u00f4i tr\u01b0\u1eddng ngo\u1ea1i b\u00e0o s\u1ebd \u0111\u00f3ng b\u0103ng tr\u01b0\u1edbc, l\u00e0m t\u0103ng d\u1ea7n n\u1ed3ng \u0111\u1ed9 dung d\u1ecbch b\u00ean ngo\u00e0i t\u1ebf b\u00e0o. \u0110i\u1ec1u n\u00e0y d\u1eabn \u0111\u1ebfn s\u1ef1 gia t\u0103ng n\u1ed3ng \u0111\u1ed9 ch\u1ea5t \u0111i\u1ec7n gi\u1ea3i (electrolytes), thay \u0111\u1ed5i \u00e1p su\u1ea5t th\u1ea9m th\u1ea5u, g\u00e2y m\u1ea5t n\u01b0\u1edbc v\u00e0 bi\u1ebfn t\u00ednh protein. Nh\u1eefng bi\u1ebfn \u0111\u1ed5i n\u00e0y c\u00f3 th\u1ec3 g\u00e2y t\u1ed5n th\u01b0\u01a1ng c\u01a1 h\u1ecdc l\u00ean c\u1ea5u tr\u00fac t\u1ebf b\u00e0o, l\u00e0m suy gi\u1ea3m t\u00ednh to\u00e0n v\u1eb9n (cell integrity).<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Trong qu\u00e1 tr\u00ecnh \u0111\u00f4ng l\u1ea1nh b\u1ea3o qu\u1ea3n t\u1ebf b\u00e0o, n\u01b0\u1edbc \u1edf m\u00f4i tr\u01b0\u1eddng ngo\u1ea1i b\u00e0o \u0111\u00f3ng b\u0103ng tr\u01b0\u1edbc. T\u1ed1c \u0111\u1ed9 l\u00e0m l\u1ea1nh kh\u00e1c nhau s\u1ebd g\u00e2y ra m\u1ee9c \u0111\u1ed9 co r\u00fat t\u1ebf b\u00e0o kh\u00e1c nhau do hi\u1ec7n t\u01b0\u1ee3ng m\u1ea5t n\u01b0\u1edbc th\u1ea9m th\u1ea5u (osmotic dehydration). Khi n\u01b0\u1edbc k\u1ebft tinh th\u00e0nh b\u0103ng, \u00e1p su\u1ea5t th\u1ea9m th\u1ea5u c\u1ee7a m\u00f4i tr\u01b0\u1eddng ngo\u1ea1i b\u00e0o ti\u1ebfp t\u1ee5c t\u0103ng. N\u1ebfu t\u1ed1c \u0111\u1ed9 l\u00e0m l\u1ea1nh qu\u00e1 ch\u1eadm, n\u01b0\u1edbc trong t\u1ebf b\u00e0o c\u00f3 nhi\u1ec1u th\u1eddi gian khu\u1ebfch t\u00e1n ra ngo\u00e0i theo c\u01a1 ch\u1ebf th\u1ea9m th\u1ea5u, d\u1eabn \u0111\u1ebfn m\u1ea5t n\u01b0\u1edbc qu\u00e1 m\u1ee9c v\u00e0 g\u00e2y ch\u1ebft t\u1ebf b\u00e0o (A). Ng\u01b0\u1ee3c l\u1ea1i, n\u1ebfu l\u00e0m l\u1ea1nh qu\u00e1 nhanh (C), n\u01b0\u1edbc b\u00ean trong t\u1ebf b\u00e0o kh\u00f4ng k\u1ecbp tho\u00e1t ra ngo\u00e0i, t\u1ea1o th\u00e0nh tinh th\u1ec3 b\u0103ng n\u1ed9i b\u00e0o (intracellular ice formation), l\u00e0m t\u1ebf b\u00e0o ch\u1ebft khi \u0111\u01b0\u1ee3c l\u00e0m \u1ea5m tr\u1edf l\u1ea1i. T\u1ed1c \u0111\u1ed9 l\u00e0m l\u1ea1nh trung gian (B) gi\u00fap c\u00e2n b\u1eb1ng gi\u1eefa nguy c\u01a1 m\u1ea5t n\u01b0\u1edbc th\u1ea9m th\u1ea5u v\u00e0 s\u1ef1 h\u00ecnh th\u00e0nh tinh th\u1ec3 b\u0103ng n\u1ed9i b\u00e0o, t\u1eeb \u0111\u00f3 th\u01b0\u1eddng \u0111\u1ea1t \u0111\u01b0\u1ee3c t\u1ef7 l\u1ec7 s\u1ed1ng s\u00f3t cao nh\u1ea5t.<\/span><\/p>\n<p><div id=\"attachment_9440\" style=\"width: 542px\" class=\"wp-caption aligncenter\"><img fetchpriority=\"high\" decoding=\"async\" aria-describedby=\"caption-attachment-9440\" class=\"size-full wp-image-9440\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2024\/05\/anh-huong-cua-toc-do-dong-lanh-den-kha-nang-song-cua-te-bao.png\" alt=\"H\u00ecnh 1: \u1ea2nh h\u01b0\u1edfng c\u1ee7a t\u1ed1c \u0111\u1ed9 \u0111\u00f4ng l\u1ea1nh \u0111\u1ebfn kh\u1ea3 n\u0103ng s\u1ed1ng c\u1ee7a t\u1ebf b\u00e0o\" width=\"532\" height=\"387\" srcset=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2024\/05\/anh-huong-cua-toc-do-dong-lanh-den-kha-nang-song-cua-te-bao.png 532w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2024\/05\/anh-huong-cua-toc-do-dong-lanh-den-kha-nang-song-cua-te-bao-300x218.png 300w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2024\/05\/anh-huong-cua-toc-do-dong-lanh-den-kha-nang-song-cua-te-bao-74x54.png 74w\" sizes=\"(max-width: 532px) 100vw, 532px\" \/><p id=\"caption-attachment-9440\" class=\"wp-caption-text\"><em>\u1ea2nh h\u01b0\u1edfng c\u1ee7a t\u1ed1c \u0111\u1ed9 \u0111\u00f4ng l\u1ea1nh \u0111\u1ebfn kh\u1ea3 n\u0103ng s\u1ed1ng c\u1ee7a t\u1ebf b\u00e0o <span style=\"font-weight: 400;\">[1]<\/span><\/em><\/p><\/div><span style=\"font-weight: 400;\">Khi b\u1ed5 sung ch\u1ea5t b\u1ea3o v\u1ec7 \u0111\u00f4ng l\u1ea1nh (cryoprotectant) nh\u01b0 DMSO (dimethyl sulfoxide) v\u00e0o m\u00f4i tr\u01b0\u1eddng \u0111\u00f4ng l\u1ea1nh, ch\u1ea5t n\u00e0y gi\u00fap h\u1ea1 \u0111i\u1ec3m \u0111\u00f3ng b\u0103ng v\u00e0 \u1ed5n \u0111\u1ecbnh \u00e1p su\u1ea5t th\u1ea9m th\u1ea5u c\u1ee7a t\u1ebf b\u00e0o. Vi\u1ec7c b\u1ed5 sung DMSO l\u00e0m gi\u1ea3m hi\u1ec7n t\u01b0\u1ee3ng co r\u00fat do m\u1ea5t n\u01b0\u1edbc trong qu\u00e1 tr\u00ecnh l\u00e0m l\u1ea1nh ch\u1eadm, h\u1ea1n ch\u1ebf s\u1ef1 h\u00ecnh th\u00e0nh tinh th\u1ec3 b\u0103ng v\u00e0 do \u0111\u00f3 gi\u1ea3m t\u1ed5n th\u01b0\u01a1ng t\u1ebf b\u00e0o [1]. <\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Khuyen_nghi_cho_quy_trinh_dong_lanh_te_bao\"><\/span><strong>Khuy\u1ebfn ngh\u1ecb cho quy tr\u00ecnh \u0111\u00f4ng l\u1ea1nh t\u1ebf b\u00e0o<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400;\">Khi h\u1ed3i ph\u1ee5c t\u1ebf b\u00e0o, c\u1ea7n t\u0103ng nhi\u1ec7t \u0111\u1ed9 nhanh v\u00e0 pha lo\u00e3ng m\u00f4i tr\u01b0\u1eddng trong v\u00f2ng 1\u20132 ph\u00fat. \u1ede th\u1eddi \u0111i\u1ec3m n\u00e0y, ch\u01b0a c\u00f3 s\u1ef1 h\u00ecnh th\u00e0nh \u0111\u00e1ng k\u1ec3 c\u1ee7a tinh th\u1ec3 b\u0103ng b\u00ean trong ho\u1eb7c b\u00ean ngo\u00e0i t\u1ebf b\u00e0o, v\u00e0 t\u1ebf b\u00e0o c\u0169ng ch\u01b0a b\u1ecb ph\u01a1i nhi\u1ec5m l\u00e2u v\u1edbi dung d\u1ecbch \u0111i\u1ec7n gi\u1ea3i n\u1ed3ng \u0111\u1ed9 cao. C\u00e1c bi\u1ec7n ph\u00e1p n\u00e0y gi\u00fap h\u1ea1n ch\u1ebf t\u1ed5n th\u01b0\u01a1ng t\u1ebf b\u00e0o v\u00e0 t\u0103ng t\u1ef7 l\u1ec7 s\u1ed1ng s\u00f3t.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Th\u1eddi \u0111i\u1ec3m t\u1ed1i \u01b0u \u0111\u1ec3 ti\u1ebfn h\u00e0nh \u0111\u00f4ng l\u1ea1nh t\u1ebf b\u00e0o l\u00e0 khi n\u00e0o? N\u1ed3ng \u0111\u1ed9 t\u1ebf b\u00e0o ph\u00f9 h\u1ee3p l\u00e0 bao nhi\u00eau? Th\u1ec3 t\u00edch m\u00f4i tr\u01b0\u1eddng b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh n\u00ean s\u1eed d\u1ee5ng bao nhi\u00eau? V\u00e0 l\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o quy tr\u00ecnh \u201c\u0111\u00f4ng ch\u1eadm\u201d \u0111\u00fang k\u1ef9 thu\u1eadt? Vui l\u00f2ng tham kh\u1ea3o b\u1ea3ng b\u00ean d\u01b0\u1edbi. <\/span><\/p>\n<figure class=\"table\">\n<table>\n<tbody>\n<tr>\n<td>Kho\u1ea3ng th\u1eddi gian t\u1ed1i \u01b0u<\/td>\n<td>\n<ul>\n<li><span style=\"font-weight: 400;\">C\u00e1c t\u1ebf b\u00e0o \u0111ang \u1edf pha t\u0103ng tr\u01b0\u1edfng l\u0169y th\u1eeba (logarithmic\/exponential growth phase), c\u00f3 t\u00ecnh tr\u1ea1ng sinh l\u00fd t\u1ed1t v\u00e0 \u0111\u00e3 cho k\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m \u1ed5n \u0111\u1ecbnh l\u00e0 \u0111\u1ed1i t\u01b0\u1ee3ng t\u1ed1i \u01b0u \u0111\u1ec3 ti\u1ebfn h\u00e0nh \u0111\u00f4ng l\u1ea1nh b\u1ea3o qu\u1ea3n.<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Khuy\u1ebfn ngh\u1ecb n\u00ean b\u1eaft \u0111\u1ea7u \u0111\u00f4ng l\u1ea1nh t\u1ebf b\u00e0o trong v\u00f2ng hai tu\u1ea7n k\u1ec3 t\u1eeb khi h\u1ed3i ph\u1ee5c (revival), \u0111\u1ed3ng th\u1eddi gi\u1eef l\u1ea1i m\u1ed9t ph\u1ea7n t\u1ebf b\u00e0o \u0111\u1ec3 ti\u1ebfp t\u1ee5c nu\u00f4i c\u1ea5y ph\u1ee5c v\u1ee5 cho c\u00e1c th\u00ed nghi\u1ec7m ti\u1ebfp theo khi c\u1ea7n thi\u1ebft.<\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>M\u00f4i tr\u01b0\u1eddng b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh<\/td>\n<td>\n<ul>\n<li><span style=\"font-weight: 400;\">M\u00f4i tr\u01b0\u1eddng b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh (cryopreservation medium) n\u00ean \u0111\u01b0\u1ee3c pha m\u1edbi ngay trong ng\u00e0y ti\u1ebfn h\u00e0nh th\u00ed nghi\u1ec7m. C\u00f4ng th\u1ee9c ti\u00eau chu\u1ea9n th\u01b0\u1eddng theo m\u1ed9t trong hai t\u1ef7 l\u1ec7: 7:2:1 gi\u1eefa m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y (culture medium), huy\u1ebft thanh (serum) v\u00e0 DMSO; ho\u1eb7c 9:1 gi\u1eefa huy\u1ebft thanh v\u00e0 DMSO.<\/span><\/li>\n<li><b>DMSO (dimethyl sulfoxide)<\/b><span style=\"font-weight: 400;\"> \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn nh\u01b0 m\u1ed9t ch\u1ea5t b\u1ea3o v\u1ec7 \u0111\u00f4ng l\u1ea1nh (cryoprotectant), v\u1edbi n\u1ed3ng \u0111\u1ed9 5\u201310% th\u01b0\u1eddng \u0111\u1ee7 \u0111\u1ec3 b\u1ea3o qu\u1ea3n ph\u1ea7n l\u1edbn c\u00e1c lo\u1ea1i t\u1ebf b\u00e0o. Do DMSO c\u00f3 t\u00ednh h\u00fat \u1ea9m (hygroscopic), c\u1ea7n b\u1ea3o qu\u1ea3n trong m\u00f4i tr\u01b0\u1eddng kh\u00f4 r\u00e1o v\u00e0 tr\u00e1nh s\u1eed d\u1ee5ng DMSO \u0111\u00e3 m\u1edf n\u1eafp qu\u00e1 m\u1ed9t n\u0103m.<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Vi\u1ec7c b\u1ed5 sung huy\u1ebft thanh v\u00e0o m\u00f4i tr\u01b0\u1eddng \u0111\u00f4ng l\u1ea1nh gi\u00fap t\u0103ng kh\u1ea3 n\u0103ng ph\u1ee5c h\u1ed3i t\u1ebf b\u00e0o sau r\u00e3 \u0111\u00f4ng. Tuy nhi\u00ean, do xu h\u01b0\u1edbng ng\u00e0y c\u00e0ng s\u1eed d\u1ee5ng m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y kh\u00f4ng ch\u1ee9a huy\u1ebft thanh (serum-free culture media), trong m\u1ed9t s\u1ed1 tr\u01b0\u1eddng h\u1ee3p c\u1ea7n \u0111\u00f4ng l\u1ea1nh t\u1ebf b\u00e0o m\u00e0 kh\u00f4ng s\u1eed d\u1ee5ng huy\u1ebft thanh. Khi \u0111\u00f3, c\u00e1c m\u00f4i tr\u01b0\u1eddng cryopreservation kh\u00f4ng huy\u1ebft thanh c\u00f3 s\u1eb5n tr\u00ean th\u1ecb tr\u01b0\u1eddng l\u00e0 m\u1ed9t gi\u1ea3i ph\u00e1p thay th\u1ebf ph\u00f9 h\u1ee3p. <\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>N\u1ed3ng \u0111\u1ed9 t\u1ebf b\u00e0o<\/td>\n<td>\n<ul>\n<li><span style=\"font-weight: 400;\">N\u1ed3ng \u0111\u1ed9 t\u1ebf b\u00e0o (Cell concentration) \u0111\u01b0\u1ee3c khuy\u1ebfn ngh\u1ecb kho\u1ea3ng 1 \u00d7 10\u2076 t\u1ebf b\u00e0o\/mL. T\u00f9y thu\u1ed9c v\u00e0o t\u1eebng lo\u1ea1i t\u1ebf b\u00e0o c\u1ee5 th\u1ec3, gi\u00e1 tr\u1ecb n\u00e0y c\u00f3 th\u1ec3 dao \u0111\u1ed9ng t\u1eeb 5 \u00d7 10\u2075 \u0111\u1ebfn 1 \u00d7 10\u2077 t\u1ebf b\u00e0o\/mL. Vi\u1ec7c t\u0103ng n\u1ed3ng \u0111\u1ed9 t\u1ebf b\u00e0o kh\u00f4ng \u0111\u1ed3ng ngh\u0129a v\u1edbi vi\u1ec7c t\u0103ng s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o s\u1ed1ng sau khi r\u00e3 \u0111\u00f4ng. Ng\u01b0\u1ee3c l\u1ea1i, n\u1ed3ng \u0111\u1ed9 qu\u00e1 cao c\u00f3 th\u1ec3 l\u00e0m suy gi\u1ea3m kh\u1ea3 n\u0103ng s\u1ed1ng (cell viability) do c\u00e1c t\u00e1c \u0111\u1ed9ng t\u00edch l\u0169y b\u1ea5t l\u1ee3i ph\u00e1t sinh t\u1eeb m\u1eadt \u0111\u1ed9 t\u1ebf b\u00e0o l\u1edbn. <\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>Th\u1ec3 t\u00edch m\u00f4i tr\u01b0\u1eddng \u0111\u00f4ng l\u1ea1nh t\u1ebf b\u00e0o<\/td>\n<td>\n<ul>\n<li><span style=\"font-weight: 400;\">L\u00fd t\u01b0\u1edfng nh\u1ea5t, m\u1ed9t \u1ed1ng cryovial ti\u00eau chu\u1ea9n 2 mL n\u00ean ch\u1ee9a 1 mL dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh. Tr\u00e1nh s\u1eed d\u1ee5ng th\u1ec3 t\u00edch qu\u00e1 l\u1edbn cho m\u1ed7i m\u1eabu, v\u00ec t\u1ed1c \u0111\u1ed9 l\u00e0m l\u1ea1nh kh\u00f4ng \u0111\u1ed3ng \u0111\u1ec1u trong to\u00e0n b\u1ed9 m\u1eabu c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng b\u1ea5t l\u1ee3i \u0111\u1ebfn kh\u1ea3 n\u0103ng s\u1ed1ng (cell viability) c\u1ee7a t\u1ebf b\u00e0o. <\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>T\u1ed1c \u0111\u1ed9 l\u00e0m l\u1ea1nh<\/td>\n<td>\n<ul>\n<li><span style=\"font-weight: 400;\">\u0110\u1ed1i v\u1edbi ph\u1ea7n l\u1edbn c\u00e1c lo\u1ea1i t\u1ebf b\u00e0o, t\u1ed1c \u0111\u1ed9 l\u00e0m l\u1ea1nh kho\u1ea3ng \u22121\u00b0C\/ph\u00fat gi\u00fap b\u1ea3o to\u00e0n kh\u1ea3 n\u0103ng s\u1ed1ng (cell viability) m\u1ed9t c\u00e1ch hi\u1ec7u qu\u1ea3. C\u00f3 th\u1ec3 s\u1eed d\u1ee5ng t\u1ee7 \u0111\u00f4ng l\u1eadp tr\u00ecnh (programmable freezers) ho\u1eb7c ph\u01b0\u01a1ng ph\u00e1p l\u00e0m l\u1ea1nh theo b\u1eadc nhi\u1ec7t (gradient cooling).<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Quy tr\u00ecnh l\u00e0m l\u1ea1nh theo b\u1eadc nhi\u1ec7t \u0111\u01b0\u1ee3c khuy\u1ebfn ngh\u1ecb nh\u01b0 sau: <\/span><span style=\"font-weight: 400;\">4\u00b0C trong 20 ph\u00fat \u2192 \u221220\u00b0C trong 30 ph\u00fat \u2192 \u221280\u00b0C qua \u0111\u00eam \u2192 sau \u0111\u00f3 chuy\u1ec3n sang l\u01b0u tr\u1eef trong nit\u01a1 l\u1ecfng.\u00a0<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Ngo\u00e0i ra, c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng c\u00e1c dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh th\u01b0\u01a1ng m\u1ea1i kh\u00f4ng y\u00eau c\u1ea7u thi\u1ebft b\u1ecb l\u1eadp tr\u00ecnh, cho ph\u00e9p l\u01b0u tr\u1eef tr\u1ef1c ti\u1ebfp \u1edf \u221280\u00b0C. <\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>\u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef<\/td>\n<td>\n<ul>\n<li><span style=\"font-weight: 400;\">\u0110\u1ed1i v\u1edbi l\u01b0u tr\u1eef ng\u1eafn h\u1ea1n, t\u1ebf b\u00e0o c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n an to\u00e0n \u1edf \u221280\u00b0C. Tuy nhi\u00ean, \u0111\u1ed1i v\u1edbi l\u01b0u tr\u1eef d\u00e0i h\u1ea1n, t\u1ebf b\u00e0o b\u1eaft bu\u1ed9c ph\u1ea3i \u0111\u01b0\u1ee3c l\u01b0u tr\u1eef trong nit\u01a1 l\u1ecfng. Vi\u1ec7c b\u1ea3o qu\u1ea3n k\u00e9o d\u00e0i \u1edf \u221280\u00b0C c\u00f3 th\u1ec3 l\u00e0m suy gi\u1ea3m kh\u1ea3 n\u0103ng s\u1ed1ng (cell viability) v\u00e0 ch\u1ee9c n\u0103ng sinh h\u1ecdc (cell functionality) c\u1ee7a t\u1ebf b\u00e0o. <\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>D\u00e1n nh\u00e3n v\u00e0 l\u01b0u h\u1ed3 s\u01a1<\/td>\n<td>\n<ul>\n<li><span style=\"font-weight: 400;\">\u0110\u1ea3m b\u1ea3o \u1ed1ng cryovial \u0111\u01b0\u1ee3c ghi nh\u00e3n r\u00f5 r\u00e0ng v\u1edbi c\u00e1c th\u00f4ng tin c\u1ea7n thi\u1ebft nh\u01b0: t\u00ean d\u00f2ng t\u1ebf b\u00e0o (cell name), s\u1ed1 th\u1ebf h\u1ec7\/chuy\u1ec3n \u0111\u1eddi (passage number ho\u1eb7c generation), ng\u01b0\u1eddi th\u1ef1c hi\u1ec7n \u0111\u00f4ng l\u1ea1nh v\u00e0 ng\u00e0y ti\u1ebfn h\u00e0nh \u0111\u00f4ng l\u1ea1nh.<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Ngo\u00e0i ra, c\u1ea7n ghi ch\u00e9p \u0111\u1ea7y \u0111\u1ee7 th\u00f4ng tin t\u1ebf b\u00e0o c\u00f9ng v\u1edbi v\u1ecb tr\u00ed l\u01b0u tr\u1eef ch\u00ednh x\u00e1c trong s\u1ed5 theo d\u00f5i ho\u1eb7c h\u1ec7 th\u1ed1ng qu\u1ea3n l\u00fd m\u1eabu. Vi\u1ec7c qu\u1ea3n l\u00fd h\u1ed3 s\u01a1 ch\u1eb7t ch\u1ebd nh\u01b0 v\u1eady gi\u00fap \u0111\u1ea3m b\u1ea3o c\u00f3 th\u1ec3 truy xu\u1ea5t \u0111\u00fang m\u1eabu t\u1ebf b\u00e0o m\u1ed9t c\u00e1ch thu\u1eadn ti\u1ec7n khi c\u1ea7n r\u00e3 \u0111\u00f4ng. <\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<h2><span class=\"ez-toc-section\" id=\"Phuc_hoi_te_bao_%E2%80%93_Khoi_phuc_suc_song_cho_te_bao\"><\/span><strong>Ph\u1ee5c h\u1ed3i t\u1ebf b\u00e0o \u2013 Kh\u00f4i ph\u1ee5c s\u1ee9c s\u1ed1ng cho t\u1ebf b\u00e0o<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<h3><span class=\"ez-toc-section\" id=\"Lay_te_bao_Cell_Retrieval\"><\/span>L\u1ea5y t\u1ebf b\u00e0o (Cell Retrieval)<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400;\">C\u1ea7n gi\u1ea3m thi\u1ec3u t\u1ed1i \u0111a th\u1eddi gian m\u1eabu b\u1ecb \u1ea5m l\u00ean th\u1ee5 \u0111\u1ed9ng t\u1eeb l\u00fac l\u1ea5y ra kh\u1ecfi nit\u01a1 l\u1ecfng ho\u1eb7c t\u1ee7 \u221280\u2103 cho \u0111\u1ebfn khi \u0111\u01b0a v\u00e0o thi\u1ebft b\u1ecb r\u00e3 \u0111\u00f4ng, nh\u1eb1m tr\u00e1nh \u1ea3nh h\u01b0\u1edfng ti\u00eau c\u1ef1c \u0111\u1ebfn kh\u1ea3 n\u0103ng s\u1ed1ng (cell vitality).\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">N\u1ebfu v\u1ecb tr\u00ed l\u01b0u tr\u1eef v\u00e0 v\u1ecb tr\u00ed r\u00e3 \u0111\u00f4ng c\u00e1ch xa nhau, c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng th\u00f9ng c\u00e1ch nhi\u1ec7t ch\u1ee9a \u0111\u00e1 kh\u00f4 (\u221280\u2103) ho\u1eb7c nit\u01a1 l\u1ecfng (\u2212196\u2103) \u0111\u1ec3 b\u1ea3o qu\u1ea3n t\u1ea1m th\u1eddi v\u00e0 v\u1eadn chuy\u1ec3n m\u1eabu t\u1ebf b\u00e0o.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">(L\u01b0u \u00fd: Lu\u00f4n \u0111eo k\u00ednh b\u1ea3o h\u1ed9 v\u00e0 g\u0103ng tay khi l\u1ea5y t\u1ebf b\u00e0o kh\u1ecfi nit\u01a1 l\u1ecfng \u0111\u1ec3 tr\u00e1nh b\u1ecfng l\u1ea1nh v\u00e0 nguy c\u01a1 n\u1ed5 \u1ed1ng cryovial do \u00e1p su\u1ea5t t\u0103ng!) <\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Phuong_phap_ra_dong\"><\/span>Ph\u01b0\u01a1ng ph\u00e1p r\u00e3 \u0111\u00f4ng<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400;\">a) Trong qu\u00e1 tr\u00ecnh r\u00e3 \u0111\u00f4ng, t\u1ebf b\u00e0o c\u1ea7n \u0111\u01b0\u1ee3c l\u00e0m \u1ea5m nhanh v\u1edbi t\u1ed1c \u0111\u1ed9 t\u0103ng nhi\u1ec7t kho\u1ea3ng 50\u2013100\u2103\/ph\u00fat \u0111\u1ec3 t\u1ed1i \u0111a h\u00f3a kh\u1ea3 n\u0103ng s\u1ed1ng. Ph\u01b0\u01a1ng ph\u00e1p t\u1ed1i \u01b0u l\u00e0 s\u1eed d\u1ee5ng b\u1ec3 n\u01b0\u1edbc 37\u2103 [3].\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Sau khi l\u1ea5y \u1ed1ng t\u1ebf b\u00e0o \u0111\u00f4ng l\u1ea1nh kh\u1ecfi nit\u01a1 l\u1ecfng ho\u1eb7c t\u1ee7 \u221280\u2103, n\u00ean n\u1edbi l\u1ecfng nh\u1eb9 n\u1eafp \u1ed1ng \u0111\u1ec3 tr\u00e1nh n\u1ed5 \u1ed1ng cryovial trong qu\u00e1 tr\u00ecnh r\u00e3 \u0111\u00f4ng. \u0110\u1eb7t \u1ed1ng v\u00e0o b\u1ec3 n\u01b0\u1edbc 37\u2103 v\u00e0 l\u1eafc m\u1ea1nh trong 1\u20132 ph\u00fat cho \u0111\u1ebfn khi tan ho\u00e0n to\u00e0n.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Khuy\u1ebfn ngh\u1ecb d\u1eebng r\u00e3 \u0111\u00f4ng khi ph\u1ea7n b\u0103ng nh\u00ecn th\u1ea5y \u0111\u00e3 tan h\u1ebft nh\u01b0ng m\u1eabu v\u1eabn c\u00f2n \u1edf tr\u1ea1ng th\u00e1i r\u1ea5t l\u1ea1nh (~0\u2103). Th\u1ef1c h\u00e0nh ph\u1ed5 bi\u1ebfn l\u00e0 l\u1ea5y \u1ed1ng ra kh\u1ecfi b\u1ec3 n\u01b0\u1edbc khi ch\u1ec9 c\u00f2n m\u1ed9t m\u1ea3nh b\u0103ng nh\u1ecf. Vi\u1ec7c l\u00e0m \u1ea5m \u0111\u1ebfn nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng ho\u1eb7c cao h\u01a1n s\u1ebd l\u00e0m t\u0103ng \u0111\u1ed9c t\u00ednh t\u1ebf b\u00e0o (cytotoxicity) c\u1ee7a ch\u1ea5t b\u1ea3o v\u1ec7 \u0111\u00f4ng l\u1ea1nh (cryoprotectant) v\u00e0 l\u00e0m suy gi\u1ea3m nghi\u00eam tr\u1ecdng kh\u1ea3 n\u0103ng s\u1ed1ng c\u1ee7a t\u1ebf b\u00e0o [1].\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">b) Kh\u00f4ng khuy\u1ebfn kh\u00edch r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng v\u00ec c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn ch\u1ebft t\u1ebf b\u00e0o.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">c) \u0110\u1ec3 gi\u1ea3m nguy c\u01a1 nhi\u1ec5m t\u1ea1p (contamination), c\u1ea7n \u0111\u1ea3m b\u1ea3o mi\u1ec7ng \u1ed1ng cryovial lu\u00f4n \u1edf tr\u00ean m\u1ef1c n\u01b0\u1edbc trong qu\u00e1 tr\u00ecnh r\u00e3 \u0111\u00f4ng b\u1eb1ng b\u1ec3 n\u01b0\u1edbc, tr\u00e1nh n\u01b0\u1edbc x\u00e2m nh\u1eadp v\u00e0o b\u00ean trong \u1ed1ng. N\u01b0\u1edbc v\u00f4 tr\u00f9ng trong b\u1ec3 c\u0169ng c\u1ea7n \u0111\u01b0\u1ee3c thay \u0111\u1ecbnh k\u1ef3. <\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Pha_loang\"><\/span>Pha lo\u00e3ng<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400;\">C\u1ea7n pha lo\u00e3ng t\u1ebf b\u00e0o c\u00e0ng s\u1edbm c\u00e0ng t\u1ed1t sau khi r\u00e3 \u0111\u00f4ng \u0111\u1ec3 gi\u1ea3m \u0111\u1ed9c t\u00ednh c\u1ee7a DMSO. Th\u00eam m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y v\u1edbi t\u1ef7 l\u1ec7 m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y : m\u00f4i tr\u01b0\u1eddng b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh \u2265 10:1 \u0111\u1ec3 pha lo\u00e3ng, sau \u0111\u00f3 ly t\u00e2m (centrifuge) v\u00e0 thay m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y m\u1edbi \u0111\u1ec3 t\u00e1i huy\u1ec1n ph\u00f9 (resuspend) t\u1ebf b\u00e0o.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Quy tr\u00ecnh n\u00e0y gi\u00fap gi\u1ea3m stress t\u1ebf b\u00e0o do thay \u0111\u1ed5i \u00e1p su\u1ea5t th\u1ea9m th\u1ea5u (osmotic stress). <\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Thay_moi_truong\"><\/span>Thay m\u00f4i tr\u01b0\u1eddng<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400;\">Ti\u1ebfn h\u00e0nh thay m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y v\u00e0 ti\u1ebfp t\u1ee5c nu\u00f4i t\u1ebf b\u00e0o sau khi t\u1ebf b\u00e0o \u0111\u00e3 b\u00e1m d\u00ednh (cell attachment) ho\u1eb7c sau 24 gi\u1edd, t\u00f9y thu\u1ed9c v\u00e0o t\u00ecnh tr\u1ea1ng v\u00e0 \u0111\u1eb7c \u0111i\u1ec3m sinh tr\u01b0\u1edfng c\u1ee7a d\u00f2ng t\u1ebf b\u00e0o. <\/span><\/p>\n<p class=\"pulse-title text-display-md text-color-text lg:text-display-lg font-bold w-11\/12\"><em>Xem th\u00eam:<\/em> <em><a href=\"https:\/\/biogroupvietnam.com\/vi\/danh-muc-san-pham\/bao-quan-lanh-te-bao\/\">Gi\u1ea3i ph\u00e1p \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o t\u1eeb Biogroup<\/a><\/em><\/p>\n<p><strong>NGU\u1ed2N THAM KH\u1ea2O<\/strong><\/p>\n<p><em><a href=\"https:\/\/www.linkedin.com\/pulse\/how-cryopreserve-thaw-cells-more-effectively-medchemexpress-llc-zsp0f\/\">How to Cryopreserve and Thaw Cells More Effectively<\/a><\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Qu\u00e1 tr\u00ecnh \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o \u0111\u00f3ng vai tr\u00f2 then ch\u1ed1t trong nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o, v\u00ec n\u00f3 quy\u1ebft \u0111\u1ecbnh tr\u1ef1c ti\u1ebfp t\u1ef7 l\u1ec7 s\u1ed1ng v\u00e0 kh\u1ea3 n\u0103ng s\u1ed1ng\/ ho\u1ea1t t\u00ednh sinh h\u1ecdc c\u1ee7a t\u1ebf b\u00e0o. \u0110i\u1ec1u n\u00e0y \u1ea3nh h\u01b0\u1edfng tr\u1ef1c ti\u1ebfp \u0111\u1ebfn \u0111\u1ed9 tin c\u1eady v\u00e0 t\u00ednh th\u00e0nh c\u00f4ng c\u1ee7a c\u00e1c<\/p>\n<div class=\"klb-readmore post-buttons\"><a class=\"button rounded primary\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/\">Read More <i class=\"klbth-icon-right-arrow\"><\/i><\/a><\/div>\n","protected":false},"author":1,"featured_media":5723,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-4249","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o hi\u1ec7u qu\u1ea3?<\/title>\n<meta name=\"description\" content=\"Qu\u00e1 tr\u00ecnh \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o s\u1ebd quy\u1ebft \u0111\u1ecbnh tr\u1ef1c ti\u1ebfp \u0111\u1ebfn kh\u1ea3 n\u0103ng s\u1ed1ng c\u1ee7a t\u1ebf b\u00e0o. Nguy\u00ean t\u1eafc thi\u1ebft y\u1ebfu \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o hi\u1ec7u qu\u1ea3 t\u1ed1i \u01b0u: \u0110\u00f4ng l\u1ea1nh ch\u1eadm v\u00e0 r\u00e3 \u0111\u00f4ng nhanh.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/\" \/>\n<meta property=\"og:locale\" content=\"vi_VN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o hi\u1ec7u qu\u1ea3?\" \/>\n<meta property=\"og:description\" content=\"Qu\u00e1 tr\u00ecnh \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o s\u1ebd quy\u1ebft \u0111\u1ecbnh tr\u1ef1c ti\u1ebfp \u0111\u1ebfn kh\u1ea3 n\u0103ng s\u1ed1ng c\u1ee7a t\u1ebf b\u00e0o. Nguy\u00ean t\u1eafc thi\u1ebft y\u1ebfu \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o hi\u1ec7u qu\u1ea3 t\u1ed1i \u01b0u: \u0110\u00f4ng l\u1ea1nh ch\u1eadm v\u00e0 r\u00e3 \u0111\u00f4ng nhanh.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/\" \/>\n<meta property=\"og:site_name\" content=\"Biogroup Vietnam\" \/>\n<meta property=\"article:published_time\" content=\"2024-05-31T09:37:50+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-03-25T16:09:15+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/p6uVsjc42Hwu6fUw20BjcwdWQWH5xXmWg53uIdVp.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1080\" \/>\n\t<meta property=\"og:image:height\" content=\"720\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"\u0110\u01b0\u1ee3c vi\u1ebft b\u1edfi\" \/>\n\t<meta name=\"twitter:data1\" content=\"admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u01af\u1edbc t\u00ednh th\u1eddi gian \u0111\u1ecdc\" \/>\n\t<meta name=\"twitter:data2\" content=\"11 ph\u00fat\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/\"},\"author\":{\"name\":\"admin\",\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/#\/schema\/person\/78a1c640012e6e7bc2bdf0d0fbffea95\"},\"headline\":\"L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o hi\u1ec7u qu\u1ea3?\",\"datePublished\":\"2024-05-31T09:37:50+00:00\",\"dateModified\":\"2026-03-25T16:09:15+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/\"},\"wordCount\":2765,\"image\":{\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/p6uVsjc42Hwu6fUw20BjcwdWQWH5xXmWg53uIdVp.jpg\",\"articleSection\":[\"Blog\"],\"inLanguage\":\"vi\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/\",\"url\":\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/\",\"name\":\"L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o hi\u1ec7u qu\u1ea3?\",\"isPartOf\":{\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/p6uVsjc42Hwu6fUw20BjcwdWQWH5xXmWg53uIdVp.jpg\",\"datePublished\":\"2024-05-31T09:37:50+00:00\",\"dateModified\":\"2026-03-25T16:09:15+00:00\",\"author\":{\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/#\/schema\/person\/78a1c640012e6e7bc2bdf0d0fbffea95\"},\"description\":\"Qu\u00e1 tr\u00ecnh \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o s\u1ebd quy\u1ebft \u0111\u1ecbnh tr\u1ef1c ti\u1ebfp \u0111\u1ebfn kh\u1ea3 n\u0103ng s\u1ed1ng c\u1ee7a t\u1ebf b\u00e0o. Nguy\u00ean t\u1eafc thi\u1ebft y\u1ebfu \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o hi\u1ec7u qu\u1ea3 t\u1ed1i \u01b0u: \u0110\u00f4ng l\u1ea1nh ch\u1eadm v\u00e0 r\u00e3 \u0111\u00f4ng nhanh.\",\"breadcrumb\":{\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#breadcrumb\"},\"inLanguage\":\"vi\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"vi\",\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#primaryimage\",\"url\":\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/p6uVsjc42Hwu6fUw20BjcwdWQWH5xXmWg53uIdVp.jpg\",\"contentUrl\":\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/p6uVsjc42Hwu6fUw20BjcwdWQWH5xXmWg53uIdVp.jpg\",\"width\":1080,\"height\":720,\"caption\":\"How to Cryopreserve and Thaw Cells More Effectively?\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Trang ch\u1ee7\",\"item\":\"https:\/\/biogroupvietnam.com\/vi\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Blog\",\"item\":\"https:\/\/biogroupvietnam.com\/vi\/su-kien-va-tin-tuc\/blog\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o hi\u1ec7u qu\u1ea3?\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/#website\",\"url\":\"https:\/\/biogroupvietnam.com\/vi\/\",\"name\":\"Biogroup Vietnam\",\"description\":\"Biogroup Vietnam cung c\u1ea5p d\u1ecbch v\u1ee5 k\u1ef9 thu\u1eadt c\u00f9ng thi\u1ebft b\u1ecb, v\u1eadt t\u01b0, h\u00f3a ch\u1ea5t v\u00e0 gi\u1ea3i ph\u00e1p sinh h\u1ecdc cho ph\u00f2ng th\u00ed nghi\u1ec7m c\u00f4ng ngh\u1ec7 sinh h\u1ecdc, y sinh v\u00e0 y h\u1ecdc t\u00e1i t\u1ea1o.\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/biogroupvietnam.com\/vi\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"vi\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/#\/schema\/person\/78a1c640012e6e7bc2bdf0d0fbffea95\",\"name\":\"admin\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"vi\",\"@id\":\"https:\/\/biogroupvietnam.com\/vi\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/biogroupvietnam.com\/wp-content\/litespeed\/avatar\/eb8a826cba57879e3cdbbc4f5ece34eb.jpg?ver=1776157819\",\"contentUrl\":\"https:\/\/biogroupvietnam.com\/wp-content\/litespeed\/avatar\/eb8a826cba57879e3cdbbc4f5ece34eb.jpg?ver=1776157819\",\"caption\":\"admin\"},\"sameAs\":[\"https:\/\/freshfromnature.vn\/\"],\"url\":\"https:\/\/biogroupvietnam.com\/vi\/author\/admin\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o hi\u1ec7u qu\u1ea3?","description":"Qu\u00e1 tr\u00ecnh \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o s\u1ebd quy\u1ebft \u0111\u1ecbnh tr\u1ef1c ti\u1ebfp \u0111\u1ebfn kh\u1ea3 n\u0103ng s\u1ed1ng c\u1ee7a t\u1ebf b\u00e0o. Nguy\u00ean t\u1eafc thi\u1ebft y\u1ebfu \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o hi\u1ec7u qu\u1ea3 t\u1ed1i \u01b0u: \u0110\u00f4ng l\u1ea1nh ch\u1eadm v\u00e0 r\u00e3 \u0111\u00f4ng nhanh.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/","og_locale":"vi_VN","og_type":"article","og_title":"L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o hi\u1ec7u qu\u1ea3?","og_description":"Qu\u00e1 tr\u00ecnh \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o s\u1ebd quy\u1ebft \u0111\u1ecbnh tr\u1ef1c ti\u1ebfp \u0111\u1ebfn kh\u1ea3 n\u0103ng s\u1ed1ng c\u1ee7a t\u1ebf b\u00e0o. Nguy\u00ean t\u1eafc thi\u1ebft y\u1ebfu \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o hi\u1ec7u qu\u1ea3 t\u1ed1i \u01b0u: \u0110\u00f4ng l\u1ea1nh ch\u1eadm v\u00e0 r\u00e3 \u0111\u00f4ng nhanh.","og_url":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/","og_site_name":"Biogroup Vietnam","article_published_time":"2024-05-31T09:37:50+00:00","article_modified_time":"2026-03-25T16:09:15+00:00","og_image":[{"width":1080,"height":720,"url":"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/p6uVsjc42Hwu6fUw20BjcwdWQWH5xXmWg53uIdVp.jpg","type":"image\/jpeg"}],"author":"admin","twitter_card":"summary_large_image","twitter_misc":{"\u0110\u01b0\u1ee3c vi\u1ebft b\u1edfi":"admin","\u01af\u1edbc t\u00ednh th\u1eddi gian \u0111\u1ecdc":"11 ph\u00fat"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#article","isPartOf":{"@id":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/"},"author":{"name":"admin","@id":"https:\/\/biogroupvietnam.com\/vi\/#\/schema\/person\/78a1c640012e6e7bc2bdf0d0fbffea95"},"headline":"L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o hi\u1ec7u qu\u1ea3?","datePublished":"2024-05-31T09:37:50+00:00","dateModified":"2026-03-25T16:09:15+00:00","mainEntityOfPage":{"@id":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/"},"wordCount":2765,"image":{"@id":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#primaryimage"},"thumbnailUrl":"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/p6uVsjc42Hwu6fUw20BjcwdWQWH5xXmWg53uIdVp.jpg","articleSection":["Blog"],"inLanguage":"vi"},{"@type":"WebPage","@id":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/","url":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/","name":"L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o hi\u1ec7u qu\u1ea3?","isPartOf":{"@id":"https:\/\/biogroupvietnam.com\/vi\/#website"},"primaryImageOfPage":{"@id":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#primaryimage"},"image":{"@id":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#primaryimage"},"thumbnailUrl":"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/p6uVsjc42Hwu6fUw20BjcwdWQWH5xXmWg53uIdVp.jpg","datePublished":"2024-05-31T09:37:50+00:00","dateModified":"2026-03-25T16:09:15+00:00","author":{"@id":"https:\/\/biogroupvietnam.com\/vi\/#\/schema\/person\/78a1c640012e6e7bc2bdf0d0fbffea95"},"description":"Qu\u00e1 tr\u00ecnh \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o s\u1ebd quy\u1ebft \u0111\u1ecbnh tr\u1ef1c ti\u1ebfp \u0111\u1ebfn kh\u1ea3 n\u0103ng s\u1ed1ng c\u1ee7a t\u1ebf b\u00e0o. Nguy\u00ean t\u1eafc thi\u1ebft y\u1ebfu \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o hi\u1ec7u qu\u1ea3 t\u1ed1i \u01b0u: \u0110\u00f4ng l\u1ea1nh ch\u1eadm v\u00e0 r\u00e3 \u0111\u00f4ng nhanh.","breadcrumb":{"@id":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#breadcrumb"},"inLanguage":"vi","potentialAction":[{"@type":"ReadAction","target":["https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/"]}]},{"@type":"ImageObject","inLanguage":"vi","@id":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#primaryimage","url":"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/p6uVsjc42Hwu6fUw20BjcwdWQWH5xXmWg53uIdVp.jpg","contentUrl":"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/p6uVsjc42Hwu6fUw20BjcwdWQWH5xXmWg53uIdVp.jpg","width":1080,"height":720,"caption":"How to Cryopreserve and Thaw Cells More Effectively?"},{"@type":"BreadcrumbList","@id":"https:\/\/biogroupvietnam.com\/vi\/blog\/lam-the-nao-de-dong-lanh-va-ra-dong-te-bao-hieu-qua\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Trang ch\u1ee7","item":"https:\/\/biogroupvietnam.com\/vi\/"},{"@type":"ListItem","position":2,"name":"Blog","item":"https:\/\/biogroupvietnam.com\/vi\/su-kien-va-tin-tuc\/blog\/"},{"@type":"ListItem","position":3,"name":"L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o hi\u1ec7u qu\u1ea3?"}]},{"@type":"WebSite","@id":"https:\/\/biogroupvietnam.com\/vi\/#website","url":"https:\/\/biogroupvietnam.com\/vi\/","name":"Biogroup Vietnam","description":"Biogroup Vietnam cung c\u1ea5p d\u1ecbch v\u1ee5 k\u1ef9 thu\u1eadt c\u00f9ng thi\u1ebft b\u1ecb, v\u1eadt t\u01b0, h\u00f3a ch\u1ea5t v\u00e0 gi\u1ea3i ph\u00e1p sinh h\u1ecdc cho ph\u00f2ng th\u00ed nghi\u1ec7m c\u00f4ng ngh\u1ec7 sinh h\u1ecdc, y sinh v\u00e0 y h\u1ecdc t\u00e1i t\u1ea1o.","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/biogroupvietnam.com\/vi\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"vi"},{"@type":"Person","@id":"https:\/\/biogroupvietnam.com\/vi\/#\/schema\/person\/78a1c640012e6e7bc2bdf0d0fbffea95","name":"admin","image":{"@type":"ImageObject","inLanguage":"vi","@id":"https:\/\/biogroupvietnam.com\/vi\/#\/schema\/person\/image\/","url":"https:\/\/biogroupvietnam.com\/wp-content\/litespeed\/avatar\/eb8a826cba57879e3cdbbc4f5ece34eb.jpg?ver=1776157819","contentUrl":"https:\/\/biogroupvietnam.com\/wp-content\/litespeed\/avatar\/eb8a826cba57879e3cdbbc4f5ece34eb.jpg?ver=1776157819","caption":"admin"},"sameAs":["https:\/\/freshfromnature.vn\/"],"url":"https:\/\/biogroupvietnam.com\/vi\/author\/admin\/"}]}},"_links":{"self":[{"href":"https:\/\/biogroupvietnam.com\/vi\/wp-json\/wp\/v2\/posts\/4249","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biogroupvietnam.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biogroupvietnam.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biogroupvietnam.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biogroupvietnam.com\/vi\/wp-json\/wp\/v2\/comments?post=4249"}],"version-history":[{"count":13,"href":"https:\/\/biogroupvietnam.com\/vi\/wp-json\/wp\/v2\/posts\/4249\/revisions"}],"predecessor-version":[{"id":10073,"href":"https:\/\/biogroupvietnam.com\/vi\/wp-json\/wp\/v2\/posts\/4249\/revisions\/10073"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biogroupvietnam.com\/vi\/wp-json\/wp\/v2\/media\/5723"}],"wp:attachment":[{"href":"https:\/\/biogroupvietnam.com\/vi\/wp-json\/wp\/v2\/media?parent=4249"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biogroupvietnam.com\/vi\/wp-json\/wp\/v2\/categories?post=4249"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biogroupvietnam.com\/vi\/wp-json\/wp\/v2\/tags?post=4249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}