Sepapure Resins for Tag-Affinity Chromatography

Description

FPLC media based on cross-linked agarose beads with a mean diameter of 100 μm. The FPLC media is functionalized with NTA ligands for His-Tag and glutathione ligands for GST-Tag purifications.

Properties:

Sepapure® Affinity media for recombinantly tagged proteins is designed to be used with most aqueous buffer systems. It is long lasting when correctly handled and compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery.

Technical data:

Agarose beads with typical loading ranges of 1 – 40 mg/l (Ni-NTA) or 1 – 10 mg/ml (Glutathione). The maximum operating pressure the Sepapure® columns should be used at is 3 bar, while the recommended flowrate is 1 CV/ml.

Recommended application areas:

Typically used in the first step („capture“) of an FPLC purification procedure.

Order information:

Available as prepacked 1 ml or 5 ml cartridge or as bulk material.

• Sepapure FF Ni-NTA for His-tag purification
• Sepapure FF4 Glutathione for GST-tag purification
• Other packaging sizes (optional): 25 mL, 100 mL, 250 mL, 500 mL and 1000 mL.