M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd FPLC cho ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd \u0111u\u00f4i \u00e1i l\u1ef1c (Tag-Affinity) d\u1ef1a tr\u00ean c\u00e1c h\u1ea1t agarose li\u00ean k\u1ebft ch\u00e9o, v\u1edbi \u0111\u01b0\u1eddng k\u00ednh trung b\u00ecnh 100 \u03bcm. H\u1ea1t s\u1eafc k\u00fd \u0111\u01b0\u1ee3c g\u1eafn c\u00e1c ligand ch\u1ee9c n\u0103ng \u00e1i l\u1ef1c NTA ligands cho His-Tag ho\u1eb7c glutathione ligands cho GST-Tag.<\/p>\n
M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd Sepapure\u00ae Tag-Affinity \u1ee9ng d\u1ee5ng tinh s\u1ea1ch c\u00e1c protein t\u00e1i t\u1ed5 h\u1ee3p \u0111\u01b0\u1ee3c g\u1eafn \u0111u\u00f4i, \u0111\u01b0\u1ee3c s\u1ea3n xu\u1ea5t \u0111\u1ec3 d\u00f9ng v\u1edbi nhi\u1ec1u lo\u1ea1i dung d\u1ecbch \u0111\u1ec7m. M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd Sepapure\u00ae Tag-Affinity c\u00f3 h\u1ea1n s\u1eed d\u1ee5ng l\u00e2u d\u00e0i khi \u0111\u01b0\u1ee3c thao t\u00e1c v\u00e0 b\u1ea3o qu\u1ea3n \u0111\u00fang c\u00e1ch v\u00e0 t\u01b0\u01a1ng th\u00edch v\u1edbi h\u1ea7u h\u1ebft ph\u01b0\u01a1ng ph\u00e1p ti\u1ec7t tr\u00f9ng CIP. T\u1ea5t c\u1ea3 m\u00f4i tr\u01b0\u1eddng Sepapure\u00ae \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u1edf dung d\u1ecbch ethanol 20%.<\/p>\n
H\u1ea1t s\u1eafc k\u00fd Sepapure\u00ae Tag-Affinity c\u00f3 kh\u1ea3 n\u0103ng b\u00e1m (b\u1eaft protein) t\u1eeb 1-40 mg\/l (\u0111\u1ed1i v\u1edbi h\u1ea1t s\u1eafc k\u00fd Ni-NTA) ho\u1eb7c 1-10 mg\/ml (\u0111\u1ed1i v\u1edbi h\u1ea1t s\u1eafc k\u00fd Glutathione). \u00c1p su\u1ea5t v\u1eadn h\u00e0nh t\u1ed1i \u0111a cho c\u1ed9t Sepapure\u00ae n\u00ean s\u1eed d\u1ee5ng \u1edf \u00e1p su\u1ea5t 3 bar, t\u1ed1c \u0111\u1ed9 d\u00f2ng ch\u1ea3y khuy\u1ebfn ngh\u1ecb l\u00e0 1 CV\/ml.<\/p>\n
H\u1ea1t s\u1eafc k\u00fd Sepapure\u00ae Tag-Affinity th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u1edf b\u01b0\u1edbc \u201ccapture\u201d trong quy tr\u00ecnh tinh s\u1ea1ch protein.<\/p>\n
M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd FPLC cho ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd \u0111u\u00f4i \u00e1i l\u1ef1c (Tag-Affinity) d\u1ef1a tr\u00ean c\u00e1c h\u1ea1t agarose li\u00ean k\u1ebft ch\u00e9o, v\u1edbi \u0111\u01b0\u1eddng k\u00ednh trung b\u00ecnh 100 \u03bcm. H\u1ea1t s\u1eafc k\u00fd \u0111\u01b0\u1ee3c g\u1eafn c\u00e1c ligand ch\u1ee9c n\u0103ng \u00e1i l\u1ef1c NTA ligands cho His-Tag ho\u1eb7c glutathione ligands cho GST-Tag. \u0110\u1eb7c t\u00ednh M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd<\/p>\n