SepMate™ for Isolation of Mononuclear Cells (MNCs)

Procedures/ Directions for Use February 25, 2024

SepMate™ is an innovative tube that enables consistent and hassle-free peripheral blood mononuclear cell (PBMC) isolation in just 15 minutes. The typical PBMC isolation protocol is laborious, requiring slow layering of blood on density gradient medium (e.g. Lymphoprep™, Ficoll-Paque™), lengthy 30-minute centrifugation with the brake off, and careful harvest of PBMCs using a pipette. SepMate™ simplifies and speeds up the PBMC isolation protocol to an easy 15-minute procedure. The SepMate™ insert allows users to quickly layer blood over the density gradient medium and prevents the layers from mixing. Centrifugation is performed with the brake on, after which isolated PBMCs are simply poured off into a fresh tube.