{"id":4072,"date":"2025-08-18T19:56:10","date_gmt":"2025-08-18T12:56:10","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/product\/import-placeholder-for-264\/"},"modified":"2026-03-30T16:42:36","modified_gmt":"2026-03-30T09:42:36","slug":"bo-moi-truong-biet-hoa-organoid-ruot-intesticult-nguoi","status":"publish","type":"product","link":"https:\/\/biogroupvietnam.com\/vi\/san-pham\/bo-moi-truong-biet-hoa-organoid-ruot-intesticult-nguoi\/","title":{"rendered":"B\u1ed9 M\u00f4i Tr\u01b0\u1eddng Bi\u1ec7t H\u00f3a Organoid Ru\u1ed9t IntestiCult\u2122 (Ng\u01b0\u1eddi)"},"content":{"rendered":"<h3 style=\"text-align: justify;\"><strong>T\u1ed5ng quan v\u1ec1 b\u1ed9 m\u00f4i tr\u01b0\u1eddng bi\u1ec7t h\u00f3a organoid ru\u1ed9t IntestiCult\u2122<\/strong><\/h3>\n<p style=\"text-align: justify;\">B\u1ed9 M\u00f4i Tr\u01b0\u1eddng Bi\u1ec7t H\u00f3a Organoid Ru\u1ed9t IntestiCult\u2122 (Ng\u01b0\u1eddi) l\u00e0 m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y ho\u00e0n ch\u1ec9nh h\u1ed7 tr\u1ee3 bi\u1ec7t h\u00f3a th\u00eam c\u00e1c c\u01a1 quan ru\u1ed9t \u1edf ba chi\u1ec1u (3D) ho\u1eb7c \u1edf 2D d\u01b0\u1edbi d\u1ea1ng c\u00e1c l\u1edbp \u0111\u01a1n ho\u1eb7c nu\u00f4i c\u1ea5y b\u1ec1 m\u1eb7t kh\u00f4ng kh\u00ed-l\u1ecfng (ALI). C\u00e1c nu\u00f4i c\u1ea5y ban \u0111\u1ea7u c\u00f3 th\u1ec3 l\u00e0 c\u00e1c organoid ru\u1ed9t c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb c\u00e1c h\u1ed1c ru\u1ed9t ng\u01b0\u1eddi ho\u1eb7c c\u00e1c organoid \u0111\u00e3 \u0111\u01b0\u1ee3c chuy\u1ec3n gen \u0111\u00e3 \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y b\u1eb1ng M\u00f4i tr\u01b0\u1eddng t\u0103ng tr\u01b0\u1edfng organoid IntestiCult\u2122 (Ng\u01b0\u1eddi; Catalog #06010).<\/p>\n<p style=\"text-align: justify;\">C\u00e1c m\u1eabu nu\u00f4i c\u1ea5y ru\u1ed9t \u0111\u01b0\u1ee3c t\u1ea1o ra b\u1eb1ng M\u00f4i tr\u01b0\u1eddng bi\u1ec7t h\u00f3a organoid IntestiCult\u2122 (Ng\u01b0\u1eddi) ch\u1ee9a c\u00e1c t\u1ef7 l\u1ec7 li\u00ean quan v\u1ec1 m\u1eb7t sinh l\u00fd c\u1ee7a c\u00e1c qu\u1ea7n th\u1ec3 t\u1ebf b\u00e0o g\u1ed1c v\u00e0 bi\u1ec7t h\u00f3a, t\u00e1i hi\u1ec7n s\u1ef1 \u0111a d\u1ea1ng c\u1ee7a tr\u1ee5c h\u1ed1c-l\u00f4ng nhung. Khi so s\u00e1nh v\u1edbi c\u00e1c d\u00f2ng t\u1ebf b\u00e0o th\u00f4ng th\u01b0\u1eddng, c\u00e1c l\u1edbp \u0111\u01a1n ru\u1ed9t th\u1ec3 hi\u1ec7n t\u00ednh to\u00e0n v\u1eb9n c\u1ee7a h\u00e0ng r\u00e0o l\u1edbn h\u01a1n, bi\u1ec3u hi\u1ec7n m\u1ee9c \u0111\u1ed9 cao h\u01a1n c\u1ee7a c\u00e1c d\u1ea5u hi\u1ec7u bi\u1ec7t h\u00f3a quan tr\u1ecdng v\u00e0 c\u00f3 h\u00ecnh th\u00e1i \u0111\u1ea1i di\u1ec7n nhi\u1ec1u h\u01a1n cho ru\u1ed9t trong c\u01a1 th\u1ec3 s\u1ed1ng.<\/p>\n<p style=\"text-align: justify;\">C\u00e1c \u1ee9ng d\u1ee5ng c\u1ee7a nu\u00f4i c\u1ea5y organoid ru\u1ed9t bao g\u1ed3m nghi\u00ean c\u1ee9u s\u1ef1 ph\u00e1t tri\u1ec3n v\u00e0 ch\u1ee9c n\u0103ng c\u1ee7a bi\u1ec3u m\u00f4 ru\u1ed9t, m\u00f4 h\u00ecnh h\u00f3a c\u00e1c b\u1ec7nh \u0111\u01b0\u1eddng ru\u1ed9t, s\u00e0ng l\u1ecdc h\u1ee3p ch\u1ea5t v\u00e0 c\u00e1c ti\u1ebfp c\u1eadn li\u1ec7u ph\u00e1p t\u00e1i t\u1ea1o. C\u00e1c nu\u00f4i c\u1ea5y \u0111\u01a1n l\u1edbp ru\u1ed9t v\u00e0 ALI \u0111\u1eb7c bi\u1ec7t ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c x\u00e9t nghi\u1ec7m t\u00ednh th\u1ea5m v\u00e0 nghi\u00ean c\u1ee9u v\u1ec1 c\u00e1c b\u1ec7nh truy\u1ec1n nhi\u1ec5m do d\u1ec5 ti\u1ebfp c\u1eadn b\u1ec1 m\u1eb7t \u0111\u1ec9nh c\u1ee7a t\u1ebf b\u00e0o. B\u1ed9 kit n\u00e0y y\u00eau c\u1ea7u IntestiCult\u2122 Organoid Growth Medium (Ng\u01b0\u1eddi; Catalog #06010) \u0111\u1ec3 b\u1eaft \u0111\u1ea7u v\u00e0 m\u1edf r\u1ed9ng c\u00e1c organoid ru\u1ed9t tr\u01b0\u1edbc khi bi\u1ec7t h\u00f3a.<\/p>\n<h3 style=\"text-align: justify;\"><strong>T\u00ednh n\u0103ng n\u1ed5i b\u1eadt<\/strong><\/h3>\n<ul>\n<li>\n<p style=\"text-align: justify;\">Cho ph\u00e9p <strong>t\u1ea1o ra c\u00e1c m\u1eabu nu\u00f4i c\u1ea5y organoid ru\u1ed9t<\/strong> v\u1edbi t\u1ef7 l\u1ec7 sinh l\u00fd c\u1ee7a c\u00e1c qu\u1ea7n th\u1ec3 t\u1ebf b\u00e0o g\u1ed1c v\u00e0 t\u1ebf b\u00e0o bi\u1ec7t h\u00f3a<\/p>\n<\/li>\n<li>\n<p style=\"text-align: justify;\">Cho ph\u00e9p d\u1ec5 d\u00e0ng ti\u1ebfp c\u1eadn b\u1ec1 m\u1eb7t \u0111\u1ec9nh b\u1eb1ng c\u00e1ch chuy\u1ec3n \u0111\u1ed5i c\u00e1c c\u01a1 quan ru\u1ed9t th\u00e0nh c\u00e1c nu\u00f4i c\u1ea5y \u0111\u01a1n l\u1edbp v\u00e0 ALI<\/p>\n<\/li>\n<li>\n<p style=\"text-align: justify;\">T\u1ea1o ra <strong>k\u1ebft qu\u1ea3 nh\u1ea5t qu\u00e1n<\/strong> qua c\u00e1c l\u1ea7n c\u1ea5y chuy\u1ec1n v\u00e0 c\u00e1c m\u1eabu l\u1eb7p l\u1ea1i<\/p>\n<\/li>\n<\/ul>\n<h3 style=\"text-align: justify;\"><strong>\u1ee8ng d\u1ee5ng<\/strong><\/h3>\n<ul>\n<li>Nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o, Bi\u1ec7t h\u00f3a, M\u1edf r\u1ed9ng, Duy tr\u00ec, Nu\u00f4i c\u1ea5y organoid<\/li>\n<\/ul>\n<h3 style=\"text-align: justify;\"><strong>Th\u00e0nh ph\u1ea7n b\u1ed9 kit<\/strong><\/h3>\n<p style=\"text-align: justify;\">HepatiCult\u2122 Organoid Kit (Human) (Catalog #\u00a0100-0214) bao g\u1ed3m:<\/p>\n<ul>\n<li>\n<p style=\"text-align: justify;\">IntestiCult\u2122 ODM Human Basal Medium (Catalog # 100-0212), 50 mL<\/p>\n<\/li>\n<li>\n<p style=\"text-align: justify;\">Organoid Supplement (Catalog #100-0191), chai 50 mL<\/p>\n<\/li>\n<\/ul>\n<h3 style=\"text-align: justify;\"><strong>D\u1eef li\u1ec7u<\/strong><\/h3>\n<p style=\"text-align: center;\"><img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/uploads\/or7MdyW5xGq6zxCsvKk35ZAHJJF4qkRcFNkyl3RD.jpg\" alt=\"C\u00e1c c\u01a1 quan ru\u1ed9t ng\u01b0\u1eddi ph\u00e2n bi\u1ec7t th\u1ec3 hi\u1ec7n h\u00ecnh th\u00e1i n\u1ea3y ch\u1ed3i\" width=\"426\" height=\"206\" \/><\/p>\n<p style=\"text-align: center;\"><em>H\u00ecnh 1. C\u00e1c c\u01a1 quan ru\u1ed9t ng\u01b0\u1eddi bi\u1ec7t h\u00f3a th\u1ec3 hi\u1ec7n h\u00ecnh th\u00e1i n\u1ea3y ch\u1ed3i<\/em><\/p>\n<p style=\"text-align: justify;\">(A) C\u00e1c organoid \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y trong IntestiCult\u2122 OGM ch\u1ee7 y\u1ebfu l\u00e0 nang (cystic). (B) Khi chuy\u1ec3n sang IntestiCult\u2122 ODM, c\u00e1c organoid ph\u00e1t tri\u1ec3n bi\u1ec3u m\u00f4 d\u00e0y v\u1edbi h\u00ecnh th\u00e1i n\u1ea3y ch\u1ed3i r\u00f5 r\u1ec7t, bi\u1ec3u th\u1ecb tr\u1ea1ng th\u00e1i bi\u1ec7t h\u00f3a cao h\u01a1n. H\u00ecnh \u1ea3nh c\u00e1c organoid \u0111\u01b0\u1ee3c ch\u1ee5p v\u00e0o ng\u00e0y th\u1ee9 5 c\u1ee7a qu\u00e1 tr\u00ecnh m\u1edf r\u1ed9ng ho\u1eb7c bi\u1ec7t h\u00f3a t\u01b0\u01a1ng \u1ee9ng.<\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/uploads\/wX8FveNHp6uWMYKr6LTqQqNUbAEWpzumJRFj0NfO.jpg\" alt=\"C\u00e1c c\u01a1 quan ru\u1ed9t ch\u1ee9a t\u1ef7 l\u1ec7 cao h\u01a1n c\u00e1c lo\u1ea1i t\u1ebf b\u00e0o tr\u01b0\u1edfng th\u00e0nh sau khi bi\u1ec7t h\u00f3a trong IntestiCult\u2122 ODM\" width=\"408\" height=\"329\" \/><\/p>\n<p style=\"text-align: center;\"><em>H\u00ecnh 2. C\u00e1c organoid ru\u1ed9t ch\u1ee9a t\u1ef7 l\u1ec7 cao h\u01a1n c\u00e1c lo\u1ea1i t\u1ebf b\u00e0o tr\u01b0\u1edfng th\u00e0nh sau khi bi\u1ec7t h\u00f3a trong IntestiCult\u2122 ODM<\/em><\/p>\n<p style=\"text-align: justify;\">(A, B) C\u00e1c organoid nu\u00f4i c\u1ea5y trong IntestiCult\u2122 OGM \u0111\u01b0\u1ee3c l\u00e0m gi\u00e0u cho c\u00e1c t\u1ebf b\u00e0o t\u0103ng sinh Ki-67+ (A), trong khi ch\u1ee9a m\u1ed9t s\u1ed1 lo\u1ea1i t\u1ebf b\u00e0o bi\u1ec7t h\u00f3a nh\u01b0 t\u1ebf b\u00e0o h\u00ecnh \u0111\u00e0i (MUC2, A), t\u1ebf b\u00e0o ru\u1ed9t (KRT20, B) v\u00e0 t\u1ebf b\u00e0o n\u1ed9i ti\u1ebft ru\u1ed9t (CHGA, B). (C, D) Khi chuy\u1ec3n sang IntestiCult\u2122 ODM, c\u00e1c organoid ch\u1ee9a m\u1ed9t s\u1ed1 l\u01b0\u1ee3ng nh\u1ecf t\u1ebf b\u00e0o t\u0103ng sinh Ki-67+ (C, m\u0169i t\u00ean), v\u1edbi t\u1ef7 l\u1ec7 sinh l\u00fd nhi\u1ec1u h\u01a1n c\u1ee7a c\u00e1c t\u1ebf b\u00e0o h\u00ecnh \u0111\u00e0i (MUC2, C), t\u1ebf b\u00e0o ru\u1ed9t (KRT20, D) v\u00e0 t\u1ebf b\u00e0o n\u1ed9i ti\u1ebft ru\u1ed9t d\u01b0\u01a1ng t\u00ednh v\u1edbi chromogranin A- (CHGA-) (D, m\u0169i t\u00ean).<\/p>\n<figure class=\"image\"><img decoding=\"async\" style=\"aspect-ratio: 851\/554;\" src=\"https:\/\/biogroupvietnam.com\/uploads\/Flx1yg0l0m38d0C2y1PKtIvKoPmtsuzKGSaZRz3k.png\" width=\"851\" height=\"554\" \/><\/figure>\n<p style=\"text-align: center;\"><em>H\u00ecnh 3: Bi\u1ec7t h\u00f3a bi\u1ec3u m\u00f4 ru\u1ed9t \u1edf b\u1ec1 m\u1eb7t kh\u00ed-l\u1ecfng (ALI) b\u1eb1ng IntestiCult\u2122 ODM<\/em><\/p>\n<p style=\"text-align: justify;\">(A \u2013 E) Nu\u00f4i c\u1ea5y c\u00e1c l\u1edbp \u0111\u01a1n c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb organoids d\u01b0\u1edbi d\u1ea1ng nu\u00f4i c\u1ea5y ALI th\u00fac \u0111\u1ea9y s\u1ef1 bi\u1ec7t h\u00f3a h\u01a1n n\u1eefa c\u1ee7a c\u00e1c nu\u00f4i c\u1ea5y bi\u1ec3u m\u00f4 ru\u1ed9t nh\u01b0 \u0111\u01b0\u1ee3c th\u1ea5y b\u1eb1ng nh\u1eefng thay \u0111\u1ed5i trong bi\u1ec3u hi\u1ec7n gen \u0111\u01b0\u1ee3c \u0111o b\u1eb1ng RT-qPCR. \u0110\u1ecbnh l\u01b0\u1ee3ng t\u01b0\u01a1ng \u0111\u1ed1i (RQ) cho t\u1eebng d\u1ea5u hi\u1ec7u \u0111\u01b0\u1ee3c hi\u1ec3n th\u1ecb so v\u1edbi gen qu\u1ea3n l\u00fd actB v\u00e0 TBP v\u00e0 \u0111\u01b0\u1ee3c chu\u1ea9n h\u00f3a \u0111\u1ed1i v\u1edbi c\u00e1c c\u01a1 quan ch\u01b0a bi\u1ec7t h\u00f3a \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y trong IntestiCult OGM (Ng\u01b0\u1eddi). C\u00e1c d\u1ea5u hi\u1ec7u ti\u1ec1n th\u00e2n (A) Lgr5 v\u00e0 (B) Axin2 gi\u1ea3m \u0111\u00e1ng k\u1ec3 trong c\u1ea3 l\u1edbp \u0111\u01a1n ch\u00ecm v\u00e0 nu\u00f4i c\u1ea5y ALI, trong khi c\u00e1c d\u1ea5u hi\u1ec7u c\u1ee7a t\u1ebf b\u00e0o ru\u1ed9t (KRT20, C), t\u1ebf b\u00e0o h\u00ecnh \u0111\u00e0i (MUC2, D) v\u00e0 t\u1ebf b\u00e0o n\u1ed9i ti\u1ebft ru\u1ed9t (CHGA, E) t\u0103ng \u0111\u00e1ng k\u1ec3. Gi\u1ea3m th\u00eam Axin2 \u0111\u01b0\u1ee3c th\u1ea5y trong l\u1edbp \u0111\u01a1n ALI v\u1edbi s\u1ef1 gia t\u0103ng bi\u1ec3u hi\u1ec7n c\u1ee7a KRT20, MUC2 v\u00e0 CHGA. (F, G) So s\u00e1nh c\u00e1c m\u1eb7t c\u1eaft ngang c\u1ee7a l\u1edbp \u0111\u01a1n organoid \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y trong IntestiCult\u2122 ODM d\u01b0\u1edbi d\u1ea1ng (F) nu\u00f4i c\u1ea5y ch\u00ecm ho\u1eb7c (G) t\u1ea1i ALI cho th\u1ea5y s\u1ef1 bi\u1ec7t h\u00f3a th\u00eam c\u1ee7a bi\u1ec3u m\u00f4 ru\u1ed9t v\u1edbi t\u1ef7 l\u1ec7 t\u0103ng c\u00e1c t\u1ebf b\u00e0o h\u00ecnh \u0111\u00e0i v\u00e0 ch\u1ea5t nh\u1ea7y ngo\u1ea1i b\u00e0o (MUC2, m\u00e0u xanh l\u00e1 c\u00e2y).<\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.stemcell.com\/products\/intesticult-organoid-differentiation-medium-human.html\">Xem th\u00eam d\u1eef li\u1ec7u t\u1ea1i \u0111\u00e2y<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>T\u1ed5ng quan v\u1ec1 b\u1ed9 m\u00f4i tr\u01b0\u1eddng bi\u1ec7t h\u00f3a organoid ru\u1ed9t IntestiCult\u2122 B\u1ed9 M\u00f4i Tr\u01b0\u1eddng Bi\u1ec7t H\u00f3a Organoid Ru\u1ed9t IntestiCult\u2122 (Ng\u01b0\u1eddi) l\u00e0 m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y ho\u00e0n ch\u1ec9nh h\u1ed7 tr\u1ee3 bi\u1ec7t h\u00f3a th\u00eam c\u00e1c c\u01a1 quan ru\u1ed9t \u1edf ba chi\u1ec1u (3D) ho\u1eb7c \u1edf 2D d\u01b0\u1edbi d\u1ea1ng c\u00e1c l\u1edbp \u0111\u01a1n ho\u1eb7c nu\u00f4i c\u1ea5y b\u1ec1 m\u1eb7t kh\u00f4ng kh\u00ed-l\u1ecfng<\/p>\n<div class=\"klb-readmore post-buttons\"><a class=\"button rounded primary\" href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/bo-moi-truong-biet-hoa-organoid-ruot-intesticult-nguoi\/\">Read More <i 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