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Description
STEMprep™ Tissue Dissociator
STEMprep™ is a benchtop system flexible enough to fit your lab’s various tissue processing needs. Its innovative design features independent temperature-controlled sample slots that enable tailored dissociation conditions for each sample.
An intuitive touchscreen interface simplifies operation.

Figure 1. Overview of the STEMprep™ Tissue Dissociator System

Figure 2. Compact Design of the STEMprep™ Primary Unit
Flexible by design, STEMprep™ meets diverse research needs with modular solutions.
The Primary Unit can process up to four samples simultaneously or in parallel. As your throughput requirements increase, the system can be expanded to accommodate 8, 12, or even more samples at a time.
Why Use STEMprep™?
- AUTOMATED & RELIABLE. Standardize tissue dissociation with automation for consistent, reproducible results.
- HIGH YIELD & VIABILITY. Obtain high numbers of viable single cells with preserved functionality for downstream applications.
- BUILT-IN HEATING & COOLING. Ensure optimal sample processing for various sample types and applications with built-in temperature control from 4 to 37°C.
- MODULAR & SCALABLE. Choose from a 4-sample, 8-sample, or 12-sample system—scaling seamlessly to meet increasing throughput needs while maintaining efficiency.
- FLEXIBLE & CUSTOMIZABLE. Use validated STEMCELL protocols or create your own to adapt STEMprep™ to different tissue types and experimental needs.
>> For detailed operating instructions, refer to the Technical Manual (Document #10000030598) or Quick Start Guide (Document #27273).
System Performance with STEMprep™
High Yields – Preserved Viability – Reliable Results
Achieve consistent dissociation of diverse tissue types by minimizing user variability. By adding STEMprep™ to your workflow, you can reduce manual error, automate complex steps, and improve reproducibility.
The integrated cold-hold functionality maintains low temperatures after protocol completion, offering added flexibility for sample retrieval and supporting downstream workflows such as RNA-based analyses where temperature control is critical.
Figure 3. The STEMprep™ Tissue Dissociator System Enables Reliable Automated Mouse Tissue Dissociation.
Mouse tissues were dissociated into single-cell suspensions using the STEMprep™ Tissue Dissociator System and the STEMprep™ Tissue Dissociation Kits. Viability and yield of single-cell suspensions generated from (A) 4T1 mammary tumors (n = 10), (B) B16 melanoma tumors (n = 28), (C) brain (n = 68), (D) liver (n = 37), (E) lung (n = 64), and (F) spleen (n = 35) were measured.
Primary solid tumors were generated by subcutaneous injection of tumor cells into the flanks of mice. Tumor, liver, and lung samples were treated with ammonium chloride solution to lyse red blood cells. Brain samples were processed with 18% v/v OptiPrep™ to remove myelin and cell debris prior to analysis. Cell viability and yield following STEMprep™ processing were assessed by flow cytometry. Data are presented as mean ± SD.
Figure 4. STEMprep™ Cold Temperature Hold Feature Preserves Mouse Lung and Spleen Cell Viability.
Mouse lung and spleen tissues were dissociated using the STEMprep™ Mouse Lung and Spleen Dissociation Kits, respectively. After the protocol run was completed, the samples were held on the STEMprep™ Tissue Dissociator for up to 24 hours at 4°C, 22°C, and 37°C. Viability of total nucleated (A) lung and (B) spleen cells. Data are presented as mean ± SD (n = 3).
STEMprep™ Sample Tubes

Designed specifically for use with the STEMprep™ Tissue Dissociator and associated tissue dissociation kits, STEMprep™ Sample Tubes help users reduce variability and save time, enabling standardized dissociation of a wide range of tissue types.
Each tube features an integrated rotor and blade system that is activated by the instrument to dissociate tissue samples. The design ensures consistent, high-quality results across experiments— making it ideal for downstream applications like single-cell analysis or cell-based assays.
STEMprep™ Sample Tubes are ready to use right out of the package. Simply add your sample and reagents, secure the lid, and load the tube into the instrument to begin your dissociation protocol. The single-use design minimizes contamination risk, while the robust construction ensures functionality at the instrument’s maximum operating speed and revolutions.
Seamless Integration with EasySep™ and ImmunoCult™
The STEMprep™ Tissue Dissociator system works seamlessly with downstream tools that researchers rely on, such as for immune profiling, functional assays, and cell culture.
- After dissociation, cells are ready for immediate use with EasySep™, STEMCELL’s fast and column-free immunomagnetic cell separation system.
- ImmunoCult™ – a suite of reagents for efficient cell activation, expansion, and differentiation.
This integration creates a complete, optimized, and efficient workflow—from tissue dissociation to functional cell analysis.
Learn more about system performance: Automate Tissue Processing with STEMprep™ for Versatile Sample Preparation
Watch the product video: Elevate Tissue Dissociation with STEMprep™
>> Learn more about system performance: Automate Tissue Processing with STEMprep™ for Versatile Sample Preparation
>> Watch the machine introduction video: Elevate Tissue Dissociation with STEMprep™
Additional information
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