{"id":4439,"date":"2025-04-03T04:48:24","date_gmt":"2025-04-03T04:48:24","guid":{"rendered":"http:\/\/biogroupvietnam-worldpress.local\/service\/nk-cell-culture-and-differentiation-using-stemspan-nk-cell-generation-kit\/"},"modified":"2025-09-29T13:07:55","modified_gmt":"2025-09-29T06:07:55","slug":"quy-trinh-nuoi-cay-va-biet-hoa-te-bao-nk-su-dung-stemspan-nk-cell-generation-kit","status":"publish","type":"service","link":"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/quy-trinh-nuoi-cay-va-biet-hoa-te-bao-nk-su-dung-stemspan-nk-cell-generation-kit\/","title":{"rendered":"Quy Tr\u00ecnh Nu\u00f4i C\u1ea5y V\u00e0 Bi\u1ec7t H\u00f3a T\u1ebf B\u00e0o NK S\u1eed D\u1ee5ng StemSpan\u2122 NK Cell Generation Kit"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_80 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">M\u1ee5c l\u1ee5c<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/quy-trinh-nuoi-cay-va-biet-hoa-te-bao-nk-su-dung-stemspan-nk-cell-generation-kit\/#1_Gioi_thieu\" >1. Gi\u1edbi thi\u1ec7u&nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/quy-trinh-nuoi-cay-va-biet-hoa-te-bao-nk-su-dung-stemspan-nk-cell-generation-kit\/#2_Hoa_chat_vat_tu\" >2. H\u00f3a ch\u1ea5t, v\u1eadt t\u01b0&nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/quy-trinh-nuoi-cay-va-biet-hoa-te-bao-nk-su-dung-stemspan-nk-cell-generation-kit\/#3_Thiet_bi_dung_cu\" >3. Thi\u1ebft b\u1ecb, d\u1ee5ng c\u1ee5&nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/quy-trinh-nuoi-cay-va-biet-hoa-te-bao-nk-su-dung-stemspan-nk-cell-generation-kit\/#4_Biet_hoa_thanh_te_bao_NK\" >4. Bi\u1ec7t h\u00f3a th\u00e0nh t\u1ebf b\u00e0o NK&nbsp;<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/biogroupvietnam.com\/vi\/dich-vu\/quy-trinh-nuoi-cay-va-biet-hoa-te-bao-nk-su-dung-stemspan-nk-cell-generation-kit\/#5_Du_lieu\" >5. D\u1eef li\u1ec7u&nbsp;<\/a><\/li><\/ul><\/nav><\/div>\n<h4><span class=\"ez-toc-section\" id=\"1_Gioi_thieu\"><\/span><strong>1. Gi\u1edbi thi\u1ec7u<\/strong>&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h4>\n<p>T\u1ebf b\u00e0o gi\u1ebft t\u1ef1 nhi\u00ean (NK) l\u00e0 t\u1ebf b\u00e0o mi\u1ec5n d\u1ecbch b\u1ea9m sinh c\u00f3 kh\u1ea3 n\u0103ng ti\u00eau di\u1ec7t c\u00e1c t\u1ebf b\u00e0o b\u1ecb nhi\u1ec5m kh\u1ed1i u ho\u1eb7c vi-r\u00fat c\u0169ng nh\u01b0 ti\u1ebft ra c\u00e1c cytokine g\u00e2y vi\u00eam. T\u1ebf b\u00e0o NK c\u1ee7a ng\u01b0\u1eddi c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb t\u1ebf b\u00e0o g\u1ed1c t\u1ea1o m\u00e1u CD34+ v\u00e0 t\u1ebf b\u00e0o ti\u1ec1n th\u00e2n (HSPC) trong m\u00e1u d\u00e2y r\u1ed1n (CB) v\u00e0 t\u1ee7y x\u01b0\u01a1ng (BM). Nu\u00f4i c\u1ea5y trong \u1ed1ng nghi\u1ec7m \u0111\u1ec3 th\u00fac \u0111\u1ea9y s\u1ef1 bi\u1ec7t h\u00f3a c\u1ee7a t\u1ebf b\u00e0o CD34+ HSPC th\u00e0nh t\u1ebf b\u00e0o NK l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 h\u1eefu \u00edch cho nghi\u00ean c\u1ee9u v\u1ec1 vi\u1ec7c s\u1eed d\u1ee5ng t\u1ebf b\u00e0o NK cho li\u1ec7u ph\u00e1p mi\u1ec5n d\u1ecbch nu\u00f4i c\u1ea5y c\u0169ng nh\u01b0 nghi\u00ean c\u1ee9u v\u1ec1 sinh h\u1ecdc t\u1ebf b\u00e0o NK c\u01a1 b\u1ea3n.&nbsp;<\/p>\n<p>StemSpan\u2122 NK Cell Generation Kit l\u00e0 m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y serum free, feeder-free gi\u00fap bi\u1ec7t h\u00f3a t\u1ebf b\u00e0o CD34+ \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb m\u00e1u d\u00e2y r\u1ed1n ho\u1eb7c t\u1ee7y x\u01b0\u01a1ng th\u00e0nh t\u1ebf b\u00e0o NK. Tham kh\u1ea3o ph\u1ea7n 4 \u0111\u1ec3 bi\u1ebft quy tr\u00ecnh bi\u1ec7t h\u00f3a.&nbsp;<\/p>\n<p>StemSpan\u2122 NK Cell Generation Kit c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u1edbi t\u1ebf b\u00e0o CD34+ t\u01b0\u01a1i ho\u1eb7c \u0111\u00f4ng l\u1ea1nh thu \u0111\u01b0\u1ee3c t\u1eeb CB ho\u1eb7c BM, ho\u1eb7c c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb hPSC. Trong b\u1ed9 kit n\u00e0y, t\u1ebf b\u00e0o CD34+ \u0111\u1ea7u ti\u00ean \u0111\u01b0\u1ee3c bi\u1ec7t h\u00f3a th\u00e0nh t\u1ebf b\u00e0o ti\u1ec1n th\u00e2n lymphoid CD7+CD5+, sau \u0111\u00f3 \u0111\u01b0\u1ee3c \u0111\u1ecbnh h\u01b0\u1edbng bi\u1ec7t h\u00f3a th\u00e0nh t\u1ebf b\u00e0o NK CD56+.&nbsp;<\/p>\n<p><a href=\"https:\/\/www.stemcell.com\/products\/stemspan-nk-cell-generation-kit.html\"><strong>StemSpan\u2122 NK Cell Generation Kit (#09960)<\/strong><\/a>&nbsp;<\/p>\n<figure class=\"table\">\n<table>\n<tbody>\n<tr>\n<td><strong>S\u1ea3n ph\u1ea9m<\/strong>&nbsp;<\/td>\n<td>\n<p style=\"text-align:center\"><strong>Quy c\u00e1ch<\/strong>&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\"><strong>M\u00e3 s\u1ea3n ph\u1ea9m<\/strong>&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>StemSpan\u2122 Lymphoid Progenitor Expansion Supplement (10X)&nbsp;<\/td>\n<td>\n<p style=\"text-align:center\">5 mL&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\"><a href=\"https:\/\/www.stemcell.com\/products\/stemspan-lymphoid-progenitor-expansion-supplement-10x.html\">09915<\/a>&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>StemSpan\u2122 Lymphoid Differentiation Coating Material (100X)&nbsp;<\/td>\n<td>\n<p style=\"text-align:center\">250 \u00b5L&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\"><a href=\"https:\/\/www.stemcell.com\/products\/stemspan-lymphoid-differentiation-coating-material-100x.html\">09925<\/a>&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>StemSpan\u2122 NK Cell Differentiation Supplement (100X)&nbsp;<\/td>\n<td>\n<p style=\"text-align:center\">0.5 mL&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\"><a href=\"https:\/\/www.stemcell.com\/products\/stemspan-nk-cell-differentiation-supplement-100x.html\">09950<\/a>&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>StemSpan\u2122 SFEM II&nbsp;<\/td>\n<td>\n<p style=\"text-align:center\">100 mL&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\"><a href=\"https:\/\/www.stemcell.com\/products\/stemspan-sfem-ii.html\">09605<\/a>&nbsp;<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<h4><span class=\"ez-toc-section\" id=\"2_Hoa_chat_vat_tu\"><\/span><strong>2. H\u00f3a ch\u1ea5t, v\u1eadt t\u01b0<\/strong>&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h4>\n<figure class=\"table\">\n<table>\n<tbody>\n<tr>\n<td>\n<p style=\"text-align:center\"><strong>S\u1ea3n ph\u1ea9m<\/strong>&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\"><strong>M\u00e3 s\u1ea3n ph\u1ea9m<\/strong>&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>D-PBS (Without Ca++ and Mg++)&nbsp;<\/td>\n<td>\n<p style=\"text-align:center\"><a href=\"https:\/\/www.stemcell.com\/products\/d-pbs-without-ca-and-mg.html\">37350<\/a>&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>Trypan Blue&nbsp;<\/td>\n<td>\n<p style=\"text-align:center\"><a href=\"https:\/\/www.stemcell.com\/products\/trypan-blue.html\">07050<\/a>&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>UM729&nbsp;<\/td>\n<td>\n<p style=\"text-align:center\"><a href=\"https:\/\/www.stemcell.com\/um729.html\">72332<\/a>&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>Non-tissue culture-treated cultureware&nbsp;<\/td>\n<td>\n<p style=\"text-align:center\">21024010 (24 wells)&nbsp;<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<h4><span class=\"ez-toc-section\" id=\"3_Thiet_bi_dung_cu\"><\/span><strong>3. Thi\u1ebft b\u1ecb, d\u1ee5ng c\u1ee5<\/strong>&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h4>\n<ul>\n<li>T\u1ee7 an to\u00e0n sinh h\u1ecdc c\u1ea5p II&nbsp;<\/li>\n<li>T\u1ee7 \u1ea5m c\u00f3 ki\u1ec3m so\u00e1t \u0111\u1ed9 \u1ea9m v\u00e0 kh\u00ed \u0111\u1ec3 duy tr\u00ec 37\u00b0C, \u0111\u1ed9 \u1ea9m 95% v\u00e0 5% CO2.&nbsp;<\/li>\n<li>M\u00e1y ly t\u00e2m t\u1ed1c \u0111\u1ed9 th\u1ea5p v\u1edbi roto v\u0103ng c\u00f3 gi\u00e1 \u0111\u1ee1 \u0111\u0129a&nbsp;<\/li>\n<li>Pipette-aid&nbsp;<\/li>\n<li>Bu\u1ed3ng \u0111\u1ebfm h\u1ed3ng c\u1ea7u&nbsp;<\/li>\n<li>Micropipettor&nbsp;&nbsp;<\/li>\n<li>K\u00ednh hi\u1ec3n vi soi ng\u01b0\u1ee3c&nbsp;<\/li>\n<li>Flow cytometer&nbsp;<\/li>\n<li>T\u1ee7 \u0111\u00f4ng -20\u00b0C&nbsp;<\/li>\n<li>T\u1ee7 l\u1ea1nh (2 &#8211; 8\u00b0C) &nbsp;<\/li>\n<\/ul>\n<h4><span class=\"ez-toc-section\" id=\"4_Biet_hoa_thanh_te_bao_NK\"><\/span><strong>4. Bi\u1ec7t h\u00f3a th\u00e0nh t\u1ebf b\u00e0o NK<\/strong>&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h4>\n<p>Quy tr\u00ecnh sau \u0111\u00e2y d\u00f9ng \u0111\u1ec3 bi\u1ec7t h\u00f3a t\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb hPSC, c\u0169ng nh\u01b0 t\u1ebf b\u00e0o CD34+ \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb m\u00e1u d\u00e2y r\u1ed1n (CB) ho\u1eb7c t\u1ee7y x\u01b0\u01a1ng (BM) v\u1edbi t\u1ebf b\u00e0o NK.&nbsp;<\/p>\n<p><strong>4.1 S\u01a1 \u0111\u1ed3 quy tr\u00ecnh<\/strong>&nbsp;<\/p>\n<p style=\"text-align:center\"><img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/XyA3Cm9Md16Y9kpD4ARMtKSVJRXo4qQeCODl2Rf4.png\" width=\"483\" height=\"113\">&nbsp;<\/p>\n<p style=\"text-align:center\">H\u00ecnh 1. Quy tr\u00ecnh bi\u1ec7t h\u00f3a t\u1ebf b\u00e0o NK s\u1eed d\u1ee5ng StemSpan\u2122 NK Cell Generation Kit&nbsp;<\/p>\n<p><strong>4.2 Quy tr\u00ecnh bi\u1ec7t h\u00f3a<\/strong>&nbsp;<\/p>\n<p>C\u00e1c h\u01b0\u1edbng d\u1eabn sau \u0111\u00e2y d\u00e0nh cho m\u1ed9t gi\u1ebfng c\u1ee7a \u0111\u0129a 24 gi\u1ebfng. N\u1ebfu s\u1eed d\u1ee5ng v\u1eadt nu\u00f4i c\u1ea5y kh\u00e1c, h\u00e3y tham kh\u1ea3o B\u1ea3ng 1 v\u00e0 \u0111i\u1ec1u ch\u1ec9nh s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o v\u00e0 th\u1ec3 t\u00edch cho ph\u00f9 h\u1ee3p.&nbsp;<\/p>\n<p>\u0110\u1ec3 \u0111\u1ea1t hi\u1ec7u su\u1ea5t t\u1ed1i \u01b0u, h\u00e3y tu\u00e2n theo l\u1ecbch tr\u00ecnh nu\u00f4i c\u1ea5y v\u00e0 c\u1ea5y chuy\u1ec1n \u0111\u01b0\u1ee3c khuy\u1ebfn ngh\u1ecb. Tuy nhi\u00ean, l\u1ecbch tr\u00ecnh c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh khi c\u1ea7n thi\u1ebft, mi\u1ec5n l\u00e0 duy tr\u00ec kho\u1ea3ng c\u00e1ch nu\u00f4i c\u1ea5y t\u1eeb 3 &#8211; 4 ng\u00e0y.&nbsp;<\/p>\n<p><strong>Ng\u00e0y 0<\/strong>&nbsp;<\/p>\n<p>1. Th\u00eam 500 \u00b5L StemSpan\u2122 Lymphoid Differentiation Coating Material v\u00e0o m\u1ed7i gi\u1ebfng c\u1ee7a \u0111\u0129a 24 gi\u1ebfng kh\u00f4ng x\u1eed l\u00fd b\u1ec1 m\u1eb7t (#21024010). N\u1ebfu s\u1eed d\u1ee5ng v\u1eadt li\u1ec7u nu\u00f4i c\u1ea5y kh\u00e1c, h\u00e3y tham kh\u1ea3o B\u1ea3ng 1 \u0111\u1ec3 bi\u1ebft th\u1ec3 t\u00edch c\u1ea7n thi\u1ebft.&nbsp;<\/p>\n<p>B\u1ea3ng 1. Th\u1ec3 t\u00edch Coating Material v\u00e0 m\u00f4i tr\u01b0\u1eddng \u0111\u01b0\u1ee3c khuy\u1ebfn ngh\u1ecb v\u00e0 s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c khuy\u1ebfn ngh\u1ecb cho nhi\u1ec1u lo\u1ea1i v\u1eadt li\u1ec7u nu\u00f4i c\u1ea5y kh\u00e1c nhau&nbsp;&nbsp;<\/p>\n<figure class=\"table\">\n<table>\n<tbody>\n<tr>\n<td colspan=\"1\" rowspan=\"2\">\n<p style=\"text-align:center\">\u0110\u0129a nu\u00f4i c\u1ea5y kh\u00f4ng x\u1eed l\u00fd b\u1ec1 m\u1eb7t&nbsp;<\/p>\n<\/td>\n<td colspan=\"1\" rowspan=\"2\">\n<p style=\"text-align:center\">Th\u1ec3 t\u00edch Coating Material&nbsp;<\/p>\n<\/td>\n<td colspan=\"1\" rowspan=\"2\">\n<p style=\"text-align:center\">Th\u1ec3 t\u00edch m\u00f4i tr\u01b0\u1eddng t\u0103ng sinh ho\u1eb7c m\u00f4i tr\u01b0\u1eddng bi\u1ec7t h\u00f3a&nbsp;<\/p>\n<\/td>\n<td colspan=\"3\">\n<p style=\"text-align:center\">S\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o CD 34+\/gi\u1ebfng&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p style=\"text-align:center\">T\u1ebf b\u00e0o c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb CB&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">T\u1ebf b\u00e0o c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb BM&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">T\u1ebf b\u00e0o c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb hPSC*&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p style=\"text-align:center\">\u0110\u0129a 96 gi\u1ebfng (#21096010)&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">100 \u00b5L\/gi\u1ebfng&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">100 \u00b5L\/gi\u1ebfng&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">1 x 103&nbsp;<\/p>\n<p style=\"text-align:center\">&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">5 x 103&nbsp;<\/p>\n<p style=\"text-align:center\">&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">5 x 103&nbsp;<\/p>\n<p style=\"text-align:center\">&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p style=\"text-align:center\">\u0110\u0129a 12 gi\u1ebfng (#21012010)&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">1 mL\/gi\u1ebfng&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">1 mL\/gi\u1ebfng&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">1 x 104&nbsp;<\/p>\n<p style=\"text-align:center\">&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">5 x 104&nbsp;<\/p>\n<p style=\"text-align:center\">&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">5 x 104&nbsp;<\/p>\n<p style=\"text-align:center\">&nbsp;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p style=\"text-align:center\">\u0110\u0129a 6 gi\u1ebfng (#21006010)&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">2.5 mL\/gi\u1ebfng&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">2.5 mL\/gi\u1ebfng&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">2.5 x 104&nbsp;<\/p>\n<p style=\"text-align:center\">&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">1.25 x 105&nbsp;<\/p>\n<p style=\"text-align:center\">&nbsp;<\/p>\n<\/td>\n<td>\n<p style=\"text-align:center\">1.25 x 105&nbsp;<\/p>\n<p style=\"text-align:center\">&nbsp;<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<p>* T\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb hPSC \u0111\u01b0\u1ee3c t\u1ea1o ra b\u1eb1ng <a href=\"https:\/\/www.stemcell.com\/products\/stemdiff-nk-cell-kit.html\">STEMdiff\u2122 NK Cell Kit (#100-0170)<\/a>&nbsp;&nbsp;<\/p>\n<p>2. \u1ee6 \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng (15 &#8211; 25\u00b0C) trong 2 gi\u1edd.&nbsp;&nbsp;<\/p>\n<p>L\u01b0u \u00fd: Ho\u1eb7c \u1ee7 \u1edf nhi\u1ec7t \u0111\u1ed9 2 &#8211; 8\u00b0C qua \u0111\u00eam.&nbsp;<\/p>\n<p>3. H\u00fat Coating Material t\u1eeb \u0111\u0129a 24 gi\u1ebfng. R\u1eeda s\u1ea1ch gi\u1ebfng b\u1eb1ng D-PBS (Kh\u00f4ng c\u00f3 Ca++ v\u00e0 Mg++). H\u00fat D-PBS ngay tr\u01b0\u1edbc khi s\u1eed d\u1ee5ng.&nbsp;<\/p>\n<p>4. Chu\u1ea9n b\u1ecb t\u1ebf b\u00e0o CD34+ nh\u01b0 sau:&nbsp;<\/p>\n<p>\u00b7 N\u1ebfu s\u1eed d\u1ee5ng t\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb hPSC t\u01b0\u01a1i, h\u00e3y ti\u1ebfn h\u00e0nh b\u01b0\u1edbc 5.&nbsp;<\/p>\n<p>\u00b7 N\u1ebfu s\u1eed d\u1ee5ng t\u1ebf b\u00e0o CD34+ \u0111\u00f4ng l\u1ea1nh t\u1eeb CB ho\u1eb7c BM c\u1ee7a ng\u01b0\u1eddi, h\u00e3y r\u00e3 \u0111\u00f4ng t\u1ebf b\u00e0o r\u1ed3i ti\u1ebfn h\u00e0nh b\u01b0\u1edbc 5.&nbsp;<\/p>\n<p>\u00b7 N\u1ebfu s\u1eed d\u1ee5ng CB ho\u1eb7c BM c\u1ee7a ng\u01b0\u1eddi t\u01b0\u01a1i (d\u01b0\u1edbi 72 gi\u1edd), h\u00e3y ph\u00e2n l\u1eadp t\u1ebf b\u00e0o CD34+ b\u1eb1ng m\u1ed9t trong c\u00e1c b\u1ed9 d\u1ee5ng c\u1ee5 EasySep \u2122 \u0111\u01b0\u1ee3c li\u1ec7t k\u00ea b\u00ean d\u01b0\u1edbi, sau \u0111\u00f3 ti\u1ebfn h\u00e0nh b\u01b0\u1edbc 5.&nbsp;<\/p>\n<p>o CB: <a href=\"https:\/\/www.stemcell.com\/easysep-human-cord-blood-cd34-positive-selection-kit-ii.html\">EasySep\u2122 Human Cord Blood CD34 Positive Selection Kit II (#17896)<\/a>&nbsp;&nbsp;<\/p>\n<p>o BM: <a href=\"https:\/\/www.stemcell.com\/easysep-human-cd34-positive-selection-kit-ii.html\">EasySep\u2122 Human CD34 Positive Selection Kit II (#17856)<\/a>&nbsp;<\/p>\n<p>5. Th\u1ef1c hi\u1ec7n \u0111\u1ebfm t\u1ebf b\u00e0o s\u1ed1ng b\u1eb1ng Trypan Blue v\u00e0 bu\u1ed3ng \u0111\u1ebfm h\u1ed3ng c\u1ea7u. X\u00e1c \u0111\u1ecbnh % t\u1ebf b\u00e0o CD34+ b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p flow cytometry, s\u1eed d\u1ee5ng m\u1ed9t trong c\u00e1c kh\u00e1ng th\u1ec3 li\u00ean h\u1ee3p v\u1edbi fluorochrome sau:&nbsp;<\/p>\n<p>\u00b7 <a href=\"https:\/\/www.stemcell.com\/anti-human-cd34-antibody-clone-581.html\">Anti-Human CD34 Antibody, Clone 581 (#60013)<\/a>&nbsp;<\/p>\n<p>\u00b7 <a href=\"https:\/\/www.stemcell.com\/anti-human-cd34-antibody-clone-8g12.html\">Anti-Human CD34 Antibody, Clone 8G12 (#60121)<\/a>&nbsp;<\/p>\n<p>\u0110\u1ec3 x\u00e1c \u0111\u1ecbnh n\u1ed3ng \u0111\u1ed9 t\u1ebf b\u00e0o CD34+, nh\u00e2n % t\u1ebf b\u00e0o CD34+ v\u1edbi s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o s\u1ed1ng.&nbsp;<\/p>\n<p>L\u01b0u \u00fd: T\u1ef7 l\u1ec7 t\u1ebf b\u00e0o CD34+ d\u1ef1 ki\u1ebfn l\u00e0 &gt; 50%.&nbsp;<\/p>\n<p>N\u1ebfu t\u1ea7n su\u1ea5t t\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb hPSC sau khi t\u00e1ch t\u1ebf b\u00e0o &lt; 50%, vi\u1ec7c t\u00e1ch th\u00eam b\u1eb1ng nam ch\u00e2m c\u00f3 th\u1ec3 c\u1ea3i thi\u1ec7n k\u1ebft qu\u1ea3.&nbsp;&nbsp;<\/p>\n<p>6. Th\u00eam t\u1ebf b\u00e0o CD34+ (t\u1eeb b\u01b0\u1edbc 4) v\u00e0o 500 \u00b5L StemSpan\u2122 Lymphoid Progenitor Expansion Medium nh\u01b0 sau:&nbsp;<\/p>\n<p>\u00b7 T\u1ebf b\u00e0o c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb CB: 1 x 104 t\u1ebf b\u00e0o CD34+\/mL (5 x 103 t\u1ebf b\u00e0o CD34+\/gi\u1ebfng)&nbsp;<\/p>\n<p>\u00b7 T\u1ebf b\u00e0o c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb BM ho\u1eb7c hPSC: 5 x 104 t\u1ebf b\u00e0o CD34+\/mL (2.5 x 104 t\u1ebf b\u00e0o CD34+\/gi\u1ebfng)&nbsp;<\/p>\n<p>L\u01b0u \u00fd: Dung d\u1ecbch huy\u1ec1n ph\u00f9 t\u1ebf b\u00e0o n\u00e0y d\u00e0nh cho m\u1ed9t gi\u1ebfng c\u1ee7a \u0111\u0129a 24 gi\u1ebfng. N\u1ebfu s\u1eed d\u1ee5ng v\u1eadt nu\u00f4i c\u1ea5y kh\u00e1c, h\u00e3y tham kh\u1ea3o B\u1ea3ng 1 \u0111\u1ec3 bi\u1ebft th\u1ec3 t\u00edch v\u00e0 s\u1ed1 l\u01b0\u1ee3ng t\u1ebf b\u00e0o c\u1ea7n thi\u1ebft.&nbsp;<\/p>\n<p>7. Th\u00eam 500 \u00b5L dung d\u1ecbch huy\u1ec1n ph\u00f9 t\u1ebf b\u00e0o (\u0111\u00e3 chu\u1ea9n b\u1ecb \u1edf b\u01b0\u1edbc 6) v\u00e0o m\u1ed9t gi\u1ebfng \u0111\u00e3 ph\u1ee7 c\u1ee7a \u0111\u0129a 24 gi\u1ebfng \u0111\u00e3 chu\u1ea9n b\u1ecb \u1edf b\u01b0\u1edbc 1 &#8211; 3. \u1ee6 \u1edf 37\u00b0C v\u00e0 5% CO2 trong 3 ho\u1eb7c 4 ng\u00e0y.&nbsp;<\/p>\n<p><strong>Ng\u00e0y 3 ho\u1eb7c 4<\/strong>&nbsp;<\/p>\n<p>8. Th\u00eam t\u1eeb t\u1eeb 500 \u00b5L StemSpan\u2122 Lymphoid Progenitor Expansion Medium v\u00e0o m\u1ed7i gi\u1ebfng c\u1ee7a \u0111\u0129a 24 gi\u1ebfng. \u1ee6 \u1edf 37\u00b0C v\u00e0 5% CO2 trong 3 ho\u1eb7c 4 ng\u00e0y.&nbsp;<\/p>\n<p>L\u01b0u \u00fd: N\u1ebfu s\u1eed d\u1ee5ng t\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb hPSC, h\u00e3y theo d\u00f5i qu\u00e1 tr\u00ecnh nu\u00f4i c\u1ea5y \u0111\u1ec3 ph\u00e1t tri\u1ec3n c\u00e1c t\u1ebf b\u00e0o b\u00e1m d\u00ednh. N\u1ebfu c\u00e1c t\u1ebf b\u00e0o b\u00e1m d\u00ednh d\u01b0\u1eddng nh\u01b0 \u0111\u1ea1t \u0111\u1ebfn &gt; 50% h\u1ee3p l\u01b0u, n\u00ean th\u1ef1c hi\u1ec7n b\u01b0\u1edbc 9b: \u201c\u0110\u1ed1i v\u1edbi t\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb hPSC\u201d s\u1edbm \u0111\u1ec3 duy tr\u00ec hi\u1ec7u su\u1ea5t t\u1ed1i \u01b0u.&nbsp;&nbsp;<\/p>\n<p><strong>Ng\u00e0y 7<\/strong>&nbsp;<\/p>\n<p>9. Th\u1ef1c hi\u1ec7n thay m\u1ed9t n\u1eeda m\u00f4i tr\u01b0\u1eddng nh\u01b0 sau:&nbsp;<\/p>\n<p>a. C\u1ea9n th\u1eadn h\u00fat 500 \u00b5L m\u00f4i tr\u01b0\u1eddng ra kh\u1ecfi gi\u1ebfng. Kh\u00f4ng l\u00e0m x\u00e1o tr\u1ed9n t\u1ebf b\u00e0o.&nbsp;<\/p>\n<p>b. Th\u00eam 500 \u00b5L StemSpan\u2122 Lymphoid Progenitor Expansion Medium v\u00e0o m\u1ed7i gi\u1ebfng.&nbsp;<\/p>\n<p>\u00b7 \u0110\u1ed1i v\u1edbi t\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb CB ho\u1eb7c BM c\u1ee7a con ng\u01b0\u1eddi: \u1ee6 \u1edf 37\u00b0C trong 3 ho\u1eb7c 4 ng\u00e0y, sau \u0111\u00f3 ti\u1ebfn h\u00e0nh b\u01b0\u1edbc 10.&nbsp;<\/p>\n<p>\u00b7 \u0110\u1ed1i v\u1edbi t\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb hPSC:&nbsp;<\/p>\n<p>i. Chu\u1ea9n b\u1ecb m\u1ed9t \u0111\u0129a 24 gi\u1ebfng nh\u01b0 m\u00f4 t\u1ea3 \u1edf c\u00e1c b\u01b0\u1edbc 1 &#8211; 3.&nbsp;<\/p>\n<p>ii. Nh\u1eb9 nh\u00e0ng d\u00f9ng pipet h\u00fat l\u00ean xu\u1ed1ng trong gi\u1ebfng \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o t\u1ea5t c\u1ea3 c\u00e1c t\u1ebf b\u00e0o \u0111\u1ec1u \u1edf tr\u1ea1ng th\u00e1i huy\u1ec1n ph\u00f9.&nbsp;<\/p>\n<p>L\u01b0u \u00fd: Kh\u00f4ng c\u1ea1o \u0111\u00e1y \u0111\u0129a ho\u1eb7c huy\u1ec1n ph\u00f9 qu\u00e1 m\u1ea1nh v\u00ec \u0111i\u1ec1u n\u00e0y s\u1ebd l\u00e0m bong ra b\u1ea5t k\u1ef3 t\u1ebf b\u00e0o b\u00e1m d\u00ednh n\u00e0o \u0111\u00e3 ph\u00e1t tri\u1ec3n.&nbsp;<\/p>\n<p>iii. Chuy\u1ec3n d\u1ecbch huy\u1ec1n ph\u00f9 t\u1eeb m\u1ed9t gi\u1ebfng sang m\u1ed9t gi\u1ebfng c\u1ee7a \u0111\u0129a m\u1edbi ph\u1ee7. L\u1eb7p l\u1ea1i cho t\u1ea5t c\u1ea3 c\u00e1c gi\u1ebfng.&nbsp;<\/p>\n<p>iv. \u1ee6 \u1edf nhi\u1ec7t \u0111\u1ed9 37\u00b0C v\u00e0 5% CO2 trong 3 ho\u1eb7c 4 ng\u00e0y, sau \u0111\u00f3 ti\u1ebfn h\u00e0nh b\u01b0\u1edbc 10.&nbsp;<\/p>\n<p><strong>Ng\u00e0y 10 ho\u1eb7c 11<\/strong>&nbsp;<\/p>\n<p>10. Th\u1ef1c hi\u1ec7n thay m\u1ed9t n\u1eeda m\u00f4i tr\u01b0\u1eddng nh\u01b0 sau:&nbsp;<\/p>\n<p>a. C\u1ea9n th\u1eadn h\u00fat 500 \u00b5L m\u00f4i tr\u01b0\u1eddng ra kh\u1ecfi gi\u1ebfng. Kh\u00f4ng l\u00e0m x\u00e1o tr\u1ed9n t\u1ebf b\u00e0o.&nbsp;<\/p>\n<p>b. Th\u00eam 500 \u00b5L StemSpan\u2122 Lymphoid Progenitor Expansion Medium v\u00e0o m\u1ed7i gi\u1ebfng. \u1ee6 \u1edf 37\u00b0C v\u00e0 5% CO2 trong 3 ho\u1eb7c 4 ng\u00e0y.&nbsp;<\/p>\n<p>L\u01b0u \u00fd: N\u1ebfu t\u1ebf b\u00e0o \u0111\u1ea1t \u0111\u1ebfn \u0111\u1ed9 h\u1ee3p l\u01b0u tr\u01b0\u1edbc khi thu ho\u1ea1ch ho\u1eb7c m\u00f4i tr\u01b0\u1eddng thay \u0111\u1ed5i trong ph\u1ea7n c\u00f2n l\u1ea1i c\u1ee7a quy tr\u00ecnh, h\u00e3y gi\u1ea3m m\u1eadt \u0111\u1ed9 t\u1ebf b\u00e0o b\u1eb1ng c\u00e1ch h\u00fat l\u00ean v\u00e0 xu\u1ed1ng, lo\u1ea1i b\u1ecf m\u1ed9t n\u1eeda m\u00f4i tr\u01b0\u1eddng c\u00f3 ch\u1ee9a t\u1ebf b\u00e0o v\u00e0 thay th\u1ebf b\u1eb1ng m\u00f4i tr\u01b0\u1eddng m\u1edbi.&nbsp;<\/p>\n<p><strong>Ng\u00e0y 14 &#8211; Thu t\u1ebf b\u00e0o v\u00e0 c\u1ea5y chuy\u1ec1n<\/strong>&nbsp;<\/p>\n<p>11. Nh\u1eb9 nh\u00e0ng h\u00fat c\u00e1c t\u1ebf b\u00e0o l\u00ean xu\u1ed1ng trong gi\u1ebfng \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o t\u1ea5t c\u1ea3 c\u00e1c t\u1ebf b\u00e0o \u0111\u1ec1u \u1edf tr\u1ea1ng th\u00e1i l\u01a1 l\u1eedng. Chuy\u1ec3n c\u00e1c t\u1ebf b\u00e0o v\u00e0o m\u1ed9t \u1ed1ng th\u00edch h\u1ee3p; c\u00e1c t\u1ebf b\u00e0o n\u00e0y bao g\u1ed3m c\u00e1c t\u1ebf b\u00e0o ti\u1ec1n th\u00e2n lymphoid.&nbsp;<\/p>\n<p>L\u01b0u \u00fd: T\u1ebf b\u00e0o c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh \u1edf giai \u0111o\u1ea1n n\u00e0y b\u1eb1ng <a href=\"https:\/\/www.stemcell.com\/products\/cryostor-cs10.html\">CryoStor \u00ae CS10 (#07930)<\/a>. Tham kh\u1ea3o PIS cho CryoStor \u00ae CS10 \u0111\u1ec3 bi\u1ebft h\u01b0\u1edbng d\u1eabn b\u1ea3o qu\u1ea3n \u0111\u00f4ng l\u1ea1nh v\u00e0 r\u00e3 \u0111\u00f4ng. Sau khi t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c r\u00e3 \u0111\u00f4ng, h\u00e3y ti\u1ebfn h\u00e0nh b\u01b0\u1edbc 12.&nbsp;<\/p>\n<p>12. Th\u1ef1c hi\u1ec7n \u0111\u1ebfm t\u1ebf b\u00e0o s\u1ed1ng b\u1eb1ng Trypan Blue v\u00e0 bu\u1ed3ng \u0111\u1ebfm h\u1ed3ng c\u1ea7u.&nbsp;<\/p>\n<p>13. Th\u00eam t\u1ebf b\u00e0o \u1edf m\u1ee9c 1 x 105 t\u1ebf b\u00e0o\/mL v\u00e0o 500 \u00b5L StemSpan\u2122 NK Cell Differentiation Medium.&nbsp;<\/p>\n<p>L\u01b0u \u00fd: Dung d\u1ecbch t\u1ebf b\u00e0o n\u00e0y d\u00e0nh cho m\u1ed9t gi\u1ebfng c\u1ee7a \u0111\u0129a 24 gi\u1ebfng. N\u1ebfu s\u1eed d\u1ee5ng d\u1ee5ng c\u1ee5 nu\u00f4i c\u1ea5y kh\u00e1c, h\u00e3y tham kh\u1ea3o B\u1ea3ng 1 \u0111\u1ec3 bi\u1ebft th\u1ec3 t\u00edch c\u1ea7n thi\u1ebft.&nbsp;<\/p>\n<p>14. Th\u00eam 500 \u00b5L dung d\u1ecbch huy\u1ec1n ph\u00f9 t\u1ebf b\u00e0o (\u0111\u00e3 chu\u1ea9n b\u1ecb \u1edf b\u01b0\u1edbc 13) v\u00e0o m\u1ed9t gi\u1ebfng (5&#215;104 t\u1ebf b\u00e0o\/gi\u1ebfng) c\u1ee7a \u0111\u0129a 24 gi\u1ebfng kh\u00f4ng x\u1eed l\u00fd b\u1ec1 m\u1eb7t (#21024010). \u1ee6 \u1edf 37\u00b0C v\u00e0 5% CO2 trong 3 ho\u1eb7c 4 ng\u00e0y.&nbsp;<\/p>\n<p><strong>Ng\u00e0y 17 ho\u1eb7c 18<\/strong>&nbsp;<\/p>\n<p>15. Th\u00eam t\u1eeb t\u1eeb 500 \u00b5L StemSpan\u2122 NK Cell Differentiation Medium v\u00e0o m\u1ed7i gi\u1ebfng. \u1ee6 \u1edf 37\u00b0C v\u00e0 5% CO2 trong 3 ho\u1eb7c 4 ng\u00e0y.&nbsp;<\/p>\n<p><strong>Ng\u00e0y 21<\/strong>&nbsp;<\/p>\n<p>16. Th\u1ef1c hi\u1ec7n thay m\u1ed9t n\u1eeda m\u00f4i tr\u01b0\u1eddng nh\u01b0 sau:&nbsp;<\/p>\n<p>a. C\u1ea9n th\u1eadn h\u00fat 500 \u00b5L m\u00f4i tr\u01b0\u1eddng ra kh\u1ecfi gi\u1ebfng. Kh\u00f4ng l\u00e0m x\u00e1o tr\u1ed9n t\u1ebf b\u00e0o.&nbsp;<\/p>\n<p>b. Th\u00eam 500 \u00b5L StemSpan\u2122 NK Cell Differentiation Medium v\u00e0o m\u1ed7i gi\u1ebfng. \u1ee6 \u1edf 37\u00b0C v\u00e0 5% CO2 trong 3 ho\u1eb7c 4 ng\u00e0y.&nbsp;<\/p>\n<p><strong>Ng\u00e0y 24 ho\u1eb7c 25<\/strong>&nbsp;<\/p>\n<p>17. Th\u1ef1c hi\u1ec7n thay m\u1ed9t n\u1eeda m\u00f4i tr\u01b0\u1eddng nh\u01b0 sau:&nbsp;<\/p>\n<p>a. C\u1ea9n th\u1eadn l\u1ea5y 500 \u00b5L m\u00f4i tr\u01b0\u1eddng ra kh\u1ecfi gi\u1ebfng. Kh\u00f4ng l\u00e0m x\u00e1o tr\u1ed9n t\u1ebf b\u00e0o.&nbsp;<\/p>\n<p>b. Th\u00eam 500 \u00b5L StemSpan\u2122 NK Cell Differentiation Medium v\u00e0o m\u1ed7i gi\u1ebfng. \u1ee6 \u1edf 37\u00b0C v\u00e0 5% CO2 trong 3 ho\u1eb7c 4 ng\u00e0y.&nbsp;<\/p>\n<p><strong>Ng\u00e0y 28 &#8211; Thu ho\u1ea1ch t\u1ebf b\u00e0o<\/strong>&nbsp;<\/p>\n<p>18. Nh\u1eb9 nh\u00e0ng h\u00fat t\u1ebf b\u00e0o l\u00ean xu\u1ed1ng \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o t\u1ea5t c\u1ea3 c\u00e1c t\u1ebf b\u00e0o \u0111\u1ec1u \u1edf tr\u1ea1ng th\u00e1i huy\u1ec1n ph\u00f9. Chuy\u1ec3n t\u1ebf b\u00e0o v\u00e0o \u1ed1ng th\u00edch h\u1ee3p. C\u00e1c t\u1ebf b\u00e0o NK n\u00e0y \u0111\u00e3 s\u1eb5n s\u00e0ng \u0111\u1ec3 x\u00e9t nghi\u1ec7m ho\u1eb7c ph\u00e2n t\u00edch khi c\u1ea7n thi\u1ebft.&nbsp;<\/p>\n<p><strong>4.3 \u0110\u00e1nh gi\u00e1 ki\u1ec3u h\u00ecnh<\/strong>&nbsp;<\/p>\n<p>\u0110\u1ec3 \u0111\u00e1nh gi\u00e1 ki\u1ec3u h\u00ecnh c\u1ee7a t\u1ebf b\u00e0o ti\u1ec1n th\u00e2n lymphoid b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p flow cytometry, h\u00e3y s\u1eed d\u1ee5ng c\u00e1c kh\u00e1ng th\u1ec3 li\u00ean h\u1ee3p hu\u1ef3nh quang sau:&nbsp;<\/p>\n<p>\u00b7 <a href=\"https:\/\/www.stemcell.com\/anti-human-cd5-antibody-clone-ucht2.html\">Anti-Human CD5 Antibody, UCHT2 (#60082)<\/a>&nbsp;<\/p>\n<p>\u00b7 Anti-Human CD7 Antibody, Clone CD7-6B7&nbsp;<\/p>\n<p>\u0110\u1ec3 \u0111\u00e1nh gi\u00e1 ki\u1ec3u h\u00ecnh c\u1ee7a t\u1ebf b\u00e0o NK b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p flow cytometry, h\u00e3y s\u1eed d\u1ee5ng c\u00e1c kh\u00e1ng th\u1ec3 li\u00ean h\u1ee3p v\u1edbi hu\u1ef3nh quang sau \u0111\u00e2y:&nbsp;<\/p>\n<p>\u00b7 <a href=\"https:\/\/www.stemcell.com\/anti-human-cd16-antibody-clone-3g8.html\">Anti-Human CD16 Antibody, Clone 3G8 (#60041)<\/a>&nbsp;&nbsp;<\/p>\n<p>\u00b7 <a href=\"https:\/\/www.stemcell.com\/anti-human-cd56-ncam-antibody-clone-hcd56.html\">Anti-Human CD56 Antibody, Clone HCD56 (#60021)<\/a>&nbsp;&nbsp;<\/p>\n<p>\u00b7 Anti-human CD94 Antibody, Clone DX22&nbsp;&nbsp;<\/p>\n<p>\u00b7 Anti-human CD158 (KIR) Antibody, Clone 180704 and\/or HP-MA4&nbsp;&nbsp;<\/p>\n<p>\u00b7 Anti-human CD335 (NKp46) Antibody, Clone 9E2&nbsp;&nbsp;<\/p>\n<p>\u00b7 Anti-human CD336 (NKp44) Antibody, Clone P44-8&nbsp;&nbsp;<\/p>\n<p>\u00b7 Anti-human CD337 (NKp30) Antibody, Clone P30-15&nbsp;&nbsp;<\/p>\n<p>\u00b7 Anti-human NKG2D Antibody, Clone 1D11&nbsp;<\/p>\n<h4><span class=\"ez-toc-section\" id=\"5_Du_lieu\"><\/span><strong>5. D\u1eef li\u1ec7u<\/strong>&nbsp;<span class=\"ez-toc-section-end\"><\/span><\/h4>\n<p style=\"text-align:center\"><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/n1Ol1F5nrbThdnPsPp48HBVSEO3Th3gdZwa77H9V.png\" alt=\"Frequency and yield of CD56- and NKp46-expressing cells produced after culture of CD34+ HSPCs with the StemSpan\u2122 NK Cell Generation Kit\" width=\"417\" height=\"187\">&nbsp;<\/p>\n<p style=\"text-align:center\">H\u00ecnh 2. T\u1ea7n su\u1ea5t v\u00e0 hi\u1ec7u su\u1ea5t t\u1ebf b\u00e0o NK CD56+ sau 28 ng\u00e0y nu\u00f4i c\u1ea5y&nbsp;<\/p>\n<p>T\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb CB (m\u1edbi ph\u00e2n l\u1eadp ho\u1eb7c \u0111\u00f4ng l\u1ea1nh) \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y b\u1eb1ng StemSpan\u2122 NK Cell Generation Kit trong 28 ng\u00e0y. C\u00e1c t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c thu ho\u1ea1ch v\u00e0 ph\u00e2n t\u00edch bi\u1ec3u hi\u1ec7n (A, B) CD56 v\u00e0 (A) NKp46 b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p flow cytometry. C\u00e1c t\u1ebf b\u00e0o ch\u1ebft \u0111\u01b0\u1ee3c lo\u1ea1i tr\u1eeb b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p t\u00e1n x\u1ea1 \u00e1nh s\u00e1ng v\u00e0 nhu\u1ed9m m\u00e0u t\u1ebf b\u00e0o s\u1ed1ng. (B) T\u1ea7n su\u1ea5t t\u1ebf b\u00e0o s\u1ed1ng trung b\u00ecnh c\u1ee7a t\u1ebf b\u00e0o NK CD56+ v\u00e0o ng\u00e0y th\u1ee9 28 l\u00e0 77%, v\u1edbi ~9.000 t\u1ebf b\u00e0o CD56+ \u0111\u01b0\u1ee3c s\u1ea3n xu\u1ea5t tr\u00ean m\u1ed7i t\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb CB \u0111\u1ea7u v\u00e0o. Hi\u1ec3n th\u1ecb l\u00e0 gi\u00e1 tr\u1ecb trung b\u00ecnh v\u1edbi kho\u1ea3ng tin c\u1eady 95% (n = 45: 23 m\u1eabu t\u1ebf b\u00e0o m\u1edbi ph\u00e2n l\u1eadp v\u00e0 22 m\u1eabu t\u1ebf b\u00e0o CD34+ \u0111\u00f4ng l\u1ea1nh). C\u00e1c t\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb BM c\u0169ng \u0111\u01b0\u1ee3c bi\u1ec7t h\u00f3a th\u00e0nh c\u00e1c t\u1ebf b\u00e0o NK b\u1eb1ng StemSpan\u2122 NK Cell Generation Kit. Hi\u1ec7u su\u1ea5t c\u1ee7a t\u1ebf b\u00e0o NK t\u1eeb BM HSPC th\u01b0\u1eddng th\u1ea5p h\u01a1n so v\u1edbi CB, trung b\u00ecnh kho\u1ea3ng 75 tr\u00ean m\u1ed7i t\u1ebf b\u00e0o CD34+ \u0111\u1ea7u v\u00e0o (n = 3, d\u1eef li\u1ec7u kh\u00f4ng \u0111\u01b0\u1ee3c hi\u1ec3n th\u1ecb).&nbsp;<\/p>\n<p style=\"text-align:center\"><img decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/Pjw0ddQ5EDKHR418hAlNkH866Lgf1Jyg6xJDiZsR.png\" alt=\"Flow cytometry plots showing cell surface marker expression on CD56+ NK cells after culture with the StemSpan\u2122 NK Cell Generation Kit\" width=\"509\" height=\"316\">&nbsp;<\/p>\n<p style=\"text-align:center\">H\u00ecnh 3. Bi\u1ec3u hi\u1ec7n d\u1ea5u hi\u1ec7u b\u1ec1 m\u1eb7t t\u1ebf b\u00e0o tr\u00ean c\u00e1c t\u1ebf b\u00e0o NK CD56+ sau 28 ng\u00e0y nu\u00f4i c\u1ea5y&nbsp;<\/p>\n<p>C\u00e1c t\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb CB \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y b\u1eb1ng StemSpan\u2122 NK Cell Generation Kit trong 28 ng\u00e0y. C\u00e1c t\u1ebf b\u00e0o \u0111\u00e3 bi\u1ec7t h\u00f3a \u0111\u01b0\u1ee3c thu ho\u1ea1ch v\u00e0 ph\u00e2n t\u00edch b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p flow cytometry cho bi\u1ec3u hi\u1ec7n CD56, NKp44, NKp30, NKG2D, CD94, CD16 v\u00e0 KIR. Vi\u1ec7c nhu\u1ed9m c\u00e1c ph\u00e2n t\u1eed KIR \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n b\u1eb1ng c\u00e1ch k\u1ebft h\u1ee3p hai b\u1ea3n sao cho kh\u00e1ng th\u1ec3, 180704 v\u00e0 HP-MA4, v\u00ec m\u1ed7i b\u1ea3n sao nh\u1eadn di\u1ec7n m\u1ed9t t\u1eadp h\u1ee3p con ri\u00eang bi\u1ec7t c\u1ee7a c\u00e1c ph\u00e2n t\u1eed KIR.&nbsp;<\/p>\n<p style=\"text-align:center\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/biogroupvietnam.com\/wp-content\/uploads\/2025\/09\/toXnXf5rX6fdWCm8cv8XLBq3E7tarxRQkM200Lch.png\" alt=\"Killing activity of NK cells toward K562 target cells compared to PB NK cells following culture with the StemSpan\u2122 NK Cell Generation Kit\" width=\"512\" height=\"359\">&nbsp;<\/p>\n<p style=\"text-align:center\">H\u00ecnh 4. T\u1ebf b\u00e0o NK nu\u00f4i c\u1ea5y th\u1ec3 hi\u1ec7n \u0111\u1ed9c t\u00ednh \u0111\u1ed1i v\u1edbi d\u00f2ng t\u1ebf b\u00e0o K562&nbsp;<\/p>\n<p>T\u1ebf b\u00e0o NK \u0111\u01b0\u1ee3c t\u1ea1o ra t\u1eeb t\u1ebf b\u00e0o CD34+ c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb CB trong 28 ng\u00e0y b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng quy tr\u00ecnh \u1edf H\u00ecnh 1. V\u00e0o ng\u00e0y 28, c\u00e1c t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c thu ho\u1ea1ch, nhu\u1ed9m cho CD56 v\u00e0 \u0111\u1ebfm c\u00e1c t\u1ebf b\u00e0o CD56+ s\u1ed1ng. C\u00e1c t\u1ebf b\u00e0o K562 \u0111\u01b0\u1ee3c \u1ee7 v\u1edbi 8 \u03bcM calcein AM \u1edf 37\u00b0C trong 1 gi\u1edd v\u00e0 sau \u0111\u00f3 r\u1eeda hai l\u1ea7n. Sau \u0111\u00f3, c\u00e1c t\u1ebf b\u00e0o NK CD56+ \u0111\u01b0\u1ee3c k\u1ebft h\u1ee3p v\u1edbi 10.000 t\u1ebf b\u00e0o m\u1ee5c ti\u00eau K562 \u0111\u01b0\u1ee3c g\u1eafn nh\u00e3n calcein AM n\u00e0y theo t\u1ef7 l\u1ec7 T\u00e1c nh\u00e2n: M\u1ee5c ti\u00eau l\u00e0 5:1 trong c\u00e1c \u0111\u0129a 96 gi\u1ebfng \u0111\u00e1y ch\u1eef U v\u00e0 \u0111\u1ed3ng nu\u00f4i c\u1ea5y (co-cultured) \u1edf 37\u00b0C trong 4 gi\u1edd. C\u00e1c t\u1ebf b\u00e0o NK m\u00e1u ngo\u1ea1i vi ng\u01b0\u1eddi l\u1edbn (adult) (PB) v\u00e0 c\u00e1c t\u1ebf b\u00e0o \u0111\u01a1n \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp b\u1eb1ng EasySep\u2122 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng l\u00e0m \u0111\u1ed1i ch\u1ee9ng d\u01b0\u01a1ng t\u00ednh v\u00e0 \u00e2m t\u00ednh. T\u1ebf b\u00e0o PB NK \u0111\u01b0\u1ee3c nu\u00f4i qua \u0111\u00eam v\u1edbi NK Cell Differentiation Supplement v\u00e0 SFEM II, trong khi c\u00e1c t\u1ebf b\u00e0o \u0111\u01a1n PB ch\u1ec9 \u0111\u01b0\u1ee3c nu\u00f4i qua \u0111\u00eam trong SFEM II. \u0110\u1ec3 ph\u00e1t hi\u1ec7n s\u1ef1 gi\u1ea3i ph\u00f3ng t\u1ef1 ph\u00e1t, ki\u1ec3m so\u00e1t c\u00e1c gi\u1ebfng \u0111\u1ed1i ch\u1ee9ng ch\u1ec9 ch\u1ee9a c\u00e1c t\u1ebf b\u00e0o m\u1ee5c ti\u00eau K562 \u0111\u01b0\u1ee3c g\u1eafn nh\u00e3n calcein AM \u0111\u00e3 \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp. C\u00e1c t\u1ebf b\u00e0o K562 \u0111\u01b0\u1ee3c g\u1eafn nh\u00e3n \u0111\u01b0\u1ee3c x\u1eed l\u00fd b\u1eb1ng 1% Triton\u2122 X-100 \u0111\u1ec3 \u0111o s\u1ef1 gi\u1ea3i ph\u00f3ng t\u1ed1i \u0111a. Sau khi \u1ee7, c\u00e1c \u0111\u0129a \u0111\u01b0\u1ee3c ly t\u00e2m \u1edf t\u1ed1c \u0111\u1ed9 500 x g trong 5 ph\u00fat v\u00e0 100 \u03bcL d\u1ecbch n\u1ed5i \u0111\u01b0\u1ee3c chuy\u1ec3n v\u00e0o c\u00e1c \u0111\u0129a \u0111en v\u00e0 ph\u00e2n t\u00edch b\u1eb1ng \u0111\u1ea7u \u0111\u1ecdc vi m\u1ea3ng SpectraMax\u00ae (k\u00edch th\u00edch 485 nm\/ph\u00e1t x\u1ea1 530 nm). K\u1ebft qu\u1ea3 \u0111\u01b0\u1ee3c bi\u1ec3u th\u1ecb d\u01b0\u1edbi d\u1ea1ng % ly gi\u1ea3i \u0111\u1eb7c hi\u1ec7u: [(th\u1eed nghi\u1ec7m gi\u1ea3i ph\u00f3ng &#8211; gi\u1ea3i ph\u00f3ng t\u1ef1 ph\u00e1t) x 100] \/ (gi\u1ea3i ph\u00f3ng t\u1ed1i \u0111a &#8211; gi\u1ea3i ph\u00f3ng t\u1ef1 ph\u00e1t). C\u00e1c t\u1ebf b\u00e0o NK c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb CB CD34+ cho th\u1ea5y ho\u1ea1t \u0111\u1ed9ng ti\u00eau di\u1ec7t t\u01b0\u01a1ng t\u1ef1 \u0111\u1ed1i v\u1edbi c\u00e1c t\u1ebf b\u00e0o \u0111\u00edch K562 so v\u1edbi c\u00e1c t\u1ebf b\u00e0o PB NK. Hi\u1ec3n th\u1ecb gi\u00e1 tr\u1ecb trung b\u00ecnh \u00b1 SD (t\u1ebf b\u00e0o NK c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb CB CD34+: n = 18, t\u1ebf b\u00e0o NK v\u00e0 t\u1ebf b\u00e0o \u0111\u01a1n PB: n = 7).<\/p>\n<p>Ngu\u1ed3n b\u00e0i vi\u1ebft | STEMCELL Technologies<\/p>\n","protected":false},"featured_media":5948,"parent":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-4439","service","type-service","status-publish","has-post-thumbnail","hentry","service-category-quy-trinh-huong-dan-su-dung"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Quy Tr\u00ecnh Nu\u00f4i C\u1ea5y V\u00e0 Bi\u1ec7t H\u00f3a T\u1ebf B\u00e0o NK S\u1eed D\u1ee5ng StemSpan\u2122 NK Cell Generation Kit<\/title>\n<meta name=\"description\" content=\"StemSpan\u2122 NK Cell Generation Kit l\u00e0 m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y serum free, feeder-free gi\u00fap bi\u1ec7t h\u00f3a t\u1ebf b\u00e0o CD34+ \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb m\u00e1u d\u00e2y r\u1ed1n ho\u1eb7c t\u1ee7y x\u01b0\u01a1ng th\u00e0nh t\u1ebf b\u00e0o NK. 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