{"id":4412,"date":"2024-02-25T09:00:54","date_gmt":"2024-02-25T09:00:54","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/service\/how-to-prepare-stem-cell-cryopreservation-solution\/"},"modified":"2025-09-29T13:07:42","modified_gmt":"2025-09-29T06:07:42","slug":"cach-chuan-bi-dung-dich-bao-quan-dong-lanh-te-bao-goc","status":"publish","type":"service","link":"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cach-chuan-bi-dung-dich-bao-quan-dong-lanh-te-bao-goc\/","title":{"rendered":"C\u00e1ch Chu\u1ea9n B\u1ecb Dung D\u1ecbch B\u1ea3o Qu\u1ea3n \u0110\u00f4ng L\u1ea1nh T\u1ebf B\u00e0o G\u1ed1c"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cach-chuan-bi-dung-dich-bao-quan-dong-lanh-te-bao-goc\/#Bao_quan_dong_lanh_la_mot_phuong_phap_thuong_duoc_su_dung_de_luu_tru_dai_han_cac_te_bao_bao_gom_te_bao_goc_mau_cuong_ron_te_bao_goc_mau_ngoai_vi_PBMCs\" >B\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 l\u01b0u tr\u1eef d\u00e0i h\u1ea1n c\u00e1c t\u1ebf b\u00e0o, bao g\u1ed3m t\u1ebf b\u00e0o g\u1ed1c m\u00e1u cu\u1ed1ng r\u1ed1n, t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi (PBMCs).<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cach-chuan-bi-dung-dich-bao-quan-dong-lanh-te-bao-goc\/#Chuan_bi_dung_dich_bao_quan_dong_lanh_te_bao_goc_mau_cuong_ron\" >Chu\u1ea9n b\u1ecb dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh&nbsp;t\u1ebf b\u00e0o g\u1ed1c m\u00e1u cu\u1ed1ng r\u1ed1n<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cach-chuan-bi-dung-dich-bao-quan-dong-lanh-te-bao-goc\/#Chuan_Bi_Dung_Dich_Bao_Quan_Dong_Lanh_Te_Bao_Goc_Mau_Ngoai_Vi\" >Chu\u1ea9n B\u1ecb Dung D\u1ecbch B\u1ea3o Qu\u1ea3n \u0110\u00f4ng L\u1ea1nh&nbsp;T\u1ebf B\u00e0o G\u1ed1c M\u00e1u Ngo\u1ea1i Vi<\/a><\/li><\/ul><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"Bao_quan_dong_lanh_la_mot_phuong_phap_thuong_duoc_su_dung_de_luu_tru_dai_han_cac_te_bao_bao_gom_te_bao_goc_mau_cuong_ron_te_bao_goc_mau_ngoai_vi_PBMCs\"><\/span>B\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 l\u01b0u tr\u1eef d\u00e0i h\u1ea1n c\u00e1c t\u1ebf b\u00e0o, bao g\u1ed3m t\u1ebf b\u00e0o g\u1ed1c m\u00e1u cu\u1ed1ng r\u1ed1n, t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi (PBMCs).<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<h3><span class=\"ez-toc-section\" id=\"Chuan_bi_dung_dich_bao_quan_dong_lanh_te_bao_goc_mau_cuong_ron\"><\/span><strong>Chu\u1ea9n b\u1ecb dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh&nbsp;t\u1ebf b\u00e0o g\u1ed1c m\u00e1u cu\u1ed1ng r\u1ed1n<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>Qu\u00e1 tr\u00ecnh l\u01b0u tr\u1eef t\u1ebf b\u00e0o g\u1ed1c m\u00e1u cu\u1ed1ng r\u1ed1n \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n qua 2 b\u01b0\u1edbc ch\u00ednh: \u0111\u1ea7u ti\u00ean, \u0111\u01a1n v\u1ecb t\u1ebf b\u00e0o g\u1ed1c m\u00e1u cu\u1ed1ng r\u1ed1n \u0111\u01b0\u1ee3c h\u1ea1 nhi\u1ec7t \u0111\u1ed9 theo ch\u01b0\u01a1ng tr\u00ecnh, v\u00e0 sau \u0111\u00f3, \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh trong nit\u01a1 l\u1ecfng (\u1edf nhi\u1ec7t kho\u1ea3ng -196oC). \u0110\u1ec3 h\u1ea1n ch\u1ebf c\u00e1c t\u00e1c nh\u00e2n g\u00e2y \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn s\u1ef1 s\u1ed1ng c\u1ee7a t\u1ebf b\u00e0o g\u1ed1c trong l\u01b0u tr\u1eef d\u00e0i h\u1ea1n, m\u00f4i tr\u01b0\u1eddng b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u00e0 <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/xu-ly-bao-quan-te-bao-goc\/dung-dich-bao-quan-te-bao-goc\/dmso-bao-quan-te-bao.html\">dung d\u1ecbch DMSO<\/a> (Dimethyl Sulphoxide) \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn nh\u1ea5t.<\/p>\n<p><a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/xu-ly-bao-quan-te-bao-goc\/giai-phap-xu-ly-te-bao-goc-mau-cuong-ron-thu-cong\/bo-tui-xu-ly-mau-cuong-ron-thu-cong.html\">T\u00fai \u0111\u00f4ng l\u1ea1nh<\/a> (freezing bag) ch\u1ee9a 20 ml t\u1ebf b\u00e0o g\u1ed1c m\u00e1u cu\u1ed1ng r\u1ed1n sau x\u1eed l\u00fd, \u0111\u01b0\u1ee3c \u0111\u1eb7t tr\u00ean m\u00e1y Bio-mixer l\u1eafc 3 chi\u1ec1u v\u00e0 l\u00e0m m\u00e1t \u1edf nhi\u1ec7t \u0111\u1ed9 4-15\u00b0C (b\u1eb1ng c\u00e1ch \u0111\u1eb7t gi\u1eefa 2 g\u00f3i \u0111\u00e1 gel) tr\u01b0\u1edbc 10 ph\u00fat v\u00e0 trong qu\u00e1 tr\u00ecnh b\u01a1m dung d\u1ecbch b\u1ea3o qu\u1ea3n l\u1ea1nh \u0111\u00f4ng l\u1ea1nh t\u1ebf b\u00e0o.<\/p>\n<p>Th\u00f4ng th\u01b0\u1eddng, 5 ml dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh t\u1ebf b\u00e0o (\u0111\u01b0\u1ee3c chu\u1ea9n b\u1ecb s\u1eb3n theo h\u01b0\u1edbng d\u1eabn b\u00ean d\u01b0\u1edbi) \u0111\u01b0\u1ee3c b\u01a1m b\u00e0o t\u00fai t\u00fai \u0111\u00f4ng l\u1ea1nh \u0111\u1ec3 t\u1ea1o th\u00e0nh \u0111\u01a1n v\u1ecb t\u1ebf b\u00e0o g\u1ed1c m\u00e1u cu\u1ed1ng r\u1ed1n c\u00f3 t\u1ef7 l\u1ec7 m\u00e1u cu\u1ed1ng r\u1ed1n v\u00e0 dung d\u1ecbch b\u1ea3o qu\u1ea3n l\u1ea1nh l\u00e0 4:1.<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/PWBjLB4hvBzEPOxshUfZiOM8c91h57qgqvszh3zh.png\" alt=\"\" width=\"465\" height=\"286\"><\/p>\n<p><strong>\u0110\u1ec3 chu\u1ea9n b\u1ecb dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u1ed9ng l\u1ea1nh t\u1ebf b\u00e0o g\u1ed1c m\u00e1u cu\u1ed1ng r\u1ed1n (c\u00f3 th\u00e0nh ph\u1ea7n 50% DMSO (v\/v) v\u00e0 5% (w\/w) dextran 40):<\/strong><\/p>\n<p>Dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh ph\u1ea3i \u0111\u01b0\u1ee3c chu\u1ea9n b\u1ecb v\u00e0 s\u1eed d\u1ee5ng trong ng\u00e0y, ch\u1ee9a trong chai 150 mL c\u00f3 ghi s\u1ed1 lot v\u00e0 h\u1ea1n s\u1eed d\u1ee5ng c\u1ee7a c\u00e1c dung d\u1ecbch DMSO, Dextran 40. Tr\u1ecdng l\u01b0\u1ee3ng r\u1ed7ng c\u1ee7a chai \u0111\u01b0\u1ee3c c\u00e2n v\u00e0 ghi l\u00ean chai.<\/p>\n<p>Th\u1ec3 t\u00edch dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh c\u1ea7n d\u00f9ng trong ng\u00e0y ph\u1ee5 thu\u1ed9c v\u00e0o s\u1ed1 \u0111\u01a1n v\u1ecb t\u1ebf b\u00e0o g\u1ed1c c\u1ea7n \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh. Th\u00ed d\u1ee5: \u0111\u1ec3 chu\u1ea9n b\u1ecb 105 mL dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh, th\u1ef1c hi\u1ec7n c\u00e1c b\u01b0\u1edbc sau:<\/p>\n<ul>\n<li>D\u00f9ng \u1ed1ng ti\u00eam \u0111\u1ec3 h\u00fat 55 mL <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/xu-ly-bao-quan-te-bao-goc\/dung-dich-bao-quan-te-bao-goc\/dmso-bao-quan-te-bao.html\">dung d\u1ecbch DMSO<\/a> v\u00e0 b\u01a1m v\u00e0o chai 150 mL v\u00f4 tr\u00f9ng.<\/li>\n<li>X\u00e1c \u0111\u1ecbnh th\u1ec3 t\u00edch <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/xu-ly-bao-quan-te-bao-goc\/dung-dich-bao-quan-te-bao-goc\/dextran-bao-quan-te-bao.html\">Dextran 40<\/a> b\u1eb1ng c\u00e1ch c\u00e2n tr\u1ecdng l\u01b0\u1ee3ng chai ch\u1ee9a 55 mL dung d\u1ecbch DMSO tr\u1eeb tr\u1ecdng l\u01b0\u1ee3ng chai r\u1ed7ng, v\u00e0 sau \u0111\u00f3, chia 1.1.<\/li>\n<li>S\u1eed \u1ed1ng ti\u00eam th\u1ee9 hai h\u00fat th\u1ec3 t\u00edch dung d\u1ecbch Dextran 40 \u0111\u00e3 x\u00e1c \u0111\u1ecbnh, v\u00e0 th\u00eam v\u00e0o chai ch\u1ee9a dung d\u1ecbch DMSO.<\/li>\n<li>L\u1eafc tr\u1ed9n \u0111\u1ec1u chai dung d\u1ecbch b\u1ea3o qu\u1ea3n l\u1ea1nh (kho\u1ea3ng 4-5 l\u1ea7n) v\u00e0 l\u00e0m m\u00e1t dung d\u1ecbch b\u1ea3o qu\u1ea3n t\u1ebf b\u00e0o trong t\u1ee7 l\u1ea1nh (2-8\u00b0C) tr\u01b0\u1edbc khi s\u1eed d\u1ee5ng.<\/li>\n<\/ul>\n<h3><span class=\"ez-toc-section\" id=\"Chuan_Bi_Dung_Dich_Bao_Quan_Dong_Lanh_Te_Bao_Goc_Mau_Ngoai_Vi\"><\/span><strong>Chu\u1ea9n B\u1ecb Dung D\u1ecbch B\u1ea3o Qu\u1ea3n \u0110\u00f4ng L\u1ea1nh&nbsp;T\u1ebf B\u00e0o G\u1ed1c M\u00e1u Ngo\u1ea1i Vi<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>Khuy\u1ebfn ngh\u1ecb pha dung d\u1ecbch b\u1ea3o qu\u1ea3n t\u1ebf b\u00e0o theo th\u1ee9 t\u1ef1: Chu\u1ea9n b\u1ecb dung dich n\u01b0\u1edbc mu\u1ed1i trong m\u1ed9t falcon v\u00f4 tr\u00f9ng (th\u1ec3 t\u00edch 150ml), ti\u1ebfp theo, th\u00eam v\u00e0o dung d\u1ecbch HSA 20% (Human Serum Albumin 20%), v\u00e0 sau c\u00f9ng, dung d\u1ecbch DMSO \u0111\u01b0\u1ee3c th\u00eam v\u00e0o. <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/xu-ly-bao-quan-te-bao-goc\/dung-dich-bao-quan-te-bao-goc\/dmso-bao-quan-te-bao.html\">Dung d\u1ecbch DMSO<\/a> c\u1ea7n \u0111\u01b0\u1ee3c b\u01a1m t\u1eebng gi\u1ecdt v\u00e0 l\u1eafc \u0111\u1ec1u, nh\u1eb9 \u1ed1ng falcon (c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng m\u00e1y l\u1eafc Bio-mixer v\u00e0 b\u01a1m ti\u00eam \u0111i\u1ec7n s\u1ebd ti\u1ec7n l\u1ee3i, hi\u1ec7u qu\u1ea3 h\u01a1n) \u0111\u1ec3 tr\u00e1nh hi\u1ec7n t\u01b0\u1ee3ng k\u1ebft t\u1ee7a trong dung dich b\u1ea3o qu\u1ea3n l\u1ea1nh.<\/p>\n<p><strong>\u0110\u1ec3 chu\u1ea9n b\u1ecb 100 mL dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh cho 100 mL m\u1eabu t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi:<\/strong><\/p>\n<ul>\n<li>Chu\u1ea9n b\u1ecb 40mL dung d\u1ecbch n\u01b0\u1edbc mu\u1ed1i 0,9% (Normal Saline) trong \u1ed1ng falcon v\u00f4 tr\u00f9ng. Sau \u0111\u00f3, th\u00eam v\u00e0o 50mL dung d\u1ecbch HSA 20%. Sau c\u00f9ng, b\u01a1m 10 mL <a href=\"https:\/\/biogroupvietnam.com\/vi\/products\/xu-ly-bao-quan-te-bao-goc\/dung-dich-bao-quan-te-bao-goc\/dmso-bao-quan-te-bao.html\">dung d\u1ecbch DMSO<\/a>. Th\u1ec3 t\u00edch cu\u1ed1i thu \u0111\u01b0\u1ee3c l\u00e0 100mL, c\u00f3 n\u1ed3ng \u0111\u1ed9 5% DMSO v\u00e0 5% HSA.<\/li>\n<li>Cho dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh v\u1eeba chu\u1ea9n b\u1ecb \u1edf tr\u00ean v\u00e0o 100mL m\u1eabu t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi (\u0111\u00e3 bao g\u1ed3m ch\u1ea5t ch\u1ed1ng \u0111\u00f4ng), ngh\u0129a l\u00e0 t\u1ef7 l\u1ec7 1:1.<\/li>\n<\/ul>\n","protected":false},"featured_media":6052,"parent":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-4412","service","type-service","status-publish","has-post-thumbnail","hentry","service-category-quy-trinh-huong-dan-su-dung"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>C\u00e1ch Chu\u1ea9n B\u1ecb Dung D\u1ecbch B\u1ea3o Qu\u1ea3n \u0110\u00f4ng L\u1ea1nh T\u1ebf B\u00e0o G\u1ed1c<\/title>\n<meta name=\"description\" content=\"B\u1ea3o 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