{"id":4429,"date":"2025-01-08T09:58:54","date_gmt":"2025-01-08T09:58:54","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/service\/frequently-asked-questions-on-cd138-cell-isolation-for-increased-fish-sensitivity\/"},"modified":"2025-09-29T13:07:36","modified_gmt":"2025-09-29T06:07:36","slug":"cac-cau-hoi-thuong-gap-ve-phan-lap-te-bao-cd138-de-tang-do-nhay-trong-thu-nghiem-fish","status":"publish","type":"service","link":"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cac-cau-hoi-thuong-gap-ve-phan-lap-te-bao-cd138-de-tang-do-nhay-trong-thu-nghiem-fish\/","title":{"rendered":"C\u00e1c C\u00e2u H\u1ecfi Th\u01b0\u1eddng G\u1eb7p V\u1ec1 Ph\u00e2n L\u1eadp T\u1ebf B\u00e0o CD138+ \u0110\u1ec3 T\u0103ng \u0110\u1ed9 Nh\u1ea1y Trong Th\u1eed Nghi\u1ec7m FISH"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cac-cau-hoi-thuong-gap-ve-phan-lap-te-bao-cd138-de-tang-do-nhay-trong-thu-nghiem-fish\/#1_Lam_the_nao_de_danh_gia_do_tinh_khiet_cua_te_bao_plasma_CD138_sau_khi_phan_lap\" >1. L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 tinh khi\u1ebft c\u1ee7a t\u1ebf b\u00e0o plasma CD138+ sau khi ph\u00e2n l\u1eadp?&nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cac-cau-hoi-thuong-gap-ve-phan-lap-te-bao-cd138-de-tang-do-nhay-trong-thu-nghiem-fish\/#2_Lam_cach_nao_toi_co_the_xac_dinh_kha_nang_thu_hoi_cua_cac_te_bao_plasma_CD138_sau_khi_phan_lap_bang_Bo_xet_nghiem_duong_tinh_EasySep%E2%84%A2_Human_Whole_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II\" >2. L\u00e0m c\u00e1ch n\u00e0o t\u00f4i c\u00f3 th\u1ec3 x\u00e1c \u0111\u1ecbnh kh\u1ea3 n\u0103ng thu h\u1ed3i c\u1ee7a c\u00e1c t\u1ebf b\u00e0o plasma CD138+ sau khi ph\u00e2n l\u1eadp b\u1eb1ng B\u1ed9 x\u00e9t nghi\u1ec7m d\u01b0\u01a1ng t\u00ednh EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II?&nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cac-cau-hoi-thuong-gap-ve-phan-lap-te-bao-cd138-de-tang-do-nhay-trong-thu-nghiem-fish\/#3_Su_khac_biet_giua_EasySep%E2%84%A2_Human_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II_17887_va_EasySep%E2%84%A2_Human_CD138_Positive_Selection_Kit_II_17877_la_gi\" >3. S\u1ef1 kh\u00e1c bi\u1ec7t gi\u1eefa EasySep\u2122 Human Blood and Bone Marrow CD138 Positive Selection Kit II (#17887) v\u00e0 EasySep\u2122 Human CD138 Positive Selection Kit II (#17877) l\u00e0 g\u00ec?&nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cac-cau-hoi-thuong-gap-ve-phan-lap-te-bao-cd138-de-tang-do-nhay-trong-thu-nghiem-fish\/#4_Chung_ta_co_the_phan_lap_te_bao_plasma_CD138_tu_cac_mau_tuy_xuong_duoc_bao_quan_lanh_truoc_do_bang_bo_EasySep%E2%84%A2_Human_Whole_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II_17887_duoc_khong\" >4. Ch\u00fang ta c\u00f3 th\u1ec3 ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma CD138+ t\u1eeb c\u00e1c m\u1eabu t\u1ee7y x\u01b0\u01a1ng \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n l\u1ea1nh tr\u01b0\u1edbc \u0111\u00f3 b\u1eb1ng b\u1ed9 EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (#17887) \u0111\u01b0\u1ee3c kh\u00f4ng?&nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cac-cau-hoi-thuong-gap-ve-phan-lap-te-bao-cd138-de-tang-do-nhay-trong-thu-nghiem-fish\/#5_Viec_phan_lap_te_bao_plasma_CD138_bang_san_pham_EasySep%E2%84%A2_Human_Whole_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II_co_tuong_thich_voi_cac_ung_dung_tiep_theo_nhu_tach_chiet_RNADNA_giai_trinh_tu_the_he_moi_next-generation_sequencing_NGS_va_te_bao_hoc_dong_chay_khong\" >5. Vi\u1ec7c ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma CD138+ b\u1eb1ng s\u1ea3n ph\u1ea9m EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II c\u00f3 t\u01b0\u01a1ng th\u00edch v\u1edbi c\u00e1c \u1ee9ng d\u1ee5ng ti\u1ebfp theo nh\u01b0 t\u00e1ch chi\u1ebft RNA\/DNA, gi\u1ea3i tr\u00ecnh t\u1ef1 th\u1ebf h\u1ec7 m\u1edbi (next-generation sequencing NGS) v\u00e0 t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y kh\u00f4ng?&nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cac-cau-hoi-thuong-gap-ve-phan-lap-te-bao-cd138-de-tang-do-nhay-trong-thu-nghiem-fish\/#6_Co_the_loai_bo_hat_tu_tren_be_mat_te_bao_sau_khi_phan_lap_khong\" >6. C\u00f3 th\u1ec3 lo\u1ea1i b\u1ecf h\u1ea1t t\u1eeb tr\u00ean b\u1ec1 m\u1eb7t t\u1ebf b\u00e0o sau khi ph\u00e2n l\u1eadp kh\u00f4ng?&nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cac-cau-hoi-thuong-gap-ve-phan-lap-te-bao-cd138-de-tang-do-nhay-trong-thu-nghiem-fish\/#7_Neu_mau_mau_duoc_su_dung_thay_cho_mau_tuy_xuong_de_phan_lap_te_bao_plasma_CD138_bang_EasySep%E2%84%A2_Human_Whole_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II_thi_mau_nay_nen_duoc_xu_ly_nhu_the_nao\" >7. N\u1ebfu m\u1eabu m\u00e1u \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng thay cho m\u1eabu t\u1ee7y x\u01b0\u01a1ng \u0111\u1ec3 ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma CD138+ b\u1eb1ng EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II, th\u00ec m\u1eabu n\u00e0y n\u00ean \u0111\u01b0\u1ee3c x\u1eed l\u00fd nh\u01b0 th\u1ebf n\u00e0o?&nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/cac-cau-hoi-thuong-gap-ve-phan-lap-te-bao-cd138-de-tang-do-nhay-trong-thu-nghiem-fish\/#8_Khi_thuc_hien_phan_lap_te_bao_plasma_CD138_tu_dong_bang_EasySep%E2%84%A2_Human_Whole_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II_tren_thiet_bi_RoboSepTM-S_thi_co_the_su_dung_may_quet_ma_vach_cam_tay_de_quet_ma_vach_ID_mau_khong\" >8. Khi th\u1ef1c hi\u1ec7n ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma CD138+ t\u1ef1 \u0111\u1ed9ng b\u1eb1ng EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II tr\u00ean thi\u1ebft b\u1ecb RoboSepTM-S th\u00ec c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng m\u00e1y qu\u00e9t m\u00e3 v\u1ea1ch c\u1ea7m tay \u0111\u1ec3 qu\u00e9t m\u00e3 v\u1ea1ch ID m\u1eabu kh\u00f4ng?&nbsp;<\/a><\/li><\/ul><\/nav><\/div>\n<h3 style=\"text-align:justify\"><span class=\"ez-toc-section\" id=\"1_Lam_the_nao_de_danh_gia_do_tinh_khiet_cua_te_bao_plasma_CD138_sau_khi_phan_lap\"><\/span>1. L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 tinh khi\u1ebft c\u1ee7a t\u1ebf b\u00e0o plasma CD138+ sau khi ph\u00e2n l\u1eadp?&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p style=\"text-align:justify\">Ph\u01b0\u01a1ng ph\u00e1p t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u00ec c\u00f3 \u0111\u1ed9 ch\u00ednh x\u00e1c t\u1ed1i \u01b0u. S\u1ea3n ph\u1ea9m kh\u00e1ng th\u1ec3 kh\u00e1ng CD138 (Syndecan-1) c\u1ee7a ng\u01b0\u1eddi, d\u00f2ng MI15 (Catalog #60003) c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho m\u1ee5c \u0111\u00edch n\u00e0y. Tuy nhi\u00ean, vi\u1ec7c chu\u1ea9n \u0111\u1ed9 kh\u00e1ng th\u1ec3 s\u1ebd r\u1ea5t quan tr\u1ecdng khi \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 tinh khi\u1ebft c\u1ee7a m\u1eabu sau khi ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma CD138+. N\u1ed3ng \u0111\u1ed9 kh\u00e1ng th\u1ec3 s\u1ebd c\u1ea7n \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh cho \u0111\u1ebfn khi t\u00edn hi\u1ec7u kh\u00e1ng th\u1ec3 hu\u1ef3nh quang \u0111\u1ea1t m\u1ee9c \u1ed5n \u0111\u1ecbnh trong qu\u00e1 tr\u00ecnh \u0111\u00e1nh gi\u00e1 t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y.&nbsp;<\/p>\n<p style=\"text-align:justify\">Ph\u01b0\u01a1ng ph\u00e1p thay th\u1ebf \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 tinh khi\u1ebft th\u00f4ng qua ph\u01b0\u01a1ng ph\u00e1p t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y?&nbsp;<\/p>\n<p style=\"text-align:justify\">+ Vi\u1ec7c ph\u00e1t hi\u1ec7n c\u00e1c t\u00edn hi\u1ec7u n\u1ed9i b\u00e0 nh\u01b0 chu\u1ed7i nh\u1eb9 kappa v\u00e0 lambda b\u1eb1ng kh\u00e1ng th\u1ec3 \u0111\u01a1n d\u00f2ng anti-human \u0111\u00e3 li\u00ean h\u1ee3p v\u1edbi hu\u1ef3nh quang (fluorchrome) t\u01b0\u01a1ng \u1ee9ng.&nbsp;<\/p>\n<p style=\"text-align:justify\">+ S\u1eed d\u1ee5ng kh\u00e1ng th\u1ec3 li\u00ean h\u1ee3p hu\u1ef3nh quang: <a href=\"https:\/\/www.stemcell.com\/anti-human-cd38-antibody-clone-hit2.html\">Anti-Human CD38 Antibody, Clone HIT2<\/a>\u202f(Catalog #60014) v\u00e0 <a href=\"https:\/\/www.stemcell.com\/anti-human-cd45-antibody-clone-hi30.html\">Anti-Human CD45 Antibody, Clone HI30<\/a>\u202f(Catalog #60018) \u0111\u1ec3 ph\u00e1t hi\u1ec7n t\u1ebf b\u00e0o CD38+CD45variable .&nbsp;<\/p>\n<p style=\"text-align:justify\">+ S\u1eed d\u1ee5ng kh\u00e1ng th\u1ec3 th\u1ee9 c\u1ea5p li\u00ean h\u1ee3p hu\u1ef3nh quang, nh\u01b0 l\u00e0 <a href=\"https:\/\/www.stemcell.com\/goat-anti-mouse-igg-h-l-antibody-polyclonal.html\">Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal<\/a>\u202f(Catalog #60138).&nbsp;<\/p>\n<p style=\"text-align:justify\">\u0110\u1ec3 bi\u1ebft c\u00e1c khuy\u1ebfn ngh\u1ecb v\u1ec1 c\u00e1ch bao g\u1ed3m c\u00e1c bi\u1ec7n ph\u00e1p ki\u1ec3m so\u00e1t th\u00edch h\u1ee3p cho ph\u00e9p \u0111o t\u1ebf b\u00e0o theo d\u00f2ng ch\u1ea3y v\u00e0 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p th\u1ef1c h\u00e0nh t\u1ed1t nh\u1ea5t \u0111\u1ec3 ch\u1ecdn v\u00f9ng (gating), vui l\u00f2ng xem: \u202f<a href=\"https:\/\/www.stemcell.com\/technical-resources\/educational-materials\/tech-tips\/considerations-for-facs-gating.html\">Tech Tip: Considerations for Flow Cytometry Gating<\/a>.&nbsp;<\/p>\n<h3 style=\"text-align:justify\"><span class=\"ez-toc-section\" id=\"2_Lam_cach_nao_toi_co_the_xac_dinh_kha_nang_thu_hoi_cua_cac_te_bao_plasma_CD138_sau_khi_phan_lap_bang_Bo_xet_nghiem_duong_tinh_EasySep%E2%84%A2_Human_Whole_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II\"><\/span>2. L\u00e0m c\u00e1ch n\u00e0o t\u00f4i c\u00f3 th\u1ec3 x\u00e1c \u0111\u1ecbnh kh\u1ea3 n\u0103ng thu h\u1ed3i c\u1ee7a c\u00e1c t\u1ebf b\u00e0o plasma CD138+ sau khi ph\u00e2n l\u1eadp b\u1eb1ng B\u1ed9 x\u00e9t nghi\u1ec7m d\u01b0\u01a1ng t\u00ednh EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II?&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p style=\"text-align:justify\">Vi\u1ec7c x\u00e1c \u0111\u1ecbnh m\u1ee9c thu h\u1ed3i t\u1ebf b\u00e0o plasma CD138+ l\u00e0 th\u01b0\u1edbc \u0111o ch\u00ednh x\u00e1c h\u01a1n v\u1ec1 hi\u1ec7u su\u1ea5t c\u1ee7a qu\u00e1 tr\u00ecnh ph\u00e2n l\u1eadp so v\u1edbi vi\u1ec7c ch\u1ec9 t\u00ednh s\u1ea3n l\u01b0\u1ee3ng cu\u1ed1i c\u00f9ng c\u1ee7a c\u00e1c t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp. Vi\u1ec7c t\u00ednh \u0111\u1ed9 thu h\u1ed3i s\u1ebd t\u00ednh \u0111\u1ebfn s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o plasma CD138+ th\u1ef1c t\u1ebf trong m\u1eabu ban \u0111\u1ea7u, trong khi ch\u1ec9 x\u00e1c \u0111\u1ecbnh s\u1ea3n l\u01b0\u1ee3ng cu\u1ed1i c\u00f9ng c\u1ee7a c\u00e1c t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp th\u00ec kh\u00f4ng c\u00f3.&nbsp;<\/p>\n<p style=\"text-align:justify\">\u0110\u1ec3 x\u00e1c \u0111\u1ecbnh t\u1ef7 l\u1ec7 ph\u1ea7n tr\u0103m t\u1ebf b\u00e0o plasma CD138+ \u0111\u00e3 \u0111\u01b0\u1ee3c thu h\u1ed3i t\u1eeb m\u1eabu ban \u0111\u1ea7u sau khi ph\u00e2n l\u1eadp b\u1eb1ng b\u1ed9 s\u1ea3n ph\u1ea9m, vui l\u00f2ng xem <a href=\"https:\/\/www.stemcell.com\/technical-resources\/educational-materials\/tech-tips\/assessment-of-your-starting-sample-and-calculation-of-recovery-from-cell-isolation-procedures.html\">Tech Tip: Calculation of Recovery from Cell Isolation Procedures<\/a>.&nbsp;<\/p>\n<h3 style=\"text-align:justify\"><span class=\"ez-toc-section\" id=\"3_Su_khac_biet_giua_EasySep%E2%84%A2_Human_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II_17887_va_EasySep%E2%84%A2_Human_CD138_Positive_Selection_Kit_II_17877_la_gi\"><\/span>3. S\u1ef1 kh\u00e1c bi\u1ec7t gi\u1eefa EasySep\u2122 Human Blood and Bone Marrow CD138 Positive Selection Kit II (#17887) v\u00e0 EasySep\u2122 Human CD138 Positive Selection Kit II (#17877) l\u00e0 g\u00ec?&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p style=\"text-align:justify\">B\u1ed9 kit EasySep\u2122 Human CD138 Positive Selection Kit II (#17877) \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n \u0111\u1ec3 ph\u00e2n l\u1eadp CD138+ t\u1eeb c\u00e1c t\u1ebf b\u00e0o \u0111\u01a1n nh\u00e2n \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb t\u1ee7y x\u01b0\u01a1ng ho\u1eb7c m\u1eabu m\u00e1u th\u00f4ng qua ph\u01b0\u01a1ng ph\u00e1p ly t\u00e2m gradient t\u1ec9 tr\u1ecdng.&nbsp;&nbsp;<\/p>\n<p style=\"text-align:justify\">B\u1ed9 kit EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (#17887) \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n \u0111\u1ec3 ph\u00e2n l\u1eadp CD138+ tr\u1ef1c ti\u1ebfp t\u1eeb t\u1ee7y x\u01b0\u01a1ng ho\u1eb7c m\u1eabu m\u00e1u m\u00e0 kh\u00f4ng c\u1ea7n th\u00f4ng qua vi\u1ec7c ph\u00e2n l\u1eadp c\u00e1c t\u1ebf b\u00e0o \u0111\u01a1n nh\u00e2n.&nbsp;<\/p>\n<h3 style=\"text-align:justify\"><span class=\"ez-toc-section\" id=\"4_Chung_ta_co_the_phan_lap_te_bao_plasma_CD138_tu_cac_mau_tuy_xuong_duoc_bao_quan_lanh_truoc_do_bang_bo_EasySep%E2%84%A2_Human_Whole_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II_17887_duoc_khong\"><\/span>4. Ch\u00fang ta c\u00f3 th\u1ec3 ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma CD138+ t\u1eeb c\u00e1c m\u1eabu t\u1ee7y x\u01b0\u01a1ng \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n l\u1ea1nh tr\u01b0\u1edbc \u0111\u00f3 b\u1eb1ng b\u1ed9 EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (#17887) \u0111\u01b0\u1ee3c kh\u00f4ng?&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p style=\"text-align:justify\">Ch\u00fang t\u00f4i kh\u00f4ng khuy\u1ebfn ngh\u1ecb b\u1ea3o qu\u1ea3n l\u1ea1nh c\u00e1c m\u1eabu t\u1ee7y x\u01b0\u01a1ng v\u00ec bi\u1ec3u hi\u1ec7n protein CD138 tr\u00ean b\u1ec1 m\u1eb7t t\u1ebf b\u00e0o plasma s\u1ebd gi\u1ea3m \u0111\u00e1ng k\u1ec3 do b\u1ea3o qu\u1ea3n l\u1ea1nh so v\u1edbi c\u00e1c m\u1eabu t\u01b0\u01a1i. \u0110i\u1ec1u n\u00e0y s\u1ebd \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn hi\u1ec7u su\u1ea5t c\u1ee7a s\u1ef1 ph\u00e2n l\u1eadp. Vi\u1ec7c r\u00e3 \u0111\u00f4ng m\u1eabu c\u0169ng s\u1ebd \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn s\u1ef1 ph\u00e2n gi\u1ea3i \u0111\u00e1ng k\u1ec3 c\u00e1c t\u1ebf b\u00e0o h\u1ed3ng c\u1ea7u v\u00e0 b\u1ea1ch c\u1ea7u h\u1ea1t, \u0111i\u1ec1u n\u00e0y s\u1ebd \u1ea3nh h\u01b0\u1edfng h\u01a1n n\u1eefa \u0111\u1ebfn ch\u1ea5t l\u01b0\u1ee3ng m\u1eabu v\u00e0 s\u1ef1 ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma sau n\u00e0y. Thay v\u00e0o \u0111\u00f3, ch\u00fang t\u00f4i khuy\u00ean b\u1ea1n n\u00ean b\u1ea3o qu\u1ea3n m\u1eabu t\u1ee7y x\u01b0\u01a1ng \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng kh\u00f4ng qu\u00e1 72 gi\u1edd.&nbsp;<\/p>\n<p style=\"text-align:justify\">N\u1ebfu kh\u00f4ng th\u1ec3 tr\u00e1nh kh\u1ecfi vi\u1ec7c b\u1ea3o qu\u1ea3n l\u1ea1nh c\u00e1c m\u1eabu t\u1ee7y x\u01b0\u01a1ng, th\u00ec c\u00f3 1 khuy\u1ebfn ngh\u1ecb l\u00e0 n\u00ean \u1ee7 c\u00e1c t\u1ebf b\u00e0o \u0111\u00e3 r\u00e3 \u0111\u00f4ng b\u1eb1ng DNase I. Sau \u0111\u00f3, c\u00e1c t\u1ebf b\u00e0o plasma CD138+ c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng b\u1ed9 EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II. \u0110\u1ed9 tinh s\u1ea1ch v\u00e0 \u0111\u1ed9 thu h\u1ed3i c\u00f3 th\u1ec3 kh\u00f4ng b\u1eb1ng so v\u1edbi khi ph\u00e2n l\u1eadp b\u1eb1ng m\u1eabu t\u01b0\u01a1i b\u1edfi v\u00ec c\u00e1c l\u00fd do \u0111\u00e3 n\u00eau tr\u00ean. \u1ec3 bi\u1ebft c\u00e1c g\u1ee3i \u00fd v\u1ec1 c\u00e1ch r\u00e3 \u0111\u00f4ng c\u00e1c t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n l\u1ea1nh, vui l\u00f2ng xem quy tr\u00ecnh: <a href=\"https:\/\/www.stemcell.com\/technical-resources\/how-to-thaw-frozen-human-primary-cells.html\">How to Thaw Frozen Human Primary Cells<\/a>.&nbsp;<\/p>\n<p style=\"text-align:justify\">Ngo\u00e0i ra, tr\u01b0\u1edbc ti\u00ean, c\u00e1c t\u1ebf b\u00e0o \u0111\u01a1n nh\u00e2n c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb c\u00e1c m\u1eabu t\u1ee7y x\u01b0\u01a1ng \u0111\u00e3 r\u00e3 \u0111\u00f4ng th\u00f4ng qua ph\u01b0\u01a1ng ph\u00e1p ly t\u00e2m gradient m\u1eadt \u0111\u1ed9, sau \u0111\u00f3 l\u00e0 ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma CD138+ b\u1eb1ng b\u1ed9 EasySep\u2122 Human CD138 Positive Selection Kit II (#17877). B\u01b0\u1edbc ph\u00e2n l\u1eadp gradient m\u1eadt \u0111\u1ed9 c\u00f3 kh\u1ea3 n\u0103ng l\u00e0m gi\u1ea3m s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a c\u00e1c t\u1ebf b\u00e0o ch\u1ebft v\u00e0 m\u1ea3nh v\u1ee5n trong m\u1eabu sau r\u00e3 \u0111\u00f4ng, \u0111i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng x\u1ea5u \u0111\u1ebfn qu\u00e1 tr\u00ecnh ph\u00e2n l\u1eadp t\u1ebf b\u00e0o v\u00e0 \u0111\u1ed9 tinh khi\u1ebft c\u1ee7a CD138+.&nbsp;<\/p>\n<h3 style=\"text-align:justify\"><span class=\"ez-toc-section\" id=\"5_Viec_phan_lap_te_bao_plasma_CD138_bang_san_pham_EasySep%E2%84%A2_Human_Whole_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II_co_tuong_thich_voi_cac_ung_dung_tiep_theo_nhu_tach_chiet_RNADNA_giai_trinh_tu_the_he_moi_next-generation_sequencing_NGS_va_te_bao_hoc_dong_chay_khong\"><\/span>5. Vi\u1ec7c ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma CD138+ b\u1eb1ng s\u1ea3n ph\u1ea9m EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II c\u00f3 t\u01b0\u01a1ng th\u00edch v\u1edbi c\u00e1c \u1ee9ng d\u1ee5ng ti\u1ebfp theo nh\u01b0 t\u00e1ch chi\u1ebft RNA\/DNA, gi\u1ea3i tr\u00ecnh t\u1ef1 th\u1ebf h\u1ec7 m\u1edbi (next-generation sequencing NGS) v\u00e0 t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y kh\u00f4ng?&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p style=\"text-align:justify\">C\u00f3, DNA\/RNA c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c t\u00e1ch chi\u1ebft t\u1eeb t\u1ebf b\u00e0o plasma sau khi ph\u00e2n l\u1eadp v\u1edbi b\u1ed9 kit n\u00e0y. T\u1ebf b\u00e0o plasma c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch s\u00e2u h\u01a1n b\u1eb1ng NGS v\u00e0 ph\u01b0\u01a1ng ph\u00e1p t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y.&nbsp;<\/p>\n<p style=\"text-align:justify\">\u0110\u1ec3 lo\u1ea1i b\u1ecf h\u1ea1t t\u1eeb (EasySep\u2122 Dextran RapidSpheres\u2122 50100) trong \u1ee9ng d\u1ee5ng t\u00e1ch chi\u1ebft DNA\/RNA, vui l\u00f2ng xem <a href=\"https:\/\/www.stemcell.com\/technical-resources\/educational-materials\/tech-tips\/acquiring-dna-or-rna-from-positively-selected-cells.html\">Tech Tip: Acquiring DNA or RNA from Positively Selected Cells<\/a>.&nbsp;&nbsp;<\/p>\n<p style=\"text-align:justify\">\u0110\u1ec3 bi\u1ebft th\u00eam chi ti\u1ebft v\u1ec1 kh\u1ea3 n\u0103ng t\u01b0\u01a1ng th\u00edch c\u1ee7a b\u1ed9 kit v\u1edbi c\u00e1c \u1ee9ng d\u1ee5ng ti\u1ebfp theo, vui l\u00f2ng xem <a href=\"https:\/\/www.stemcell.com\/easysep-cell-separation-data\">EasySep\u2122 Performance Data<\/a>.&nbsp;<\/p>\n<h3 style=\"text-align:justify\"><span class=\"ez-toc-section\" id=\"6_Co_the_loai_bo_hat_tu_tren_be_mat_te_bao_sau_khi_phan_lap_khong\"><\/span>6. C\u00f3 th\u1ec3 lo\u1ea1i b\u1ecf h\u1ea1t t\u1eeb tr\u00ean b\u1ec1 m\u1eb7t t\u1ebf b\u00e0o sau khi ph\u00e2n l\u1eadp kh\u00f4ng?&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p style=\"text-align:justify\">Kh\u00f4ng, c\u00e1c h\u1ea1t t\u1eeb t\u00ednh kh\u00f4ng th\u1ec3 gi\u1ea3i ph\u00f3ng kh\u1ecfi b\u1ec1 m\u1eb7t t\u1ebf b\u00e0o sau khi ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma v\u00e0 s\u1ebd t\u1ed3n t\u1ea1i tr\u00ean b\u1ec1 m\u1eb7t t\u1ebf b\u00e0o \u0111\u00e3 ph\u00e2n l\u1eadp. Tuy nhi\u00ean, s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a c\u00e1c h\u1ea1t t\u1eeb t\u00ednh tr\u00ean b\u1ec1 m\u1eb7t t\u1ebf b\u00e0o c\u1ee7a t\u1ebf b\u00e0o plasma CD138+ s\u1ebd kh\u00f4ng \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn h\u1ea7u h\u1ebft c\u00e1c \u1ee9ng d\u1ee5ng ph\u1ed5 bi\u1ebfn ti\u1ebfp theo, nh\u01b0 \u0111\u01b0\u1ee3c th\u1ec3 hi\u1ec7n trong <a href=\"https:\/\/www.stemcell.com\/easysep-cell-separation-data\">d\u1eef li\u1ec7u<\/a> c\u1ee7a c\u00e1c b\u1ed9 EasySep\u2122 kh\u00e1c.&nbsp;<\/p>\n<h3 style=\"text-align:justify\"><span class=\"ez-toc-section\" id=\"7_Neu_mau_mau_duoc_su_dung_thay_cho_mau_tuy_xuong_de_phan_lap_te_bao_plasma_CD138_bang_EasySep%E2%84%A2_Human_Whole_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II_thi_mau_nay_nen_duoc_xu_ly_nhu_the_nao\"><\/span>7. N\u1ebfu m\u1eabu m\u00e1u \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng thay cho m\u1eabu t\u1ee7y x\u01b0\u01a1ng \u0111\u1ec3 ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma CD138+ b\u1eb1ng EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II, th\u00ec m\u1eabu n\u00e0y n\u00ean \u0111\u01b0\u1ee3c x\u1eed l\u00fd nh\u01b0 th\u1ebf n\u00e0o?&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p style=\"text-align:justify\">Kh\u00f4ng c\u1ea7n x\u1eed l\u00fd m\u1eabu khi b\u1eaft \u0111\u1ea7u v\u1edbi m\u1eabu m\u00e1u. Mi\u1ec5n l\u00e0 m\u00e1u \u0111\u01b0\u1ee3c thu th\u1eadp trong \u1ed1ng ch\u1ee9a ch\u1ea5t ch\u1ed1ng \u0111\u00f4ng m\u00e1u, ng\u01b0\u1eddi ta c\u00f3 th\u1ec3 ti\u1ebfn h\u00e0nh quy tr\u00ecnh ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma CD138+ th\u1ee7 c\u00f4ng ho\u1eb7c t\u1ef1 \u0111\u1ed9ng nh\u01b0 \u0111\u01b0\u1ee3c m\u00f4 t\u1ea3 trong t\u1edd th\u00f4ng tin s\u1ea3n ph\u1ea9m c\u1ee7a b\u1ed9 s\u1ea3n ph\u1ea9m.&nbsp;<\/p>\n<h3 style=\"text-align:justify\"><span class=\"ez-toc-section\" id=\"8_Khi_thuc_hien_phan_lap_te_bao_plasma_CD138_tu_dong_bang_EasySep%E2%84%A2_Human_Whole_Blood_and_Bone_Marrow_CD138_Positive_Selection_Kit_II_tren_thiet_bi_RoboSepTM-S_thi_co_the_su_dung_may_quet_ma_vach_cam_tay_de_quet_ma_vach_ID_mau_khong\"><\/span>8. Khi th\u1ef1c hi\u1ec7n ph\u00e2n l\u1eadp t\u1ebf b\u00e0o plasma CD138+ t\u1ef1 \u0111\u1ed9ng b\u1eb1ng EasySep\u2122 Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II tr\u00ean thi\u1ebft b\u1ecb RoboSepTM-S th\u00ec c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng m\u00e1y qu\u00e9t m\u00e3 v\u1ea1ch c\u1ea7m tay \u0111\u1ec3 qu\u00e9t m\u00e3 v\u1ea1ch ID m\u1eabu kh\u00f4ng?&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p style=\"text-align:justify\">C\u00f3, m\u00e1y qu\u00e9t m\u00e3 v\u1ea1ch c\u1ea7m tay b\u00ean ngo\u00e0i c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c k\u1ebft n\u1ed1i v\u1edbi thi\u1ebft b\u1ecb th\u00f4ng qua m\u1ed9t trong c\u00e1c c\u1ed5ng USB \u1edf m\u1eb7t tr\u01b0\u1edbc. \u0110i\u1ec1u n\u00e0y s\u1ebd cho ph\u00e9p ng\u01b0\u1eddi d\u00f9ng qu\u00e9t m\u00e3 v\u1ea1ch ID m\u1eabu c\u0169ng nh\u01b0 danh m\u1ee5c v\u00e0 s\u1ed1 l\u00f4 c\u1ee7a chai Dung d\u1ecbch \u0111\u1ec7m RoboSep\u2122 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong qu\u00e1 tr\u00ecnh ph\u00e2n l\u1eadp (thay v\u00ec th\u00eam th\u00f4ng tin n\u00e0y theo c\u00e1ch th\u1ee7 c\u00f4ng trong qu\u00e1 tr\u00ecnh thi\u1ebft l\u1eadp). Danh m\u1ee5c v\u00e0 s\u1ed1 l\u00f4 c\u1ee7a l\u1ecd thu\u1ed1c th\u1eed trong b\u1ed9 s\u1ea3n ph\u1ea9m s\u1ebd \u0111\u01b0\u1ee3c ghi l\u1ea1i t\u1ef1 \u0111\u1ed9ng th\u00f4ng qua m\u00e1y qu\u00e9t m\u00e3 v\u1ea1ch t\u00edch h\u1ee3p. Qu\u00e9t b\u1eb1ng m\u00e1y qu\u00e9t t\u00edch h\u1ee3p c\u0169ng s\u1ebd cho ph\u00e9p x\u00e1c minh r\u1eb1ng thu\u1ed1c th\u1eed \u0111\u01b0\u1ee3c \u0111\u1eb7t v\u00e0o \u0111\u00fang khe tr\u00ean b\u0103ng chuy\u1ec1n thi\u1ebft b\u1ecb. M\u00e1y qu\u00e9t t\u00edch h\u1ee3p n\u00e0y c\u00f3 th\u1ec3 b\u1ecb t\u1eaft n\u1ebfu c\u1ea7n v\u00e0 c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng m\u00e1y qu\u00e9t c\u1ea7m tay \u0111\u1ec3 thay th\u1ebf.&nbsp;<\/p>\n<p style=\"text-align:justify\">Link g\u1ed1c b\u00e0i vi\u1ebft: <a href=\"https:\/\/www.stemcell.com\/technical-resources\/cell-isolation-for-fish-testing-faqs.html\">Frequently Asked Questions on CD138\u207a Cell Isolation for Increased FISH Sensitivity<\/a>&nbsp;<\/p>\n","protected":false},"featured_media":5930,"parent":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-4429","service","type-service","status-publish","has-post-thumbnail","hentry","service-category-quy-trinh-huong-dan-su-dung"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>C\u00e1c C\u00e2u H\u1ecfi Th\u01b0\u1eddng G\u1eb7p V\u1ec1 Ph\u00e2n L\u1eadp T\u1ebf B\u00e0o CD138+ \u0110\u1ec3 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