{"id":3735,"date":"2025-08-18T19:52:47","date_gmt":"2025-08-18T12:52:47","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/product\/import-placeholder-for-85\/"},"modified":"2026-04-06T13:17:22","modified_gmt":"2026-04-06T06:17:22","slug":"dung-dich-ammonium-chloride","status":"publish","type":"product","link":"https:\/\/biogroupvietnam.com\/vi\/san-pham\/dung-dich-ammonium-chloride\/","title":{"rendered":"Dung D\u1ecbch Ammonium Chloride Ly Gi\u1ea3i H\u1ed3ng C\u1ea7u"},"content":{"rendered":"<p style=\"text-align: justify;\">Dung d\u1ecbch Ammonium Chloride \u0111\u01b0\u1ee3c khuy\u1ebfn ngh\u1ecb d\u00f9ng \u0111\u1ec3 ly gi\u1ea3i t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u (RBCs) trong c\u00e1c ch\u1ebf ph\u1ea9m c\u1ee7a m\u00e1u ngo\u1ea1i vi ng\u01b0\u1eddi v\u00e0 chu\u1ed9t, l\u00e1 l\u00e1ch ho\u1eb7c t\u1ebf b\u00e0o t\u1ee7y x\u01b0\u01a1ng c\u1ee7a chu\u1ed9t. Ammonium Chloride \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng nh\u01b0 dung d\u1ecbch \u0111\u1ec7m, t\u1ed1i \u01b0u h\u00f3a \u0111\u1ec3 ly gi\u1ea3i h\u1ed3ng c\u1ea7u m\u1ed9t c\u00e1ch nh\u1eb9 nh\u00e0ng trong khi t\u00e1c d\u1ee5ng t\u1ed1i thi\u1ec3u l\u00ean b\u1ea1ch c\u1ea7u. N\u00f3 kh\u00f4ng ch\u1ee9a t\u00e1c nh\u00e2n c\u1ed1 \u0111\u1ecbnh, do \u0111\u00f3 b\u1ea1ch c\u1ea7u c\u00f3 th\u1ec3 t\u1ed3n t\u1ea1i sau qu\u00e1 tr\u00ecnh ly gi\u1ea3i h\u1ed3ng c\u1ea7u.<\/p>\n<h3 style=\"text-align: justify;\"><strong>Th\u00e0nh ph\u1ea7n<\/strong>:<\/h3>\n<ul>\n<li>\n<p style=\"text-align: justify;\">0,8% NH4Cl<\/p>\n<\/li>\n<li>\n<p style=\"text-align: justify;\">0,1 mM EDTA pha trong n\u01b0\u1edbc, \u0111\u01b0\u1ee3c \u0111\u1ec7m b\u1eb1ng KHCO3 \u0111\u1ec3 \u0111\u1ea1t \u0111\u01b0\u1ee3c \u0111\u1ed9 pH cu\u1ed1i c\u00f9ng l\u00e0 7,2-7,6<\/p>\n<\/li>\n<\/ul>\n<h3 style=\"text-align: justify;\"><strong>H\u01b0\u1edbng d\u1eabn s\u1eed dung<\/strong>:<\/h3>\n<p style=\"text-align: justify;\">Chu\u1ea9n b\u1ecb ph\u1ea7n t\u1ebf b\u00e0o \u0111a nh\u00e2n trung t\u00ednh (Polymorphonuclear Cell) t\u1eeb m\u00e1u to\u00e0n ph\u1ea7n b\u1eb1ng c\u00e1ch ly gi\u1ea3i h\u1ed3ng c\u1ea7u s\u1eed d\u1ee5ng Ammonium Chloride<\/p>\n<ol>\n<li>\n<p style=\"text-align: justify;\">Thu th\u1eadp m\u00e1u to\u00e0n ph\u1ea7n v\u00e0o \u1ed1ng l\u1ea5y m\u00e1u c\u00f3 ch\u1ee9a heparin ho\u1eb7c m\u1ed9t ch\u1ea5t ch\u1ed1ng \u0111\u00f4ng m\u00e1u kh\u00e1c.<\/p>\n<\/li>\n<li>\n<p style=\"text-align: justify;\">Th\u00eam <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/nghien-cuu-sinh-hoc\/phan-lap-te-bao\/dung-dich-lymphoprep-ly-tam-ty-trong.html\">Lymphoprep\u2122<\/a> v\u00e0o \u1ed1ng. \u0110\u1ec3 bi\u1ebft kh\u1ed1i l\u01b0\u1ee3ng ph\u00f9 h\u1ee3p, vui l\u00f2ng xem b\u1ea3ng th\u00f4ng tin s\u1ea3n ph\u1ea9m.<\/p>\n<\/li>\n<li>\n<p style=\"text-align: justify;\">Pha lo\u00e3ng m\u00e1u to\u00e0n ph\u1ea7n t\u1ec9 l\u1ec7 1:1 <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/nuoi-cay-te-bao\/tang-sinh-te-bao-msc\/dung-dich-pbs.html\">Dulbecco&#8217;s Phosphate Buffered Saline<\/a> v\u1edbi 2% Fetal Bovine Serum (PBS + 2% FBS; Catalog #07905), ho\u1eb7c m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y th\u00edch h\u1ee3p kh\u00e1c.<\/p>\n<\/li>\n<li>\n<p style=\"text-align: justify;\">C\u1ea9n th\u1eadn cho l\u1edbp m\u00e1u l\u00ean tr\u00ean Lymphoprep\u2122, \u0111\u1ec3 gi\u1ea3m thi\u1ec3u s\u1ef1 tr\u1ed9n l\u1eabn m\u00e1u v\u1edbi Lymphoprep \u2122.<\/p>\n<\/li>\n<li>Ly t\u00e2m trong 20 ph\u00fat \u1edf 800 x g \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng (15-25\u00b0C), ng\u1eaft phanh.<img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/assets\/uploads\/image\/captureffd.JPG\" alt=\"\" width=\"300\" height=\"205\" \/><\/li>\n<li>\n<p style=\"text-align: justify;\">T\u00e1i huy\u1ec1n ph\u00f9 c\u00e1c t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u trong Dung d\u1ecbch Amoni Clorua (#07800\/07850) \u0111\u1ec3 ly gi\u1ea3i h\u1ed3ng c\u1ea7u. \u0110\u1ed5 \u0111\u1ea7y ho\u00e0n to\u00e0n v\u00e0o \u1ed1ng b\u1eb1ng dung d\u1ecbch ly gi\u1ea3i RBC v\u00e0 \u1ee7 tr\u00ean \u0111\u00e1 trong 10 ph\u00fat.Lo\u1ea1i b\u1ecf l\u1edbp huy\u1ebft t\u01b0\u01a1ng, d\u1ea3i t\u1ebf b\u00e0o \u0111\u01a1n nh\u00e2n v\u00e0 Lymphoprep \u2122, \u0111\u1ec3 l\u1ea1i l\u1edbp \u201cpellet\u2019 h\u1ed3ng c\u1ea7u (RBC)\/b\u1ea1ch c\u1ea7u h\u1ea1t. T\u00f9y ch\u1ecdn: T\u1ea1i th\u1eddi \u0111i\u1ec3m n\u00e0y, l\u1edbp \u201cpellet\u201d c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c chuy\u1ec3n sang m\u1ed9t \u1ed1ng m\u1edbi \u0111\u1ec3 tr\u00e1nh b\u1ecb nhi\u1ec5m b\u1edfi c\u00e1c t\u1ebf b\u00e0o \u0111\u01a1n nh\u00e2n c\u00f2n s\u00f3t l\u1ea1i trong \u1ed1ng.<\/p>\n<\/li>\n<li>\n<p style=\"text-align: justify;\">T\u00e1i huy\u1ec1n ph\u00f9 c\u00e1c t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u trong Dung d\u1ecbch Amoni Clorua (#07800\/07850) \u0111\u1ec3 ly gi\u1ea3i h\u1ed3ng c\u1ea7u. \u0110\u1ed5 \u0111\u1ea7y ho\u00e0n to\u00e0n v\u00e0o \u1ed1ng b\u1eb1ng dung d\u1ecbch ly gi\u1ea3i RBC v\u00e0 \u1ee7 tr\u00ean \u0111\u00e1 trong 10 ph\u00fat.<\/p>\n<\/li>\n<li>\n<p style=\"text-align: justify;\">Ly t\u00e2m trong 10 ph\u00fat \u1edf 500 x g \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng, v\u1edbi phanh \u0111\u1eb7t \u1edf m\u1ee9c th\u1ea5p.<\/p>\n<\/li>\n<li>\n<p style=\"text-align: justify;\">Lo\u1ea1i b\u1ecf ph\u1ea7n dung d\u1ecbch n\u1ed5i ph\u00eda tr\u00ean (Supernatant) v\u00e0 r\u1eeda ph\u1ea7n \u201cpellet\u201d trong PBS + 2% FBS \u0111\u1ec3 lo\u1ea1i b\u1ecf ti\u1ec3u c\u1ea7u, ly t\u00e2m trong 10 ph\u00fat \u1edf 120 x g \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng, t\u1eaft phanh.<\/p>\n<\/li>\n<li>\n<p style=\"text-align: justify;\">T\u00e1i huy\u1ec1n ph\u00f9 c\u00e1c t\u1ebf b\u00e0o \u0111a nh\u00e2n trung t\u00ednh (PMNCs) v\u1eeba thu \u0111\u01b0\u1ee3c trong m\u00f4i tr\u01b0\u1eddng th\u00edch h\u1ee3p (v\u00ed d\u1ee5: PBS + 2% FBS).<\/p>\n<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Dung d\u1ecbch Ammonium Chloride \u0111\u01b0\u1ee3c khuy\u1ebfn ngh\u1ecb d\u00f9ng \u0111\u1ec3 ly gi\u1ea3i t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u (RBCs) trong c\u00e1c ch\u1ebf ph\u1ea9m c\u1ee7a m\u00e1u ngo\u1ea1i vi ng\u01b0\u1eddi v\u00e0 chu\u1ed9t, l\u00e1 l\u00e1ch ho\u1eb7c t\u1ebf b\u00e0o t\u1ee7y x\u01b0\u01a1ng c\u1ee7a chu\u1ed9t. Ammonium Chloride \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng nh\u01b0 dung d\u1ecbch \u0111\u1ec7m, t\u1ed1i \u01b0u h\u00f3a \u0111\u1ec3 ly gi\u1ea3i h\u1ed3ng c\u1ea7u m\u1ed9t c\u00e1ch<\/p>\n<div class=\"klb-readmore post-buttons\"><a class=\"button rounded primary\" href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/dung-dich-ammonium-chloride\/\">Read More <i class=\"klbth-icon-right-arrow\"><\/i><\/a><\/div>\n","protected":false},"featured_media":10224,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[127],"product_cat":[115],"product_tag":[],"class_list":{"0":"post-3735","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-stemcell-technologies","7":"product_cat-phan-lap-te-bao","9":"first","10":"outofstock","11":"shipping-taxable","12":"product-type-simple"},"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - 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