{"id":4353,"date":"2024-02-25T08:12:47","date_gmt":"2024-02-25T08:12:47","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/service\/depletion-of-red-blood-cells-from-fresh-blood-samples\/"},"modified":"2025-09-29T13:01:49","modified_gmt":"2025-09-29T06:01:49","slug":"phuong-phap-lam-lang-te-bao-hong-cau-tu-cac-mau-mau-tuoi","status":"publish","type":"service","link":"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/phuong-phap-lam-lang-te-bao-hong-cau-tu-cac-mau-mau-tuoi\/","title":{"rendered":"Ph\u01b0\u01a1ng Ph\u00e1p L\u00e0m L\u1eafng T\u1ebf B\u00e0o H\u1ed3ng C\u1ea7u T\u1eeb C\u00e1c M\u1eabu M\u00e1u T\u01b0\u01a1i"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/phuong-phap-lam-lang-te-bao-hong-cau-tu-cac-mau-mau-tuoi\/#Lam_giam_luong_te_bao_hong_cau_la_gi\" >L\u00e0m gi\u1ea3m l\u01b0\u1ee3ng t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u l\u00e0 g\u00ec?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/phuong-phap-lam-lang-te-bao-hong-cau-tu-cac-mau-mau-tuoi\/#Quy_trinh_HetaSep%E2%84%A2_cho_cac_mau_mau_tuoi_the_tich_it\" >Quy tr\u00ecnh HetaSep\u2122 cho c\u00e1c m\u1eabu m\u00e1u t\u01b0\u01a1i th\u1ec3 t\u00edch \u00edt.<\/a><\/li><\/ul><\/nav><\/div>\n<h3><span class=\"ez-toc-section\" id=\"Lam_giam_luong_te_bao_hong_cau_la_gi\"><\/span><strong>L\u00e0m gi\u1ea3m l\u01b0\u1ee3ng t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u l\u00e0 g\u00ec?<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>L\u00e0m gi\u1ea3m l\u01b0\u1ee3ng t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u (RBC) l\u00e0 m\u1ed9t ph\u1ea7n quan tr\u1ecdng trong vi\u1ec7c chu\u1ea9n b\u1ecb m\u1eabu cho c\u00e1c x\u00e9t nghi\u1ec7m nu\u00f4i c\u1ea5y c\u1ee5m t\u1ebf b\u00e0o (CFU) c\u1ee7a m\u00e1u t\u01b0\u01a1i. Vi\u1ec7c lo\u1ea1i b\u1ecf c\u00e1c t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u s\u1ebd gi\u1ea3m n\u1ec1n trong c\u00e1c \u0111\u0129a nu\u00f4i c\u1ea5y (H\u00ecnh 1) v\u00e0 \u0111\u1ea3m b\u1ea3o r\u1eb1ng c\u00e1c c\u1ee5m t\u1ebf b\u00e0o t\u1ea1o m\u00e1u c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c \u0111\u1ebfm ch\u00ednh x\u00e1c, cho d\u00f9 s\u1eed d\u1ee5ng th\u1ee7 c\u00f4ng b\u1eb1ng k\u00ednh hi\u1ec3n vi \u0111\u1ea3o ng\u01b0\u1ee3c hay h\u1ec7 t\u1ef1 \u0111\u1ed9ng s\u1eed d\u1ee5ng <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/xet-nghiem-sinh-hoc\/xet-nghiem-cum-te-bao\/stemvision-for-cfu-assay.html\">STEMvision<\/a>\u2122 (H\u00ecnh 2). C\u00e1c t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u ph\u1ea3i \u0111\u01b0\u1ee3c l\u00e0m c\u1ea1n ki\u1ec7t t\u1eeb m\u1eabu m\u00e1u t\u01b0\u01a1i ch\u01b0a qua x\u1eed l\u00fd (m\u1eabu m\u00e1u cu\u1ed1ng r\u1ed1n ho\u1eb7c m\u00e1u ngo\u1ea1i vi), l\u1edbp \u201cbufft coat\u201d, c\u00e1c t\u1ebf b\u00e0o \u0111\u01a1n nh\u00e2n v\u00e0 m\u00e1u \u0111\u01b0\u1ee3c \u0111\u01b0\u1ee3c ph\u00e2n t\u00e1ch b\u1eb1ng c\u00e1ch ly t\u00e2m t\u1ef7 tr\u1ecdng (v\u00ed d\u1ee5: <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/nghien-cuu-sinh-hoc\/phan-lap-te-bao\/dung-dich-lymphoprep-ly-tam-ty-trong.html\">Lymphoprep\u2122<\/a>) ho\u1eb7c \u0111\u01b0\u1ee3c x\u1eed l\u00fd b\u1eb1ng thi\u1ebft b\u1ecb t\u00e1ch t\u1ebf b\u00e0o t\u1ef1 \u0111\u1ed9ng (v\u00ed d\u1ee5: <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/xu-ly-te-bao\/xu-ly-mau-cuong-ron-tu-dong\/he-thong-axp-ii.html\">h\u1ec7 th\u1ed1ng AXP<\/a> cho m\u00e1u cu\u1ed1ng r\u1ed1n)<\/p>\n<p>STEMCELL Technologies Inc. \u0111\u00e3 ph\u00e1t tri\u1ec3n m\u1ed9t quy tr\u00ecnh \u0111\u01a1n gi\u1ea3n s\u1eed d\u1ee5ng HetaSep\u2122 \u0111\u1ec3 lo\u1ea1i b\u1ecf c\u00e1c t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u t\u1eeb c\u00e1c m\u1eabu m\u00e1u t\u01b0\u01a1i \u0111\u1ec3 chu\u1ea9n b\u1ecb cho vi\u1ec7c nu\u00f4i c\u1ea5y CFU. <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/nuoi-cay-te-bao\/te-bao-goc-tao-mau-hsc\/hetasep.html\">HetaSep<\/a>\u2122 l\u00e0 m\u1ed9t ch\u1ea5t k\u1ebft t\u1ee5 h\u1ed3ng c\u1ea7u \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 nhanh ch\u00f3ng t\u00e1ch c\u00e1c t\u1ebf b\u00e0o c\u00f3 nh\u00e2n ra kh\u1ecfi c\u00e1c t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u. Hi\u1ec7u qu\u1ea3 c\u1ee7a n\u00f3 d\u1ef1a tr\u00ean nguy\u00ean t\u1eafc r\u1eb1ng c\u00e1c t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u k\u1ebft t\u1ee5 v\u00e0o nhau v\u00e0 l\u1eafng xu\u1ed1ng nhanh h\u01a1n nhi\u1ec1u so v\u1edbi c\u00e1c t\u1ebf b\u00e0o kh\u00e1c. Quy tr\u00ecnh HetaSep\u2122 ch\u1ec9 c\u1ea7n 50 \u03bcL m\u00e1u, m\u1ea5t kho\u1ea3ng 15 &#8211; 20 ph\u00fat v\u00e0 ch\u1ec9 y\u00eau c\u1ea7u \u0111\u1ebfm t\u1ebf b\u00e0o khi b\u1eaft \u0111\u1ea7u quy tr\u00ecnh (l\u00fac t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c c\u1ea5y trong \u0111\u0129a CFU). Kh\u1ea3 n\u0103ng ph\u1ee5c h\u1ed3i c\u1ee7a c\u00e1c c\u1ee5m t\u1ebf b\u00e0o trong ph\u1ea7n m\u1eabu m\u00e1u \u0111\u01b0\u1ee3c x\u1eed l\u00fd HetaSep\u2122 \u0111\u1ec3 l\u00e0m suy gi\u1ea3m h\u1ed3ng c\u1ea7u l\u00e0 g\u1ea7n 100% n\u00ean ng\u01b0\u1eddi d\u00f9ng c\u00f3 th\u1ec3 tin t\u01b0\u1edfng r\u1eb1ng quy tr\u00ecnh HetaSep\u2122 kh\u00f4ng \u1ea3nh h\u01b0\u1edfng x\u1ea5u \u0111\u1ebfn s\u1ed1 l\u01b0\u1ee3ng ho\u1eb7c kh\u1ea3 n\u0103ng t\u1ed3n t\u1ea1i c\u1ee7a c\u00e1c t\u1ebf b\u00e0o ti\u1ec1n th\u00e2n t\u1ea1o m\u00e1u (H\u00ecnh 3). C\u00e1c t\u00ednh n\u0103ng n\u00e0y gi\u00fap d\u1ec5 d\u00e0ng k\u1ebft h\u1ee3p HetaSep\u2122 v\u00e0o quy tr\u00ecnh nu\u00f4i c\u1ea5y CFU c\u1ee7a c\u00e1c t\u1ed5 ch\u1ee9c.<\/p>\n<p style=\"text-align:center;\"><img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/tOkSHHtPrEeIc8eC9LWeqT8MQYvHlvjGtB9Hwpx5.png\" alt=\"\" width=\"331\" height=\"430\"><\/p>\n<p><i>H\u00ecnh 1. H\u00ecnh \u1ea3nh STEMvision\u2122 c\u1ee7a c\u00e1c th\u1eed nghi\u1ec7m CFU \u0111\u01b0\u1ee3c lo\u1ea1i b\u1ecf h\u1ed3ng c\u1ea7u tr\u01b0\u1edbc b\u1eb1ng HetaSep\u2122. (A) S\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u t\u1ed1i thi\u1ec3u cho nu\u00f4i c\u1ea5y CFU v\u1edbi x\u1eed l\u00fd HetaSep\u2122 tr\u01b0\u1edbc \u0111\u00f3 v\u00e0 (B) S\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u kh\u00f4ng th\u1ec3 ch\u1ea5p nh\u1eadn cho nu\u00f4i c\u1ea5y CFU m\u00e0 kh\u00f4ng x\u1eed l\u00fd HetaSep\u2122 tr\u01b0\u1edbc \u0111\u00f3. L\u01b0u \u00fd r\u1eb1ng c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y \u00edt c\u1ee5m t\u1ebf b\u00e0o h\u01a1n do n\u1ec1n h\u1ed3ng c\u1ea7u t\u0103ng l\u00ean.<\/i><\/p>\n<p style=\"text-align:center;\"><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/JNXPn0D0AQbqnc52SX6PlpUwLYQwAD5wrFdlHuY5.png\" alt=\"\" width=\"356\" height=\"528\"><\/p>\n<p><i>H\u00ecnh 2. C\u1ea7n lo\u1ea1i b\u1ecf h\u1ed3ng c\u1ea7u \u0111\u1ec3 \u0111\u1ebfm ch\u00ednh x\u00e1c c\u00e1c x\u00e9t nghi\u1ec7m nu\u1ed1i c\u1ea5y CFU v\u1edbi STEMvision\u2122<\/i><\/p>\n<p>\u0110\u01b0\u1ee3c hi\u1ec3n th\u1ecb \u0111\u00e2y l\u00e0 s\u1ed1 l\u01b0\u1ee3ng c\u1ee5m t\u1ebf b\u00e0o c\u00f3 trong x\u00e9t nghi\u1ec7m nu\u00f4i c\u1ea5y CFU m\u00e1u cu\u1ed1ng r\u1ed1n (A) kh\u00f4ng c\u00f3 v\u00e0 (B) c\u00f3 l\u00e0m gi\u1ea3m h\u1ed3ng c\u1ea7u b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng HetaSep\u2122 tr\u01b0\u1edbc khi c\u1ea5y (n = 10 m\u1eabu m\u00e1u cu\u1ed1ng r\u1ed1n t\u01b0\u01a1i). S\u1ef1 kh\u00e1c bi\u1ec7t l\u1edbn trong (A) l\u00e0 do t\u1ef7 l\u1ec7 cao c\u00e1c c\u1ee5m t\u1ebf b\u00e0o d\u01b0\u01a1ng t\u00ednh gi\u1ea3 \u0111\u01b0\u1ee3c STEMvision\u2122 \u0111\u1ebfm \u0111\u01b0\u1ee3c trong c\u00e1c m\u1eabu c\u00f3 n\u1ec1n h\u1ed3ng c\u1ea7u cao.<\/p>\n<p>Kh\u1ea3 n\u0103ng ph\u1ee5c h\u1ed3i c\u1ee7a CFU trong ph\u1ea7n \u0111\u01b0\u1ee3c x\u1eed l\u00fd gi\u1ea3m h\u1ed3ng c\u1ea7u l\u00e0 g\u1ea7n 100% n\u00ean ng\u01b0\u1eddi d\u00f9ng c\u00f3 th\u1ec3 tin t\u01b0\u1edfng r\u1eb1ng quy tr\u00ecnh HetaSepTM kh\u00f4ng \u1ea3nh h\u01b0\u1edfng x\u1ea5u \u0111\u1ebfn s\u1ed1 l\u01b0\u1ee3ng ho\u1eb7c kh\u1ea3 n\u0103ng s\u1ed1ng s\u00f3t c\u1ee7a c\u00e1c t\u1ebf b\u00e0o ti\u1ec1n th\u00e2n t\u1ea1o m\u00e1u (H\u00ecnh 3).<\/p>\n<p style=\"text-align:center;\"><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/9mISj8tuSfadL2tD5dasnE7RZ8Twh3QrwR8eQTRu.png\" alt=\"\" width=\"424\" height=\"263\"><\/p>\n<p><i>H\u00ecnh 3. Trung b\u00ecnh 97% s\u1ed1 c\u1ee5m t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c ph\u1ee5c h\u1ed3i sau khi l\u00e0m gi\u1ea3m l\u01b0\u1ee3ng t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u v\u1edbi HetaSep\u2122<\/i><\/p>\n<p>M\u1eabu m\u00e1u cu\u1ed1ng r\u1ed1n (n = 10) \u0111\u01b0\u1ee3c chia th\u00e0nh hai ph\u1ea7n, m\u1ed9t trong s\u1ed1 \u0111\u00f3 \u0111\u01b0\u1ee3c c\u1ea5y trong x\u00e9t nghi\u1ec7m nu\u00f4i c\u1ea5y CFU m\u00e0 kh\u00f4ng l\u00e0m suy gi\u1ea3m h\u1ed3ng c\u1ea7u v\u00e0 ph\u1ea7n c\u00f2n l\u1ea1i c\u00f3 l\u01b0\u1ee3ng h\u1ed3ng c\u1ea7u b\u1ecb c\u1ea1n ki\u1ec7t b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng quy tr\u00ecnh HetaSep\u2122 tr\u01b0\u1edbc khi c\u1ea5y. M\u1ed7i lo\u1ea1i m\u1eabu \u0111\u01b0\u1ee3c c\u1ea5y th\u00e0nh hai b\u1ea3n. C\u00e1c x\u00e9t nghi\u1ec7m nu\u00f4i c\u1ea5y CFU \u0111\u01b0\u1ee3c t\u00ednh theo c\u00e1ch th\u1ee7 c\u00f4ng v\u00e0 t\u1ef7 l\u1ec7 ph\u1ea7n tr\u0103m s\u1ed1 c\u1ee5m t\u1ebf b\u00e0o thu h\u1ed3i trong m\u1ed7i ph\u1ea7n b\u1ecb suy gi\u1ea3m h\u1ed3ng c\u1ea7u \u0111\u01b0\u1ee3c t\u00ednh to\u00e1n so v\u1edbi k\u1ebft qu\u1ea3 x\u00e9t nghi\u1ec7m nu\u00f4i c\u1ea5y CFU c\u1ee7a c\u00e1c t\u1ebf b\u00e0o kh\u00f4ng b\u1ecb c\u1ea1n ki\u1ec7t t\u1eeb c\u00f9ng m\u1ed9t m\u1eabu.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Quy_trinh_HetaSep%E2%84%A2_cho_cac_mau_mau_tuoi_the_tich_it\"><\/span><strong>Quy tr\u00ecnh HetaSep\u2122 cho c\u00e1c m\u1eabu m\u00e1u t\u01b0\u01a1i th\u1ec3 t\u00edch \u00edt.<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ol>\n<li>X\u00e1c \u0111\u1ecbnh n\u1ed3ng \u0111\u1ed9 t\u1ebf b\u00e0o c\u1ee7a m\u00e1u t\u01b0\u01a1i ban \u0111\u1ea7u<\/li>\n<li>Th\u00eam c\u00e1c ch\u1ea5t sau v\u00e0o \u1ed1ng 0,5 mL: 150 \u03bcL <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/nuoi-cay-te-bao\/te-bao-goc-trung-mo\/dung-dich-pbs.html\">Dulbecco\u2019s Phosphate Buffered Saline<\/a> (D-PBS) v\u1edbi 2% fetal bovine serum (FBS) (Danh m\u1ee5c # 07905). 50 \u03bcL m\u1eabu m\u00e1u t\u01b0\u01a1i. 40 \u03bcL HetaSep\u2122<\/li>\n<li>H\u1ec7 s\u1ed1 pha lo\u00e3ng m\u1eabu l\u00e0 4.8 (t\u1ed5ng th\u1ec3 t\u00edch 240 \u03bcL \u00f7 50 \u03bcL th\u1ec3 t\u00edch m\u1eabu).<\/li>\n<li>Tr\u1ed9n k\u1ef9 b\u1eb1ng pipet. Tr\u00e1nh t\u1ea1o bong b\u00f3ng. Kh\u00f4ng tr\u1ed9n xo\u00e1y.<\/li>\n<li>\u1ee6 15 &#8211; 20 ph\u00fat trong t\u1ee7 \u1ea5m 37\u00b0C. T\u1ed1t nh\u1ea5t n\u00ean th\u1ef1c hi\u1ec7n b\u01b0\u1edbc n\u00e0y b\u1eb1ng c\u00e1ch \u0111\u1eb7t c\u00e1c \u1ed1ng v\u00e0o t\u1ee7 \u1ea5m nu\u00f4i c\u1ea5y thay v\u00ec \u0111\u1eb7t trong n\u1ed3i c\u00e1ch th\u1ee7y, v\u00ec b\u01b0\u1edbc n\u00e0y d\u1eabn \u0111\u1ebfn nguy c\u01a1 nhi\u1ec5m b\u1ea9n b\u1edfi n\u01b0\u1edbc d\u01b0 khi m\u1edf \u1ed1ng. <i>L\u01b0u \u00fd: \u0110\u1ed9 ph\u1ee5c h\u1ed3i t\u1ebf b\u00e0o c\u00f3 th\u1ec3 gi\u1ea3m n\u1ebfu \u1ee7 m\u1eabu h\u01a1n 20 ph\u00fat. N\u1ebfu \u0111\u00e3 h\u01a1n 20 ph\u00fat, quay l\u1ea1i b\u01b0\u1edbc 3 ho\u1eb7c b\u1eaft \u0111\u1ea7u l\u1ea1i quy tr\u00ecnh v\u1edbi m\u1eabu m\u1edbi \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o ph\u1ee5c h\u1ed3i t\u1ed1i \u01b0u.<\/i><\/li>\n<li>Lo\u1ea1i b\u1ecf ph\u1ea7n d\u1ecbch n\u1ed5i \u1edf tr\u00ean b\u1eb1ng <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/nghien-cuu-sinh-hoc\/thiet-bi-co-ban\/micropipette-dien-tu.html\">micropipette<\/a> v\u00e0 chuy\u1ec3n sang \u1ed1ng m\u1edbi. H\u00fat t\u1eeb t\u1eeb ph\u1ea7n tr\u00ean, \u0111\u1ed3ng th\u1eddi di chuy\u1ec3n \u0111\u1ea7u h\u00fat xu\u1ed1ng d\u01b0\u1edbi. Kh\u00f4ng l\u00e0m x\u00e1o tr\u1ed9n h\u1ed3ng c\u1ea7u (H\u00ecnh 4B). L\u01b0u \u00fd: K\u00edch th\u01b0\u1edbc c\u1ee7a c\u00e1c t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u k\u1ebft t\u1ee5 c\u00f3 th\u1ec3 kh\u00e1c nhau do s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 hematocrit gi\u1eefa c\u00e1c lo\u1ea1i m\u1eabu. N\u1ebfu ph\u1ea7n h\u1ed3ng c\u1ea7u k\u1ebft t\u1ee5 b\u1ecb x\u00e1o tr\u1ed9n b\u1ea5t k\u1ef3 l\u00fac n\u00e0o, h\u00e3y quay l\u1ea1i b\u01b0\u1edbc 3.<\/li>\n<li>T\u00ednh n\u1ed3ng \u0111\u1ed9 t\u1ebf b\u00e0o c\u1ee7a m\u1eabu \u0111\u00e3 lo\u1ea1i b\u1ecf h\u1ed3ng c\u1ea7u b\u1eb1ng c\u00e1ch hi\u1ec7u ch\u1ec9nh h\u1ec7 s\u1ed1 pha lo\u00e3ng l\u00e0 4,8 (xem b\u01b0\u1edbc 2). V\u00ed d\u1ee5, n\u1ebfu n\u1ed3ng \u0111\u1ed9 t\u1ebf b\u00e0o ban \u0111\u1ea7u l\u00e0 5 x 106 t\u1ebf b\u00e0o\/mL, th\u00ec n\u1ed3ng \u0111\u1ed9 c\u1ee7a m\u1eabu \u0111\u00e3 lo\u1ea1i b\u1ecf h\u1ed3ng c\u1ea7u s\u1ebd l\u00e0 5 x 106 t\u1ebf b\u00e0o\/mL \u00f7 4,8 = 1,04 x 106 t\u1ebf b\u00e0o\/mL.<\/li>\n<li>Thi\u1ebft l\u1eadp nu\u00f4i c\u1ea5y CFU.<\/li>\n<\/ol>\n<p style=\"text-align:center;\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/ePbBwoiMUsYkIW0FSRuxd9qKEWuqvjdVmIZbunkQ.png\" alt=\"\" width=\"434\" height=\"212\"><\/p>\n<p><i>H\u00ecnh 4. H\u00ecnh th\u1ee9c m\u1eabu tr\u01b0\u1edbc v\u00e0 sau khi \u1ee7. \u1ed0ng 1,5 mL ch\u1ee9a m\u00e1u cu\u1ed1ng r\u1ed1n (A) ngay sau khi th\u00eam HetaSep\u2122 v\u00e0 (B) sau khi \u1ee7 20 ph\u00fat khi c\u00e1c t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u \u0111\u00e3 k\u1ebft t\u1ee5 \u1edf \u0111\u00e1y \u1ed1ng.<\/i><\/p>\n","protected":false},"featured_media":5825,"parent":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-4353","service","type-service","status-publish","has-post-thumbnail","hentry","service-category-quy-trinh-huong-dan-su-dung"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Ph\u01b0\u01a1ng Ph\u00e1p L\u00e0m L\u1eafng T\u1ebf B\u00e0o H\u1ed3ng C\u1ea7u T\u1eeb C\u00e1c M\u1eabu M\u00e1u T\u01b0\u01a1i<\/title>\n<meta name=\"description\" content=\"L\u00e0m gi\u1ea3m l\u01b0\u1ee3ng t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u (RBC) l\u00e0 m\u1ed9t 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