{"id":3933,"date":"2025-08-18T19:54:44","date_gmt":"2025-08-18T12:54:44","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/product\/import-placeholder-for-188\/"},"modified":"2026-04-02T11:46:48","modified_gmt":"2026-04-02T04:46:48","slug":"extracellular-vesicle-sec-columns","status":"publish","type":"product","link":"https:\/\/biogroupvietnam.com\/vi\/san-pham\/extracellular-vesicle-sec-columns\/","title":{"rendered":"C\u1ed9t SEC Ph\u00e2n L\u1eadp T\u00fai Ngo\u1ea1i B\u00e0o"},"content":{"rendered":"<h2>C\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC) \u0111\u1ec3 ph\u00e2n l\u1eadp c\u00e1c t\u00fai ngo\u1ea1i b\u00e0o (EV) t\u1eeb c\u00e1c ch\u1ea5t l\u1ecfng sinh h\u1ecdc<\/h2>\n<h3><strong>T\u1ed5ng quan v\u1ec1 c\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc<\/strong><\/h3>\n<p>C\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (Size exclusion chromatography &#8211; SEC) \u0111\u1ec3 ph\u00e2n l\u1eadp c\u00e1c t\u00fai ngo\u1ea1i b\u00e0o (Extracellular Vesicle &#8211; EV) t\u1eeb c\u00e1c ch\u1ea5t l\u1ecfng sinh h\u1ecdc, bao g\u1ed3m huy\u1ebft t\u01b0\u01a1ng, huy\u1ebft thanh, m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o v\u00e0 cung c\u1ea5p m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p hi\u1ec7u qu\u1ea3 \u0111\u1ec3 t\u00e1ch EV kh\u1ecfi protein tu\u1ea7n ho\u00e0n. So v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n l\u1eadp EV kh\u00e1c, c\u1ed9t SEC g\u00e2y ra s\u1ef1 thay \u0111\u1ed5i t\u1ed1i thi\u1ec3u v\u00ea c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m nh\u01b0 ch\u1ee9c n\u0103ng v\u00e0 h\u00ecnh d\u1ea1ng c\u1ee7a t\u00fai EV.<\/p>\n<p>Quy tr\u00ecnh ph\u00e2n l\u1eadp s\u1eed d\u1ee5ng c\u1ed9t SEC r\u1ea5t d\u1ec5 d\u00e0ng v\u00e0 m\u1ea5t kho\u1ea3ng 15 ph\u00fat.<\/p>\n<p>C\u00e1c c\u1ed9t c\u00f3 s\u1eb5n ba k\u00edch c\u1ee1 cho c\u00e1c th\u1ec3 t\u00edch m\u1eabu x\u1eed l\u00fd kh\u00e1c nhau v\u00e0 c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng t\u1ed1i \u0111a n\u0103m l\u1ea7n.<\/p>\n<h3><strong>\u01afu \u0111i\u1ec3m c\u1ee7a c\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb\u00a0k\u00edch th\u01b0\u1edbc<\/strong><\/h3>\n<ul>\n<li>Quy tr\u00ecnh nhanh ch\u00f3ng, d\u1ec5 s\u1eed d\u1ee5ng<\/li>\n<li>C\u00f3 th\u1ec3 t\u00e1i s\u1eed d\u1ee5ng (t\u1ed1i \u0111a n\u0103m l\u1ea7n)<\/li>\n<\/ul>\n<h3><strong>B\u1ea3o qu\u1ea3n<\/strong><\/h3>\n<ul>\n<li>B\u1ea3o qu\u1ea3n \u1edf nhi\u1ec7t \u0111\u1ed9 2-8\u00b0C.<\/li>\n<\/ul>\n<h3><strong>\u1ee8ng d\u1ee5ng<\/strong><\/h3>\n<p>C\u00e1c EV \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp th\u00edch h\u1ee3p cho c\u00e1c \u1ee9ng d\u1ee5ng ph\u00e2n t\u00edch ti\u1ebfp theo bao g\u1ed3m ph\u01b0\u01a1ng ph\u00e1p \u0111o d\u00f2ng ch\u1ea3y t\u1ebf b\u00e0o, Western blot, chi\u1ebft xu\u1ea5t axit nucleic v\u00e0 c\u00e1c x\u00e9t nghi\u1ec7m ch\u1ee9c n\u0103ng.<\/p>\n<h3><strong>Th\u00f4ng tin \u0111\u1eb7t h\u00e0ng:<\/strong><\/h3>\n<p>C\u00e1c c\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc t\u00fai ngo\u1ea1i b\u00e0o (SEC) c\u00f3 s\u1eb5n ba k\u00edch th\u01b0\u1edbc t\u00f9y ch\u1ecdn cho c\u00e1c th\u1ec3 t\u00edch m\u1eabu x\u1eed l\u00fd kh\u00e1c nhau:<\/p>\n<p>\u2022 C\u1ed9t SEC t\u00fai ngo\u1ea1i b\u00e0o 20 mL, Catalog #100-0416: 3 c\u1ed9t<\/p>\n<p>\u2022 C\u1ed9t SEC t\u00fai ngo\u1ea1i b\u00e0o 2 mL, Catalog #100-0415: 5 c\u1ed9t<\/p>\n<p>\u2022 C\u1ed9t SEC t\u00fai ngo\u1ea1i b\u00e0o 0,5 mL, Catalog #100-0414: 10 c\u1ed9t<\/p>\n<h3><strong>S\u1ea3n ph\u1ea9m y\u00eau c\u1ea7u b\u1ed5 sung nh\u01b0ng kh\u00f4ng bao g\u1ed3m<\/strong>:<\/h3>\n<figure class=\"table\">\n<table>\n<tbody>\n<tr>\n<td><strong>T\u00ean s\u1ea3n ph\u1ea9m<\/strong><\/td>\n<td><strong>Catalog #<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Dung d\u1ecbch D-PBS (Kh\u00f4ng c\u00f3 Ca++ v\u00e0 Mg++)<\/td>\n<td>37350<\/td>\n<\/tr>\n<tr>\n<td>\u1ed0ng si\u00eau ly t\u00e2m Costar\u00ae, 1,7 mL<\/td>\n<td>38089<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<h3><strong>Quy tr\u00ecnh ph\u00e2n l\u1eadp t\u00fai ngo\u1ea1i b\u00e0o v\u1edbi c\u1ed9t s\u1eafc k\u00fd SEC<\/strong><\/h3>\n<h3 style=\"text-align: center;\"><img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/uploads\/n0X16odtq35PDHdVHmprrkXcgUJuM1HdVT67c30e.png\" alt=\"\" width=\"754\" height=\"281\" \/><\/h3>\n<p>\u0110\u1ecdc th\u00eam v\u1ec1 quy tr\u00ecnh t\u1ea1i <a href=\"https:\/\/biogroupvietnam.com\/vi\/services\/quy-trinh-huong-dan-su-dung\/huong-dan-su-dung-cot-sac-ky-loai-tru-kich-thuoc-sec.html\">link<\/a>.<\/p>\n<h3><strong>D\u1eef li\u1ec7u<\/strong><\/h3>\n<h3 style=\"text-align: center;\"><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/uploads\/4XdjIZSaEVJIS1U0dIJLvvfO2VjOQWOOAA4cDnAV.png\" alt=\"\" width=\"652\" height=\"315\" \/><\/h3>\n<p style=\"text-align: center;\"><i><strong>H\u00ecnh 1: Ph\u00e2n l\u1eadp t\u00fai ngo\u1ea1i b\u00e0o t\u1eeb huy\u1ebft t\u01b0\u01a1ng b\u1eb1ng c\u1ed9t SEC 0,5 mL<\/strong><\/i><\/p>\n<p>C\u1ed9t SEC t\u00fai ngo\u1ea1i b\u00e0o 0,5 mL \u0111\u01b0\u1ee3c n\u1ea1p 0,5 mL huy\u1ebft t\u01b0\u01a1ng c\u1ee7a ng\u01b0\u1eddi.\u00a0C\u00e1c ph\u1ea7n 100 \u03bcL \u0111\u01b0\u1ee3c thu th\u1eadp v\u00e0 ph\u00e2n t\u00edch h\u00e0m l\u01b0\u1ee3ng h\u1ea1t v\u00e0 protein b\u1eb1ng c\u00e1c x\u00e9t nghi\u1ec7m ph\u00e2n t\u00edch theo d\u00f5i h\u1ea1t nano (NTA) v\u00e0 axit bicinchoninic (BCA), t\u01b0\u01a1ng \u1ee9ng.\u00a0EV \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n \u1edf gi\u1ecdt s\u1ed1 9 &#8211; 14, trong khi protein \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n \u1edf gi\u1ecdt 15 tr\u1edf \u0111i.<\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/uploads\/qjfm6QKWX282ZgcdUolwEAkH3K5KhpbyQnUqMgjG.png\" alt=\"\" width=\"653\" height=\"386\" \/><\/p>\n<p style=\"text-align: center;\"><i><strong>H\u00ecnh 2: Ph\u00e2n l\u1eadp c\u00e1c t\u00fai ngo\u1ea1i b\u00e0o t\u1eeb huy\u1ebft t\u01b0\u01a1ng b\u1eb1ng c\u1ed9t SEC 2 mL<\/strong><\/i><\/p>\n<p>C\u1ed9t SEC t\u00fai ngo\u1ea1i b\u00e0o 2 mL \u0111\u01b0\u1ee3c n\u1ea1p 2 mL huy\u1ebft t\u01b0\u01a1ng c\u1ee7a ng\u01b0\u1eddi.<\/p>\n<p>C\u00e1c ph\u1ea7n 500 \u03bcL \u0111\u01b0\u1ee3c thu th\u1eadp v\u00e0 ph\u00e2n t\u00edch h\u00e0m l\u01b0\u1ee3ng h\u1ea1t v\u00e0 protein b\u1eb1ng c\u00e1ch ph\u00e2n t\u00edch theo d\u00f5i h\u1ea1t nano v\u00e0 x\u00e9t nghi\u1ec7m axit bicinchoninic t\u01b0\u01a1ng \u1ee9ng.\u00a0EV \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n \u1edf gi\u1ecdt s\u1ed1 7 &#8211; 10, trong khi protein \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n \u1edf gi\u1ecdt 11 tr\u1edf \u0111i.<\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/uploads\/0DX3SGXMkruqkqAQWL5RcDgiJTLuwNYEzYYHGWGu.png\" alt=\"\" width=\"653\" height=\"376\" \/><\/p>\n<p style=\"text-align: center;\"><i><strong>H\u00ecnh 3.\u00a0Ph\u00e2n l\u1eadp c\u00e1c t\u00fai ngo\u1ea1i b\u00e0o t\u1eeb m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y b\u1eb1ng c\u1ed9t SEC 20 mL<\/strong><\/i><\/p>\n<p>C\u1ed9t SEC t\u00fai ngo\u1ea1i b\u00e0o 20 mL \u0111\u01b0\u1ee3c n\u1ea1p 20 mL m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o g\u1ed1c tr\u00f9ng m\u00f4 (MSC) kh\u00f4ng ch\u1ee9a huy\u1ebft thanh, c\u00f4 \u0111\u1eb7c g\u1ea5p 10 l\u1ea7n.\u00a0C\u00e1c gi\u1ecdt 1 mL \u0111\u01b0\u1ee3c thu th\u1eadp v\u00e0 ph\u00e2n t\u00edch h\u00e0m l\u01b0\u1ee3ng h\u1ea1t v\u00e0 protein b\u1eb1ng c\u00e1ch ph\u00e2n t\u00edch theo d\u00f5i h\u1ea1t nano v\u00e0 x\u00e9t nghi\u1ec7m axit bicinchoninic t\u01b0\u01a1ng \u1ee9ng.<\/p>\n<p>EV \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n \u1edf gi\u1ecdt s\u1ed1 15 &#8211; 30, trong khi protein \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n \u1edf gi\u1ecdt s\u1ed1 35 tr\u1edf \u0111i.\u00a0C\u00e1c ph\u1ea7n 31-33, n\u1eb1m ngo\u00e0i ph\u1ea1m vi ph\u00e2n l\u1eadp EV \u0111i\u1ec3n h\u00ecnh tuy nhi\u00ean v\u1eabn c\u00f3 th\u1ec3 ch\u1ee9a EV c\u00f3 m\u1ee9c \u0111\u1ed9 nhi\u1ec5m protein th\u1ea5p \u0111\u1ed1i v\u1edbi c\u00e1c m\u1eabu m\u00f4i tr\u01b0\u1eddng kh\u00f4ng c\u00f3 huy\u1ebft thanh.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>C\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC) \u0111\u1ec3 ph\u00e2n l\u1eadp c\u00e1c t\u00fai ngo\u1ea1i b\u00e0o (EV) t\u1eeb c\u00e1c ch\u1ea5t l\u1ecfng sinh h\u1ecdc T\u1ed5ng quan v\u1ec1 c\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc C\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (Size exclusion chromatography &#8211; SEC) \u0111\u1ec3 ph\u00e2n l\u1eadp c\u00e1c t\u00fai ngo\u1ea1i b\u00e0o (Extracellular Vesicle &#8211; EV)<\/p>\n<div class=\"klb-readmore post-buttons\"><a class=\"button rounded primary\" href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/extracellular-vesicle-sec-columns\/\">Read More <i class=\"klbth-icon-right-arrow\"><\/i><\/a><\/div>\n","protected":false},"featured_media":10143,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[127],"product_cat":[115],"product_tag":[],"class_list":{"0":"post-3933","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-stemcell-technologies","7":"product_cat-phan-lap-te-bao","9":"first","10":"outofstock","11":"shipping-taxable","12":"product-type-simple"},"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>C\u1ed9t SEC Ph\u00e2n L\u1eadp T\u00fai Ngo\u1ea1i B\u00e0o<\/title>\n<meta name=\"description\" content=\"C\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC) \u0111\u1ec3 ph\u00e2n l\u1eadp c\u00e1c t\u00fai ngo\u1ea1i b\u00e0o (EV) t\u1eeb c\u00e1c ch\u1ea5t l\u1ecfng sinh h\u1ecdc\" \/>\n<meta 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