M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd FPLC cho ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd l\u1ecdc gel d\u1ef1a tr\u00ean m\u1ed9t h\u1ed7n h\u1ee3p agarose-dextran li\u00ean k\u1ebft ch\u00e9o, v\u1edbi \u0111\u01b0\u1eddng k\u00ednh trung b\u00ecnh 35 \u03bcm.<\/p>\n
M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd l\u1ecdc gel Sepapure\u00ae \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf cho \u0111\u1ec3 tinh s\u1ea1ch c\u00e1c ph\u00e2n t\u1eed sinh h\u1ecdc d\u1ef1a tr\u00ean s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 k\u00edch th\u01b0\u1edbc ph\u00e2n t\u1eed c\u1ee7a ch\u00fang. M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd l\u1ecdc gel Sepapure\u00ae c\u00f3 h\u1ea1n s\u1eed d\u1ee5ng l\u00e2u d\u00e0i khi \u0111\u01b0\u1ee3c thao t\u00e1c v\u00e0 b\u1ea3o qu\u1ea3n \u0111\u00fang c\u00e1ch v\u00e0 t\u01b0\u01a1ng th\u00edch v\u1edbi h\u1ea7u h\u1ebft ph\u01b0\u01a1ng ph\u00e1p ti\u1ec7t tr\u00f9ng CIP. T\u1ea5t c\u1ea3 m\u00f4i tr\u01b0\u1eddng Sepapure\u00ae \u0111\u01b0\u1ee3c l\u01b0u tr\u1eef \u1edf dung d\u1ecbch ethanol 20%.<\/p>\n
H\u1ea1t s\u1eafc k\u00fd l\u1ecdc gel Sepapure\u00ae c\u00f3 kho\u1ea3ng ph\u00e2n \u0111o\u1ea1n t\u1eeb 3-70 kDa (h\u1ea1t s\u1eafc k\u00fd Sepapure\u00ae SEC 75) ho\u1eb7c 6 – 600 kDa (h\u1ea1t s\u1eafc k\u00fd Sepapure\u00ae SEC 200). \u00c1p su\u1ea5t v\u1eadn h\u00e0nh t\u1ed1i \u0111a cho c\u1ed9t Sepapure\u00ae n\u00ean s\u1eed d\u1ee5ng \u1edf \u00e1p su\u1ea5t 3 bar.<\/p>\n
H\u1ea1t tr\u01b0\u1eddng s\u1eafc k\u00fd l\u1ecdc gel Sepapure\u00ae th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u1edf b\u01b0\u1edbc cu\u1ed1i (final step) trong quy tr\u00ecnh tinh s\u1ea1ch protein.<\/p>\n
M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd FPLC cho ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd l\u1ecdc gel d\u1ef1a tr\u00ean m\u1ed9t h\u1ed7n h\u1ee3p agarose-dextran li\u00ean k\u1ebft ch\u00e9o, v\u1edbi \u0111\u01b0\u1eddng k\u00ednh trung b\u00ecnh 35 \u03bcm. \u0110\u1eb7c t\u00ednh M\u00f4i tr\u01b0\u1eddng s\u1eafc k\u00fd l\u1ecdc gel Sepapure\u00ae \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf cho \u0111\u1ec3 tinh s\u1ea1ch c\u00e1c ph\u00e2n t\u1eed sinh h\u1ecdc d\u1ef1a tr\u00ean s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1<\/p>\n