{"id":4364,"date":"2024-02-25T08:21:34","date_gmt":"2024-02-25T08:21:34","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/service\/mycoplasma-testing-for-cell-product-release\/"},"modified":"2025-09-29T13:01:50","modified_gmt":"2025-09-29T06:01:50","slug":"xet-nghiem-mycoplasma-trong-san-xuat-te-bao","status":"publish","type":"service","link":"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/","title":{"rendered":"X\u00e9t nghi\u1ec7m Mycoplasma Trong S\u1ea3n Xu\u1ea5t T\u1ebf B\u00e0o"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#FDA_My_da_dong_y_xet_nghiem_mycoplasma_bang_phuong_phap_khuech_dai_axit_nucleic_phuong_phap_NAT_cung_duoc_chap_nhan_boi_duoc_dien_My_Chau_Au_va_Nhat_Ban\" >FDA M\u1ef9 \u0111\u00e3 \u0111\u1ed3ng \u00fd x\u00e9t nghi\u1ec7m mycoplasma b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p khu\u1ebfch \u0111\u1ea1i axit nucleic (ph\u01b0\u01a1ng ph\u00e1p NAT), c\u0169ng \u0111\u01b0\u1ee3c ch\u1ea5p nh\u1eadn b\u1edfi d\u01b0\u1ee3c \u0111i\u1ec3n M\u1ef9, Ch\u00e2u \u00c2u v\u00e0 Nh\u1eadt B\u1ea3n.<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Tai_sao_xet_nghiem_mycoplasma_la_can_thiet\" >T\u1ea1i sao x\u00e9t nghi\u1ec7m mycoplasma l\u00e0 c\u1ea7n thi\u1ebft?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Mycoplasma_la_gi\" >Mycoplasma l\u00e0 g\u00ec?<\/a><ul class='ez-toc-list-level-4' ><li class='ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Dac_trung_cua_mycoplasma\" >\u0110\u1eb7c tr\u01b0ng c\u1ee7a mycoplasma<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#7_loai_chung_mycoplasma_duoc_phat_hien_theo_Duoc_dien_Nhat_Ban_Phien_ban_thu_17\" >7 lo\u1ea1i ch\u1ee7ng mycoplasma \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n theo D\u01b0\u1ee3c \u0111i\u1ec3n Nh\u1eadt B\u1ea3n Phi\u00ean b\u1ea3n th\u1ee9 17<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Phuong_phap_A_Phuong_phap_nuoi_cay_xet_nghiem_mycoplasma\" >Ph\u01b0\u01a1ng ph\u00e1p A: Ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y x\u00e9t nghi\u1ec7m mycoplasma<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Phuong_phap_B_Phuong_phap_nhuom_huynh_quang_DNA\" >Ph\u01b0\u01a1ng ph\u00e1p B: Ph\u01b0\u01a1ng ph\u00e1p nhu\u1ed9m hu\u1ef3nh quang DNA<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Phuong_phap_C_Ky_thuat_khuech_dai_axit_nucleic_phuong_phap_NAT\" >Ph\u01b0\u01a1ng ph\u00e1p C: K\u1ef9 thu\u1eadt khu\u1ebfch \u0111\u1ea1i axit nucleic (ph\u01b0\u01a1ng ph\u00e1p NAT)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Bo_kit_MycoFinder_phat_hien_gen_Mycoplasma\" >B\u1ed9 kit MycoFinder ph\u00e1t hi\u1ec7n gen Mycoplasma<\/a><ul class='ez-toc-list-level-4' ><li class='ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Cach_su_dung_bo_kit_phat_hien_gen_Mycoplasma_chuan_bi_thuoc_thu\" >C\u00e1ch s\u1eed d\u1ee5ng b\u1ed9 kit ph\u00e1t hi\u1ec7n gen Mycoplasma (chu\u1ea9n b\u1ecb thu\u1ed1c th\u1eed)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Phuong_phap_su_dung_qua_4_buoc_don_gian_de_thao_tac\" >Ph\u01b0\u01a1ng ph\u00e1p s\u1eed d\u1ee5ng qua 4 b\u01b0\u1edbc (\u0111\u01a1n gi\u1ea3n, d\u1ec5 thao t\u00e1c):<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-12\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Danh_gia_hieu_suat_cua_bo_kit_phat_hien_gen_Mycoplasma_va_tham_dinh_tuan_thu_duoc_dien_EP_JP\" >\u0110\u00e1nh gi\u00e1 hi\u1ec7u su\u1ea5t c\u1ee7a b\u1ed9 kit ph\u00e1t hi\u1ec7n gen Mycoplasma v\u00e0 th\u1ea9m \u0111\u1ecbnh tu\u00e2n th\u1ee7 d\u01b0\u1ee3c \u0111i\u1ec3n EP, JP<\/a><ul class='ez-toc-list-level-4' ><li class='ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-13\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Tai_sao_tham_dinh_la_can_thiet\" >T\u1ea1i sao th\u1ea9m \u0111\u1ecbnh l\u00e0 c\u1ea7n thi\u1ebft?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-14\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Huong_dan_tham_dinh_phuong_phap_NAT_trong_phat_hien_Mycoplasma_theo_duoc_dien_EP\" >H\u01b0\u1edbng d\u1eabn th\u1ea9m \u0111\u1ecbnh ph\u01b0\u01a1ng ph\u00e1p NAT trong ph\u00e1t hi\u1ec7n Mycoplasma theo d\u01b0\u1ee3c \u0111i\u1ec3n EP:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-15\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Nghien_cuu_ve_kha_nang_so_sanh_doi_voi_cac_phuong_phap_khac\" >Nghi\u00ean c\u1ee9u v\u1ec1 kh\u1ea3 n\u0103ng so s\u00e1nh \u0111\u1ed1i v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p kh\u00e1c:<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-16\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/xet-nghiem-mycoplasma-trong-san-xuat-te-bao\/#Tai_lieu_tham_khao\" >T\u00e0i li\u1ec7u tham kh\u1ea3o<\/a><\/li><\/ul><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"FDA_My_da_dong_y_xet_nghiem_mycoplasma_bang_phuong_phap_khuech_dai_axit_nucleic_phuong_phap_NAT_cung_duoc_chap_nhan_boi_duoc_dien_My_Chau_Au_va_Nhat_Ban\"><\/span>FDA M\u1ef9 \u0111\u00e3 \u0111\u1ed3ng \u00fd x\u00e9t nghi\u1ec7m mycoplasma b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p khu\u1ebfch \u0111\u1ea1i axit nucleic (ph\u01b0\u01a1ng ph\u00e1p NAT), c\u0169ng \u0111\u01b0\u1ee3c ch\u1ea5p nh\u1eadn b\u1edfi d\u01b0\u1ee3c \u0111i\u1ec3n M\u1ef9, Ch\u00e2u \u00c2u v\u00e0 Nh\u1eadt B\u1ea3n.<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<h3><span class=\"ez-toc-section\" id=\"Tai_sao_xet_nghiem_mycoplasma_la_can_thiet\"><\/span><strong>T\u1ea1i sao x\u00e9t nghi\u1ec7m mycoplasma l\u00e0 c\u1ea7n thi\u1ebft?<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>Do nh\u1eefng thay \u0111\u1ed5i trong lu\u1eadt (\u0110\u1ea1o lu\u1eadt v\u1ec1 \u0111\u1ea3m b\u1ea3o ch\u1ea5t l\u01b0\u1ee3ng, hi\u1ec7u qu\u1ea3 v\u00e0 an to\u00e0n c\u1ee7a s\u1ea3n ph\u1ea9m bao g\u1ed3m d\u01b0\u1ee3c ph\u1ea9m v\u00e0 thi\u1ebft b\u1ecb y t\u1ebf, \u0110\u1ea1o lu\u1eadt v\u1ec1 an to\u00e0n c\u1ee7a s\u1ea3n ph\u1ea9m thu\u1ed1c t\u00e1i t\u1ea1o, v.v.) v\u00e0o n\u0103m 2012, \u1ee9ng d\u1ee5ng th\u01b0\u01a1ng m\u1ea1i c\u1ee7a c\u00e1c s\u1ea3n ph\u1ea9m thu\u1ed1c t\u00e1i t\u1ea1o hi\u1ec7n \u0111\u00e3 \u0111\u01b0\u1ee3c cho ph\u00e9p. C\u00f9ng v\u1edbi \u0111\u00f3, x\u00e9t nghi\u1ec7m mycoplasma \u0111\u00e3 tr\u1edf th\u00e0nh b\u1eaft bu\u1ed9c nh\u01b0 m\u1ed9t ph\u1ea7n c\u1ee7a c\u00e1c x\u00e9t nghi\u1ec7m \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o an to\u00e0n cho c\u00e1c s\u1ea3n ph\u1ea9m thu\u1ed1c t\u00e1i t\u1ea1o.<\/p>\n<ul>\n<li>Kh\u00f3 \u0111\u1ea3m b\u1ea3o \u0111\u1ee7 s\u1ed1 l\u01b0\u1ee3ng s\u1ea3n ph\u1ea9m cu\u1ed1i c\u00f9ng c\u1ee7a y h\u1ecdc t\u00e1i t\u1ea1o l\u00e0m m\u1eabu th\u1eed v\u00ec s\u1ed1 l\u01b0\u1ee3ng s\u1ea3n ph\u1ea9m cu\u1ed1i c\u00f9ng c\u00f3 h\u1ea1n.<\/li>\n<li>Do s\u1ea3n ph\u1ea9m thu\u1ed1c t\u00e1i t\u1ea1o sau khi s\u1ea3n xu\u1ea5t c\u1ea7n s\u1eed d\u1ee5ng ngay n\u00ean c\u1ea7n c\u00f3 ph\u01b0\u01a1ng ph\u00e1p test nhanh.<\/li>\n<\/ul>\n<p>D\u01b0\u1ee3c \u0111i\u1ec3n M\u1ef9, Ch\u00e2u \u00c2u v\u00e0 Nh\u1eadt B\u1ea3n: K\u1ef9 thu\u1eadt khu\u1ebfch \u0111\u1ea1i axit nucleic \u0111\u00e3 \u0111\u01b0\u1ee3c b\u1ed5 sung nh\u01b0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p x\u00e9t nghi\u1ec7m nhanh mycoplasma.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Mycoplasma_la_gi\"><\/span><strong>Mycoplasma l\u00e0 g\u00ec?<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>Mycoplasma l\u00e0 vi khu\u1ea9n kh\u00f4ng v\u00e1ch t\u1ebf b\u00e0o, k\u00edch th\u01b0\u1edbc nh\u1ecf (0,2- 0,8\u00b5m), c\u00f3 h\u00ecnh d\u00e1ng \u0111a d\u1ea1ng.<\/p>\n<p>Mycoplasma, Ureaplasma, Spiroplasma, Acholeplasma v\u00e0 c\u00e1c vi khu\u1ea9n kh\u00e1c thu\u1ed9c \u0110\u1ed9ng v\u1eadt th\u00e2n m\u1ec1m (Mollicutes).<\/p>\n<h4><span class=\"ez-toc-section\" id=\"Dac_trung_cua_mycoplasma\"><\/span><strong>\u0110\u1eb7c tr\u01b0ng c\u1ee7a mycoplasma<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h4>\n<ul>\n<li>Ph\u00e2n b\u1ed1 r\u1ed9ng r\u00e3i trong gi\u1edbi \u0111\u1ed9ng v\u1eadt v\u00e0 th\u1ef1c v\u1eadt v\u00e0 s\u1ef1 l\u00e2y nhi\u1ec5m cho th\u1ea5y t\u00ednh \u0111\u1eb7c hi\u1ec7u c\u1ee7a lo\u00e0i.<\/li>\n<li>L\u00e2y nhi\u1ec5m cho ng\u01b0\u1eddi th\u00f4ng qua c\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u th\u1ef1c hi\u1ec7n nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o ho\u1eb7c huy\u1ebft thanh d\u00f9ng \u0111\u1ec3 nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o, v.v.<\/li>\n<li>C\u00f3 kh\u1ea3 n\u0103ng kh\u00e1ng thu\u1ed1c kh\u00e1ng sinh.<\/li>\n<li>L\u1ecdt c\u00e1c l\u1ecdc kh\u1eed tr\u00f9ng (0,22\u03bcm) v\u00ec k\u00edch th\u01b0\u1edbc nh\u1ecf.<\/li>\n<li>C\u00e1c t\u1ebf b\u00e0o b\u1ecb nhi\u1ec5m b\u1ec7nh kh\u00f4ng th\u1ec3 hi\u1ec7n t\u00e1c d\u1ee5ng g\u00e2y \u0111\u1ed9c t\u1ebf b\u00e0o.<\/li>\n<\/ul>\n<p>V\u1edbi nh\u1eefng \u0111\u1eb7c tr\u01b0ng n\u00e0y, kh\u00f3 ph\u00e1t hi\u1ec7n nhi\u1ec5m mycoplasma.<\/p>\n<p>C\u00f3 nhi\u1ec1u b\u00e1o c\u00e1o r\u1eb1ng c\u00e1c t\u1ebf b\u00e0o nu\u00f4i c\u1ea5y trong c\u00e1c ph\u00f2ng th\u00ed nghi\u1ec7m th\u01b0\u1eddng b\u1ecb nhi\u1ec5m mycoplasma. Vi\u1ec7c ph\u00e1t tri\u1ec3n c\u00e1c s\u1ea3n ph\u1ea9m y t\u1ebf s\u1eed d\u1ee5ng t\u1ebf b\u00e0o b\u1ecb \u00f4 nhi\u1ec5m s\u1ebd d\u1eabn \u0111\u1ebfn h\u1eadu qu\u1ea3 nghi\u00eam tr\u1ecdng.<\/p>\n<p>Do v\u1eady, <strong>Ch\u1ea5t n\u1ec1n nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e1t tri\u1ec3n c\u00e1c s\u1ea3n ph\u1ea9m y t\u1ebf v\u00e0 s\u1ea3n ph\u1ea9m y t\u1ebf d\u1ef1a tr\u00ean t\u1ebf b\u00e0o\/m\u00f4 c\u1ea7n \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n ki\u1ec3m tra\/ x\u00e9t nghi\u1ec7m mycoplasma th\u00edch h\u1ee3p.<\/strong><\/p>\n<p>Hi\u1ec7n c\u00f3 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u1ee9ng d\u1ee5ng ph\u00e1t hi\u1ec7n Mycoplasma nh\u01b0: nhu\u1ed9m m\u00f4 h\u1ecdc, quan s\u00e1t \u0111\u01b0\u1edbi k\u00ednh hi\u1ec3n vi \u0111i\u1ec7n t\u1eed (TEM, SEM), ph\u01b0\u01a1ng ph\u00e1p sinh h\u00f3a (th\u1eed nghi\u1ec7m d\u1ef1a tr\u00ean enzyme), <strong>ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y<\/strong>, <strong>ph\u01b0\u01a1ng ph\u00e1p nhu\u1ed9m hu\u1ef3nh quang DNA<\/strong> (DAPI stain), v\u00e0 ph\u01b0\u01a1ng ph\u00e1p khu\u1ebfch \u0111\u1ea1i axit nucleic (<strong>ph\u01b0\u01a1ng ph\u00e1p NAT<\/strong>) b\u1eb1ng k\u1ef9 thu\u1eadt PCR.<\/p>\n<p><strong>\u0110\u1ed1i v\u1edbi c\u00e1c s\u1ea3n ph\u1ea9m nu\u00f4i c\u1ea5y tr\u00ean 48 gi\u1edd, FDA y\u00eau c\u1ea7u x\u00e9t nghi\u1ec7m mycoplasma. USP \u0111\u00e3 ph\u00ea duy\u1ec7t quy tr\u00ecnh mycoplasma th\u01b0\u1eddng \u0111\u01b0\u1ee3c ch\u1ea5p nh\u1eadn \u1edf Hoa K\u1ef3, Nh\u1eadt B\u1ea3n v\u00e0 Li\u00ean minh Ch\u00e2u \u00c2u.<\/strong> <strong>FDA M\u1ef9 \u0111\u00e3 \u0111\u1ed3ng \u00fd v\u1edbi vi\u1ec7c s\u1eed d\u1ee5ng c\u00e1c ph\u01b0\u01a1ng ph\u00e1p thay th\u1ebf nh\u01b0 ph\u01b0\u01a1ng ph\u00e1p khu\u1ebfch \u0111\u1ea1i axit nucleic (ph\u01b0\u01a1ng ph\u00e1p NAT) b\u1eb1ng k\u1ef9 thu\u1eadt PCR, \u0111\u01b0\u1ee3c ch\u1ea5p nh\u1eadn b\u1edfi d\u01b0\u1ee3c \u0111i\u1ec3n Ch\u00e2u \u00c2u, Nh\u1eadt B\u1ea3n v\u00e0 Hoa K\u1ef3 ho\u1eb7c c\u00e1c x\u00e9t nghi\u1ec7m d\u1ef1a tr\u00ean enzyme (enzyme-based assays), nh\u01b0ng y\u00eau c\u1ea7u d\u1eef li\u1ec7u th\u1ea9m \u0111\u1ecbnh (validation data) \u0111\u1ec3 s\u1eed d\u1ee5ng ch\u00fang trong c\u00f4ng b\u1ed1 s\u1ea3n ph\u1ea9m<\/strong>.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"7_loai_chung_mycoplasma_duoc_phat_hien_theo_Duoc_dien_Nhat_Ban_Phien_ban_thu_17\"><\/span><strong>7 lo\u1ea1i ch\u1ee7ng mycoplasma \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n theo D\u01b0\u1ee3c \u0111i\u1ec3n Nh\u1eadt B\u1ea3n Phi\u00ean b\u1ea3n th\u1ee9 17<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>7 lo\u1ea1i ch\u1ee7ng \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ecdn t\u1eeb c\u00e1c quan \u0111i\u1ec3m sau \u0111\u00e2y l\u00e0 c\u00e1c ch\u1ee7ng mycoplasma c\u00f3 th\u1ec3 g\u00e2y \u00f4 nhi\u1ec5m ch\u1ea5t n\u1ec1n t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e1t tri\u1ec3n c\u00e1c s\u1ea3n ph\u1ea9m y t\u1ebf t\u1ea1i Nh\u1eadt B\u1ea3n.<\/p>\n<p>T\u1ea7n su\u1ea5t \u00f4 nhi\u1ec5m v\u00e0 quan \u0111i\u1ec3m ph\u00e1t sinh lo\u00e0i.<\/p>\n<p>C\u00e1c th\u00e0nh ph\u1ea7n c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb \u0111\u1ed9ng v\u1eadt \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o.<\/p>\n<figure class=\"table\">\n<table>\n<tbody>\n<tr>\n<td><strong>Ch\u1ee7ng mycoplasma<\/strong><\/td>\n<td><strong>V\u1eadt ch\u1ee7 t\u1ef1 nhi\u00ean<\/strong><\/td>\n<\/tr>\n<tr>\n<td><i>Mycoplasma hyorhinis<\/i><\/td>\n<td>L\u1ee3n<\/td>\n<\/tr>\n<tr>\n<td><i>Mycoplasma orale<\/i><\/td>\n<td>Ng\u01b0\u1eddi<\/td>\n<\/tr>\n<tr>\n<td><i>Mycoplasma pneumoniae<\/i><\/td>\n<td>Ng\u01b0\u1eddi<\/td>\n<\/tr>\n<tr>\n<td><i>Mycoplasma salivarium<\/i><\/td>\n<td>Ng\u01b0\u1eddi<\/td>\n<\/tr>\n<tr>\n<td><i>Acholeplasma laidlawii<\/i><\/td>\n<td>B\u00f2<\/td>\n<\/tr>\n<tr>\n<td><i>Mycoplasma fermentans<\/i><\/td>\n<td>Ng\u01b0\u1eddi<\/td>\n<\/tr>\n<tr>\n<td><i>Mycoplasma arginini<\/i><\/td>\n<td>B\u00f2\/ d\u00ea<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<h3><span class=\"ez-toc-section\" id=\"Phuong_phap_A_Phuong_phap_nuoi_cay_xet_nghiem_mycoplasma\"><\/span><strong>Ph\u01b0\u01a1ng ph\u00e1p A: Ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y x\u00e9t nghi\u1ec7m mycoplasma<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>C\u1ea5y m\u1eabu v\u00e0o m\u00f4i tr\u01b0\u1eddng (m\u00f4i tr\u01b0\u1eddng l\u1ecfng, m\u00f4i tr\u01b0\u1eddng th\u1ea1ch) v\u00e0 nu\u00f4i c\u1ea5y \u0111\u1ec3 ph\u00e1t hi\u1ec7n c\u00e1c khu\u1ea9n l\u1ea1c \u0111\u1eb7c tr\u01b0ng cho mycoplasma. <strong>Th\u1eddi gian t\u1eeb khi b\u1eaft \u0111\u1ea7u nu\u00f4i c\u1ea5y \u0111\u1ebfn khi k\u1ebft th\u00fac x\u00e9t nghi\u1ec7m l\u00e0 28 ng\u00e0y.<\/strong><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Phuong_phap_B_Phuong_phap_nhuom_huynh_quang_DNA\"><\/span><strong>Ph\u01b0\u01a1ng ph\u00e1p B: Ph\u01b0\u01a1ng ph\u00e1p nhu\u1ed9m hu\u1ef3nh quang DNA<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y gi\u00e1n ti\u1ebfp ph\u00e1t hi\u1ec7n mycoplasma \u0111\u00e3 ph\u00e1t tri\u1ec3n trong nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o b\u1eb1ng c\u00e1ch c\u1ea5y c\u00e1c t\u1ebf b\u00e0o ch\u1ec9 th\u1ecb (t\u1ebf b\u00e0o Vero) v\u1edbi m\u1eabu x\u00e9t nghi\u1ec7m v\u00e0 nhu\u1ed9m DNA c\u1ee7a mycoplasma b\u1eb1ng thu\u1ed1c nhu\u1ed9m hu\u1ef3nh quang \u0111\u1ec3 quan s\u00e1t c\u00e1c \u0111\u1ed1m hu\u1ef3nh quang nh\u1ecf ngo\u00e0i nh\u00e2n.<\/p>\n<p>Ph\u01b0\u01a1ng ph\u00e1p nhu\u1ed9m DNA c\u1ea7n chu\u1ea9n b\u1ecb c\u00e1c t\u1ebf b\u00e0o ch\u1ec9 th\u1ecb, r\u1ea5t kh\u00f3 \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 k\u1ebft qu\u1ea3 v\u00ec \u0111\u00e2y kh\u00f4ng ph\u1ea3i l\u00e0 ph\u01b0\u01a1ng ph\u00e1p d\u00e0nh ri\u00eang cho mycoplasma v\u00e0 c\u1ea7n k\u1ef9 thu\u1eadt vi\u00ean c\u00f3 kinh nghi\u1ec7m v\u00e0 k\u1ef9 n\u0103ng \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 m\u1eabu d\u01b0\u01a1ng t\u00ednh hay \u00e2m t\u00ednh.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Phuong_phap_C_Ky_thuat_khuech_dai_axit_nucleic_phuong_phap_NAT\"><\/span><strong>Ph\u01b0\u01a1ng ph\u00e1p C: K\u1ef9 thu\u1eadt khu\u1ebfch \u0111\u1ea1i axit nucleic (ph\u01b0\u01a1ng ph\u00e1p NAT)<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>Ly tr\u00edch DNA t\u1eeb m\u1eabu v\u00e0 khu\u1ebfch \u0111\u1ea1i ch\u00fang b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng m\u1ed3i \u0111\u1eb7c hi\u1ec7u cho mycoplasma \u0111\u1ec3 ph\u00e1t hi\u1ec7n mycoplasma.<\/p>\n<p><strong>Theo D\u01b0\u1ee3c \u0111i\u1ec3n Nh\u1eadt B\u1ea3n, Ch\u00e2u \u00c2u y\u00eau c\u1ea7u ph\u01b0\u01a1ng ph\u00e1p NAT c\u1ea7n \u0111\u01b0\u1ee3c th\u1ea9m \u0111\u1ecbnh (n\u1ebfu \u0111\u1ee7 \u0111\u1ed9 nh\u1ea1y \u0111\u01b0\u1ee3c x\u00e1c nh\u1eadn th\u00f4ng qua th\u1ea9m \u0111\u1ecbnh h\u1ee3p l\u1ec7), ph\u01b0\u01a1ng ph\u00e1p n\u00e0y c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng thay th\u1ebf cho Ph\u01b0\u01a1ng ph\u00e1p A (Ph\u01b0\u01a1ng \u00e1n thay th\u1ebf cho Ph\u01b0\u01a1ng ph\u00e1p A: C\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c 10CFU\/mL \u0111\u1ed1i v\u1edbi t\u1ea5t c\u1ea3 7 ch\u1ee7ng mycoplasma) v\u00e0 Ph\u01b0\u01a1ng ph\u00e1p B (Ph\u01b0\u01a1ng \u00e1n thay th\u1ebf cho Ph\u01b0\u01a1ng ph\u00e1p B: C\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c 10CFU\/mL \u0111\u1ed1i v\u1edbi t\u1ea5t c\u1ea3 7 ch\u1ee7ng mycoplasma).<\/strong><\/p>\n<p>K\u1ef9 thu\u1eadt khu\u1ebfch \u0111\u1ea1i axit nucleic (k\u1ef9 thu\u1eadt NAT) c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng nh\u01b0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p x\u00e9t nghi\u1ec7m mycoplasma nhanh ch\u00f3ng c\u00f3 \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u01b0\u1ee3t tr\u1ed9i, mi\u1ec5n l\u00e0 vi\u1ec7c th\u1ea9m \u0111\u1ecbnh h\u1ee3p l\u1ec7 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Bo_kit_MycoFinder_phat_hien_gen_Mycoplasma\"><\/span><strong>B\u1ed9 kit MycoFinder ph\u00e1t hi\u1ec7n gen Mycoplasma<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>MycoFinder s\u1eed d\u1ee5ng Real-Time PCR \u0111\u1ec3 ph\u00e1t hi\u1ec7n mycoplasma ch\u00ednh x\u00e1c, m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p x\u00e9t nghi\u1ec7m nh\u1ea1y, nhanh ch\u00f3ng, \u0111\u00e1ng tin c\u1eady v\u00e0 ti\u1ebft ki\u1ec7m chi ph\u00ed \u0111\u1ec3 ki\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng d\u01b0\u1ee3c ph\u1ea9m sinh h\u1ecdc v\u00e0 S\u1ea3n ph\u1ea9m Thu\u1ed1c tr\u1ecb li\u1ec7u Ti\u00ean ti\u1ebfn (ATMP).<\/p>\n<p>M\u1ed9t ki\u1ec3u x\u00e9t nghi\u1ec7m s\u00e1ng t\u1ea1o c\u1ee7a b\u1ed9 k\u00edt MycoFinder gi\u1ea3m thi\u1ec3u vi\u1ec7c x\u1eed l\u00fd m\u1eabu, d\u1ec5 d\u00e0ng chu\u1ea9n b\u1ecb m\u1eabu v\u00e0 gi\u1ea3m \u0111\u00e1ng k\u1ec3 th\u1eddi gian quay v\u00f2ng. D\u1ec5 s\u1eed d\u1ee5ng v\u00e0 th\u1ef1c hi\u1ec7n trong ph\u00f2ng th\u00ed nghi\u1ec7m, n\u01a1i th\u1eddi gian l\u00e0 r\u1ea5t quan tr\u1ecdng. MycoFinder tu\u00e2n th\u1ee7 D\u01b0\u1ee3c \u0111i\u1ec3n Ch\u00e2u \u00c2u, Hoa K\u1ef3 v\u00e0 Nh\u1eadt B\u1ea3n (EP 2.6.7, USP63 &amp; JP17).<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/WaQT1BGCjwstKqpxWsayeWZyHGlOUURyF9INtj9W.png\" alt=\"\" width=\"451\" height=\"338\"><\/p>\n<p>Th\u1ef1c hi\u1ec7n x\u00e9t nghi\u1ec7m mycoplasma nhanh:<\/p>\n<ul>\n<li>M\u1eabu t\u1eeb \u0111\u1ea7u \u0111\u1ebfn cu\u1ed1i cho k\u1ebft qu\u1ea3 d\u01b0\u1edbi 120 ph\u00fat.<\/li>\n<li>T\u00e1ch chi\u1ebft DNA trong 45 ph\u00fat. Chu\u1ea9n b\u1ecb PCR 4 b\u01b0\u1edbc \u0111\u01a1n gi\u1ea3n.<\/li>\n<li>Master Mix \u0111\u01b0\u1ee3c \u0111\u1ecbnh l\u01b0\u1ee3ng tr\u01b0\u1edbc gi\u00fap gi\u1ea3m thi\u1ec3u vi\u1ec7c x\u1eed l\u00fd m\u1eabu v\u00e0 chu tr\u00ecnh PCR (35-60 ph\u00fat).<\/li>\n<\/ul>\n<p>Tu\u00e2n th\u1ee7 d\u01b0\u1ee3c \u0111i\u1ec3n:<\/p>\n<ul>\n<li>Ho\u00e0n to\u00e0n tu\u00e2n th\u1ee7 D\u01b0\u1ee3c \u0111i\u1ec3n Ch\u00e2u \u00c2u, Nh\u1eadt B\u1ea3n v\u00e0 Hoa K\u1ef3 (EP 2.6.7, JP17, USP63) v\u1ec1 LOD, \u0111\u1ed9 m\u1ea1nh v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u.<\/li>\n<li>Gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n (LOD) d\u01b0\u1edbi 10 CFU\/mL.<\/li>\n<li>\u0110\u1eb7c hi\u1ec7u cao &#8211; ph\u00e1t hi\u1ec7n t\u1edbi 142 ch\u1ee7ng mycoplasma kh\u00e1c nhau (trong silico).<\/li>\n<\/ul>\n<p>Chi ph\u00ed x\u00e9t nghi\u1ec7m hi\u1ec7u qu\u1ea3:<\/p>\n<ul>\n<li>PCR strip format &#8211; ch\u1ec9 s\u1eed d\u1ee5ng s\u1ed1 l\u01b0\u1ee3ng d\u1ea3i\/\u1ed1ng c\u1ea7n thi\u1ebft cho m\u1ed7i l\u1ea7n ch\u1ea1y.<\/li>\n<li>C\u00e1c strip c\u00f2n l\u1ea1i c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u1edf +2 &#8211; 8\u00b0C trong t\u1ed1i \u0111a 12 th\u00e1ng.<\/li>\n<li>B\u1ed9 kit ch\u1ee9a 2 lo\u1ea1i PCR strip format (lo\u1ea1i trong su\u1ed1t v\u00e0 tr\u1eafng) cho \u0111\u1ecbnh d\u1ea1ng thi\u1ebft b\u1ecb linh ho\u1ea1t.<\/li>\n<\/ul>\n<h4><span class=\"ez-toc-section\" id=\"Cach_su_dung_bo_kit_phat_hien_gen_Mycoplasma_chuan_bi_thuoc_thu\"><\/span><strong>C\u00e1ch s\u1eed d\u1ee5ng b\u1ed9 kit ph\u00e1t hi\u1ec7n gen Mycoplasma (chu\u1ea9n b\u1ecb thu\u1ed1c th\u1eed)<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h4>\n<p>Kh\u00f4ng c\u1ea7n chu\u1ea9n b\u1ecb thu\u1ed1c th\u1eed v\u00ec thu\u1ed1c th\u1eed ph\u1ea3n \u1ee9ng kh\u00f4 \u0111\u01b0\u1ee3c \u0111\u1eb7t trong \u1ed1ng ph\u1ea3n \u1ee9ng cho m\u1ed7i \u1ed1ng (gi\u1ebfng qPCR).<\/p>\n<p>Khi s\u1eed d\u1ee5ng enzyme khu\u1ebfch \u0111\u1ea1i m\u1ea1nh, qu\u00e1 tr\u00ecnh ph\u00e1t hi\u1ec7n c\u00f3 th\u1ec3 ho\u00e0n t\u1ea5t trong v\u00f2ng m\u1ed9t gi\u1edd k\u1ec3 t\u1eeb khi b\u1eaft \u0111\u1ea7u khu\u1ebfch \u0111\u1ea1i.<\/p>\n<p>C\u00f3 th\u1ec3 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh gi\u1ea3 do nhi\u1ec5m b\u1ea9n m\u1eabu \u00e2m t\u00ednh b\u1eb1ng ki\u1ec3m so\u00e1t d\u01b0\u01a1ng t\u00ednh.<\/p>\n<p>B\u1ed9 kit n\u00e0y c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n mycoplasma trong qu\u00e1 tr\u00ecnh nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o \u21d2 N\u00f3 kh\u00f4ng ch\u1ec9 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ki\u1ec3m tra c\u00e1c s\u1ea3n ph\u1ea9m cu\u1ed1i c\u00f9ng m\u00e0 c\u00f2n \u0111\u1ec3 ki\u1ec3m tra t\u1ef1 nguy\u1ec7n trong qu\u00e1 tr\u00ecnh nu\u00f4i c\u1ea5y.<\/p>\n<p>Ghi ch\u00fa:<\/p>\n<ul>\n<li>\u0110\u00e1nh gi\u00e1 (c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 t\u1ef1 \u0111\u1ed9ng b\u1eb1ng ph\u1ea7n m\u1ec1m ph\u00e2n t\u00edch c\u1ee7a m\u00e1y real-time PCR)<\/li>\n<li>Probe b\u1ecb ph\u00e2n h\u1ee7y trong qu\u00e1 tr\u00ecnh PCR v\u00e0 t\u00edn hi\u1ec7u hu\u1ef3nh quang \u0111\u01b0\u1ee3c ph\u00e1t ra. B\u1eb1ng c\u00e1ch ph\u00e1t hi\u1ec7n t\u00edn hi\u1ec7u hu\u1ef3nh quang, c\u00f3 th\u1ec3 thu \u0111\u01b0\u1ee3c s\u1ef1 khu\u1ebfch \u0111\u1ea1i c\u1ee7a axit nucleic c\u1ee7a mycoplasma.<\/li>\n<\/ul>\n<h4><span class=\"ez-toc-section\" id=\"Phuong_phap_su_dung_qua_4_buoc_don_gian_de_thao_tac\"><\/span><strong>Ph\u01b0\u01a1ng ph\u00e1p s\u1eed d\u1ee5ng qua 4 b\u01b0\u1edbc (\u0111\u01a1n gi\u1ea3n, d\u1ec5 thao t\u00e1c):<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h4>\n<ul>\n<li>Cho 25\u03bcL m\u1eabu DNA x\u00e9t nghi\u1ec7m v\u1edbi n\u1ed3ng \u0111\u1ed9 th\u00edch h\u1ee3p v\u00e0o \u201ctest trip B\u201d.<\/li>\n<li>Sau khi h\u00f2a tan thu\u1ed1c th\u1eed r\u1eafn trong \u201ctest trip B\u201d b\u1eb1ng pipet, chuy\u1ec3n t\u1ea5t c\u1ea3 sang \u201ctest trip A\u201d.<\/li>\n<li>Sau khi h\u00f2a tan thu\u1ed1c th\u1eed r\u1eafn trong \u201ctest trip A\u201d b\u1eb1ng pipet, \u0111\u1eady n\u1eafp.<\/li>\n<li>Ph\u00e1t hi\u1ec7n b\u1eb1ng h\u1ec7 th\u1ed1ng real-time PCR.<\/li>\n<\/ul>\n<p><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/ypXP2wi1X7Z04pPslKlQxVryXewkl0wtFumBJf5D.png\" alt=\"\" width=\"624\" height=\"163\"><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Danh_gia_hieu_suat_cua_bo_kit_phat_hien_gen_Mycoplasma_va_tham_dinh_tuan_thu_duoc_dien_EP_JP\"><\/span><strong>\u0110\u00e1nh gi\u00e1 hi\u1ec7u su\u1ea5t c\u1ee7a b\u1ed9 kit ph\u00e1t hi\u1ec7n gen Mycoplasma v\u00e0 th\u1ea9m \u0111\u1ecbnh tu\u00e2n th\u1ee7 d\u01b0\u1ee3c \u0111i\u1ec3n EP, JP<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<h4><span class=\"ez-toc-section\" id=\"Tai_sao_tham_dinh_la_can_thiet\"><\/span><strong>T\u1ea1i sao th\u1ea9m \u0111\u1ecbnh l\u00e0 c\u1ea7n thi\u1ebft?<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h4>\n<p>Ng\u01b0\u1eddi d\u00f9ng c\u00f3 ph\u1ea3i th\u1ef1c hi\u1ec7n th\u1ea9m \u0111\u1ecbnh \u0111\u1ea7y \u0111\u1ee7 t\u1ea1i c\u01a1 s\u1edf c\u1ee7a h\u1ecd kh\u00f4ng? thao d\u01b0\u1ee3c \u0111i\u1ec3n JP17 \u201cKhi m\u1ed9t b\u1ed9 kit c\u00f3 s\u1eb5n tr\u00ean th\u1ecb tr\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ki\u1ec3m tra NAT, d\u1eef li\u1ec7u x\u00e1c th\u1ef1c \u0111\u1ea7y \u0111\u1ee7 t\u1eeb nh\u00e0 s\u1ea3n xu\u1ea5t c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng thay th\u1ebf cho d\u1eef li\u1ec7u x\u00e1c th\u1ef1c c\u1ee7a ng\u01b0\u1eddi d\u00f9ng\u201d. Do v\u1eady, n\u1ebfu c\u00f3 s\u1eb5n d\u1eef li\u1ec7u th\u1ea9m c\u1ee7a nh\u00e0 s\u1ea3n xu\u1ea5t, ng\u01b0\u1eddi d\u00f9ng kh\u00f4ng ph\u1ea3i th\u1ef1c hi\u1ec7n vi\u1ec7c th\u1ea9m \u0111\u1ecbnh \u0111\u1ea7y \u0111\u1ee7.<\/p>\n<p>Tuy nhi\u00ean, c\u00f3 s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 lo\u1ea1i t\u1ebf b\u00e0o c\u1ee7a m\u1eabu, ph\u01b0\u01a1ng ph\u00e1p ly tr\u00edch DNA, thi\u1ebft b\u1ecb real-time PCR, v.v. c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 l\u1eadp l\u1ea1i. Do \u0111\u00f3, c\u1ea7n ph\u1ea3i th\u1ef1c hi\u1ec7n th\u1ea9m \u0111\u1ecbnh hi\u1ec7u qu\u1ea3 c\u1ee7a b\u1ed9 k\u00edt t\u1ea1i c\u01a1 s\u1edf ng\u01b0\u1eddi d\u00f9ng.<\/p>\n<p>Ng\u01b0\u1eddi d\u00f9ng c\u1ea7n x\u00e1c nh\u1eadn r\u1eb1ng c\u00f3 th\u1ec3 nh\u1eadn \u0111\u01b0\u1ee3c k\u1ebft qu\u1ea3 th\u1ea9m \u0111\u1ecbnh gi\u1ed1ng nh\u01b0 k\u1ebft qu\u1ea3 do nh\u00e0 s\u1ea3n xu\u1ea5t tr\u00ecnh b\u00e0y t\u1ea1i c\u00e1c c\u01a1 s\u1edf c\u1ee7a ch\u00ednh h\u1ecd.<\/p>\n<h4><span class=\"ez-toc-section\" id=\"Huong_dan_tham_dinh_phuong_phap_NAT_trong_phat_hien_Mycoplasma_theo_duoc_dien_EP\"><\/span><strong>H\u01b0\u1edbng d\u1eabn th\u1ea9m \u0111\u1ecbnh ph\u01b0\u01a1ng ph\u00e1p NAT trong ph\u00e1t hi\u1ec7n Mycoplasma theo d\u01b0\u1ee3c \u0111i\u1ec3n EP:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h4>\n<ol>\n<li><strong>\u0110\u1ed9 \u0111\u1eb7c hi\u1ec7u:<\/strong> l\u00e0 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n \u0111\u00fang acid nucleic m\u1ee5c ti\u00eau khi c\u00f3 s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a nh\u1eefng th\u00e0nh ph\u1ea7n kh\u00f4ng mong \u0111\u1ee3i.<\/li>\n<\/ol>\n<p>\u0110\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p t\u00f9y thu\u1ed9c v\u00e0o s\u1ef1 l\u1ef1a ch\u1ecdn tr\u00ecnh t\u1ef1 m\u1ed3i v\u00e0 m\u1eabu d\u00f2 (probe) (\u0111\u1ec3 ph\u00e2n t\u00edch s\u1ea3n ph\u1ea9m cu\u1ed1i c\u00f9ng) v\u00e0 t\u00ednh nghi\u00eam ng\u1eb7t c\u1ee7a c\u00e1c \u0111i\u1ec1u ki\u1ec7n th\u1eed nghi\u1ec7m (\u1edf c\u1ea3 b\u01b0\u1edbc khu\u1ebfch \u0111\u1ea1i v\u00e0 ph\u00e1t hi\u1ec7n).<\/p>\n<p>C\u00e1c chi vi khu\u1ea9n Gram d\u01b0\u01a1ng c\u00f3 quan h\u1ec7 ph\u00e1t sinh lo\u00e0i g\u1ea7n v\u1edbi Mycoplasma l\u00e0 th\u00edch h\u1ee3p nh\u1ea5t \u0111\u01b0\u1ee3c d\u00f9ng \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p, bao g\u1ed3m Clostridium, Lactobacillus v\u00e0 Streptococcus.<\/p>\n<p>Tuy nhi\u00ean, \u0111\u00e2y ch\u01b0a ph\u1ea3i l\u00e0 danh s\u00e1ch \u0111\u1ea7y \u0111\u1ee7, v\u00e0 c\u00e1c lo\u00e0i \u0111\u01b0\u1ee3c ki\u1ec3m tra s\u1ebd ph\u1ee5 thu\u1ed9c v\u00e0o kh\u1ea3 n\u0103ng l\u00fd thuy\u1ebft (d\u1ef1a tr\u00ean tr\u00ecnh t\u1ef1 m\u1ed3i v\u00e0 m\u1eabu d\u00f2) c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ec3 ph\u00e1t hi\u1ec7n c\u00e1c lo\u00e0i kh\u00e1c.<\/p>\n<p>Trong tr\u01b0\u1eddng h\u1ee3p ph\u01b0\u01a1ng ph\u00e1p ph\u00e1t hi\u1ec7n acid nucleic c\u1ee7a vi khu\u1ea9n kh\u00f4ng ph\u1ea3i Mycoplasma, c\u1ea7n c\u00f3 chi\u1ebfn l\u01b0\u1ee3c th\u00edch h\u1ee3p \u0111\u1ec3 gi\u1ea3i th\u00edch k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh theo m\u1ed9t n\u1ec1n t\u1ea3ng quen thu\u1ed9c.<\/p>\n<ol>\n<li><strong>Gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n:<\/strong> l\u00e0 l\u01b0\u1ee3ng acid nucleic m\u1ee5c ti\u00eau th\u1ea5p nh\u1ea5t trong m\u1eabu c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c, kh\u00f4ng nh\u1ea5t thi\u1ebft ph\u1ea3i \u0111\u1ecbnh l\u01b0\u1ee3ng m\u1ed9t gi\u00e1 tr\u1ecb ch\u00ednh x\u00e1c.<\/li>\n<\/ol>\n<p>\u0110\u1ec3 x\u00e1c \u0111\u1ecbnh gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n, c\u1ea7n x\u00e1c \u0111\u1ecbnh \u0111i\u1ec3m gi\u1edbi h\u1ea1n d\u01b0\u01a1ng \u0111\u1ed1i v\u1edbi quy tr\u00ecnh ph\u00e2n t\u00edch v\u00e0 khu\u1ebfch \u0111\u1ea1i. \u0110i\u1ec3m gi\u1edbi h\u1ea1n d\u01b0\u01a1ng l\u00e0 s\u1ed1 l\u01b0\u1ee3ng b\u1ea3n sao tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau t\u1ed1i thi\u1ec3u tr\u00ean m\u1ed9t th\u1ec3 t\u00edch m\u1eabu c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n trong 95% s\u1ed1 l\u1ea7n ch\u1ea1y th\u1eed nghi\u1ec7m.<\/p>\n<p>\u0110i\u1ec3m gi\u1edbi h\u1ea1n d\u01b0\u01a1ng b\u1ecb \u1ea3nh h\u01b0\u1edfng b\u1edfi s\u1ed1 l\u01b0\u1ee3ng b\u1ed9 gene Mycoplasma trong c\u00e1c m\u1eabu ri\u00eang bi\u1ec7t \u0111ang ki\u1ec3m tra v\u00e0 c\u00e1c y\u1ebfu t\u1ed1 kh\u00e1c nh\u01b0 hi\u1ec7u qu\u1ea3 enzyme, c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn c\u00e1c gi\u00e1 tr\u1ecb ng\u01b0\u1ee1ng 95% kh\u00e1c nhau trong nh\u1eefng l\u1ea7n th\u1eed nghi\u1ec7m ph\u00e2n t\u00edch ri\u00eang l\u1ebb.<\/p>\n<p>\u0110\u1ec3 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c \u0111i\u1ec3m gi\u1edbi h\u1ea1n d\u01b0\u01a1ng, m\u1ed9t lo\u1ea1t c\u00e1c gi\u00e1 tr\u1ecb \u0111\u01b0\u1ee3c pha lo\u00e3ng c\u1ee7a m\u1eabu d\u01b0\u01a1ng t\u00ednh chu\u1ea9n \u0111\u01b0\u1ee3c th\u1eed nghi\u1ec7m v\u00e0o c\u00e1c ng\u00e0y kh\u00e1c nhau \u0111\u1ec3 ki\u1ec3m tra s\u1ef1 thay \u0111\u1ed5i gi\u1eefa c\u00e1c l\u1ea7n ch\u1ea1y th\u1eed nghi\u1ec7m.<\/p>\n<p>C\u00e1c lo\u00e0i \u0111\u1ea1i di\u1ec7n cho c\u00e1c l\u1ef1a ch\u1ecdn t\u1ed1i \u01b0u v\u1ec1 t\u1ea7n su\u1ea5t xu\u1ea5t hi\u1ec7n g\u00e2y \u00f4 nhi\u1ec5m, c\u0169ng nh\u01b0 m\u1ed1i quan h\u1ec7 ph\u00e1t sinh lo\u00e0i \u0111\u01b0\u1ee3c d\u00f9ng \u0111\u1ec3 x\u00e1c nh\u1eadn gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n l\u00e0:<\/p>\n<ul>\n<li><i>Acholeplasma laidlawii<\/i><\/li>\n<li><i>Mycoplasma fermentans<\/i><\/li>\n<li><i>M. hyorhinis<\/i><\/li>\n<li><i>M. orale<\/i><\/li>\n<li><i>M. pneumoniae ho\u1eb7c M. gallisepticum<\/i><\/li>\n<li><i>M. synoviae<\/i> (t\u00ecm th\u1ea5y trong qu\u00e1 tr\u00ecnh s\u1ea3n xu\u1ea5t c\u00f3 s\u1eed d\u1ee5ng nguy\u00ean v\u1eadt li\u1ec7u t\u1eeb gia c\u1ea7m);<\/li>\n<li><i>Mycoplasma arginini<\/i><\/li>\n<li><i>Spiroplasma citri <\/i>(t\u00ecm th\u1ea5y trong qu\u00e1 tr\u00ecnh s\u1ea3n xu\u1ea5t c\u00f3 s\u1eed d\u1ee5ng nguy\u00ean v\u1eadt li\u1ec7u t\u1eeb c\u00f4n tr\u00f9ng v\u00e0 th\u1ef1c v\u1eadt)<\/li>\n<\/ul>\n<p>\u0110\u1ed1i v\u1edbi m\u1ed7i ch\u1ee7ng, c\u1ea7n ki\u1ec3m tra \u00edt nh\u1ea5t 3 d\u00e3y pha lo\u00e3ng h\u00e0ng lo\u1ea1t 1\/10 l\u1ea7n trong c\u00e1c th\u1eed nghi\u1ec7m \u0111\u1ed9c l\u1eadp, v\u1edbi s\u1ed1 l\u1ea7n l\u1eb7p l\u1ea1i \u1edf m\u1ed7i \u0111\u1ed9 pha lo\u00e3ng sao cho cung c\u1ea5p t\u1ed5ng s\u1ed1 24 k\u1ebft qu\u1ea3 th\u1eed nghi\u1ec7m cho m\u1ed7i \u0111\u1ed9 pha lo\u00e3ng, cho ph\u00e9p ph\u00e2n t\u00edch th\u1ed1ng k\u00ea c\u00e1c k\u1ebft qu\u1ea3.<\/p>\n<p>\u0110\u1ec3 ki\u1ec3m so\u00e1t s\u1ed1 l\u01b0\u1ee3ng \u0111\u1ed9 pha lo\u00e3ng h\u00e0ng lo\u1ea1t, c\u1ea7n th\u1ef1c hi\u1ec7n th\u1eed nghi\u1ec7m s\u01a1 b\u1ed9 \u0111\u1ec3 nh\u1eadn bi\u1ebft gi\u00e1 tr\u1ecb gi\u1ea3 \u0111\u1ecbnh cho \u0111i\u1ec3m gi\u1edbi h\u1ea1n d\u01b0\u01a1ng (\u0111\u1ed9 pha lo\u00e3ng cao nh\u1ea5t cho t\u00edn hi\u1ec7u d\u01b0\u01a1ng t\u00ednh). Sau \u0111\u00f3 l\u1ef1a ch\u1ecdn ph\u1ea1m vi pha lo\u00e3ng xung quanh \u0111i\u1ec3m gi\u1edbi h\u1ea1n d\u01b0\u01a1ng gi\u1ea3 \u0111\u1ecbnh \u0111\u00f3. N\u1ed3ng \u0111\u1ed9 Mycoplasma (t\u00ednh theo CFU ho\u1eb7c b\u1ea3n sao) \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n trong 95% s\u1ed1 l\u1ea7n th\u1eed nghi\u1ec7m s\u1ebd \u0111\u01b0\u1ee3c t\u00ednh to\u00e1n b\u1eb1ng \u0111\u00e1nh gi\u00e1 th\u1ed1ng k\u00ea th\u00edch h\u1ee3p.<\/p>\n<p>Nh\u1eefng k\u1ebft qu\u1ea3 n\u00e0y c\u0169ng \u0111\u01b0\u1ee3c d\u00f9ng \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 s\u1ef1 bi\u1ebfn \u0111\u1ed5i c\u1ee7a quy tr\u00ecnh ph\u00e2n t\u00edch.<\/p>\n<ol>\n<li><strong>\u0110\u1ed9 ch\u1eafc ch\u1eafn<\/strong>: l\u00e0 th\u01b0\u1edbc \u0111o kh\u1ea3 n\u0103ng quy tr\u00ecnh kh\u00f4ng b\u1ecb \u1ea3nh h\u01b0\u1edfng b\u1edfi nh\u1eefng thay \u0111\u1ed5i nh\u1ecf (c\u00f3 ch\u1ee7 \u00fd) trong c\u00e1c tham s\u1ed1 ph\u01b0\u01a1ng ph\u00e1p v\u00e0 ch\u1ec9 ra v\u1ec1 \u0111\u1ed9 tin c\u1eady c\u1ee7a quy tr\u00ecnh trong qu\u00e1 tr\u00ecnh s\u1eed d\u1ee5ng th\u00f4ng th\u01b0\u1eddng.<\/li>\n<\/ol>\n<p>\u0110\u1ed1i v\u1edbi ph\u01b0\u01a1ng ph\u00e1p NAT, c\u00e1c bi\u1ebfn \u0111\u1ed5i nh\u1ecf c\u1ee7a c\u00e1c tham s\u1ed1 r\u1ea5t quan tr\u1ecdng, v\u00ed d\u1ee5 n\u1ed3ng \u0111\u1ed9 c\u1ee7a c\u00e1c th\u00e0nh ph\u1ea7n ph\u1ea3n \u1ee9ng khu\u1ebfch \u0111\u1ea1i (MgCl2, primer\/probe, dNTPs\u2026), ho\u1eb7c nh\u1eefng bi\u1ebfn \u0111\u1ed5i trong b\u1ed9 kit t\u00e1ch chi\u1ebft ho\u1eb7c quy tr\u00ecnh t\u00e1ch chi\u1ebft, c\u0169ng nh\u01b0 s\u1ef1 thay \u0111\u1ed5i c\u1ee7a c\u00e1c chu tr\u00ecnh nhi\u1ec7t kh\u00e1c nhau. T\u1ea5t c\u1ea3 c\u00e1c bi\u1ebfn \u0111\u1ed5i \u0111\u00f3 s\u1ebd \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 v\u00e0 t\u1ed5ng h\u1ee3p l\u1ea1i \u0111\u1ec3 \u0111\u01b0a ra k\u1ebft lu\u1eadn v\u1ec1 \u0111\u1ed9 ch\u1eafc ch\u1eafn c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p.<\/p>\n<h4><span class=\"ez-toc-section\" id=\"Nghien_cuu_ve_kha_nang_so_sanh_doi_voi_cac_phuong_phap_khac\"><\/span><strong>Nghi\u00ean c\u1ee9u v\u1ec1 kh\u1ea3 n\u0103ng so s\u00e1nh \u0111\u1ed1i v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p kh\u00e1c:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h4>\n<p>NAT c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng thay th\u1ebf cho c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ch\u00ednh th\u1ee9c (ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o ch\u1ec9 th\u1ecb v\u00e0\/ho\u1eb7c ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y vi khu\u1ea9n). Vi\u1ec7c so s\u00e1nh ph\u01b0\u01a1ng ph\u00e1p NAT v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ch\u00ednh th\u1ee9c ch\u1ee7 y\u1ebfu d\u1ef1a tr\u00ean gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p. Tuy nhi\u00ean, c\u0169ng n\u00ean xem x\u00e9t ch\u1ec9 ti\u00eau v\u1ec1 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u.<\/p>\n<p>\u0110\u1ed1i v\u1edbi ch\u1ec9 ti\u00eau gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n, ti\u00eau ch\u00ed \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 d\u1ef1a tr\u00ean:<\/p>\n<ul>\n<li>N\u1ebfu thay th\u1ebf cho ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y vi khu\u1ea9n, th\u00ec ph\u01b0\u01a1ng ph\u00e1p NAT ph\u1ea3i c\u00f3 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n 10 CFU\/ml cho m\u1ed7i lo\u00e0i th\u1eed nghi\u1ec7m trong m\u1ee5c 2.<\/li>\n<li>N\u1ebfu thay th\u1ebf cho ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o ch\u1ec9 th\u1ecb, th\u00ec ph\u01b0\u01a1ng ph\u00e1p NAT ph\u1ea3i c\u00f3 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n 100 CFU\/ml cho m\u1ed7i lo\u00e0i th\u1eed nghi\u1ec7m trong m\u1ee5c 2.<\/li>\n<li>C\u00f3 th\u1ec3 s\u1eed d\u1ee5ng 1 trong 2 c\u00e1ch sau \u0111\u1ec3 th\u1ef1c hi\u1ec7n nghi\u00ean c\u1ee9u so s\u00e1nh c\u00e1c ph\u01b0\u01a1ng ph\u00e1p:<\/li>\n<li>Th\u1ef1c hi\u1ec7n ph\u01b0\u01a1ng ph\u00e1p NAT song song v\u1edbi ph\u01b0\u01a1ng ph\u00e1p ch\u00ednh th\u1ee9c \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 \u0111\u1ed3ng th\u1eddi gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n tr\u00ean c\u00f9ng m\u1ed9t m\u1eabu chu\u1ea9n \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch.<\/li>\n<li>So s\u00e1nh hi\u1ec7u qu\u1ea3 c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p NAT b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng d\u1eef li\u1ec7u thu \u0111\u01b0\u1ee3c tr\u01b0\u1edbc \u0111\u00f3 t\u1eeb vi\u1ec7c th\u1ea9m \u0111\u1ecbnh ph\u01b0\u01a1ng ph\u00e1p ch\u00ednh th\u1ee9c. Trong tr\u01b0\u1eddng h\u1ee3p n\u00e0y, vi\u1ec7c chu\u1ea9n h\u00f3a c\u00e1c m\u1eabu chu\u1ea9n s\u1eed d\u1ee5ng trong vi\u1ec7c th\u1ea9m \u0111\u1ecbnh c\u1ea3 hai ph\u01b0\u01a1ng ph\u00e1p, c\u0169ng nh\u01b0 \u0111\u1ed9 \u1ed5n \u0111\u1ecbnh c\u1ee7a ch\u00fang ph\u1ea3i \u0111\u01b0\u1ee3c ghi ch\u00e9p c\u1ea9n th\u1eadn.<\/li>\n<\/ul>\n<p>C\u00e1c b\u00e1o c\u00e1o nghi\u00ean c\u1ee9u so s\u00e1nh c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u1ea3i \u0111\u01b0\u1ee3c m\u00f4 t\u1ea3 t\u1ea5t c\u1ea3 c\u00e1c ti\u00eau ch\u00ed th\u1ea9m \u0111\u1ecbnh (\u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u, gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n v\u00e0 s\u1ef1 bi\u1ebfn \u0111\u1ed5i, c\u0169ng nh\u01b0 \u0111\u1ed9 ch\u1eafc ch\u1eafn) \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 \u01b0u v\u00e0 nh\u01b0\u1ee3c \u0111i\u1ec3m c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p NAT thay th\u1ebf so v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ch\u00ednh th\u1ee9c.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Tai_lieu_tham_khao\"><\/span><i><strong>T\u00e0i li\u1ec7u tham kh\u1ea3o<\/strong><\/i><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ol>\n<li><i>Cellular Therapy: Principles, Methods, and Regulations. 2nd Edition. AABB. P. 26, 47, 205, 254, 469, 614-618, 858-859.<\/i><\/li>\n<li><i>T\u00e0i li\u1ec7u b\u1ed9 kit ph\u00e1t hi\u1ec7n gen Mycoplasma v\u00e0 th\u1ea9m \u0111\u1ecbnh tu\u00e2n th\u1ee7 d\u01b0\u1ee3c \u0111i\u1ec3n EP, JP. Nissui Pharmaceutical Co., Ltd.<\/i><\/li>\n<\/ol>\n","protected":false},"featured_media":5846,"parent":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-4364","service","type-service","status-publish","has-post-thumbnail","hentry","service-category-quy-trinh-huong-dan-su-dung"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>X\u00e9t nghi\u1ec7m Mycoplasma Trong S\u1ea3n Xu\u1ea5t T\u1ebf B\u00e0o<\/title>\n<meta name=\"description\" content=\"FDA M\u1ef9 \u0111\u00e3 \u0111\u1ed3ng \u00fd x\u00e9t nghi\u1ec7m mycoplasma b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p khu\u1ebfch \u0111\u1ea1i axit nucleic (ph\u01b0\u01a1ng ph\u00e1p NAT), c\u0169ng \u0111\u01b0\u1ee3c ch\u1ea5p nh\u1eadn b\u1edfi d\u01b0\u1ee3c \u0111i\u1ec3n M\u1ef9, Ch\u00e2u \u00c2u v\u00e0 Nh\u1eadt B\u1ea3n.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, 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