M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd FPLC cho ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd trao \u0111\u1ed5i ion d\u1ef1a tr\u00ean c\u00e1c h\u1ea1t agarose li\u00ean k\u1ebft ch\u00e9o, v\u1edbi \u0111\u01b0\u1eddng k\u00ednh trung b\u00ecnh 100 \u03bcm. H\u1ea1t s\u1eafc k\u00fd \u0111\u01b0\u1ee3c g\u1eafn c\u00e1c ligand ch\u1ee9c n\u0103ng trao \u0111\u1ed5i ion \u00e2m m\u1ea1nh ho\u1eb7c y\u1ebfu, c\u00e1c ligand ch\u1ee9c n\u0103ng trao \u0111\u1ed5i ion d\u01b0\u01a1ng m\u1ea1nh ho\u1eb7c y\u1ebfu.<\/p>\n
M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd trao \u0111\u1ed5i ion Sepapure\u00ae \u1ee9ng d\u1ee5ng tinh s\u1ea1ch c\u00e1c protein, \u0111\u01b0\u1ee3c s\u1ea3n xu\u1ea5t \u0111\u1ec3 d\u00f9ng v\u1edbi nhi\u1ec1u lo\u1ea1i dung d\u1ecbch \u0111\u1ec7m. M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd trao \u0111\u1ed5i ion Sepapure\u00ae c\u00f3 h\u1ea1n s\u1eed d\u1ee5ng l\u00e2u d\u00e0i khi \u0111\u01b0\u1ee3c thao t\u00e1c v\u00e0 b\u1ea3o qu\u1ea3n \u0111\u00fang c\u00e1ch v\u00e0 t\u01b0\u01a1ng th\u00edch v\u1edbi h\u1ea7u h\u1ebft ph\u01b0\u01a1ng ph\u00e1p ti\u1ec7t tr\u00f9ng CIP. T\u1ea5t c\u1ea3 m\u00f4i tr\u01b0\u1eddng Sepapure\u00ae \u0111\u01b0\u1ee3c l\u01b0u tr\u1eef \u1edf dung d\u1ecbch ethanol 20%.<\/p>\n
M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd trao \u0111\u1ed5i ion Sepapure\u00ae \u0111\u01b0\u1ee3c g\u1eafn v\u1edbi c\u00e1c ligand ch\u1ee9c n\u0103ng quaternary ammonium cho trao \u0111\u1ed5i anion m\u1ea1nh (Q), v\u00e0 v\u1edbi ligand diethylaminoethyl cho trao \u0111\u1ed5i anion y\u1ebfu (DEAE). S\u1ef1 trao \u0111\u1ed5i cation m\u1ea1nh \u0111\u01b0\u1ee3c g\u1eafn v\u1edbi c\u00e1c ligand ch\u1ee9c n\u0103ng sulphopropyl (SP), v\u00e0 trao \u0111\u1ed5i cation y\u1ebfu v\u1edbi gigand carboxymethyl (CM). \u00c1p su\u1ea5t v\u1eadn h\u00e0nh t\u1ed1i \u0111a cho c\u1ed9t Sepapure\u00ae n\u00ean s\u1eed d\u1ee5ng \u1edf \u00e1p su\u1ea5t 3 bar, t\u1ed1c \u0111\u1ed9 d\u00f2ng ch\u1ea3y khuy\u1ebfn ngh\u1ecb l\u00e0 1 CV\/ml.<\/p>\n
H\u1ea1t tr\u01b0\u1eddng s\u1eafc k\u00fd trao \u0111\u1ed5i ion Sepapure\u00ae th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u1edf b\u01b0\u1edbc trung gian (intermediate step) trong quy tr\u00ecnh tinh s\u1ea1ch protein<\/p>\n
M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd FPLC cho ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd trao \u0111\u1ed5i ion d\u1ef1a tr\u00ean c\u00e1c h\u1ea1t agarose li\u00ean k\u1ebft ch\u00e9o, v\u1edbi \u0111\u01b0\u1eddng k\u00ednh trung b\u00ecnh 100 \u03bcm. H\u1ea1t s\u1eafc k\u00fd \u0111\u01b0\u1ee3c g\u1eafn c\u00e1c ligand ch\u1ee9c n\u0103ng trao \u0111\u1ed5i ion \u00e2m m\u1ea1nh ho\u1eb7c y\u1ebfu, c\u00e1c ligand ch\u1ee9c n\u0103ng trao \u0111\u1ed5i ion d\u01b0\u01a1ng m\u1ea1nh ho\u1eb7c y\u1ebfu.<\/p>\n