Clinical-Grade MSC-EV production: The role of CD, AOF, and GMP conditions

CD and AOF media are increasingly becoming a top priority in MSC and MSC-EV production, especially as GMP requirements and clinical validation standards grow more stringent. Many customers raise important questions: What exactly are chemical-defined (CD) and animal-origin-free (AOF) media? How do they differ from traditional serum-containing media used for MSC culture? And is it mandatory to adopt CD/AOF media in MSC-EV manufacturing processes?

A clear understanding of the nature and role of CD and AOF media is the first critical step toward building a stable, safe, and clinically ready cell culture process.