{"id":3778,"date":"2025-08-18T19:53:15","date_gmt":"2025-08-18T12:53:15","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/product\/import-placeholder-for-104\/"},"modified":"2026-02-09T10:08:05","modified_gmt":"2026-02-09T03:08:05","slug":"cellcor-msc-cd-aof","status":"publish","type":"product","link":"https:\/\/biogroupvietnam.com\/vi\/san-pham\/cellcor-msc-cd-aof\/","title":{"rendered":"M\u00f4i Tr\u01b0\u1eddng CellCor\u2122 CD MSC AOF &#8211; GMP"},"content":{"rendered":"<h3>Gi\u1edbi thi\u1ec7u CellCor\u2122 MSC CD AOF \u2013 M\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y hMSC \u201cChemically Defined\u201d, Animal-Origin-Free cho ph\u00f2ng lab chu\u1ea9n GMP<\/h3>\n<h4><strong>T\u1ea1i sao ch\u1ecdn CellCor\u2122 MSC CD AOF cho hMSC?<\/strong><\/h4>\n<ul>\n<li><strong>Chemically Defined &amp; Animal-Origin-Free (AOF):<\/strong> Kh\u00f4ng ch\u1ee9a th\u00e0nh ph\u1ea7n c\u00f3 ngu\u1ed3n g\u1ed1c \u0111\u1ed9ng v\u1eadt; tr\u00e1nh xenoantigen, gi\u1ea3m r\u1ee7i ro \u0111\u00e1p \u1ee9ng mi\u1ec5n d\u1ecbch v\u00e0 vi\u00eam.<\/li>\n<li><strong>Hi\u1ec7u n\u0103ng t\u0103ng sinh \u1ed5n \u0111\u1ecbnh qua nhi\u1ec1u passage:<\/strong> \u0110\u1ea3m b\u1ea3o k\u00edch th\u01b0\u1edbc t\u1ebf b\u00e0o, th\u1eddi gian nh\u00e2n \u0111\u00f4i (PDT) v\u00e0 t\u1ed5ng s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o (ACN) kh\u00f4ng thay \u0111\u1ed5i t\u1eeb P4 \u0111\u1ebfn P15<\/li>\n<li><strong>Ti\u1ebft ki\u1ec7m \u2013 kh\u00f4ng c\u1ea7n coating &amp; ph\u1ee5 gia:<\/strong> Kh\u00f4ng y\u00eau c\u1ea7u b\u1ed5 sung supplement hay ch\u1ea5t ph\u1ee7 b\u1ec1 m\u1eb7t; s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o thu \u0111\u01b0\u1ee3c cao trong \u0111i\u1ec1u ki\u1ec7n non-coating.<\/li>\n<li><strong>\u0110\u01a1n gi\u1ea3n ho\u00e1 quy tr\u00ecnh &amp; ki\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng:<\/strong> M\u00f4i tr\u01b0\u1eddng serum-free, th\u00e0nh ph\u1ea7n t\u1ed5ng h\u1ee3p\/t\u00e1i t\u1ed5 h\u1ee3p; \u0111\u00e3 ki\u1ec3m tra bioactivity, endotoxin, mycoplasma.<\/li>\n<\/ul>\n<h4><strong>\u1ee8ng d\u1ee5ng ti\u00eau bi\u1ec3u<\/strong><\/h4>\n<ul>\n<li>T\u0103ng sinh human MSC (hMSC) d\u00f9ng cho nghi\u00ean c\u1ee9u, ti\u1ec1n l\u00e2m s\u00e0ng v\u00e0 c\u00e1c workflow QC d\u01b0\u1edbi m\u00f4i tr\u01b0\u1eddng ki\u1ec3m so\u00e1t (R&amp;D \u2192 s\u1ea3n xu\u1ea5t theo chu\u1ea9n GMP).<\/li>\n<\/ul>\n<h4><strong>D\u1eef li\u1ec7u hi\u1ec7u n\u0103ng n\u1ed5i b\u1eadt<\/strong><\/h4>\n<ul>\n<li><strong>T\u0103ng sinh b\u1ec1n v\u1eefng:<\/strong> \u0110\u01b0\u1eddng cong ACN v\u00e0 PDT c\u1ee7a CellCor MSC CD AOF th\u1ec3 hi\u1ec7n s\u1ef1 \u1ed5n \u0111\u1ecbnh v\u00e0 \u01b0u th\u1ebf so v\u1edbi c\u00e1c m\u00f4i tr\u01b0\u1eddng \u0111\u1ed1i ch\u1ee9ng (FBS media, Media A) t\u1eeb P4\u2013P15.<\/li>\n<li><strong>\u0110\u1ed9 \u1ed5n \u0111\u1ecbnh &amp; mi\u1ec5n d\u1ecbch th\u1ea5p: <\/strong>Neu5Gc (xenoantigen) th\u1ea5p; CXCL12\/PTX3 (mi\u1ec5n d\u1ecbch\/vi\u00eam) v\u00e0 TNFRSF11B (vi\u00eam \u2013 protein) th\u1ea5p trong CellCor MSC CD AOF.<\/li>\n<li><strong>Kh\u00f4ng c\u1ea7n coating:<\/strong> So s\u00e1nh \u201cNon-coating\u201d v\u1edbi c\u00e1c l\u1edbp ph\u1ee7 (Fibronectin, Laminin, Vitronectin\u2026) cho th\u1ea5y CellCor MSC CD AOF v\u1eabn \u0111\u1ea1t s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o cao, gi\u00fap gi\u1ea3m chi ph\u00ed &amp; r\u1ee7i ro bi\u1ebfn thi\u00ean.<\/li>\n<\/ul>\n<h4><strong>Th\u00f4ng tin s\u1ea3n ph\u1ea9m CellCor\u2122 MSC CD AOF<\/strong><\/h4>\n<ul>\n<li><strong>T\u00ean s\u1ea3n ph\u1ea9m:<\/strong> CellCor\u2122 MSC CD AOF<\/li>\n<li><strong>Quy c\u00e1ch:<\/strong> 500 mL (Cat. No. YSP018)<\/li>\n<li><strong>\u0110\u1eb7c t\u00ednh:<\/strong> Kh\u00f4ng ch\u1ee9a huy\u1ebft thanh (Serum-free), th\u00e0nh ph\u1ea7n h\u00f3a h\u1ecdc x\u00e1c \u0111\u1ecbnh (CD), kh\u00f4ng c\u00f3 ngu\u1ed3n g\u1ed1c \u0111\u1ed9ng v\u1eadt (AOF); kh\u00f4ng c\u1ea7n b\u1ed5 sung ch\u1ea5t ph\u1ee5 gia hay ch\u1ea5t ph\u1ee7 b\u1ec1 m\u1eb7t.<\/li>\n<li><strong>B\u1ea3o qu\u1ea3n:<\/strong> \u2264 \u221220 \u00b0C<\/li>\n<li><strong>HSD:<\/strong> 12 th\u00e1ng.<\/li>\n<li><strong>\u0110\u00e3 ki\u1ec3m tra ch\u1ea5t l\u01b0\u1ee3ng:<\/strong> Bioactivity, endotoxin, mycoplasma\u2026<\/li>\n<li><strong>\u1ee8ng d\u1ee5ng:<\/strong> T\u0103ng sinh t\u1ebf b\u00e0o g\u1ed1c trung m\u00f4 ng\u01b0\u1eddi (hMSC)<\/li>\n<\/ul>\n<h4><strong>H\u01b0\u1edbng d\u1eabn s\u1eed d\u1ee5ng nhanh (khuy\u1ebfn ngh\u1ecb k\u1ef9 thu\u1eadt)<\/strong><\/h4>\n<ul>\n<li><strong>M\u1eadt \u0111\u1ed9 gieo (seeding):<\/strong> 3.000\u20134.000 cells\/cm\u00b2; thu ho\u1ea1ch \u1edf 75\u201385% confluency.<\/li>\n<li><strong>T\u00e1ch b\u00e1m:<\/strong> \u01afu ti\u00ean TrypLE\u2122 Express (kh\u00f4ng c\u1ea7n trung ho\u00e0 b\u1eb1ng serum); kh\u00f4ng khuy\u1ebfn ngh\u1ecb Trypsin-EDTA do m\u00f4i tr\u01b0\u1eddng kh\u00f4ng ch\u1ee9a serum.<\/li>\n<li><strong>D\u1ee5ng c\u1ee5 nu\u00f4i c\u1ea5y:<\/strong> Flask\/Plate\/Cell Factory chu\u1ea9n TC (Corning, Nunc, Falcon). Kh\u00f4ng c\u1ea7n coating v\u1edbi flask chu\u1ea9n.<\/li>\n<li><strong>R\u00e3 \u0111\u00f4ng &amp; thao t\u00e1c:<\/strong> R\u00e3 \u0111\u00f4ng, \u1ea5m 37 \u00b0C; d\u00f9ng ngay sau r\u00e3 \u0111\u00f4ng, tr\u00e1nh \u0111\u00f4ng-r\u00e3 nhi\u1ec1u l\u1ea7n. N\u1ebfu chia aliquot, c\u00f3 th\u1ec3 d\u00f9ng t\u1ed1i \u0111a 4 tu\u1ea7n \u1edf 4 \u00b0C trong th\u1eddi h\u1ea1n s\u1ea3n ph\u1ea9m.<\/li>\n<li><strong>Th\u00edch nghi (t\u00f9y ch\u1ecdn):<\/strong> Sau 24 h, thay 50% m\u00f4i tr\u01b0\u1eddng c\u0169 b\u1eb1ng CellCor MSC CD AOF; khi \u0111\u1ea1t 75\u201385% confluence, subculture ho\u00e0n to\u00e0n v\u1edbi CellCor MSC CD AOF.<\/li>\n<\/ul>\n<h4><strong>L\u1ee3i \u00edch v\u1eadn h\u00e0nh cho ph\u00f2ng lab &amp; nh\u00e0 s\u1ea3n xu\u1ea5t<\/strong><\/h4>\n<ul>\n<li><strong>Gi\u1ea3m bi\u1ebfn thi\u00ean l\u00f4 \u2013 d\u1ec5 chu\u1ea9n ho\u00e1 SOP:<\/strong> M\u00f4i tr\u01b0\u1eddng defined\/AOF gi\u00fap chu\u1ea9n ho\u00e1 d\u1eef li\u1ec7u t\u0103ng sinh, ph\u00f9 h\u1ee3p \u0111\u00e1nh gi\u00e1 PDT, ACN, k\u00edch th\u01b0\u1edbc t\u1ebf b\u00e0o trong c\u00e1c ch\u01b0\u01a1ng tr\u00ecnh ki\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng.<\/li>\n<li><strong>Ph\u00f9 h\u1ee3p ti\u00eau chu\u1ea9n GMP\/QA:<\/strong> Kh\u00f4ng nguy\u00ean li\u1ec7u ngu\u1ed3n g\u1ed1c \u0111\u1ed9ng v\u1eadt, \u0111\u00e3 ki\u1ec3m tra t\u1ea1p nhi\u1ec5m \u0111i\u1ec3n h\u00ecnh (endotoxin, mycoplasma).<\/li>\n<li><strong>T\u1ed1i \u01b0u chi ph\u00ed &amp; th\u1eddi gian:<\/strong> B\u1ecf qua b\u01b0\u1edbc ph\u1ee7 b\u1ec1 m\u1eb7t (non-coating), gi\u1ea3m v\u1eadt t\u01b0 &amp; thao t\u00e1c; duy tr\u00ec s\u1ed1 l\u01b0\u1ee3ng v\u00e0 h\u00ecnh th\u00e1i t\u1ebf b\u00e0o \u1ed5n \u0111\u1ecbnh.<\/li>\n<\/ul>\n<div id=\"attachment_9776\" style=\"width: 1045px\" class=\"wp-caption aligncenter\"><img fetchpriority=\"high\" decoding=\"async\" aria-describedby=\"caption-attachment-9776\" class=\"wp-image-9776\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/cellcor-msc-cd-aof-bo-qua-buoc-phu-be-mat-non-coating-giup-toi-uu-chi-phi-thoi-gian.png\" alt=\"CellCor\u2122 MSC CD AOF b\u1ecf qua b\u01b0\u1edbc ph\u1ee7 b\u1ec1 m\u1eb7t (non-coating) gi\u00fap t\u1ed1i \u01b0u chi ph\u00ed v\u00e0 th\u1eddi gian. (Ngu\u1ed3n: CellCor\u2122 MSC CD AOF Brochure)\" width=\"1035\" height=\"306\" srcset=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/cellcor-msc-cd-aof-bo-qua-buoc-phu-be-mat-non-coating-giup-toi-uu-chi-phi-thoi-gian.png 1351w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/cellcor-msc-cd-aof-bo-qua-buoc-phu-be-mat-non-coating-giup-toi-uu-chi-phi-thoi-gian-300x89.png 300w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/cellcor-msc-cd-aof-bo-qua-buoc-phu-be-mat-non-coating-giup-toi-uu-chi-phi-thoi-gian-1024x303.png 1024w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/cellcor-msc-cd-aof-bo-qua-buoc-phu-be-mat-non-coating-giup-toi-uu-chi-phi-thoi-gian-768x227.png 768w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/cellcor-msc-cd-aof-bo-qua-buoc-phu-be-mat-non-coating-giup-toi-uu-chi-phi-thoi-gian-600x178.png 600w, https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/08\/cellcor-msc-cd-aof-bo-qua-buoc-phu-be-mat-non-coating-giup-toi-uu-chi-phi-thoi-gian-90x27.png 90w\" sizes=\"(max-width: 1035px) 100vw, 1035px\" \/><p id=\"caption-attachment-9776\" class=\"wp-caption-text\"><em>CellCor\u2122 MSC CD AOF b\u1ecf qua b\u01b0\u1edbc ph\u1ee7 b\u1ec1 m\u1eb7t (non-coating) gi\u00fap t\u1ed1i \u01b0u chi ph\u00ed v\u00e0 th\u1eddi gian. (Ngu\u1ed3n: CellCor\u2122 MSC CD AOF Brochure)<\/em><\/p><\/div>\n<h4><strong>C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p (FAQ)<\/strong><\/h4>\n<p><strong>1) C\u00f3 c\u1ea7n coating flask\/plate?<\/strong><br \/>\nKh\u00f4ng. D\u00f9ng flask chu\u1ea9n TC l\u00e0 \u0111\u1ee7; d\u1eef li\u1ec7u non-coating cho th\u1ea5y s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o thu \u0111\u01b0\u1ee3c cao.<\/p>\n<p><strong>2) V\u00ec sao n\u00ean d\u00f9ng TrypLE\u2122 Express?<\/strong><br \/>\nCellCor MSC CD AOF kh\u00f4ng c\u00f3 serum \u0111\u1ec3 trung ho\u00e0 trypsin; TrypLE\u2122 gi\u00fap h\u1ea1n ch\u1ebf t\u1ed5n th\u01b0\u01a1ng t\u1ebf b\u00e0o v\u00e0 kh\u00f4ng c\u1ea7n b\u01b0\u1edbc trung ho\u00e0.<\/p>\n<p><strong>3) B\u1ea3o qu\u1ea3n sau r\u00e3 \u0111\u00f4ng nh\u01b0 th\u1ebf n\u00e0o?<\/strong><br \/>\nTr\u00e1nh \u0111\u00f4ng-r\u00e3 l\u1eb7p l\u1ea1i; n\u1ebfu chia aliquot, c\u00f3 th\u1ec3 d\u00f9ng \u22644 tu\u1ea7n \u1edf 4 \u00b0C trong h\u1ea1n d\u00f9ng.<\/p>\n<p><strong>4) C\u00f3 c\u1ea7n b\u01b0\u1edbc th\u00edch nghi khi chuy\u1ec3n t\u1eeb m\u00f4i tr\u01b0\u1eddng kh\u00e1c sang?<\/strong><br \/>\nKhuy\u1ebfn ngh\u1ecb th\u00edch nghi 1 chu k\u1ef3 (50% c\u0169 + 50% CellCor) \u0111\u1ec3 \u1ed5n \u0111\u1ecbnh b\u00e1m d\u00ednh v\u00e0 t\u0103ng sinh trong \u0111i\u1ec1u ki\u1ec7n serum-free\/defined.<\/p>\n<h4><strong>Th\u00f4ng s\u1ed1 \u0111\u1eb7t h\u00e0ng<\/strong><\/h4>\n<ul>\n<li>CellCor\u2122 MSC CD AOF<\/li>\n<li>500 mL \u2013 Cat. No. YSP018 (RUO) &amp; YSP023 (GMP)<\/li>\n<li>B\u1ea3o qu\u1ea3n \u2264 \u221220 \u00b0C; HSD 12 th\u00e1ng; kh\u00f4ng c\u1ea7n supplement\/coating.<\/li>\n<\/ul>\n<p>Li\u00ean h\u1ec7 Biogroup Vietnam \u0111\u1ec3 \u0111\u01b0\u1ee3c t\u01b0 v\u1ea5n quy tr\u00ecnh nu\u00f4i c\u1ea5y hMSC, x\u00e2y d\u1ef1ng SOP IQ\/OQ\/PQ cho thi\u1ebft b\u1ecb li\u00ean quan (CO\u2082 incubator, t\u1ee7 an to\u00e0n sinh h\u1ecdc, m\u00e1y ly t\u00e2m, b\u00ecnh LN\u2082\/MVE\u2026), v\u00e0 gi\u1ea3i ph\u00e1p QC t\u1ebf b\u00e0o theo chu\u1ea9n cGMP\/AABB.<\/p>\n<h4><strong>G\u1ee3i \u00fd s\u1ea3n ph\u1ea9m li\u00ean quan (bundling theo workflow)<\/strong><\/h4>\n<ul>\n<li>T\u1ee7 \u1ea5m CO\u2082 NuAire, m\u00e1y ly t\u00e2m Hettich, b\u00ecnh LN\u2082 MVE, v\u1eadt t\u01b0 QC (CFU, mycoplasma test) \u2013 t\u1ed1i \u01b0u ho\u00e1 t\u0103ng sinh &amp; ki\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng hMSC trong chu tr\u00ecnh R&amp;D \u2192 s\u1ea3n xu\u1ea5t.<\/li>\n<\/ul>\n<h4><strong>T\u00e0i li\u1ec7u<\/strong><\/h4>\n<ul>\n<li><em>Brochure CellCor\u2122 MSC CD AOF<\/em> \u2013 d\u1eef li\u1ec7u hi\u1ec7u n\u0103ng, AOF\/ACF \u0111\u1ecbnh ngh\u0129a, th\u00f4ng tin kh\u00f4ng c\u1ea7n coating &amp; th\u00f4ng s\u1ed1 \u0111\u1eb7t h\u00e0ng.<\/li>\n<li><em>User Guide CellCor\u2122 MSC CD AOF<\/em> \u2013 m\u00f4 t\u1ea3 s\u1ea3n ph\u1ea9m, \u0111i\u1ec1u ki\u1ec7n nu\u00f4i c\u1ea5y khuy\u1ebfn ngh\u1ecb, FAQ, b\u1ea3o qu\u1ea3n &amp; thao t\u00e1c.<\/li>\n<\/ul>\n<p>H\u00e3y li\u00ean h\u1ec7 v\u1edbi Biogroup Vietnam khi b\u1ea1n c\u1ea7n t\u01b0 v\u1ea5n hay h\u1ed7 tr\u1ee3 th\u00eam th\u00f4ng tin s\u1ea3n ph\u1ea9m nh\u00e9!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CellCor\u2122 MSC CD AOF l\u00e0 m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y <strong data-start=\"73\" data-end=\"127\">serum-free, chemically defined, animal-origin-free<\/strong> d\u00e0nh cho t\u0103ng sinh hMSC t\u1eeb R&amp;D \u0111\u1ebfn quy tr\u00ecnh ph\u00f9 h\u1ee3p GMP. C\u00f4ng th\u1ee9c AOF gi\u00fap gi\u1ea3m r\u1ee7i ro xenoantigen (Neu5Gc) v\u00e0 t\u00edn hi\u1ec7u vi\u00eam, cho t\u0103ng sinh \u1ed5n \u0111\u1ecbnh qua nhi\u1ec1u passage v\u1edbi PDT ((Population Doubling Time), k\u00edch th\u01b0\u1edbc v\u00e0 h\u00ecnh th\u00e1i \u0111\u01b0\u1ee3c ki\u1ec3m so\u00e1t. S\u1ea3n ph\u1ea9m <strong data-start=\"352\" data-end=\"373\">kh\u00f4ng c\u1ea7n coating<\/strong> hay b\u1ed5 sung supplement, r\u00fat ng\u1eafn thao t\u00e1c, gi\u1ea3m bi\u1ebfn thi\u00ean l\u00f4, t\u1ed1i \u01b0u chi ph\u00ed. B\u1ea3o qu\u1ea3n \u2264 \u221220 \u00b0C; \u0111\u00e3 ki\u1ec3m tra bioactivity, endotoxin, mycoplasma. L\u00fd t\u01b0\u1edfng cho QC, ng\u00e2n h\u00e0ng t\u1ebf b\u00e0o v\u00e0 s\u1ea3n xu\u1ea5t li\u1ec7u ph\u00e1p t\u1ebf b\u00e0o.<\/p>\n","protected":false},"featured_media":9026,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[132],"product_cat":[91],"product_tag":[],"class_list":{"0":"post-3778","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-xcell-therapeutics-inc","7":"product_cat-te-bao-goc-trung-mo","9":"first","10":"outofstock","11":"shipping-taxable","12":"product-type-simple"},"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>M\u00f4i tr\u01b0\u1eddng CellCor MSC CD AOF<\/title>\n<meta name=\"description\" content=\"M\u00f4i tr\u01b0\u1eddng CellCor\u2122 MSC CD AOF: serum-free, chemically defined, AOF; t\u0103ng sinh hMSC \u1ed5n \u0111\u1ecbnh, kh\u00f4ng c\u1ea7n coating, l\u01b0u tr\u1eef \u221220\u00b0C. 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