{"id":4318,"date":"2024-02-25T07:39:27","date_gmt":"2024-02-25T07:39:27","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/service\/how-to-use-extracellular-vesicle-sec-columns\/"},"modified":"2025-09-29T13:01:39","modified_gmt":"2025-09-29T06:01:39","slug":"huong-dan-su-dung-cot-sac-ky-loai-tru-kich-thuoc-sec","status":"publish","type":"service","link":"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/huong-dan-su-dung-cot-sac-ky-loai-tru-kich-thuoc-sec\/","title":{"rendered":"H\u01b0\u1edbng D\u1eabn S\u1eed D\u1ee5ng\u00a0C\u1ed9t S\u1eafc K\u00fd Lo\u1ea1i Tr\u1eeb K\u00edch Th\u01b0\u1edbc (SEC)"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_83 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/huong-dan-su-dung-cot-sac-ky-loai-tru-kich-thuoc-sec\/#Quy_trinh_phan_lap_tui_ngoai_bao_voi_cot_sac_ky_SEC\" >Quy tr\u00ecnh ph\u00e2n l\u1eadp t\u00fai ngo\u1ea1i b\u00e0o v\u1edbi c\u1ed9t s\u1eafc k\u00fd SEC<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/huong-dan-su-dung-cot-sac-ky-loai-tru-kich-thuoc-sec\/#Huong_dan_su_dung_cot_sac_ky_loai_tru_kich_thuoc_SEC\" >H\u01b0\u1edbng d\u1eabn s\u1eed d\u1ee5ng&nbsp;c\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC)<\/a><\/li><\/ul><\/nav><\/div>\n<h3><span class=\"ez-toc-section\" id=\"Quy_trinh_phan_lap_tui_ngoai_bao_voi_cot_sac_ky_SEC\"><\/span><strong>Quy tr\u00ecnh ph\u00e2n l\u1eadp t\u00fai ngo\u1ea1i b\u00e0o v\u1edbi c\u1ed9t s\u1eafc k\u00fd SEC<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<h3 style=\"text-align:center;\"><img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/MO4A8oeZhArguphNmig9RqGaewvOX4RaKm119dQC.png\" alt=\"\" width=\"754\" height=\"281\"><\/h3>\n<h3><span class=\"ez-toc-section\" id=\"Huong_dan_su_dung_cot_sac_ky_loai_tru_kich_thuoc_SEC\"><\/span><strong>H\u01b0\u1edbng d\u1eabn s\u1eed d\u1ee5ng&nbsp;<\/strong><a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/nghien-cuu-sinh-hoc\/phan-lap-te-bao\/extracellular-vesicle-sec-columns.html\"><strong>c\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC)<\/strong><\/a><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ol>\n<li>\u1ed4n \u0111\u1ecbnh c\u1ed9t SEC \u1edf v\u1ecb tr\u00ed th\u1eb3ng \u0111\u1ee9ng.<\/li>\n<li>Th\u00e1o n\u1eafp c\u1ed9t (n\u1eafp tr\u00ean v\u00e0 d\u01b0\u1edbi) v\u00e0 \u0111\u1ec3 dung d\u1ecbch \u0111\u1ec7m r\u00e3 \u0111\u00f4ng ch\u1ea3y qua c\u1ed9t. Lo\u1ea1i b\u1ecf ph\u1ea7n dung d\u1ecbch ch\u1ea3y ra. Kh\u00f4ng \u0111\u1ec3 c\u1ed9t b\u1ecb kh\u00f4.<\/li>\n<li>Cho t\u1ed5ng c\u1ed9ng 150mL D-PBS (Kh\u00f4ng c\u00f3 Ca++ v\u00e0 Mg++) v\u00e0o c\u1ed9t. Lo\u1ea1i b\u1ecf ph\u1ea7n dung d\u1ecbch ch\u1ea3y ra. Ngay l\u1eadp t\u1ee9c ti\u1ebfn h\u00e0nh b\u01b0\u1edbc 4.<\/li>\n<\/ol>\n<p>&nbsp; &nbsp; &nbsp; &nbsp;L\u01afU \u00dd: Gi\u1eef cho n\u1ec1n gel c\u1ed9t lu\u00f4n \u1ea9m.<\/p>\n<ol>\n<li>N\u1ea1p 5 &#8211; 20mL m\u1eabu v\u00e0o c\u1ed9t v\u00e0 b\u1eaft \u0111\u1ea7u thu c\u00e1c gi\u1ecdt dung d\u1ecbch ch\u1ea3y ra, m\u1ed7i gi\u1ecdt 1mL.<\/li>\n<li>Khi t\u1ea5t c\u1ea3 m\u1eabu \u0111\u00e3 \u0111\u01b0\u1ee3c n\u1ea1p v\u00e0o n\u1ec1n gel, ti\u1ebfp t\u1ee5c th\u00eam D-PBS v\u00e0o c\u1ed9t.<\/li>\n<li>Ti\u1ebfp t\u1ee5c thu th\u1eadp c\u00e1c gi\u1ecdt 1 mL cho \u0111\u1ebfn khi thu \u0111\u01b0\u1ee3c c\u00e1c ph\u1ea7n mong mu\u1ed1n.<\/li>\n<\/ol>\n<p>&nbsp;&nbsp; &nbsp; &nbsp; L\u01afU \u00dd: EV th\u01b0\u1eddng thu \u0111\u01b0\u1ee3c t\u1eeb gi\u1ecdt 15 &#8211; 35. Protein thu \u0111\u01b0\u1ee3c t\u1eeb gi\u1ecdt 36 tr\u1edf \u0111i. K\u1ebft qu\u1ea3 c\u00f3 th\u1ec3 kh\u00e1c nhau t\u00f9y theo lo\u1ea1i m\u1eabu. Gi\u1eef l\u1ea1i t\u1ea5t c\u1ea3 c\u00e1c th\u00e0nh ph\u1ea7n \u0111\u1ec3 ph\u00e2n t\u00edch.<\/p>\n<ol>\n<li>\u0110\u1ec3 r\u1eeda c\u1ed9t, \u0111\u1ed5 D-PBS l\u00ean tr\u00ean c\u1ed9t v\u00e0 cho 150 mL D-PBS ch\u1ea3y qua Lo\u1ea1i b\u1ecf ph\u1ea7n dung d\u1ecbch ch\u1ea3y ra.<\/li>\n<li>Th\u00eam 1 mL D-PBS v\u00e0o c\u1ed9t v\u00e0 \u0111\u00f3ng n\u1eafp tr\u00ean v\u00e0 d\u01b0\u1edbi c\u1ed9t. \u0110i\u1ec1u n\u00e0y s\u1ebd \u0111\u1ea3m b\u1ea3o r\u1eb1ng n\u1ec1n gel v\u1eabn \u01b0\u1edbt.<\/li>\n<\/ol>\n<p>&nbsp;&nbsp; &nbsp; &nbsp; L\u01afU \u00dd: B\u1ea3o qu\u1ea3n c\u1ed9t \u1edf nhi\u1ec7t \u0111\u1ed9 2 &#8211; 8\u00b0C; m\u1ed7i c\u1ed9t c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng t\u1ed1i \u0111a 5 l\u1ea7n. C\u1ed9t SEC kh\u00f4ng \u0111\u01b0\u1ee3c coi l\u00e0 v\u00f4 tr\u00f9ng.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Quy tr\u00ecnh ph\u00e2n l\u1eadp t\u00fai ngo\u1ea1i b\u00e0o v\u1edbi c\u1ed9t s\u1eafc k\u00fd SEC H\u01b0\u1edbng d\u1eabn s\u1eed d\u1ee5ng&nbsp;c\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC) \u1ed4n \u0111\u1ecbnh c\u1ed9t SEC \u1edf v\u1ecb tr\u00ed th\u1eb3ng \u0111\u1ee9ng. Th\u00e1o n\u1eafp c\u1ed9t (n\u1eafp tr\u00ean v\u00e0 d\u01b0\u1edbi) v\u00e0 \u0111\u1ec3 dung d\u1ecbch \u0111\u1ec7m r\u00e3 \u0111\u00f4ng ch\u1ea3y qua c\u1ed9t. Lo\u1ea1i b\u1ecf ph\u1ea7n dung d\u1ecbch ch\u1ea3y<\/p>\n<div class=\"klb-readmore post-buttons\"><a class=\"button rounded primary\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/huong-dan-su-dung-cot-sac-ky-loai-tru-kich-thuoc-sec\/\">Read More <i class=\"klbth-icon-right-arrow\"><\/i><\/a><\/div>\n","protected":false},"author":1,"featured_media":5782,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"service-category":[156],"class_list":["post-4318","service","type-service","status-publish","format-standard","has-post-thumbnail","hentry","service-category-quy-trinh-huong-dan-su-dung"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>H\u01b0\u1edbng D\u1eabn S\u1eed D\u1ee5ng\u00a0C\u1ed9t S\u1eafc K\u00fd Lo\u1ea1i Tr\u1eeb K\u00edch Th\u01b0\u1edbc (SEC)<\/title>\n<meta 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