{"id":4425,"date":"2025-01-07T04:42:07","date_gmt":"2025-01-07T04:42:07","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/service\/how-are-cells-in-a-multilayer-cell-culture-vessel-harvested\/"},"modified":"2025-09-29T13:07:26","modified_gmt":"2025-09-29T06:07:26","slug":"te-bao-trong-binh-nuoi-cay-te-bao-nhieu-tang-duoc-thu-hoach-nhu-the-nao","status":"publish","type":"service","link":"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/te-bao-trong-binh-nuoi-cay-te-bao-nhieu-tang-duoc-thu-hoach-nhu-the-nao\/","title":{"rendered":"T\u1ebf B\u00e0o Trong B\u00ecnh Nu\u00f4i C\u1ea5y T\u1ebf B\u00e0o Nhi\u1ec1u T\u1ea7ng \u0110\u01b0\u1ee3c Thu Ho\u1ea1ch Nh\u01b0 Th\u1ebf N\u00e0o?"},"content":{"rendered":"

C\u00e1c b\u01b0\u1edbc \u0111\u1ec3 thu ho\u1ea1ch t\u1ebf b\u00e0o trong <\/strong>b\u00ecnh nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o nhi\u1ec1u t\u1ea7ng<\/strong><\/a><\/h2>\n

1. Lo\u1ea1i b\u1ecf m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y.<\/p>\n

2. R\u1eeda s\u1ea1ch l\u1edbp t\u1ebf b\u00e0o \u0111\u01a1n.<\/p>\n

a. Th\u00eam th\u1ec3 t\u00edch khuy\u1ebfn ngh\u1ecb (40 \u0111\u1ebfn 50 mL m\u1ed7i l\u1edbp) CMF-PBS v\u00e0o bu\u1ed3ng. \u0110\u1eady n\u1eafp ch\u1eb7t v\u00e0 ph\u00e2n ph\u1ed1i CMF-PBS \u0111\u1ec1u v\u00e0o m\u1ed7i l\u1edbp bu\u1ed3ng b\u1eb1ng c\u00e1ch \u0111\u1eb7t bu\u1ed3ng n\u1eb1m nghi\u00eang v\u1ec1 ph\u00eda d\u00e0i nh\u1ea5t.<\/p>\n

b. Khi ch\u1ea5t l\u1ecfng \u0111\u00e3 c\u00e2n b\u1eb1ng, d\u1ef1ng \u0111\u1ee9ng b\u00ecnh \u0111\u1ec3 t\u00e1ch c\u00e1c l\u1edbp, sau \u0111\u00f3 t\u1eeb t\u1eeb \u0111\u1eb7t bu\u1ed3ng xu\u1ed1ng v\u1ecb tr\u00ed \u1ee7. Nghi\u00eang bu\u1ed3ng qua l\u1ea1i theo c\u1ea3 hai h\u01b0\u1edbng \u0111\u1ec3 r\u1eeda s\u1ea1ch k\u1ef9 l\u01b0\u1ee1ng t\u1eebng l\u1edbp v\u00e0 lo\u1ea1i b\u1ecf to\u00e0n b\u1ed9 m\u00f4i tr\u01b0\u1eddng c\u0169.<\/p>\n

c. L\u1ea5y ra v\u00e0 lo\u1ea1i b\u1ecf dung d\u1ecbch r\u1eeda. Khuy\u1ebfn ngh\u1ecb r\u1eeda l\u1ea7n th\u1ee9 hai b\u1eb1ng CMF-PBS \u0111\u1ed1i v\u1edbi c\u00e1c t\u1ebf b\u00e0o kh\u00f3 thu ho\u1ea1ch.<\/p>\n

3. T\u00e1ch l\u1edbp \u0111\u01a1n.<\/p>\n

a. Th\u00eam th\u1ec3 t\u00edch khuy\u1ebfn ngh\u1ecb (20 \u0111\u1ebfn 30 mL m\u1ed7i l\u1edbp) dung d\u1ecbch t\u00e1ch \u0111\u00e3 \u0111\u01b0\u1ee3c l\u00e0m \u1ea5m tr\u01b0\u1edbc v\u00e0o bu\u1ed3ng v\u00e0 ph\u00e2n ph\u1ed1i \u0111\u1ec1u cho m\u1ed7i bu\u1ed3ng b\u1eb1ng c\u00e1ch l\u00e0m theo c\u00e1c b\u01b0\u1edbc r\u1eeda. L\u00e0m \u1ea5m tr\u01b0\u1edbc dung d\u1ecbch t\u00e1ch s\u1ebd l\u00e0m gi\u1ea3m th\u1eddi gian ti\u1ebfp x\u00fac c\u1ea7n thi\u1ebft.<\/p>\n

b. G\u00f5 nh\u1eb9 v\u00e0o th\u00e0nh bu\u1ed3ng c\u00f3 th\u1ec3 gi\u00fap t\u00e1ch t\u1ebf b\u00e0o kh\u1ecfi b\u1ec1 m\u1eb7t.<\/p>\n

4. Nghi\u00eang nh\u1eb9 bu\u1ed3ng sang hai b\u00ean v\u00e0 t\u1eeb \u0111\u1ea7u n\u00e0y sang \u0111\u1ea7u kia \u0111\u1ec3 ph\u00e2n ph\u1ed1i \u0111\u1ec1u dung d\u1ecbch t\u00e1ch tr\u00ean m\u1ed7i l\u1edbp. G\u00f5 nh\u1eb9 v\u00e0o th\u00e0nh bu\u1ed3ng c\u00f3 th\u1ec3 gi\u00fap t\u00e1ch t\u1ebf b\u00e0o kh\u1ecfi b\u1ec1 m\u1eb7t.<\/p>\n

Khuy\u1ebfn ngh\u1ecb: N\u00ean nu\u00f4i c\u00f9ng m\u1ed9t t\u1ebf b\u00e0o trong \u0111i\u1ec1u ki\u1ec7n gi\u1ed1ng h\u1ec7t nhau (m\u1eadt \u0111\u1ed9 t\u1ebf b\u00e0o v\u00e0 l\u01b0\u1ee3ng m\u00f4i tr\u01b0\u1eddng t\u01b0\u01a1ng \u0111\u1ed1i) trong b\u00ecnh nu\u00f4i c\u1ea5y \u0111\u1ed1i ch\u1ee9ng, v\u00ec kh\u00f4ng th\u1ec3 theo d\u00f5i tr\u1ef1c ti\u1ebfp t\u00e1c \u0111\u1ed9ng c\u1ee7a dung d\u1ecbch t\u00e1ch l\u00ean c\u00e1c t\u1ebf b\u00e0o trong bu\u1ed3ng. Sau \u0111\u00f3, b\u00ecnh nu\u00f4i c\u1ea5y \u0111\u1ed1i ch\u1ee9ng n\u00e0y (th\u01b0\u1eddng l\u00e0 b\u00ecnh ho\u1eb7c bu\u1ed3ng 1 l\u1edbp) c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c x\u1eed l\u00fd song song \u0111\u1ec3 theo d\u00f5i c\u1ea3 s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a t\u1ebf b\u00e0o v\u00e0 ti\u1ebfn tr\u00ecnh c\u1ee7a quy tr\u00ecnh thu ho\u1ea1ch t\u1ebf b\u00e0o.<\/p>\n

5. Thu th\u1eadp dung d\u1ecbch t\u00e1ch ch\u1ee9a c\u00e1c t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c t\u00e1i huy\u1ec1n ph\u00f9 trong \u1ed1ng ly t\u00e2m ho\u1eb7c chai \u0111\u1ef1ng b\u1eb1ng nh\u1ef1a ho\u1eb7c th\u1ee7y tinh. Trung h\u00f2a ch\u1ea5t t\u00e1ch (n\u1ebfu c\u00f3 th\u1ec3) v\u00e0 \u0111\u1eb7t c\u00e1c t\u1ebf b\u00e0o v\u00e0o \u0111\u00e1. \u0110\u1ec3 thu h\u1ed3i th\u00eam c\u00e1c t\u1ebf b\u00e0o t\u00e1ch r\u1eddi c\u00f2n s\u00f3t l\u1ea1i trong bu\u1ed3ng, n\u00ean r\u1eeda th\u00eam m\u1ed9t ho\u1eb7c nhi\u1ec1u l\u1ea7n:<\/p>\n

a. \u0110\u1ec3 d\u1ec5 d\u00e0ng lo\u1ea1i b\u1ecf c\u00e1c t\u1ebf b\u00e0o, b\u01b0\u1edbc r\u1eeda n\u00e0y c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n b\u1eb1ng CMF-PBS ho\u1eb7c m\u00f4i tr\u01b0\u1eddng, sau \u0111\u00f3 \u0111\u01b0\u1ee3c th\u00eam v\u00e0o huy\u1ec1n ph\u00f9 t\u1ebf b\u00e0o t\u1eeb l\u1ea7n t\u00e1ch \u0111\u1ea7u ti\u00ean.<\/p>\n

    \n
  • N\u1ebfu th\u1ea5y m\u1ed9t s\u1ed1 l\u01b0\u1ee3ng l\u1edbn c\u00e1c t\u1ebf b\u00e0o s\u1ed1ng trong dung d\u1ecbch r\u1eeda, th\u00ec n\u00ean r\u1eeda l\u1ea7n th\u1ee9 hai ho\u1eb7c c\u00f3 th\u1ec3 c\u1ea7n \u0111i\u1ec1u ch\u1ec9nh dung d\u1ecbch t\u00e1ch ho\u1eb7c quy tr\u00ecnh thu ho\u1ea1ch. (Xem b\u01b0\u1edbc b b\u00ean d\u01b0\u1edbi.)<\/li>\n
  • N\u1ebfu dung d\u1ecbch r\u1eeda ch\u1ee9a \u00edt t\u1ebf b\u00e0o s\u1ed1ng, th\u00ec c\u00f3 th\u1ec3 b\u1ecf qua b\u01b0\u1edbc n\u00e0y.<\/li>\n<\/ul>\n

    b. \u0110\u1ed1i v\u1edbi c\u00e1c t\u1ebf b\u00e0o r\u1ea5t kh\u00f3 lo\u1ea1i b\u1ecf, n\u00ean r\u1eeda b\u1eb1ng dung d\u1ecbch t\u00e1ch \u0111\u01b0\u1ee3c l\u00e0m \u1ea5m tr\u01b0\u1edbc. C\u00f3 th\u1ec3 c\u1ea7n \u1ee7 t\u1ebf b\u00e0o trong v\u00e0i ph\u00fat \u0111\u1ec3 dung d\u1ecbch c\u00f3 th\u1eddi gian t\u00e1c \u0111\u1ed9ng l\u00ean c\u00e1c t\u1ebf b\u00e0o c\u00f2n b\u00e1m d\u00ednh.<\/p>\n

      \n
    • N\u1ebfu th\u1ea5y m\u1ed9t s\u1ed1 l\u01b0\u1ee3ng l\u1edbn t\u1ebf b\u00e0o s\u1ed1ng trong l\u1ea7n thu ho\u1ea1ch th\u1ee9 hai, th\u00ec c\u00f3 th\u1ec3 c\u1ea7n \u0111i\u1ec1u ch\u1ec9nh dung d\u1ecbch t\u00e1ch ho\u1eb7c quy tr\u00ecnh thu ho\u1ea1ch ban \u0111\u1ea7u.<\/li>\n
    • N\u1ebfu l\u1ea7n thu ho\u1ea1ch th\u1ee9 hai ch\u1ee9a \u00edt t\u1ebf b\u00e0o s\u1ed1ng, th\u00ec c\u00f3 th\u1ec3 b\u1ecf qua b\u01b0\u1edbc n\u00e0y.<\/li>\n<\/ul>\n

      6. V\u00f4 hi\u1ec7u h\u00f3a ho\u1eb7c lo\u1ea1i b\u1ecf c\u00e1c t\u00e1c nh\u00e2n ph\u00e2n ly.<\/p>\n

        \n
      • M\u1ed9t s\u1ed1 t\u00e1c nh\u00e2n ph\u00e2n ly n\u00ean \u0111\u01b0\u1ee3c lo\u1ea1i b\u1ecf ngay l\u1eadp t\u1ee9c b\u1eb1ng c\u00e1ch ly t\u00e2m \u0111\u1ec3 ng\u0103n ng\u1eeba s\u1ef1 k\u00e9o theo c\u00f3 th\u1ec3 g\u00e2y ra t\u00ecnh tr\u1ea1ng b\u00e1m d\u00ednh t\u1ebf b\u00e0o k\u00e9m ho\u1eb7c \u0111\u1ed9c t\u00ednh, \u0111\u1eb7c bi\u1ec7t l\u00e0 khi k\u1ebft h\u1ee3p v\u1edbi m\u00f4i tr\u01b0\u1eddng kh\u00f4ng c\u00f3 huy\u1ebft thanh.<\/li>\n
      • Tuy nhi\u00ean, c\u00e1c t\u00e1c nh\u00e2n ph\u00e2n ly kh\u00e1c, ch\u1eb3ng h\u1ea1n nh\u01b0 trypsin, c\u00f3 th\u1ec3 b\u1ecb v\u00f4 hi\u1ec7u h\u00f3a b\u1eb1ng c\u00e1ch th\u00eam m\u00f4i tr\u01b0\u1eddng l\u1ea1nh c\u00f3 huy\u1ebft thanh ho\u1eb7c ch\u1ea5t \u1ee9c ch\u1ebf trypsin v\u00e0 kh\u00f4ng c\u1ea7n ph\u1ea3i lo\u1ea1i b\u1ecf.<\/li>\n
      • N\u1ebfu mu\u1ed1n lo\u1ea1i b\u1ecf, h\u00e3y ly t\u00e2m h\u1ed7n d\u1ecbch t\u1ebf b\u00e0o \u1edf t\u1ed1c \u0111\u1ed9 100 x g trong 5 ph\u00fat. Sau \u0111\u00f3, lo\u1ea1i b\u1ecf m\u00f4i tr\u01b0\u1eddng ch\u1ee9a t\u00e1c nh\u00e2n ph\u00e2n ly v\u00e0 thay th\u1ebf b\u1eb1ng m\u00f4i tr\u01b0\u1eddng m\u1edbi.<\/li>\n<\/ul>\n

        7. \u0110\u1ebfm c\u00e1c t\u1ebf b\u00e0o \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh s\u1ea3n l\u01b0\u1ee3ng v\u00e0 kh\u1ea3 n\u0103ng s\u1ed1ng c\u1ee7a t\u1ebf b\u00e0o.<\/p>\n","protected":false},"featured_media":5923,"parent":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-4425","service","type-service","status-publish","has-post-thumbnail","hentry","service-category-quy-trinh-huong-dan-su-dung"],"acf":[],"yoast_head":"\nT\u1ebf B\u00e0o Trong B\u00ecnh Nu\u00f4i C\u1ea5y T\u1ebf B\u00e0o Nhi\u1ec1u T\u1ea7ng \u0110\u01b0\u1ee3c Thu Ho\u1ea1ch Nh\u01b0 Th\u1ebf N\u00e0o?<\/title>\n<meta name=\"description\" content=\"C\u00e1c b\u01b0\u1edbc \u0111\u1ec3 thu ho\u1ea1ch t\u1ebf b\u00e0o trong b\u00ecnh nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o nhi\u1ec1u t\u1ea7ng\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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