{"id":4415,"date":"2024-02-25T09:01:51","date_gmt":"2024-02-25T09:01:51","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/service\/isolation-and-storage-of-peripheral-blood-hematopoietic-stem-cells-for-autotransplantation\/"},"modified":"2026-02-24T15:17:52","modified_gmt":"2026-02-24T08:17:52","slug":"phan-lap-va-luu-tru-te-bao-goc-tao-mau-tu-mau-ngoai-vi-de-ghep-tu-than","status":"publish","type":"service","link":"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/phan-lap-va-luu-tru-te-bao-goc-tao-mau-tu-mau-ngoai-vi-de-ghep-tu-than\/","title":{"rendered":"Ph\u00e2n L\u1eadp v\u00e0 L\u01b0u Tr\u1eef T\u1ebf B\u00e0o G\u1ed1c T\u1ea1o M\u00e1u T\u1eeb M\u00e1u Ngo\u1ea1i Vi \u0110\u1ec3 Gh\u00e9p T\u1ef1 Th\u00e2n"},"content":{"rendered":"<p>Gh\u00e9p t\u1ee7y x\u01b0\u01a1ng\u00a0l\u00e0 m\u1ed9t li\u1ec7u ph\u00e1p hi\u1ec7u qu\u1ea3 cho m\u1ed9t s\u1ed1 b\u1ec7nh r\u1ed1i lo\u1ea1n t\u1ee7y \u00e1c t\u00ednh v\u00e0 kh\u00f4ng \u00e1c t\u00ednh v\u00e0 \u0111\u01b0\u1ee3c cho l\u00e0 mang l\u1ea1i c\u01a1 h\u1ed9i s\u1ed1ng s\u00f3t l\u00e2u d\u00e0i t\u1ed1t nh\u1ea5t cho b\u1ec7nh nh\u00e2n m\u1eafc b\u1ec7nh b\u1ea1ch c\u1ea7u (leukemia) ho\u1eb7c u nguy\u00ean b\u00e0o th\u1ea7n kinh ti\u1ebfn tri\u1ec3n (advanced neuroblastoma) m\u00e0 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111i\u1ec1u tr\u1ecb kh\u00e1c \u0111\u00e3 th\u1ea5t b\u1ea1i. Tuy nhi\u00ean, th\u1eadt kh\u00f4ng may, ch\u1ec9 m\u1ed9t ph\u1ea7n ba trong s\u1ed1 nh\u1eefng b\u1ec7nh nh\u00e2n n\u00e0y c\u00f3 s\u1eb5n ng\u01b0\u1eddi hi\u1ebfn t\u1ee7y t\u01b0\u01a1ng th\u00edch v\u00e0 c\u00f3 r\u1ea5t \u00edt l\u1ef1a ch\u1ecdn \u0111i\u1ec1u tr\u1ecb cho nh\u1eefng ng\u01b0\u1eddi kh\u00f4ng c\u00f3, m\u1eb7c d\u00f9 hi\u1ec7n nay ng\u01b0\u1eddi ta \u0111ang c\u1ed1 g\u1eafng m\u1edf r\u1ed9ng nh\u00f3m ng\u01b0\u1eddi hi\u1ebfn t\u1ee7y b\u1eb1ng c\u00e1ch th\u00eam t\u1ee7y c\u1ee7a ng\u01b0\u1eddi hi\u1ebfn ph\u00f9 h\u1ee3p m\u1ed9t ph\u1ea7n.<\/p>\n<p>Do \u0111\u00f3, g\u1ea7n \u0111\u00e2y \u0111\u00e3 c\u00f3 nhi\u1ec1u m\u1ed1i quan t\u00e2m \u0111\u1ebfn vi\u1ec7c s\u1eed d\u1ee5ng t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi t\u1ef1 th\u00e2n (TBG MNV) \u0111\u1ec3 gh\u00e9p v\u00e0 c\u00e1c nguy\u00ean t\u1eafc c\u01a1 b\u1ea3n c\u1ee7a quy tr\u00ecnh n\u00e0y \u0111\u00e3 \u0111\u01b0\u1ee3c xem x\u00e9t r\u1ed9ng r\u00e3i. \u01afu \u0111i\u1ec3m c\u1ee7a vi\u1ec7c s\u1eed d\u1ee5ng PBSC t\u1ef1 th\u00e2n thay v\u00ec t\u1ee7y x\u01b0\u01a1ng l\u00e0 ch\u00fang c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c l\u1ea5y m\u00e0 kh\u00f4ng c\u1ea7n s\u1eed d\u1ee5ng thu\u1ed1c m\u00ea v\u00e0 kh\u00f4ng g\u00e2y kh\u00f3 ch\u1ecbu khi h\u00fat nhi\u1ec1u t\u1ee7y x\u01b0\u01a1ng. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y kh\u00f4ng b\u1ecb gi\u1edbi h\u1ea1n b\u1edfi \u0111\u1ed9 tu\u1ed5i c\u1ee7a b\u1ec7nh nh\u00e2n v\u00e0 n\u00f3 c\u00f3 kh\u1ea3 n\u0103ng \u00e1p d\u1ee5ng cho t\u1ea5t c\u1ea3 b\u1ec7nh nh\u00e2n m\u1eafc c\u00e1c r\u1ed1i lo\u1ea1n \u00e1c t\u00ednh. H\u01a1n n\u1eefa, kh\u1ea3 n\u0103ng l\u00e2y nhi\u1ec5m PBSC v\u1edbi c\u00e1c t\u1ebf b\u00e0o kh\u1ed1i u l\u00e0 th\u1ea5p. H\u01a1n n\u1eefa, TBG MNV\u00a0kh\u00f4i ph\u1ee5c c\u00e1c ch\u1ee9c n\u0103ng t\u1ea1o m\u00e1u v\u00e0 mi\u1ec5n d\u1ecbch nhanh h\u01a1n so v\u1edbi c\u00e1c t\u1ebf b\u00e0o g\u1ed1c t\u1ee7y, nh\u01b0 th\u1ec3 hi\u1ec7n trong c\u00e1c th\u00ed nghi\u1ec7m s\u1eed d\u1ee5ng s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o g\u1ed1c (CFU-GM) b\u1eb1ng nhau. Do \u0111\u00f3, vi\u1ec7c <strong>thu th\u1eadp v\u00e0 b\u1ea3o qu\u1ea3n l\u1ea1nh TBG MNV t\u1ef1 th\u00e2n\u00a0v\u00e0 sau \u0111\u00f3 gh\u00e9p ch\u00fang sau khi h\u00f3a tr\u1ecb li\u1ec1u cao (high-dose cytoreduction) d\u01b0\u1eddng nh\u01b0 l\u00e0 m\u1ed9t quy tr\u00ecnh h\u1eefu \u00edch \u0111\u1ec3 ch\u1eefa kh\u1ecfi b\u1ec7nh nh\u00e2n m\u1eafc<\/strong> <strong>c\u00e1c r\u1ed1i lo\u1ea1n \u00e1c t\u00ednh kh\u00f3 \u0111i\u1ec1u tr\u1ecb<\/strong> b\u1eb1ng c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111i\u1ec1u tr\u1ecb kh\u00e1c v\u00e0 kh\u00f4ng c\u00f3 s\u1eb5n t\u1ee7y x\u01b0\u01a1ng \u0111\u1ec3 gh\u00e9p.<\/p>\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/phan-lap-va-luu-tru-te-bao-goc-tao-mau-tu-mau-ngoai-vi-de-ghep-tu-than\/#Ky_thuat_xu_ly_te_bao_goc_mau_ngoai_vi_TBG_MNV\" >K\u1ef9 thu\u1eadt x\u1eed l\u00fd t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi (TBG MNV):<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/phan-lap-va-luu-tru-te-bao-goc-tao-mau-tu-mau-ngoai-vi-de-ghep-tu-than\/#Ky_thuat_luu_tru_te_bao_goc_mau_ngoai_vi\" >K\u1ef9 thu\u1eadt l\u01b0u tr\u1eef t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/phan-lap-va-luu-tru-te-bao-goc-tao-mau-tu-mau-ngoai-vi-de-ghep-tu-than\/#Ky_thuat_giai_dong_ra_dong_mau_te_bao_goc_mau_ngoai_vi_truoc_khi_ghep\" >K\u1ef9 thu\u1eadt gi\u1ea3i \u0111\u00f4ng (r\u00e3 \u0111\u00f4ng) m\u1eabu t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi tr\u01b0\u1edbc khi gh\u00e9p:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/phan-lap-va-luu-tru-te-bao-goc-tao-mau-tu-mau-ngoai-vi-de-ghep-tu-than\/#Tai_lieu_tham_khao\" >T\u00e0i li\u1ec7u tham kh\u1ea3o:<\/a><\/li><\/ul><\/nav><\/div>\n<h3><span class=\"ez-toc-section\" id=\"Ky_thuat_xu_ly_te_bao_goc_mau_ngoai_vi_TBG_MNV\"><\/span><strong>K\u1ef9 thu\u1eadt x\u1eed l\u00fd t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi (TBG MNV):<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>1)\u00a0Nh\u1eadn t\u00fai thu th\u1eadp MNV\u00a0t\u1eeb ph\u00f2ng thu nh\u1eadn (MNV\u00a0sau khi \u0111\u01b0\u1ee3c thu th\u1eadp t\u1eeb ng\u01b0\u1eddi cho b\u1eb1ng m\u00e1y chi\u1ebft t\u00e1ch t\u1ebf b\u00e0o t\u1ef1 \u0111\u1ed9ng). M\u1eabu \u0111\u01b0\u1ee3c v\u1eadn chuy\u1ec3n v\u00e0 \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u0111\u1ebfn khi x\u1eed l\u00fd \u1edf nhi\u1ec7t \u0111\u1ed9 4-15oC. Th\u1eddi gian x\u1eed l\u00fd t\u1ed1i \u0111a kh\u00f4ng qu\u00e1 48 gi\u1edd sau thu th\u1eadp.<\/p>\n<p>2)\u00a0\u0110\u1ecbnh l\u01b0\u1ee3ng to\u00e0n b\u1ed9 t\u00fai MNV\u00a0thu \u0111\u01b0\u1ee3c b\u1eb1ng c\u00e1ch c\u00e2n kh\u1ed1i l\u01b0\u1ee3ng (\u0111\u01a1n v\u1ecb gram), ghi l\u1ea1i k\u1ebft qu\u1ea3 \u0111\u1ec3 l\u1ea5y c\u01a1 s\u1edf t\u00e1ch huy\u1ebft t\u01b0\u01a1ng \u1edf b\u01b0\u1edbc 4.\u00a0T\u1ef7 l\u1ec7 quy \u0111\u1ed5i th\u1ec3 t\u00edch V (mL) t\u1eeb kh\u1ed1i l\u01b0\u1ee3ng m (gram) v\u1edbi kh\u1ed1i l\u01b0\u1ee3ng ri\u00eang \u03c1 (th\u01b0\u1eddng \u0111\u01b0\u1ee3c t\u00ednh 1.057):<\/p>\n<p style=\"text-align: center;\">V = m\/\u03c1<\/p>\n<p>V\u00ed d\u1ee5: C\u00e2n t\u00fai thu th\u1eadp \u0111\u01b0\u1ee3c 100gr, sau quy \u0111\u1ed5i: V = 100\/1.057 =\u00a094.61 mL<\/p>\n<p>3)\u00a0K\u1ebft n\u1ed1i t\u00fai thu th\u1eadp v\u00e0 t\u00fai chuy\u1ec3n b\u1eb1ng m\u00e1y n\u1ed1i d\u00e2y v\u00f4 tr\u00f9ng, sau \u0111\u00f3 \u0111\u1eb7t c\u00e1c t\u00fai v\u00e0o bucket 1,000mL c\u1ee7a m\u00e1y ly t\u00e2m l\u1ea1nh, \u0111\u1eb7t \u0111\u1ed1i x\u1ee9ng v\u00e0 c\u00e2n b\u1eb1ng\u00a0tr\u1ecdng l\u01b0\u1ee3ng (n\u1ebfu x\u1eed l\u00fd 1 m\u1eabu\/l\u1ea7n, ph\u1ea3i \u0111\u1eb7t v\u1eadt c\u00f9ng kh\u1ed1i l\u01b0\u1ee3ng v\u00e0o bucket \u0111\u1ed1i tr\u1ecdng) v\u00e0 c\u00e0i \u0111\u1eb7t ch\u01b0\u01a1ng tr\u00ecnh ly t\u00e2m \u1edf t\u1ed1c \u0111\u1ed9 1,400rpm trong 10 ph\u00fat, nhi\u1ec7t \u0111\u1ed9 ly t\u00e2m l\u00e0 10oC (ch\u01b0\u01a1ng tr\u00ecnh ly t\u00e2m n\u00e0y c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c di\u1ec1u ch\u1ec9nh t\u00f9y v\u00e0o \u0111i\u1ec1u ki\u1ec7n th\u1ef1c t\u1ebf c\u1ee7a ph\u00f2ng x\u1eed l\u00fd MNV).<\/p>\n<p><i>(M\u1ee5c \u0111\u00edch c\u1ee7a b\u01b0\u1edbc n\u00e0y nh\u1eb1m gi\u1ea3m th\u1ec3 t\u00edch l\u01b0u tr\u1eef t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi l\u00e0 t\u00e1ch b\u1ecf b\u1edbt l\u1edbp huy\u1ebft t\u01b0\u01a1ng (plasma) nh\u1eb1m ti\u1ebft ki\u1ec7m kh\u00f4ng gian b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh).<\/i><\/p>\n<p>4) Chuy\u1ec3n huy\u1ebft t\u01b0\u01a1ng sang t\u00fai chuy\u1ec3n\u00a0500mL (transfer bag) b\u1eb1ng b\u00e0n \u00e9p huy\u1ebft t\u01b0\u01a1ng, ph\u1ea7n dung d\u1ecbch t\u1ebf b\u00e0o c\u00f2n l\u1ea1i s\u1ebd \u0111\u01b0\u1ee3c l\u1ea5y m\u1eabu test l\u1ea7n 1 \u0111\u1ec3 ki\u1ec3m tra c\u00e1c ch\u1ec9 ti\u00eau theo quy \u0111\u1ecbnh. Th\u1ef1c hi\u1ec7n b\u1eb1ng c\u00e1ch k\u1ebft n\u1ed1i d\u1ee5ng c\u1ee5 l\u1ea5y m\u1eabu v\u00e0o c\u1ed5ng c\u1ee7a t\u00fai thu th\u1eadp v\u00e0 r\u00fat m\u1eabu v\u00e0o c\u00e1c \u1ed1ng vials 1,5mL (qu\u00e1 tr\u00ecnh thao t\u00e1c \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n trong ph\u00f2ng s\u1ea1ch v\u1edbi m\u1ee9c \u0111\u1ed9 an to\u00e0n sinh h\u1ecdc c\u1ea5p 2). Sau \u0111\u00f3, m\u1eabu test \u0111\u01b0\u1ee3c \u0111em \u0111i ki\u1ec3m tra:<\/p>\n<p>a. \u0110\u1ebfm s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o MNCs (\u0111\u01a1n v\u1ecb: t\u1ebf b\u00e0o\/mL):<\/p>\n<ul>\n<li>R\u00fat 20\u00b5L dung d\u1ecbch t\u1ebf b\u00e0o g\u1ed1c.<\/li>\n<li>Pha lo\u00e3ng v\u1edbi 380\u00b5L dung d\u1ecbch Natriclorua 0.9% (\u0111\u01b0\u1ee3c dung d\u1ecbch pha lo\u00e3ng 1\/20, t\u1ef7 l\u1ec7 dung d\u1ecbch TBG: dung d\u1ecbch Natriclorua 0.9% = 1:19).<\/li>\n<li>\u0110o v\u1edbi m\u00e1y \u0111\u1ebfm t\u1ed5ng ph\u00e2n t\u00edch t\u1ebf b\u00e0o t\u1ef1 \u0111\u1ed9ng ho\u00e0n to\u00e0n.<\/li>\n<li>\u0110o 3 l\u1ea7n \u0111\u1ec3 l\u1ea5y gi\u00e1 tr\u1ecb trung b\u00ecnh.<\/li>\n<\/ul>\n<p>b. \u0110\u1ebfm s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o CD34+:<\/p>\n<ul>\n<li>R\u00fat 30\u00b5L dung d\u1ecbch t\u1ebf b\u00e0o g\u1ed1c.<\/li>\n<li>\u0110\u1ebfm s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o b\u1eb1ng m\u00e1y \u0111\u1ebfm t\u1ebf b\u00e0o CD34+ ho\u1eb7c m\u00e1y Flow cytometry.<\/li>\n<li>\u0110o 3 l\u1ea7n \u0111\u1ec3 l\u1ea5y gi\u00e1 tr\u1ecb trung b\u00ecnh.<\/li>\n<\/ul>\n<p>c. Nu\u00f4i c\u1ea5y c\u1ee5m t\u1ebf b\u00e0o (CFU assay): th\u1ef1c nu\u00f4i c\u1ea5y tr\u00ean m\u00f4i tr\u01b0\u1eddng \u201cGold Standard\u201d MethoCult (tham kh\u1ea3o quy tr\u00ecnh chi ti\u1ebft trong t\u00e0i li\u1ec7u cung c\u1ea5p k\u00e8m).<\/p>\n<ul>\n<li>Chu\u1ea9n b\u1ecb m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y.<\/li>\n<li>Chu\u1ea9n b\u1ecb dung d\u1ecbch pha lo\u00e3ng TBG MNV.<\/li>\n<li>Chu\u1ea9n b\u1ecb \u0111\u0129a nu\u00f4i c\u1ea5y.<\/li>\n<li>Tr\u1ed9n m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y v\u00e0 d\u1ecbch pha lo\u00e3ng t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi theo t\u1ef7 l\u1ec7 khuy\u1ebfn ngh\u1ecb cho t\u1eebng th\u00ed nghi\u1ec7m mu\u1ed1n b\u1ed1 tr\u00ed.<\/li>\n<li>Ph\u00e2n ph\u1ed1i v\u00e0o \u0111\u0129a nu\u00f4i c\u1ea5y v\u00f4 tr\u00f9ng 35mm v\u00e0 l\u1ed3ng v\u00e0o \u0111\u0129a 100mm theo h\u01b0\u1edbng d\u1eabn.<\/li>\n<li>\u1ee6 \u1edf 37\u00b0C, 5% CO2, \u2265 95% \u1ea9m trong 7 ng\u00e0y \u0111\u1ed1i v\u1edbi m\u00f4i tr\u01b0\u1eddng MethoCult\u2122 Express v\u00e0 14 &#8211; 16 ng\u00e0y \u0111\u1ed1i v\u1edbi m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y MethoCult\u2122 kh\u00e1c.<\/li>\n<li>\u0110\u1ebfm k\u1ebft qu\u1ea3 sau 7 ng\u00e0y, 14 ng\u00e0y b\u1eb1ng k\u00ednh hi\u1ec3n vi soi ng\u01b0\u1ee3c ho\u1eb7c h\u1ec7 th\u1ed1ng \u0111\u1ebfm CFU t\u1ef1 \u0111\u1ed9ng.<\/li>\n<li>Tr\u01b0\u1eddng h\u1ee3p kh\u00f4ng \u0111\u1ebfm \u0111\u01b0\u1ee3c v\u00e0o ng\u00e0y quy \u0111\u1ecbnh, tham kh\u1ea3o th\u00eam c\u00e1ch x\u1eed l\u00fd trong t\u00e0i li\u1ec7u cung c\u1ea5p k\u00e8m.<\/li>\n<\/ul>\n<p>d. C\u1ea5y vi sinh (b\u1eb1ng h\u1ec7 th\u1ed1ng nu\u00f4i c\u1ea5y vi khu\u1ea9n t\u1ef1 \u0111\u1ed9ng).<\/p>\n<p>e. Huy\u1ebft \u0111\u1ed3 (b\u1eb1ng m\u00e1y ph\u00e2n t\u00edch huy\u1ebft h\u1ecdc).<\/p>\n<p>5) N\u1ebfu th\u1ec3 t\u00edch dung dich TBG MNV\u00a0thu \u0111\u01b0\u1ee3c nhi\u1ec1u h\u01a1n 100 mL, n\u00ean chia ra v\u00e0 b\u1ea3o qu\u1ea3n \u0111\u00f4ng s\u00e2u \u1edf c\u00e1c t\u00fai b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh CryoStore.<\/p>\n<p>6) Chu\u1ea9n b\u1ecb 40mL dung d\u1ecbch n\u01b0\u1edbc mu\u1ed1i 0,9% (Normal Saline) trong m\u1ed9t falcon v\u00f4 tr\u00f9ng. Sau \u0111\u00f3, th\u00eam v\u00e0o 50mL dung d\u1ecbch HSA 20%. Sau c\u00f9ng, b\u01a1m 10 mL <a href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/dung-dich-dmso-stemsol\/\">dung d\u1ecbch DMSO<\/a> (h\u00e0m l\u01b0\u1ee3ng &gt; 99%, Protide Pharmaceutical, M\u1ef9). Th\u1ec3 t\u00edch cu\u1ed1i thu \u0111\u01b0\u1ee3c l\u00e0 100mL, c\u00f3 n\u1ed3ng \u0111\u1ed9 5% DMSO v\u00e0 5% HAS.<\/p>\n<p>Cho dung d\u1ecbch b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh v\u1eeba chu\u1ea9n b\u1ecb \u1edf tr\u00ean v\u00e0o 100mL m\u1eabu t\u1ebf TBG MNV\u00a0(\u0111\u00e3 bao g\u1ed3m ch\u1ea5t ch\u1ed1ng \u0111\u00f4ng), ngh\u0129a l\u00e0 t\u1ef7 l\u1ec7 1:1.<\/p>\n<p>7) Chuy\u1ec3n dung d\u1ecbch t\u1ebf b\u00e0o g\u1ed1c sang t\u00fai b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh <a href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/tui-cryostore-bao-quan-te-bao\/\">CryoStore<\/a> b\u1eb1ng c\u00e1ch d\u00f9ng xi lanh (th\u1ec3 t\u00edch 60mL, c\u00f3 \u0111\u1ea7u luer lock).<\/p>\n<p>8) L\u1ea5y m\u1eabu test l\u1ea7n 2 \u0111\u1ec3 ki\u1ec3m tra c\u00e1c ch\u1ec9 ti\u00eau theo quy \u0111\u1ecbnh gi\u1ed1ng b\u01b0\u1edbc 4.<\/p>\n<p>(\u0110\u1ea3m b\u1ea3o s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o MNCs trong t\u00fai b\u1ea3o qu\u1ea3n ph\u1ea3i &lt; 1&#215;1010 t\u1ebf b\u00e0o\/t\u00fai)<\/p>\n<p>9) B\u01a1m dung dich b\u1ea3o qu\u1ea3n l\u1ea1nh (\u0111\u00e3 chu\u1ea9n b\u1ecb \u1edf b\u01b0\u1edbc 6) v\u00e0o t\u00fai b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh CryoStore (qua c\u1ed5ng female).<\/p>\n<p>10) T\u1ea1o c\u00e1c m\u1ed1i h\u00e0n (khuy\u1ebfn ngh\u1ecb <a href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/may-han-day-se470rs\/\">m\u00e1y h\u00e0n<\/a> \u0111\u01b0\u1ee3c v\u1eadt li\u1ec7u nh\u1ef1a EVO v\u00e0 EVA) v\u00e0 c\u1eaft b\u1ecf c\u00e1c \u0111o\u1ea1n d\u00e2y t\u00fai \u0111\u00e3 s\u1eed d\u1ee5ng \u0111\u1ec3 l\u00e0m g\u1ecdn ph\u1ea7n t\u00fai b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh.<\/p>\n<p>11)<a href=\"https:\/\/biogroupvietnam.com\/vi\/danh-muc-san-pham\/nhan-ma-vach-dong-lanh\/\"> D\u00e1n nh\u00e3n<\/a> v\u00e0 ghi th\u00f4ng tin t\u00fai b\u1ea3o qu\u1ea3n TBG (c\u00f3 th\u1ec3 c\u00e2n nh\u1eafc s\u1eed d\u1ee5ng qu\u1ea3n l\u00fd m\u1eabu b\u1eb1ng m\u00e3 v\u1ea1ch barcode \u0111\u00e1p \u1ee9ng ti\u00eau chu\u1ea9n ISBT 128).<\/p>\n<p>12) S\u1eed d\u1ee5ng <a href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/may-han-tui-overwrap\/\">m\u00e1y h\u00e0n t\u00fai overwrap<\/a> c\u00f3 ch\u1ee9c n\u0103ng h\u00e0n v\u00e0 \u0111\u1ea9y kh\u00ed \u0111\u1ec3 h\u00e0n k\u00edn t\u00fai bao ngo\u00e0i, nh\u1eb1m b\u1ea3o v\u1ec7 an to\u00e0n \u0111\u01a1n v\u1ecb t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Ky_thuat_luu_tru_te_bao_goc_mau_ngoai_vi\"><\/span><strong>K\u1ef9 thu\u1eadt l\u01b0u tr\u1eef t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ol>\n<li>\u0110\u1eb7t t\u00fai \u0111\u00f4ng l\u1ea1nh t\u1ebf b\u00e0o \u0111\u00e3 \u0111\u01b0\u1ee3c h\u00e0n k\u00edn trong t\u00fai overwrap v\u00e0o h\u1ed9p cassette\u00a0ph\u00f9 h\u1ee3p v\u00e0 d\u00e1n nh\u00e3n (nh\u00e3n m\u00e3 v\u1ea1ch). T\u1ea1o n\u00ean c\u00e1c \u0111\u01a1n v\u1ecb TBG MNV ti\u00eau chu\u1ea9n.<\/li>\n<li>\u0110\u1eb7t cassette\u00a0(ch\u1ee9a \u0111\u01a1n v\u1ecb t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi) v\u00e0o <a href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/icecube-14s\/\">thi\u1ebft b\u1ecb h\u1ea1 nhi\u1ec7t \u0111\u1ed9 theo ch\u01b0\u01a1ng tr\u00ecnh<\/a> (CRF) v\u1edbi ch\u01b0\u01a1ng tr\u00ecnh h\u1ea1 nhi\u1ec7t \u0111\u1ed9 \u0111\u00e3 \u0111\u01b0\u1ee3c l\u1eadp tr\u00ecnh.<\/li>\n<\/ol>\n<p>Khi qu\u00e1 tr\u00ecnh h\u1ea1 nhi\u1ec7t m\u1eabu k\u1ebft th\u00fac, nhi\u1ec7t \u0111\u1ed9 t\u00fai b\u1ea3o qu\u1ea3n t\u1ebf b\u00e0o g\u1ed1c kho\u1ea3ng -90 \u0111\u1ebfn -160oC.<\/p>\n<ol>\n<li>Sau khi h\u1ea1 nhi\u1ec7t \u0111\u1ed9 m\u1eabu, nhanh ch\u00f3ng chuy\u1ec3n cassette\u00a0v\u00e0o gi\u00e1 \u0111\u1ee1 canister v\u00e0 \u0111\u1eb7t v\u00e0o frame. Sau \u0111\u00f3, ti\u1ebfn h\u00e0nh l\u01b0u tr\u1eef trong <a href=\"https:\/\/biogroupvietnam.com\/vi\/san-pham\/binh-mve-heco-800\/\">b\u00ecnh l\u01b0u tr\u1eef m\u1eabu<\/a>\u00a0(\u1edf nhi\u1ec7t \u0111\u1ed9 -196oC) c\u00e0ng nhanh c\u00e0ng t\u1ed1t (\u0111\u1ec3 tr\u00e1nh hi\u1ec7n t\u01b0\u1ee3ng gia nhi\u1ec7t t\u1ea1m th\u1eddi c\u1ee7a m\u1eabu &#8211; TWEs). Qu\u00e1 tr\u00ecnh l\u01b0u tr\u1eef \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n, b\u1ea3o qu\u1ea3n cho \u0111\u1ebfn khi c\u00f3 ch\u1ec9 \u0111\u1ecbnh s\u1eed d\u1ee5ng \u0111\u01a1n v\u1ecb TBG MNV.<\/li>\n<\/ol>\n<p><i>(Ghi ch\u00fa: ghi nh\u1eadn th\u00f4ng tin m\u1eabu, ng\u00e0y l\u01b0u, v\u1ecb tr\u00ed l\u01b0u m\u1eabu trong thi\u1ebft b\u1ecb l\u01b0u tr\u1eef \u0111\u00f4ng l\u1ea1nh \u0111\u1ec3 thu\u1eadn ti\u1ec7n cho qu\u00e1 tr\u00ecnh l\u1ea5y m\u1eabu gh\u00e9p).\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/i><\/p>\n<p>Ngu\u1ed3n c\u1ea5p nit\u01a1 l\u1ecfng ph\u1ea3i \u0111\u01b0\u1ee3c cung c\u1ea5p \u0111\u1ea7y \u0111\u1ee7, li\u00ean t\u1ee5c v\u00e0 \u1ed5n \u0111\u1ecbnh trong su\u1ed1t qu\u00e1 tr\u00ecnh l\u01b0u tr\u1eef, nh\u1eb1m \u0111\u1ea3m b\u1ea3o ch\u1ea5t l\u01b0\u1ee3ng c\u00e1c \u0111\u01a1n v\u1ecb TBG MNV\u00a0ch\u1ee9a trong b\u00ecnh l\u01b0u tr\u1eef m\u1eabu).<\/p>\n<ol>\n<li>Th\u1eddi gian l\u01b0u tr\u1eef c\u00f3 th\u1ec3 l\u00ean \u0111\u1ebfn 18 n\u0103m. Tuy nhi\u00ean, hi\u1ec7n t\u1ea1i gi\u1edbi h\u1ea1n v\u1ec1 th\u1eddi gian b\u1ea3o qu\u1ea3n m\u1eabu t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi v\u1eabn ch\u01b0a \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh ch\u00ednh th\u1ee9c.<\/li>\n<\/ol>\n<h3><span class=\"ez-toc-section\" id=\"Ky_thuat_giai_dong_ra_dong_mau_te_bao_goc_mau_ngoai_vi_truoc_khi_ghep\"><\/span><strong>K\u1ef9 thu\u1eadt gi\u1ea3i \u0111\u00f4ng (r\u00e3 \u0111\u00f4ng) m\u1eabu t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi tr\u01b0\u1edbc khi gh\u00e9p:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ol>\n<li>L\u1ea5y canister ch\u1ee9a t\u00fai t\u1ebf b\u00e0o g\u1ed1c m\u00e1u ngo\u1ea1i vi ra kh\u1ecfi h\u1ec7 th\u1ed1ng b\u1ea3o qu\u1ea3n t\u1ebf b\u00e0o g\u1ed1c (tank nit\u01a1 l\u1ecfng l\u01b0u tr\u1eef t\u1ebf b\u00e0o g\u1ed1c).<\/li>\n<li>Ki\u1ec3m tra \u0111\u00fang th\u00f4ng tin m\u1eabu, b\u1ec7nh nh\u00e2n.<\/li>\n<li>T\u00fai t\u1ebf b\u00e0o g\u1ed1c \u0111\u01b0\u1ee3c r\u00e3 \u0111\u00f4ng trong b\u00ecnh \u0111i\u1ec1u nhi\u1ec7t n\u01b0\u1edbc 37oC (s\u1eed d\u1ee5ng b\u1ec3 water bath) c\u00f3 khu\u1ea5y \u0111\u1ed1i l\u01b0u ho\u1eb7c <a href=\"https:\/\/biogroupvietnam.com\/vi\/danh-muc-san-pham\/may-ra-dong-te-bao\/\">m\u00e1y r\u00e3 \u0111\u00f4ng t\u1ef1 \u0111\u1ed9ng<\/a> (c\u00f3 l\u1eadp tr\u00ecnh).<\/li>\n<li>L\u1ea5y m\u1eabu test l\u1ea7n 3 \u0111\u1ec3 ki\u1ec3m tra t\u1ef7 l\u1ec7 s\u1ed1ng\/ch\u1ebft, kh\u1ea3 n\u0103ng bi\u1ec7t h\u00f3a t\u1ebf b\u00e0o sau qu\u00e1 tr\u00ecnh b\u1ea3o qu\u1ea3n trong nit\u01a1 l\u1ecfng tr\u01b0\u1edbc khi b\u00e0n giao t\u00fai TBG cho ph\u00f2ng gh\u00e9p.<\/li>\n<li>N\u1ebfu c\u1ea7n, th\u1ef1c hi\u1ec7n r\u1eeda lo\u1ea1i b\u1ecf dung d\u1ecbch ch\u1ea5t b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh (dung d\u1ecbch DMSO) tr\u01b0\u1edbc khi gh\u00e9p (c\u00f3 th\u1ec3 \u00e1p d\u1ee5ng \u1edf m\u1ed9t s\u1ed1 ph\u00f2ng Lab).<\/li>\n<\/ol>\n<p><i>Ghi ch\u00fa: t\u200b\u200b\u200b\u200b\u200b\u200b\u00e0i li\u1ec7u k\u1ef9 thu\u1eadt n\u00e0y mang t\u00ednh ch\u1ea5t tham kh\u1ea3o, c\u1ea7n ph\u1ea3i th\u1ea9m \u0111\u1ecbnh hi\u1ec7u qu\u1ea3 quy tr\u00ecnh th\u1ef1c hi\u1ec7n t\u1ea1i c\u01a1 s\u1edf c\u1ee7a ng\u01b0\u1eddi d\u00f9ng, v\u00ec c\u00f3\u00a0s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 ph\u01b0\u01a1ng ph\u00e1p, th\u1ec3 t\u00edch x\u1eed l\u00fd\u00a0m\u00e1u ngo\u1ea1i vi, thi\u1ebft b\u1ecb, v.v. \u0110\u1ec3 \u0111\u01b0\u1ee3c h\u1ed5 tr\u1ee3, h\u00e3y\u00a0<\/i><a href=\"https:\/\/biogroupvietnam.com\/vi\/lien-he\/\"><i>li\u00ean h\u1ec7<\/i><\/a><i>\u00a0v\u1edbi ch\u00fang t\u00f4i.<\/i><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Tai_lieu_tham_khao\"><\/span><i><strong>T\u00e0i li\u1ec7u tham kh\u1ea3o:<\/strong><\/i><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li>Veeraputhiran, Muthu; Theus, John W.; Pesek, Gina; Barlogie, Bart; Cottler-Fox, Michele (2010). Viability and engraftment of hematopoietic progenitor cells after long-term cryopreservation: effect of diagnosis and percentage dimethyl sulfoxide concentration. Cytotherapy, 12(6), 764\u2013766. doi:10.3109\/14653241003745896. Original scientific paper.<\/li>\n<li>Y Takaue, T Watanabe, Y Kawano, T Koyama, T Abe, T Suzue, J Satoh, T Shimokawa, T Ninomiya and M Kosaka. Isolation and storage of peripheral blood hematopoietic stem cells for autotransplantation into children with cancer. bloodjournal.hematologylibrary.org. 1989 74: 1245-1251.<\/li>\n<\/ul>\n","protected":false},"featured_media":5909,"parent":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-4415","service","type-service","status-publish","has-post-thumbnail","hentry","service-category-quy-trinh-huong-dan-su-dung"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Ph\u00e2n L\u1eadp v\u00e0 L\u01b0u Tr\u1eef T\u1ebf B\u00e0o G\u1ed1c T\u1ea1o M\u00e1u T\u1eeb M\u00e1u Ngo\u1ea1i Vi \u0110\u1ec3 Gh\u00e9p T\u1ef1 Th\u00e2n<\/title>\n<meta name=\"description\" content=\"K\u1ef9 thu\u1eadt ph\u00e2n l\u1eadp v\u00e0 l\u01b0u tr\u1eef t\u1ebf b\u00e0o g\u1ed1c t\u1ea1o m\u00e1u ngo\u1ea1i vi\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, 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