FPLC media based on cross-linked agarose beads with a mean diameter of 100 μm. The FPLC media is functionalized either with Protein A or Protein G ligands for the binding of antibodies or antibody fragments.

Properties:

Sepapure® Affinity media for antibodies or antibody fragments is designed to be used with most aqueous buffer systems. It is long lasting when correctly handled and are compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery.

Technical data:

Agarose beads with typical loading ranges of 1 - 30 mg/l (Protein A column) or 1 - 15 mg/ml (Protein G column). The maximum operating pressure the Sepapure® columns should be used at is 3 bar, while the recommended flowrate is 1 CV/ml.

Recommended application areas:

Typically used in the first step “capture” of an FPLC purification procedure.

Order information:

Available as prepacked 1 ml or 5 ml cartridge or as bulk material.

• Sepapure Protein A FF prepacked for antibody purification.
• Sepapure Protein G FF 5 prepacked for antibody purification.
• Other packaging sizes (optional): 5 mL, 10 mL, 25 mL, 50 mL, 250 mL and 1000 mL.

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