CellCor™ EXO CD

Description

CellCor™ EXO CD – Chemically Defined, Serum-Free Medium for Exosome Collection from hMSC

Optimize EVs/Exosome purity and yield directly at the culture stage – a solution designed for R&D, QC, and preclinical production laboratories.
CellCor™ EXO CD is a serum-free, chemically defined (CD) medium specifically engineered for hMSC expansion and exosome collection, offering a flexible workflow (media exchange, direct seeding, or thawing directly in EXO CD).
The product contains no human- or animal-derived components and is quality-controlled for mycoplasma, sterility, endotoxin, and post-production particle count, effectively reducing serum-derived protein contaminants and improving the reliability of EVs analysis.

Why choose CellCor™ EXO CD?

• High purity: A superior protein-to-particle ratio compared with serum-containing media (NTA/BCA analysis, n=8). Representative images demonstrate a markedly lower background when using EXO CD.
• High EV particle yield: Data shown on page 1 of the brochure indicate a rapid increase in exosome particle concentration (particles/mL) over time after cell seeding using the EXO CD workflow, outperforming “Commercial Media A”.
• Compatible with functional assays: EXO CD supports downstream functional assays, including wound-healing (HaCaT) and angiogenesis (HUVEC) assays, following CM concentration and treatment at 1 × 10⁹ particles/mL, as illustrated by Incucyte images on page 2.
• Serum-free, chemically defined: Reduces lot-to-lot variability and the risk of exogenous contamination from FBS/d-FBS; suitable for standardized QC, NGS, and omics workflows, and for implementation in GMP-like (pre-manufacturing) environments.

Typical applications

• Expansion of hMSC prior to EVs/exosome collection
• Collection of EVs/exosomes from conditioned medium (CM) using enhanced recovery workflows
  (optional EXO CD replacement every 24–48 h for additional collections)
• Pre-processing for TFF/UF: CM concentrated 100× by TFF prior to functional evaluation

Biogroup can integrate CellCor™ EXO CD with EV concentration and isolation systems at R&D, pilot, and production scales (e.g., TFF lines and automated EV isolation platforms) to establish a standardized end-to-end workflow for customers.

Flexible workflows (choose 1 of 3)

1. Media Change Process: Thaw & expand cells in CellCor™ MSC CD AOF or conventional medium → (optional wash) → switch to CellCor™ EXO CD → enrich exosomes → collect CM (cell-conditioned medium) → isolate exosomes.
2. Seeding with EXO CD: Expand cells in MSC CD AOF → directly seed cells in EXO CD → enrich & collect CM → isolate exosomes.
3. Thawing with EXO CD: Thaw cells directly in EXO CD → expand → enrich & collect CM → isolate exosomes.

Technical recommendations (key requirements)

• Seeding density: 4,000–5,000 cells/cm²; collect CM at 75–85% confluence; isolate exosomes at 85–90% confluence
• Consumables: T-flasks, plates, Cell Factory.
• Medium volume:: T25 (5 mL), T75 (15 mL), T175 (30–40 mL), Cell Factory (100 mL–1 L).
• Antibiotics: Gentamicin hoặc Penicillin-Streptomycin (if required).
• Cell dissociation: TrypLE™ Express (no serum neutralization required; trypsin-EDTA is not recommended since EXO CD contains no serum to quench trypsin activity)
• Incubation conditions: 37 °C, 5% CO₂, humidified.

Product information & packaging

• Product name: CellCor™ EXO CD (Cat. No.: YSP017) – pack size 500 mL.
• Storage: 2–8 °C; shelf life 6 months.
• Target cells: hMSC;
• Applications: hMSC expansion & exosome collection.
• QC: Mycoplasma, Sterility, Endotoxin, Particle Count.

Quick guide (step-by-step)

Media Change (summary): Thaw → expand (to 75–85%) → discard supernatant → (optional) wash with EXO CD → replace with EXO CD → collect CM at 85–90% → isolate → (optional) replace with fresh EXO CD after 24–48 h for additional collection.

Seeding with EXO CD: Expand in MSC CD AOF → reseed in EXO CD → collect CM at 85–90% → (optional) replace EXO CD after 24–48 h → isolate.

Thawing with EXO CD: Prepare EXO CD in BSC → thaw → resuspend at 1:9 (medium:pellet) → centrifuge at 200–300 × g for 3 min → seed & incubate at 37 °C / 5% CO₂ → passage at 75–85% → collect CM at 85–90% → (optional) replace after 24–48 h.

Frequently Asked Questions (FAQ)

1) What is the difference between EXO CD and MSC CD AOF?
MSC CD AOF is optimized for hMSC expansion, while EXO CD is optimized for exosome collection. They can be combined in a workflow: MSC expansion with MSC CD AOF → exosome collection with EXO CD.

2) Why is TrypLE™ Express recommended instead of trypsin-EDTA?
EXO CD does not contain serum to neutralize trypsin. TrypLE™ Express enables safer handling without a serum quenching step.

3) When is washing recommended before switching to EXO CD?
Washing with EXO CD can be performed to completely remove residual conventional/serum-containing media and reduce protein contaminants prior to CM collection.

4) Can antibiotics be added?
Yes. Gentamicin or Penicillin–Streptomycin may be used as recommended.

5) Does CM need to be concentrated before functional assays?
In the illustrated examples, CM was concentrated 100× by TFF prior to functional evaluation.

Implementation suggestions

• Standardize an end-to-end EVs workflow: MSC expansion (MSC CD AOF) → EXO CD (CM collection) → TFF concentration / automated EV isolation → QC (NTA, BCA, mycoplasma, endotoxin).
• Integrate equipment and consumables within the Biogroup portfolio (CO₂ incubators, exosome isolation systems, TFF/UF systems, NTA/BCA kits, sample storage solutions) to deliver a comprehensive solution for R&D–QC–pilot applications.

Ordering & technical support

• Cat. No.: YSP017 – 500 mL/bottle, stored at 2–8 °C
• Technical/application support & lab demo: Contact Biogroup Vietnam for customized, standardized exosome collection workflows tailored to your project requirements (R&D, QC, pre-manufacturing).