Spectrophotometers vs. Fluorometers: Choosing the Right Tool for DNA/RNA Quantification
Sample quantification is crucial for accurate molecular analysis. This guide…
Comprehensive Nucleic Acid Quality Control-Total Solutions for Molecular Experiment Issues
Comprehensive Nucleic Acid Quality Control-Total Solutions for Molecular Experiment Issues
Nucleic acid quality control is the foundation of molecular experiments!
To the scientific researchers in the molecular laboratories, the nucleic acid quality directly influences the success of downstream experiments. Without a doubt, nucleic acids with high concentration, purity and whole fragment are the ideal nucleic acid samples to laboratory fellows. The target nucleic acids from tissues, cells and microorganisms are purified and enriched. Prior to handling the nucleic acid sample further, the quality control of nucleic acid concentration, purity and fragment integrity shall be performed. Specifically speaking, the concentration measurement is to meet the sample requirement in the downstream experiments. The purity measurement is to confirm whether the sample is contaminated by other nucleic acids, proteins or chemical substances. The fragment integrity is the key factor to evaluate whether or not the sample has degraded.
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