FPLC media based on cross-linked agarose beads with a mean diameter of 100 μm. The FPLC media is functionalized with different linkers ranging from strong anion exchange ligands to weak cation exchange ligands.

Properties:

Sepapure® Ion-Exchange media is designed to be used with most aqueous buffer systems. It is long lasting when correctly handled and is compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery.

Technical data:

The agarose beads are functionalized with a quaternary ammonium for the strong anion exchanger (Q) and with diethylaminoethyl for the weak anion exchanger (DEAE). The strong cation exchanger is functionalized with sulphopropyl (SP) and the weak cation exchanger with carboxymethyl (CM). The maximum operating pressure the Sepapure® columns should be used at is 3 bar, while the recommended flowrate is 1 CV/ml.

Recommended application areas:

Typically used in the intermediate step of an FPLC purification procedure.

Order information:

Available as prepacked 1 ml or 5 ml cartridge or as bulk material.

• Sepapure Q FF6 Resin, strong anion exchanger
• Sepapure DEAE FF6 Resin, weak anion exchange
• Sepapure CM FF6 Resin, weak cation exchanger
• Sepapure SP FF6 Resin, strong cation exchanger
• Other packaging sizes (optional): 25 mL, 100 mL, 500 mL and 1000 mL.

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