M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd FPLC d\u1ef1a tr\u00ean c\u00e1c h\u1ea1t agarose li\u00ean k\u1ebft ch\u00e9o, v\u1edbi \u0111\u01b0\u1eddng k\u00ednh trung b\u00ecnh 100 \u03bcm. H\u1ea1t s\u1eafc k\u00fd \u0111\u01b0\u1ee3c g\u1eafn c\u00e1c ligand ch\u1ee9c n\u0103ng \u00e1i l\u1ef1c Protein A ho\u1eb7c Protein G \u0111\u1ec3 li\u00ean k\u1ebft (b\u1eaft) v\u1edbi c\u00e1c ph\u00e2n \u0111o\u1ea1n kh\u00e1ng th\u1ec3 ho\u1eb7c kh\u00e1ng nguy\u00ean.<\/p>\n
M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd \u00e1i l\u1ef1c Sepapure\u00ae d\u00f9ng tinh s\u1ea1ch c\u00e1c \u0111o\u1ea1n kh\u00e1ng th\u1ec3 ho\u1eb7c kh\u00e1ng nguy\u00ean \u0111\u01b0\u1ee3c s\u1ea3n xu\u1ea5t \u0111\u1ec3 d\u00f9ng v\u1edbi nhi\u1ec1u lo\u1ea1i dung d\u1ecbch \u0111\u1ec7m trong tinh ch\u1ebf kh\u00e1ng th\u1ec3. M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd \u00e1i l\u1ef1c Sepapure\u00ae c\u00f3 h\u1ea1n s\u1eed d\u1ee5ng l\u00e2u d\u00e0i khi \u0111\u01b0\u1ee3c thao t\u00e1c v\u00e0 b\u1ea3o qu\u1ea3n \u0111\u00fang c\u00e1ch v\u00e0 t\u01b0\u01a1ng th\u00edch v\u1edbi h\u1ea7u h\u1ebft ph\u01b0\u01a1ng ph\u00e1p ti\u1ec7t tr\u00f9ng CIP. T\u1ea5t c\u1ea3 m\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd \u00e1i l\u1ef1c Sepapure\u00ae \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u1edf dung d\u1ecbch ethanol 20%.<\/p>\n
H\u1ea1t s\u1eafc k\u00fd \u00e1i l\u1ef1c Sepapure\u00ae c\u00f3 kh\u1ea3 n\u0103ng b\u00e1m (b\u1eaft protein) t\u1eeb 1-30 mg\/l (\u0111\u1ed1i v\u1edbi c\u1ed9t s\u1eafc k\u00fd Protein A) ho\u1eb7c 1-15 mg\/ml (\u0111\u1ed1i v\u1edbi c\u1ed9t s\u1eafc k\u00fd Protein G). \u00c1p su\u1ea5t v\u1eadn h\u00e0nh t\u1ed1i \u0111a cho c\u1ed9t s\u1eafc k\u00fd Sepapure\u00ae n\u00ean s\u1eed d\u1ee5ng \u1edf \u00e1p su\u1ea5t 3 bar, t\u1ed1c \u0111\u1ed9 d\u00f2ng ch\u1ea3y khuy\u1ebfn ngh\u1ecb l\u00e0 1 CV\/ml.<\/p>\n
H\u1ea1t s\u1eafc k\u00fd \u00e1i l\u1ef1c Sepapure\u00ae th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u1edf b\u01b0\u1edbc \u201ccapture\u201d trong quy tr\u00ecnh tinh s\u1ea1ch protein.<\/p>\n
M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd FPLC d\u1ef1a tr\u00ean c\u00e1c h\u1ea1t agarose li\u00ean k\u1ebft ch\u00e9o, v\u1edbi \u0111\u01b0\u1eddng k\u00ednh trung b\u00ecnh 100 \u03bcm. H\u1ea1t s\u1eafc k\u00fd \u0111\u01b0\u1ee3c g\u1eafn c\u00e1c ligand ch\u1ee9c n\u0103ng \u00e1i l\u1ef1c Protein A ho\u1eb7c Protein G \u0111\u1ec3 li\u00ean k\u1ebft (b\u1eaft) v\u1edbi c\u00e1c ph\u00e2n \u0111o\u1ea1n kh\u00e1ng th\u1ec3 ho\u1eb7c kh\u00e1ng nguy\u00ean. \u0110\u1eb7c t\u00ednh M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd<\/p>\n