{"id":4318,"date":"2024-02-25T07:39:27","date_gmt":"2024-02-25T07:39:27","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/service\/how-to-use-extracellular-vesicle-sec-columns\/"},"modified":"2025-09-29T13:01:39","modified_gmt":"2025-09-29T06:01:39","slug":"huong-dan-su-dung-cot-sac-ky-loai-tru-kich-thuoc-sec","status":"publish","type":"service","link":"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/huong-dan-su-dung-cot-sac-ky-loai-tru-kich-thuoc-sec\/","title":{"rendered":"H\u01b0\u1edbng D\u1eabn S\u1eed D\u1ee5ng\u00a0C\u1ed9t S\u1eafc K\u00fd Lo\u1ea1i Tr\u1eeb K\u00edch Th\u01b0\u1edbc (SEC)"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/huong-dan-su-dung-cot-sac-ky-loai-tru-kich-thuoc-sec\/#Quy_trinh_phan_lap_tui_ngoai_bao_voi_cot_sac_ky_SEC\" >Quy tr\u00ecnh ph\u00e2n l\u1eadp t\u00fai ngo\u1ea1i b\u00e0o v\u1edbi c\u1ed9t s\u1eafc k\u00fd SEC<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/huong-dan-su-dung-cot-sac-ky-loai-tru-kich-thuoc-sec\/#Huong_dan_su_dung_cot_sac_ky_loai_tru_kich_thuoc_SEC\" >H\u01b0\u1edbng d\u1eabn s\u1eed d\u1ee5ng&nbsp;c\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC)<\/a><\/li><\/ul><\/nav><\/div>\n<h3><span class=\"ez-toc-section\" id=\"Quy_trinh_phan_lap_tui_ngoai_bao_voi_cot_sac_ky_SEC\"><\/span><strong>Quy tr\u00ecnh ph\u00e2n l\u1eadp t\u00fai ngo\u1ea1i b\u00e0o v\u1edbi c\u1ed9t s\u1eafc k\u00fd SEC<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<h3 style=\"text-align:center;\"><img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/MO4A8oeZhArguphNmig9RqGaewvOX4RaKm119dQC.png\" alt=\"\" width=\"754\" height=\"281\"><\/h3>\n<h3><span class=\"ez-toc-section\" id=\"Huong_dan_su_dung_cot_sac_ky_loai_tru_kich_thuoc_SEC\"><\/span><strong>H\u01b0\u1edbng d\u1eabn s\u1eed d\u1ee5ng&nbsp;<\/strong><a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/nghien-cuu-sinh-hoc\/phan-lap-te-bao\/extracellular-vesicle-sec-columns.html\"><strong>c\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC)<\/strong><\/a><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ol>\n<li>\u1ed4n \u0111\u1ecbnh c\u1ed9t SEC \u1edf v\u1ecb tr\u00ed th\u1eb3ng \u0111\u1ee9ng.<\/li>\n<li>Th\u00e1o n\u1eafp c\u1ed9t (n\u1eafp tr\u00ean v\u00e0 d\u01b0\u1edbi) v\u00e0 \u0111\u1ec3 dung d\u1ecbch \u0111\u1ec7m r\u00e3 \u0111\u00f4ng ch\u1ea3y qua c\u1ed9t. Lo\u1ea1i b\u1ecf ph\u1ea7n dung d\u1ecbch ch\u1ea3y ra. Kh\u00f4ng \u0111\u1ec3 c\u1ed9t b\u1ecb kh\u00f4.<\/li>\n<li>Cho t\u1ed5ng c\u1ed9ng 150mL D-PBS (Kh\u00f4ng c\u00f3 Ca++ v\u00e0 Mg++) v\u00e0o c\u1ed9t. Lo\u1ea1i b\u1ecf ph\u1ea7n dung d\u1ecbch ch\u1ea3y ra. Ngay l\u1eadp t\u1ee9c ti\u1ebfn h\u00e0nh b\u01b0\u1edbc 4.<\/li>\n<\/ol>\n<p>&nbsp; &nbsp; &nbsp; &nbsp;L\u01afU \u00dd: Gi\u1eef cho n\u1ec1n gel c\u1ed9t lu\u00f4n \u1ea9m.<\/p>\n<ol>\n<li>N\u1ea1p 5 &#8211; 20mL m\u1eabu v\u00e0o c\u1ed9t v\u00e0 b\u1eaft \u0111\u1ea7u thu c\u00e1c gi\u1ecdt dung d\u1ecbch ch\u1ea3y ra, m\u1ed7i gi\u1ecdt 1mL.<\/li>\n<li>Khi t\u1ea5t c\u1ea3 m\u1eabu \u0111\u00e3 \u0111\u01b0\u1ee3c n\u1ea1p v\u00e0o n\u1ec1n gel, ti\u1ebfp t\u1ee5c th\u00eam D-PBS v\u00e0o c\u1ed9t.<\/li>\n<li>Ti\u1ebfp t\u1ee5c thu th\u1eadp c\u00e1c gi\u1ecdt 1 mL cho \u0111\u1ebfn khi thu \u0111\u01b0\u1ee3c c\u00e1c ph\u1ea7n mong mu\u1ed1n.<\/li>\n<\/ol>\n<p>&nbsp;&nbsp; &nbsp; &nbsp; L\u01afU \u00dd: EV th\u01b0\u1eddng thu \u0111\u01b0\u1ee3c t\u1eeb gi\u1ecdt 15 &#8211; 35. Protein thu \u0111\u01b0\u1ee3c t\u1eeb gi\u1ecdt 36 tr\u1edf \u0111i. K\u1ebft qu\u1ea3 c\u00f3 th\u1ec3 kh\u00e1c nhau t\u00f9y theo lo\u1ea1i m\u1eabu. Gi\u1eef l\u1ea1i t\u1ea5t c\u1ea3 c\u00e1c th\u00e0nh ph\u1ea7n \u0111\u1ec3 ph\u00e2n t\u00edch.<\/p>\n<ol>\n<li>\u0110\u1ec3 r\u1eeda c\u1ed9t, \u0111\u1ed5 D-PBS l\u00ean tr\u00ean c\u1ed9t v\u00e0 cho 150 mL D-PBS ch\u1ea3y qua Lo\u1ea1i b\u1ecf ph\u1ea7n dung d\u1ecbch ch\u1ea3y ra.<\/li>\n<li>Th\u00eam 1 mL D-PBS v\u00e0o c\u1ed9t v\u00e0 \u0111\u00f3ng n\u1eafp tr\u00ean v\u00e0 d\u01b0\u1edbi c\u1ed9t. \u0110i\u1ec1u n\u00e0y s\u1ebd \u0111\u1ea3m b\u1ea3o r\u1eb1ng n\u1ec1n gel v\u1eabn \u01b0\u1edbt.<\/li>\n<\/ol>\n<p>&nbsp;&nbsp; &nbsp; &nbsp; L\u01afU \u00dd: B\u1ea3o qu\u1ea3n c\u1ed9t \u1edf nhi\u1ec7t \u0111\u1ed9 2 &#8211; 8\u00b0C; m\u1ed7i c\u1ed9t c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng t\u1ed1i \u0111a 5 l\u1ea7n. C\u1ed9t SEC kh\u00f4ng \u0111\u01b0\u1ee3c coi l\u00e0 v\u00f4 tr\u00f9ng.<\/p>\n","protected":false},"featured_media":5782,"parent":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-4318","service","type-service","status-publish","has-post-thumbnail","hentry","service-category-quy-trinh-huong-dan-su-dung"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>H\u01b0\u1edbng D\u1eabn S\u1eed D\u1ee5ng\u00a0C\u1ed9t S\u1eafc K\u00fd Lo\u1ea1i Tr\u1eeb K\u00edch Th\u01b0\u1edbc (SEC)<\/title>\n<meta name=\"description\" content=\"H\u01b0\u1edbng d\u1eabn c\u00e1ch s\u1eed d\u1ee5ng c\u1ed9t s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC) c\u1ee7a h\u00e3ng STEMCELL Technologies.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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